LOCUS       Exported                8230 bp ds-DNA     circular BCT 17-JAN-2025
DEFINITION  Escherichia coli strain DH5alpha pHA TST 3C SMO EABR clone HA TST 3C
            SMO EABR.
ACCESSION   BSeq#1
VERSION     .
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Gonzalez-Magaldi M, Gullapalli A, Papoulas O, Liu C, Leung AYH., Guo
            L, Brilot A, Marcotte EM, Ke Z, Leahy DJ.
  TITLE     Structure and organization of full-length Epidermal Growth Factor 
            Receptor in extracellular vesicles by cryo-electron tomography
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 8230)
  AUTHORS   Gonzalez-Magaldi M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2025) Department of Molecular Biosciences, 
            University of Texas at Austin, NHB 4.110, 100 E 24TH ST, Austin, 
            Texas 78712, United States
REFERENCE   3  (bases 1 to 8230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 24, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Bankit Comment: Vecscreen Comment:Submitter says that this sequence 
            is a Synthetic Construct
            Bankit Comment: TOTAL # OF SEQS:1
            Bankit Comment: ALT 
            EMAIL:mgonzalezmagaldi@utexas.edu,ldleahy@austin.utexas.edu.
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="Escherichia coli"
                     /plasmid="pHA TST 3C SMO EABR"
                     /strain="DH5alpha"
                     /mol_type="genomic DNA"
                     /isolation_source="plasmid"
                     /clone="HA TST 3C SMO EABR"
                     /note="synthetic plasmid; [cultured bacterial source]"
                     /db_xref="taxon:562"
     regulatory      235..614
                     /regulatory_class="enhancer"
                     /note="human cytomegalovirus immediate early enhancer; CMV 
                     enhancer"
     regulatory      615..818
                     /regulatory_class="promoter"
                     /label=CMV promoter
                     /note="CMV promoter"
     regulatory      863..881
                     /regulatory_class="promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase; T7 
                     promoter"
     misc_feature    936..941
                     /label=Kozac
                     /note="Kozac"
     misc_feature    942..1013
                     /label=endogenous signal peptide
                     /note="endogenous signal peptide"
     misc_feature    1026..1052
                     /product="YPYDVPDYA"
                     /label=HA (human influenza hemagglutinin) epitope tag
                     /note="HA (human influenza hemagglutinin) epitope tag"
     misc_feature    1071..1094
                     /product="WSHPQFEK"
                     /note="peptide that binds Strep-Tactin, an engineered form
                     of streptavidin; Strep-Tag II"
     misc_feature    1131..1154
                     /product="WSHPQFEK"
                     /note="peptide that binds Strep-Tactin, an engineered form
                     of streptavidin; Strep-Tag II"
     protein_bind    1161..1184
                     /label=protease binding site
                     /bound_moiety="protease"
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases; HRV 3C site"
     misc_feature    1197..3464
                     /product="SSGNATGPGPRSAGGSARRSAAVTGPPPPLSHCGRAAPCEPLRYNVCL 
                     GSVLPYGATSTLLAGDSDSQEEAHGKLVLWSGLRNAPRCWAVIQPLLCAVYMPKCEND 
                     RVELPSRTLCQATRGPCAIVERERGWPDFLRCTPDRFPEGCTNEVQNIKFNSSGQCEV 
                     PLVRTDNPKSWYEDVEGCGIQCQNPLFTEAEHQDMHSYIAAFGAVTGLCTLFTLATFV 
                     ADWRNSNRYPAVILFYVNACFFVGSIGWLAQFMDGARREIVCRADGTMRLGEPTSNET 
                     LSCVIIFVIVYYALMAGVVWFVVLTYAWHTSFKALGTTYQPLSGKTSYFHLLTWSLPF 
                     VLTVAILAVAQVDGDSVSGICFVGYKNYRYRAGFVLAPIGLVLIVGGYFLIRGVMTLF 
                     SIKSNHPGLLSEKAASKINETMLRLGIFGFLAFGFVLITFSCHFYDFFNQAEWERSFR 
                     DYVLCQANVTIGLPTKQPIPDCEIKNRPSLLVEKINLFAMFGTGIAMSTWVWTKATLL 
                     IWRRTWCRLTGQSDDEPKRIKKSKMIAKAFSKRHELLQNPGQELSFSMHTVSHDGPVA 
                     GLAFDLNEPSADVSSAWAQHVTKMVARRGAILPQDISVTPVATPVPPEEQANLWLVEA 
                     EISPELQKRLGRKKKRRKRKKEVCPLAPPPELHPPAPAPSTIPRLPQLPRQKCLVAAG 
                     AWGAGDSCRQGAWTLVSNPFCPEPSPPQDPFLPSAPAPVAWAHGRRQGLGPIHSRTNL 
                     MDTELMDADSDF"
                     /label=SMO protein
                     /note="SMO protein"
     misc_feature    3465..3608
                     /product="ALPGNPDHREMGETLPEEVGEYRQPSGGSVPVSPGPPSGLEPTSSSPY
                     "
                     /label=Fcgr2 (fragment)
                     /note="Fcgr2 (fragment)"
     misc_feature    3621..3797
                     /product="FNSSINNIHEMEIQLKDALEKNQQWLVYDQQREVYVKGLLAKIFELEK 
                     KTETAAHSLP*"
                     /label=CEP55 (fragment)
                     /note="CEP55 (fragment)"
     regulatory      3830..4054
                     /regulatory_class="polyA_signal_sequence"
                     /note="bovine growth hormone polyadenylation signal; bGH
                     poly(A) signal"
     rep_origin      4100..4528
                     /direction=RIGHT
                     /note="f1 ori; f1 bacteriophage origin of replication;
