LOCUS       Exported                9441 bp ds-DNA     circular BCT 27-JAN-2025
DEFINITION  Escherichia coli strain DH5alpha pstreptagII 3C EGFR EABR clone 
            streptagII 3C EGFR EABR.
ACCESSION   BSeq#1
VERSION     .
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
REFERENCE   1  (bases 1 to 9441)
  AUTHORS   Gonzalez-Magaldi M, Gullapalli A, Papoulas O, Liu C, Leung AYH., Guo
            L, Brilot A, Marcotte EM, Ke Z, Leahy DJ.
  TITLE     Structure and organization of full-length Epidermal Growth Factor 
            Receptor in extracellular vesicles by cryo-electron tomography
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 9441)
  AUTHORS   Gonzalez-Magaldi M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2025) Department of Molecular Biosciences, 
            University of Texas at Austin, NHB 4.110, 100 E 24TH ST, Austin, 
            Texas 78712, United States
REFERENCE   3  (bases 1 to 9441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 24, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Bankit Comment: Vecscreen Comment:Submitter says that this sequence 
            is a Synthetic Construct
            Bankit Comment: TOTAL # OF SEQS:1
            Bankit Comment: ALT 
            EMAIL:mgonzalezmagaldi@utexas.edu,ldleahy@austin.utexas.edu.
FEATURES             Location/Qualifiers
     source          1..9441
                     /organism="Escherichia coli"
                     /plasmid="pstreptagII 3C EGFR EABR"
                     /strain="DH5alpha"
                     /mol_type="genomic DNA"
                     /country="USA: Texas"
                     /isolation_source="synthetic plasmid"
                     /clone="streptagII 3C EGFR EABR"
                     /label=[cultured bacterial source]
                     /note="[cultured bacterial source]"
                     /db_xref="taxon:562"
     regulatory      43..267
                     /regulatory_class="polyA_signal_sequence"
                     /note="bovine growth hormone polyadenylation signal; bGH
                     poly(A) signal"
     rep_origin      313..741
                     /direction=RIGHT
                     /note="f1 ori; f1 bacteriophage origin of replication;
                     arrow indicates direction of (+) strand synthesis"
     regulatory      755..1084
                     /regulatory_class="promoter"
                     /note="SV40 enhancer and early promoter; SV40 promoter"
     rep_origin      935..1070
                     /note="SV40 origin of replication; SV40 ori"
     gene            1151..1945
                     /gene="aph(3')-IIa"
                     /label=aph(3')-IIa
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
     CDS             1151..1945
                     /codon_start=1
                     /transl_table=11
                     /gene="aph(3')-IIa"
                     /product="NeoR"
                     /label=aph(3')-IIa
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      2119..2240
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 polyadenylation signal; SV40 poly(A) signal"
     primer_bind     complement(2289..2305)
                     /note="common sequencing primer, one of multiple similar 
                     variants; M13 rev"
     protein_bind    2313..2329
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG).; lac 
                     operator"
     regulatory      complement(2337..2367)
                     /regulatory_class="promoter"
                     /label=lac promoter
                     /note="lac promoter"
     protein_bind    2382..2403
                     /label=cAMP binding site
                     /bound_moiety="cAMP"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2691..3276)
                     /direction=LEFT
                     /note="ori; high-copy-number ColE1/pMB1/pBR322/pUC origin
                     of replication"
     gene            complement(3447..4412)
                     /gene="bla"
                     /label=bla
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
     CDS             complement(3447..4307)
                     /codon_start=1
                     /transl_table=11
                     /gene="bla"
                     /product="AmpR"
                     /label=bla
                     /note="beta-Lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     regulatory      complement(4308..4412)
                     /regulatory_class="promoter"
                     /gene="bla"
                     /gene="bla"
                     /label=AmpR promoter
                     /note="AmpR promoter"
     regulatory      4678..5057
                     /regulatory_class="enhancer"
                     /note="human cytomegalovirus immediate early enhancer; CMV 
                     enhancer"
     regulatory      5058..5261
                     /regulatory_class="promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter; CMV promoter"
     regulatory      5306..5324
                     /regulatory_class="promoter"
                     /note="T7 promoter; promoter for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    5379..5384
                     /label=Kozak sequence
                     /note="Kozak sequence"
     misc_feature    5385..5456
                     /note="ECD EGFR; extracellular domain of EGFR receptor"
     misc_feature    5505..5528
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases; HRV 3C site"
     misc_feature    7392..7484
                     /label=tm of EGFR
                     /note="tm of EGFR"
