LOCUS       p15A-ΦX174-F2           5220 bp DNA     circular SYN 19-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   123
  TITLE     Direct Submission
  JOURNAL   Exported Feb 24, 2025 from SnapGene 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1..1786
                     /host="Enterobacteriaceae bacterium bta3-1"
                     /strain="bta3-1"
                     /mol_type="genomic DNA"
                     /country="USA"
                     /isolation_source="honeybee gut"
                     /db_xref="taxon:10847"
                     /organism="Escherichia virus phiX174"
     source          1..1786
                     /host="Enterobacteriaceae bacterium bta3-1"
                     /strain="bta3-1"
                     /mol_type="genomic DNA"
                     /country="USA"
                     /isolation_source="honeybee gut"
                     /db_xref="taxon:10847"
                     /organism="Escherichia virus phiX174"
     source          1793..5214
                     /host="Enterobacteriaceae bacterium bta3-1"
                     /strain="bta3-1"
                     /mol_type="genomic DNA"
                     /country="USA"
                     /isolation_source="honeybee gut"
                     /db_xref="taxon:10847"
                     /organism="Escherichia virus phiX174"
     rep_origin      complement(156..701)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     misc_feature    802..852
                     /label=HA for TnpA/phiC31
     terminator      815..909
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(930..1589)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1590..1692)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
     CDS             complement(1722..1786)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="FGLELIICGLEKQLKCESGS"
     misc_feature    1722..1771
                     /label=HA for TnpA/phiC31
     misc_feature    1787..1792
                     /label=BstZ171
     misc_feature    1793..5214
                     /label=BAC-Phi-X174-F2
     CDS             1793..1960
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="F652_4288"
                     /product="protein D"
                     /label=F652_4288/D
                     /note="external scaffolding protein"
                     /protein_id="AJR02267.1"
                     /translation="MEGAEFTENIINGVERPVKAAELFAFTLRVRAGNTDVLTDAEENV
                     RQKLRAEGVM"
     gene            1793..1960
                     /locus_tag="F652_4288"
                     /label=F652_4288
     CDS             1793..1955
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="F652_4293"
                     /product="protein E"
                     /label=F652_4293/E
                     /note="cell lysis protein"
                     /protein_id="AJR02268.1"
                     /translation="WKALNLRKTLLMASSVRLKPLNCSRLPCVYAQETLTFLLTQKKTC
                     VKNYVQKE"
     gene            1793..1955
                     /locus_tag="F652_4293"
                     /label=F652_4293
     CDS             1960..2076
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="F652_4298"
                     /product="protein J"
                     /label=F652_4298/J
                     /note="core protein"
                     /protein_id="AJR02269.1"
                     /translation="MSKGKKRSGARPGRPQPLRGTKGKRKGARLWYVGGQQF"
     gene            1960..2076
                     /locus_tag="F652_4298"
                     /label=F652_4298
     CDS             2113..3396
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="F652_4303"
                     /product="protein F"
                     /label=F652_4303/F
                     /note="capsid protein"
                     /protein_id="AJR02270.1"
                     /translation="MSNIQTGAERMPHDLSHLGFLAGQIGRLITISTTPVIAGDSFEMD
                     AVGALRLSPLRRGLAIDSTVDIFTFYVPHRHVYGEQWIKFMKDGVNATPLPTVNTTGYI
                     DHAAFLGTINPDTNKIPKHLFQGYLNIYNNYFKAPWMPDRTEANPNELNQDDARYGFRC
                     CHLKNIWTAPLPPETELSRQMTTSTTSIDIMGLQAAYANLHTDQERDYFMQRYHDVISS
                     FGGKTSYDADNRPLLVMRSNLWASGYDVDGTDQTSLGQFSGRVQQTYKHSVPRFFVPEH
                     GTMFTLALVRFPPTATKEIQYLNAKGALTYTDIAGDPVLYGNLPPREISMKDVFRSGDS
                     SKKFKIAEGQWYRYAPSYVSPAYHLLEGFPFIQEPPSGDLQERVLIRHHDYDQCFQSVQ