                     arrow indicates direction of (+) strand synthesis"
     regulatory      4542..4871
                     /regulatory_class="promoter"
                     /note="SV40 enhancer and early promoter; SV40 promoter"
     rep_origin      4722..4857
                     /note="SV40 origin of replication; SV40 ori"
     gene            4938..5732
                     /gene="aph(3')-IIa"
                     /label=aph(3')-IIa
                     /note="confers resistance to neomycin, kanamycin, and
                     G418: Aminoglycoside phosphotransferase from Tn5"
     CDS             4938..5732
                     /codon_start=1
                     /transl_table=11
                     /gene="aph(3')-IIa"
                     /product="NeoR"
                     /label=aph(3')-IIa
                     /note="confers resistance to neomycin, kanamycin, and
                     G418: Aminoglycoside phosphotransferase from Tn5"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      5906..6027
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 polyadenylation signal; SV40 poly(A) signal"
     primer_bind     6076..6092
                     /note="common sequencing primer, one of multiple similar 
                     variants; M13 rev"
     protein_bind    6100..6116
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG).; lac 
                     operator"
     regulatory      complement(6124..6154)
                     /regulatory_class="promoter"
                     /note="promoter for the E. coli lac operon; lac promoter"
     protein_bind    6169..6190
                     /label=cAMP binding site
                     /bound_moiety="cAMP"
                     /note="CAP binding site"
     rep_origin      complement(6478..7063)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication; ori"
     gene            complement(7234..8094)
                     /gene="bla"
                     /label=bla
                     /note="beta-lactamase"
     CDS             complement(7234..8094)
                     /codon_start=1
                     /transl_table=11
                     /gene="bla"
                     /product="AmpR"
                     /label=bla
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     regulatory      complement(8095..8199)
                     /regulatory_class="promoter"
                     /label=AmpR promoter
                     /note="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta cccggatcct ctagagccac catgcgaccc tccgggacgg
      961 ccggggcagc gctcctggcg ctgctggctg cgctctgccc ggcgagtcgg gctggcggag
     1021 gcagctaccc atacgacgta ccagattacg ctggaggagg ctccagtgcg tggagtcatc
     1081 ctcaattcga gaaaggtgga ggttctggcg gtggatcggg aggttcagcg tggagccacc
     1141 cgcagttcga aaaatccgga cttgaagtcc tctttcaggg acccggcggg ggctcctcga
     1201 gcgggaacgc gaccgggcct gggcctcgga gcgcgggcgg gagcgcgagg aggagcgcgg
     1261 cggtgactgg ccctccgccg ccgctgagcc actgcggccg ggctgccccc tgcgagccgc
     1321 tgcgctacaa cgtgtgcctg ggctcggtgc tgccctacgg ggccacctcc acactgctgg
     1381 ccggagactc ggactcccag gaggaagcgc acggcaagct cgtgctctgg tcgggcctcc
     1441 ggaatgcccc ccgctgctgg gcagtgatcc agcccctgct gtgtgccgta tacatgccca
     1501 agtgtgagaa tgaccgggtg gagctgccca gccgtaccct ctgccaggcc acccgaggcc
     1561 cctgtgccat cgtggagagg gagcggggct ggcctgactt cctgcgctgc actcctgacc
     1621 gcttccctga aggctgcacg aatgaggtgc agaacatcaa gttcaacagt tcaggccagt
     1681 gcgaagtgcc cttggttcgg acagacaacc ccaagagctg gtacgaggac gtggagggct
     1741 gcggcatcca gtgccagaac ccgctcttca cagaggctga gcaccaggac atgcacagct
     1801 acatcgcggc cttcggggcc gtcacgggcc tctgcacgct cttcaccctg gccacattcg
     1861 tggctgactg gcggaactcg aatcgctacc ctgctgttat tctcttctac gtcaatgcgt
     1921 gcttctttgt gggcagcatt ggctggctgg cccagttcat ggatggtgcc cgccgagaga
     1981 tcgtctgccg tgcagatggc accatgaggc ttggggagcc cacctccaat gagactctgt
     2041 cctgcgtcat catctttgtc atcgtgtact acgccctgat ggctggtgtg gtttggtttg
     2101 tggtcctcac ctatgcctgg cacacttcct tcaaagccct gggcaccacc taccagcctc
     2161 tctcgggcaa gacctcctac ttccacctgc tcacctggtc actccccttt gtcctcactg
     2221 tggcaatcct tgctgtggcg caggtggatg gggactctgt gagtggcatt tgttttgtgg
     2281 gctacaagaa ctaccgatac cgtgcgggct tcgtgctggc cccaatcggc ctggtgctca
     2341 tcgtgggagg ctacttcctc atccgaggag tcatgactct gttctccatc aagagcaacc
     2401 accccgggct gctgagtgag aaggctgcca gcaagatcaa cgagaccatg ctgcgcctgg
     2461 gcatttttgg cttcctggcc tttggctttg tgctcattac cttcagctgc cacttctacg
     2521 acttcttcaa ccaggctgag tgggagcgca gcttccggga ctatgtgcta tgtcaggcca
     2581 atgtgaccat cgggctgccc accaagcagc ccatccctga ctgtgagatc aagaatcgcc
     2641 cgagccttct ggtggagaag atcaacctgt ttgccatgtt tggaactggc atcgccatga
     2701 gcacctgggt ctggaccaag gccacgctgc tcatctggag gcgtacctgg tgcaggttga
     2761 ctgggcagag tgacgatgag ccaaagcgga tcaagaagag caagatgatt gccaaggcct
     2821 tctctaagcg gcacgagctc ctgcagaacc caggccagga gctgtccttc agcatgcaca
     2881 ctgtgtccca cgacgggccc gtggcgggct tggcctttga cctcaatgag ccctcagctg
     2941 atgtctcctc tgcctgggcc cagcatgtca ccaagatggt ggctcggaga ggagccatac
     3001 tgccccagga tatttctgtc acccctgtgg caactccagt gcccccagag gaacaagcca
     3061 acctgtggct ggttgaggca gagatctccc cagagctgca gaagcgcctg ggccggaaga