     misc_feature    7395..9098
                     /label=ICD of EGFR
                     /note="ICD of EGFR"
     misc_feature    9108..9251
                     /label=Fcgr2 (fragment)
                     /note="Fcgr2 (fragment)"
     misc_feature    9252..9263
                     /label=linker 3x(4xGly-Ser)
                     /note="linker 3x(4xGly-Ser)"
     misc_feature    9264..9437
                     /label=CEP55 (fragment)
                     /note="CEP55 (fragment)"
ORIGIN
        1 tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
       61 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
      121 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
      181 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
      241 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
      301 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
      361 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
      421 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
      481 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
      541 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
      601 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
      661 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
      721 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
      781 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
      841 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
      901 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      961 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1021 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     1081 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     1141 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     1201 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     1261 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     1321 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     1381 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     1441 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     1501 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     1561 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     1621 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     1681 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     1741 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     1801 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     1861 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     1921 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     1981 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     2041 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     2101 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2161 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     2221 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     2281 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     2341 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     2401 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     2461 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     2521 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2581 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2641 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2701 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2761 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2821 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2881 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2941 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3001 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3061 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3121 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3181 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt ttttgtttgc
     3241 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3301 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3361 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3421 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3481 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3541 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3601 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3661 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3721 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3781 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3841 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3901 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3961 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4021 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4081 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4141 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4201 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4261 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4321 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4381 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     4441 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     4501 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     4561 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     4621 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     4681 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     4741 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     4801 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     4861 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     4921 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     4981 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     5041 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     5101 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     5161 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     5221 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     5281 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     5341 agcgtttaaa cttaagcttg gtacccggat cctctagagc caccatgcga ccctccggga