                     LLQWNSQVKFNVTVYRNLPTTRDSIMTS"
     gene            2113..3396
                     /locus_tag="F652_4303"
                     /label=F652_4303
     CDS             3507..4034
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="F652_4308"
                     /product="protein G"
                     /label=F652_4308/G
                     /note="major spike protein"
                     /protein_id="AJR02271.1"
                     /translation="MFQTFISRHNSNFFSDKLVLTSVTPASSAPVLQTPKATSSTLYFD
                     SLTVNAGNGGFLHCIQMDTSVNAANQVVSVGADIAFDADPKFFACLVRFESSSVPTTLP
                     TAYDVYPLDGRHDGGYYTVKDCVTIDVLPRTPGNNVYVGFMVWSNFTATKCRGLVSLNQ
                     VIKEIICLQPLK"
     gene            3507..4034
                     /locus_tag="F652_4308"
                     /label=F652_4308
     CDS             4043..5029
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="F652_4313"
                     /product="protein H"
                     /label=F652_4313/H
                     /note="minor spike protein"
                     /protein_id="AJR02272.1"
                     /translation="MFGAIAGGIASALAGGAMSKLFGGGQKAASGGIQGDVLATDNNTV
                     GMGDAGIKSAIQGSNVPNPDEAVPSFVSGAMAKAGKGLLEGTLQAGTSAVSDKLLDLVG
                     LGGKSAADKGKDTRDYLAAAFPELNAWERAGADASSAGMVDAGFENQKELTKMQLDNQK
                     EIAEMQNETQKEIAGIQSATSRQNTKDQVYAQNEMLAYQQKESTARVASIMENTNLSKQ
                     QQVSEIMRQMLTQAQTAGQYFTNDQIKEMTRKVSAEVDLVHQQTQNQRYGSSHIGATAK
                     DISNVVTDAASGVVDIFHGIDKAVADTWNNFWKDGKADGIGSNLSRK"
     gene            4043..5029
                     /locus_tag="F652_4313"
                     /label=F652_4313
     misc_feature    5010..5029
                     /label=HLA-in article
     CDS             5093..5214
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="F652_4273"
                     /product="protein A"
                     /label=F652_4273/A
                     /note="DNA replication initiation protein"
                     /protein_id="AJR02264.1"
                     /translation="MVRSYYPSECHADYFDFERIEALKPAIEACGISTLSQSPM"
     gene            5093..5214
                     /locus_tag="F652_4273"
                     /label=F652_4273
     misc_feature    5215..5220
                     /label=BstZ171
ORIGIN
        1 cctagggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
       61 gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact
      121 taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac
      181 aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
      241 taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt
      301 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc
      361 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
      421 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc
      481 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt
      541 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt
      601 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt
      661 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc
      721 agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca
      781 gccccatacg atataagttg ttactagtgc ttggattctc accaataaaa aacgcccggc
      841 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
      901 acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac
      961 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
     1021 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     1081 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     1141 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     1201 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     1261 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     1321 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga
     1381 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     1441 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     1501 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     1561 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     1621 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     1681 acgtgccgat caacgtctca ttttcgccag atatcgacgt cttaagaccc actttcacat
     1741 ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaatagtat acatggaagg