     3121 agaagaggag gaagaggaag aaggaggtgt gcccgctggc gccgccccct gagcttcacc
     3181 cccctgcccc tgcccccagt accattcctc gactgcctca gctgccccgg cagaaatgcc
     3241 tggtggctgc aggtgcctgg ggagctgggg actcttgccg acagggagcg tggaccctgg
     3301 tctccaaccc attctgccca gagcccagtc cccctcagga tccatttctg cccagtgcac
     3361 cggcccccgt ggcatgggct catggccgcc gacagggcct ggggcctatt cactcccgca
     3421 ccaacctgat ggacacagaa ctcatggatg cagactcgga cttcgctttg cctggcaatc
     3481 ccgatcacag ggaaatgggg gaaactctcc cagaagaggt tggagaatat cggcagccat
     3541 ctggtggcag tgtacctgtg tctccgggtc cgcccagtgg tcttgaaccc actagctcct
     3601 caccttacgg cggcggctcc tttaacagct caatcaacaa catccacgaa atggagattc
     3661 agctgaagga tgcactcgaa aagaatcaac aatggctcgt ttacgaccaa caacgcgagg
     3721 tgtacgtgaa aggattgctt gcaaaaattt tcgaactgga aaagaaaaca gagactgcag
     3781 cacatagtct tccttgaagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     3841 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     3901 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     3961 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     4021 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     4081 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4141 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4201 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4261 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4321 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4381 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4441 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     4501 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     4561 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4621 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4681 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     4741 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     4801 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     4861 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     4921 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     4981 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     5041 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     5101 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     5161 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     5221 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     5281 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     5341 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     5401 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     5461 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     5521 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     5581 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     5641 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     5701 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     5761 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     5821 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     5881 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     5941 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6001 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     6061 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6121 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6181 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6241 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6301 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6361 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6421 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6481 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6541 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6601 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6661 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6721 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6781 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6841 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6901 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6961 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt
     7021 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7081 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7141 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7201 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7261 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7321 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7381 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7441 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7501 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7561 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7621 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7681 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7741 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7801 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7861 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7921 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7981 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8041 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     8101 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     8161 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     8221 acctgacgtc
//