     5401 cggccggggc agcgctcctg gcgctgctgg ctgcgctctg cccggcgagt cgggctggcg
     5461 gaggcagctg gagccacccg cagttcgaaa agggaggagg ctcccttgaa gtcctctttc
     5521 agggacccgg cgggggctcc ctggaggaaa agaaagtttg ccaaggcacg agtaacaagc
     5581 tcacgcagtt gggcactttt gaagatcatt ttctcagcct ccagaggatg ttcaataact
     5641 gtgaggtggt ccttgggaat ttggaaatta cctatgtgca gaggaattat gatctttcct
     5701 tcttaaagac catccaggag gtggctggtt atgtcctcat tgccctcaac acagtggagc
     5761 gaattccttt ggaaaacctg cagatcatca gaggaaatat gtactacgaa aattcctatg
     5821 ccttagcagt cttatctaac tatgatgcaa ataaaaccgg actgaaggag ctgcccatga
     5881 gaaatttaca ggaaatcctg catggcgccg tgcggttcag caacaaccct gccctgtgca
     5941 acgtggagag catccagtgg cgggacatag tcagcagtga ctttctcagc aacatgtcga
     6001 tggacttcca gaaccacctg ggcagctgcc aaaagtgtga tccaagctgt cccaatggga
     6061 gctgctgggg tgcaggagag gagaactgcc agaaactgac caaaatcatc tgtgcccagc
     6121 agtgctccgg gcgctgccgt ggcaagtccc ccagtgactg ctgccacaac cagtgtgctg
     6181 caggctgcac aggcccccgg gagagcgact gcctggtctg ccgcaaattc cgagacgaag
     6241 ccacgtgcaa ggacacctgc cccccactca tgctctacaa ccccaccacg taccagatgg
     6301 atgtgaaccc cgagggcaaa tacagctttg gtgccacctg cgtgaagaag tgtccccgta
     6361 attatgtggt gacagatcac ggctcgtgcg tccgagcctg tggggccgac agctatgaga
     6421 tggaggaaga cggcgtccgc aagtgtaaga agtgcgaagg gccttgccgc aaagtgtgta
     6481 acggaatagg tattggtgaa tttaaagact cactctccat aaatgctacg aatattaaac
     6541 acttcaaaaa ctgcacctcc atcagtggcg atctccacat cctgccggtg gcatttaggg
     6601 gtgactcctt cacacatact cctcctctgg atccacagga actggatatt ctgaaaaccg
     6661 taaaggaaat cacagggttt ttgctgattc aggcttggcc tgaaaacagg acggacctcc
     6721 atgcctttga gaacctagaa atcatacgcg gcaggaccaa gcaacatggt cagttttctc
     6781 ttgcagtcgt cagcctgaac ataacatcct tgggattacg ctccctcaag gagataagtg
     6841 atggagatgt gataatttca ggaaacaaaa atttgtgcta tgcaaataca ataaactgga
     6901 aaaaactgtt tgggacctcc ggtcagaaaa ccaaaattat aagcaacaga ggtgaaaaca
     6961 gctgcaaggc cacaggccag gtctgccatg ccttgtgctc ccccgagggc tgctggggcc
     7021 cggagcccag ggactgcgtc tcttgccgga atgtcagccg aggcagggaa tgcgtggaca
     7081 agtgcaacct tctggagggt gagccaaggg agtttgtgga gaactctgag tgcatacagt
     7141 gccacccaga gtgcctgcct caggccatga acatcacctg cacaggacgg ggaccagaca
     7201 actgtatcca gtgtgcccac tacattgacg gcccccactg cgtcaagacc tgcccggcag
     7261 gagtcatggg agaaaacaac accctggtct ggaagtacgc agacgccggc catgtgtgcc
     7321 acctgtgcca tccaaactgc acctacggat gcactgggcc aggtcttgaa ggctgtccaa
     7381 cgaatgggcc taagatcccg tccatcgcca ctgggatggt gggggccctc ctcttgctgc
     7441 tggtggtggc cctggggatc ggcctcttca tgcgaaggcg ccacatcgtt cggaagcgca
     7501 cgctgcggag gctgctgcag gagagggagc ttgtggagcc tcttacaccc agtggagaag
     7561 ctcccaacca agctctcttg aggatcttga aggaaactga attcaaaaag atcaaagtgc
     7621 tgggctccgg tgcgttcggc acggtgtata agggactctg gatcccagaa ggtgagaaag
     7681 ttaaaattcc cgtcgctatc aaggaattaa gagaagcaac atctccgaaa gccaacaagg
     7741 aaatcctcga tgaagcctac gtgatggcca gcgtggacaa cccccacgtg tgccgcctgc
     7801 tgggcatctg cctcacctcc accgtgcagc tcatcacgca gctcatgccc ttcggctgcc
     7861 tcctggacta tgtccgggaa cacaaagaca atattggctc ccagtacctg ctcaactggt
     7921 gtgtgcagat cgcaaagggc atgaactact tggaggaccg tcgcttggtg caccgcgacc
     7981 tggcagccag gaacgtactg gtgaaaacac cgcagcatgt caagatcaca gattttgggc
     8041 tggccaaact gctgggtgcg gaagagaaag aataccatgc agaaggaggc aaagtgccta
     8101 tcaagtggat ggcattggaa tcaattttac acagaatcta tacccaccag agtgatgtct
     8161 ggagctacgg ggtgaccgtt tgggagttga tgacctttgg atccaagcca tatgacggaa
     8221 tccctgccag cgagatctcc tccatcctgg agaaaggaga acgcctccct cagccaccca
     8281 tatgtaccat cgatgtctac atgatcatgg tcaagtgctg gatgatagac gcagatagtc
     8341 gcccaaagtt ccgtgagttg atcatcgaat tctccaaaat ggcccgagac ccccagcgct
     8401 accttgtcat tcagggggat gaaagaatgc atttgccaag tcctacagac tccaacttct
     8461 accgtgccnt gatggatgaa gaagacatgg acgacgtggt ggatgccgac gagtacctca
     8521 tcccacagca gggcttcttc agcagcccct ccacgtcacg gactcccctc ctgagctctc
     8581 tgagtgcaac cagcaacaat tccaccgtgg cttgcattga tagaaatggg ctgcaaagct
     8641 gtcccatcaa ggaagacagc ttcttgcagc gatacagctc agaccccaca ggcgccttga
     8701 ctgaggacag catagacgac accttcctcc cagtgcctga atacataaac cagtccgttc
     8761 ccaaaaggcc cgctggctct gtgcagaatc ctgtctatca caatcagcct ctgaaccccg
     8821 cgcccagcag agacccacac taccaggacc cccacagcac tgcagtgggc aaccccgagt
     8881 atctcaacac tgtccagccc acctgtgtca acagcacatt cgacagccct gcccactggg
     8941 cccagaaagg cagccaccaa attagcctgg acaaccctga ctaccagcag gacttctttc
     9001 ccaaggaagc caagccaaat ggcatcttta agggctccac agctgaaaat gcagaatacc
     9061 taagggtcgc gccacaaagc agtgaattta ttggagcaag cggccgcgct ttgcctggca
     9121 atcccgatca cagggaaatg ggggaaactc tcccagaaga ggttggagaa tatcggcagc
     9181 catctggtgg cagtgtacct gtgtctccgg gtccgcccag tggtcttgaa cccactagct
     9241 cctcacctta cggcggcggc tcctttaaca gctcaatcaa caacatccac gaaatggaga
     9301 ttcagctgaa ggatgcactc gaaaagaatc aacaatggct cgtttacgac caacaacgcg
     9361 aggtgtacgt gaaaggattg cttgcaaaaa ttttcgaact ggaaaagaaa acagagactg
     9421 cagcacatag tcttccttga c
//