     1801 cgctgaattt acggaaaaca ttattaatgg cgtcgagcgt ccggttaaag ccgctgaatt
     1861 gttcgcgttt accttgcgtg tacgcgcagg aaacactgac gttcttactg acgcagaaga
     1921 aaacgtgcgt caaaaattac gtgcagaagg agtgatgtaa tgtctaaagg taaaaaacgt
     1981 tctggcgctc gccctggtcg tccgcagccg ttgcgaggta ctaaaggcaa gcgtaaaggc
     2041 gctcgtcttt ggtatgtagg tggtcaacaa ttttaattgc aggggcttcg gccccttact
     2101 tgaggataaa ttatgtctaa tattcaaact ggcgccgagc gtatgccgca tgacctttcc
     2161 catcttggct tccttgctgg tcagattggt cgtcttatta ccatttcaac tactccggtt
     2221 atcgctggcg actccttcga gatggacgcc gttggcgctc tccgtctttc tccattgcgt
     2281 cgtggccttg ctattgactc tactgtagac atttttactt tttatgtccc tcatcgtcac
     2341 gtttatggtg aacagtggat taagttcatg aaggatggtg ttaatgccac tcctctcccg
     2401 actgttaaca ctactggtta tattgaccat gccgcttttc ttggcacgat taaccctgat
     2461 accaataaaa tccctaagca tttgtttcag ggttatttga atatctataa caactatttt
     2521 aaagcgccgt ggatgcctga ccgtaccgag gctaacccta atgagcttaa tcaagatgat
     2581 gctcgttatg gtttccgttg ctgccatctc aaaaacattt ggactgctcc gcttcctcct
     2641 gagactgagc tttctcgcca aatgacgact tctaccacat ctattgacat tatgggtctg
     2701 caagctgctt atgctaattt gcatactgac caagaacgtg attacttcat gcagcgttac
     2761 catgatgtta tttcttcatt tggaggtaaa acctcttatg acgctgacaa ccgtccttta
     2821 cttgtcatgc gctctaatct ctgggcatct ggctatgatg ttgatggaac tgaccaaacg
     2881 tcgttaggcc agttttctgg tcgtgttcaa cagacctata aacattctgt gccgcgtttc
     2941 tttgttcctg agcatggcac tatgtttact cttgcgcttg ttcgttttcc gcctactgcg
     3001 actaaagaga ttcagtacct taacgctaaa ggtgctttga cttataccga tattgctggc
     3061 gaccctgttt tgtatggcaa cttgccgccg cgtgaaattt ctatgaagga tgttttccgt
     3121 tctggtgatt cgtctaagaa gtttaagatt gctgagggtc agtggtatcg ttatgcgcct
     3181 tcgtatgttt ctcctgctta tcaccttctt gaaggcttcc cattcattca ggaaccgcct
     3241 tctggtgatt tgcaagaacg cgtacttatt cgccaccatg attatgacca gtgtttccag
     3301 tccgttcagt tgttgcagtg gaatagtcag gttaaattta atgtgaccgt ttatcgcaat
     3361 ctgccgacca ctcgcgattc aatcatgact tcgtgataaa agattgagtg tgaggttata
     3421 acgccgaagc ggtaaaaatt ttaatttttg ccgctgaggg gttgaccaag cgaagcgcgg
     3481 taggttttct gcttaggagt ttaatcatgt ttcagacttt tatttctcgc cataattcaa
     3541 actttttttc tgataagctg gttctcactt ctgttactcc agcttcttcg gcacctgttt
     3601 tacagacacc taaagctaca tcgtcaacgt tatattttga tagtttgacg gttaatgctg
     3661 gtaatggtgg ttttcttcat tgcattcaga tggatacatc tgtcaacgcc gctaatcagg
     3721 ttgtttctgt tggtgctgat attgcttttg atgccgaccc taaatttttt gcctgtttgg
     3781 ttcgctttga gtcttcttcg gttccgacta ccctcccgac tgcctatgat gtttatcctt
     3841 tggatggtcg ccatgatggt ggttattata ccgtcaagga ctgtgtgact attgacgtcc
     3901 ttccccgtac gccgggcaat aatgtttatg ttggtttcat ggtttggtct aactttaccg
     3961 ctactaaatg ccgcggattg gtttcgctga atcaggttat taaagagatt atttgtctcc
     4021 agccacttaa gtgaggtgat ttatgtttgg tgctattgct ggcggtattg cttctgctct
     4081 tgctggtggc gccatgtcta aattgtttgg aggcggtcaa aaagccgcct ccggtggcat
     4141 tcaaggtgat gtgcttgcta ccgataacaa tactgtaggc atgggtgatg ctggtattaa
     4201 atctgccatt caaggctcta atgttcctaa ccctgatgag gccgtcccta gttttgtttc
     4261 tggtgctatg gctaaagctg gtaaaggact tcttgaaggt acgttgcagg ctggcacttc
     4321 tgccgtttct gataagttgc ttgatttggt tggacttggt ggcaagtctg ccgctgataa
     4381 aggaaaggat actcgtgatt atcttgctgc tgcatttcct gagcttaatg cttgggagcg
     4441 tgctggtgct gatgcttcct ctgctggtat ggttgacgcc ggatttgaga atcaaaaaga
     4501 gcttactaaa atgcaactgg acaatcagaa agagattgcc gagatgcaaa atgagactca
     4561 aaaagagatt gctggcattc agtcggcgac ttcacgccag aatacgaaag accaggtata
     4621 tgcacaaaat gagatgcttg cttatcaaca gaaggagtct actgctcgcg ttgcgtctat
     4681 tatggaaaac accaatcttt ccaagcaaca gcaggtttcc gagattatgc gccaaatgct
     4741 tactcaagct caaacggctg gtcagtattt taccaatgac caaatcaaag aaatgactcg
     4801 caaggttagt gctgaggttg acttagttca tcagcaaacg cagaatcagc ggtatggctc
     4861 ttctcatatt ggcgctactg caaaggatat ttctaatgtc gtcactgatg ctgcttctgg
     4921 tgtggttgat atttttcatg gtattgataa agctgttgcc gatacttgga acaatttctg
     4981 gaaagacggt aaagctgatg gtattggctc taatttgtct aggaaataac cgtcaggatt
     5041 gacaccctcc caattgtatg ttttcatgcc tccaaatctt ggaggctttt ttatggttcg
     5101 ttcttattac ccttctgaat gtcacgctga ttattttgac tttgagcgta tcgaggctct
     5161 taaacctgct attgaggctt gtggcatttc tactctttct caatccccaa tgctgtatac
//
