LOCUS       good_Copy_of_2023_07_27 6519 bp ds-DNA     circular     25-SEP-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    380..402
                     /label="seq_aav_itr"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     repeat_region   651..791
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     repeat_region   662..791
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(861..1568)
                     /label="mtagBFP2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1578..1585
                     /label="Kozak"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     misc_feature    1590..2390
                     /label="a1fe-mep-f"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(1595..1603)
                     /label="post"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        complement(1595..1834)
                     /label="EF-1-alpha promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(1604..1666)
                     /label="directional transcription start"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(1636..1636)
                     /label="transcription start"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(1660..1666)
                     /label="TATA box"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(1686..1705)
                     /label="EFP2"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(1716..1735)
                     /label="EPF1"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1821..1855
                     /label="ef1a-bd-r"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1835..1859
                     /label="seq-ef1aBD"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1840..1884
                     /label="cmve-f"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     enhancer        1860..2163
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2110..2131
                     /label="scrPCR-F bidirectional SplRepGFP-MS2-BFP 3888-3909"
                     /note="sequence: tgggactttcctacttggcagt"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2139..2179
                     /label="cmve-r"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2164..2181
                     /label="mep-f2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2164..2389
                     /label="meP229"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2247..2294
                     /label="apparently gone"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2291..2297
                     /label="gggcggg (bidirectional transcription)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2334..2337
                     /label="transcriptonstart"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2388..2389
                     /label="extra 2 c's for kozak"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2391..2391
                     /label="core-cit-f2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2391..2391
                     /label="ef1abd-cit-r"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2391..2391
                     /label="bgh-core-r"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2431..2440
                     /label="Ward Kozak"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             2441..2570
                     /label="hi"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MGIWWTVTNFGEISGTIAIEMDKGTYIHALDNGLFTLGAPHKE"
     primer          2445..2476
                     /label="BstBI_FRG1_gDNA_ex3_FWD"
                     /note="sequence: agctagcgaattcgaatttaaatcggatctGGCTAGCACCATGGAATCTGGTGGACAGTAACAAACTTTGGTGAA"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2445..2570
                     /label="Exon3"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2553..2634
                     /label="replace"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2592..2594
                     /label="STOP Codon"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2595..2613
                     /label="MS2 C High Affinity"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3373..3430
                     /label="Exon4"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(3398..3430)
                     /label="AscI_FRG1_gDNA_ex4_REV"
                     /note="sequence: ttttgccctgggcgcgccCTGGAATCAGATAATTTGACAGCCGTAAACTGC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3431..3439
                     /label="Functional\purpose??"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3440..3535
                     /label="factor2a"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3536..4255
                     /label="egfp"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    4253..4255
                     /label="Stop\Codon"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4258..4266
                     /label="core-cit-r"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    4262..4323
                     /label="nhei-sv40-f2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    4285..4415
                     /label="SV40 pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     misc_feature    4385..4433
                     /label="sv40-rsrii-r"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     repeat_region   4432..4561
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     repeat_region   4432..4572
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     rep_origin      4647..5102
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        5384..5488
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             5489..5557
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             5558..6349
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt cctgcaggca
      661 gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt
      721 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
      781 taggggttcc tgcggccgca CGCGTAATAA AAGATCTTTA TTTTCATTAG ATCTGTGTGT
      841 TGGTTTTTTG TGTGctagct attaagcttg tgccccagtt tgctagggag gtcgcagtat
      901 ctggccactg ccacctcgtg ctgctcgacg taggtttcgt tgttggcctc cttgattctt
      961 tccagtctgt agtccacata gtagacgcca ggcatcttga ggttcttagc gggtttcttg
     1021 gatctatatg tggtcttggc gtttgcgatc agatggctcc cgcccacgag cttcagggcc
     1081 atgtcgtttc tgccttccag gccgccgtca gcggggtaca gcgtttcggt gaaggcctcc
     1141 cagccgagtg ttttcttctg catcacaggg ccgttggatg tgaagttcac ccctctgatc
     1201 ttgacgttgt agatgaggca gccgtcctgg aggctggtgt cctgggtagc ggtcagcacg
     1261 cccccgtcct cgtatgtggt gactctctcc catgtgaagc cctcagggaa ggactgcttg
     1321 aagaagtcgg ggatgccctg ggtgtggttg atgaaggtct tgctgccgta gaggaagcta
     1381 gtagccagga tgtcgaaggc gaaggggaga gggccgccct cgaccacctt gattctcatg
     1441 gtctgggtgc cctcgtaggg cttgccttcg ccctcggatg tgcacttgaa gtgatggttg
     1501 tccacggtgc cctccatgta cagcttcatg tgcatgttct ccttaatcag ctcttcgccc
     1561 ttagacacgg atcccatggt ggcgaagcGt cgactcacga cacctgtgtt ctggcggcaa
     1621 acccgttgcg aaaaagaacg ttcacggcga ctactgcact tatatacggt tctcccccac
     1681 cctcgggaaa aaggcggagc cagtacacga catcactttc ccagtttacc ccgcgccacc
     1741 ttctctaggc accggttcaa ttgccgaccc ctccccccaa cttctcgggg actgtgggcg
     1801 atgtgcgctc tgcccactga cgggcaccgg agccCTATGA CTTGCATAGC TGCGTATGGc
     1861 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     1921 acgtcaATAA TGACGTATGT TCCCatagta acgccaatag ggactttcca ttgacgtcaa
     1981 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     2041 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     2101 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     2161 atgAATTGAG GGCGTCACCG CTAAGGCTCC GCCCCAGCCT GGGCTCCACA ACCAATGAAG
     2221 GGTAATCTCG ACAAAGAGCA AGGGGTGGGG CGCGGGCGCG CAGGTGCAGC AGCACACAGG
     2281 CTGgtcggga gggcggggcg cgacgtctgc cgtgcggggt cccggcatcg gttgcgcgcg
     2341 cgctccctcc tctcggagag agggctgtgg taaaacccgt ccggaaaCCa cTTAAGctag
     2401 agctagcgaa ttcgaattta aatcggatct GGCTAGCACC ATGGGAATCT GGTGGACAGT
     2461 AACAAACTTT GGTGAAATTT CAGGAACCAT AGCCATTGAA ATGGATAAGG GAACCTATAT
     2521 ACATGCACTC GACAATGGTC TTTTTACCCT GGGAGCTCCA CACAAAGAAG GTTTGTGTCT
     2581 GGAAGGGAAG ATAGctgcgt cctggtggcg cagGGCTGCC ACAAGTGTAG ATTTTAGGAC
     2641 ATTCATTCAC TTAGGCCAAA CTCTAACTAG TCTCAAACAT TTTCCAAAGG AATGGAACCA
     2701 TTGCATTTGA CTCTTCCATT TTTTTTTAAT TCCTTAATAT TTACCAGCCA TTGGCAAGTC
     2761 CCTCTTTCTA ATATAAGCAT TTGAAAACAT TCCGTATAGT TCCAGAAGAG TATCTTTGGA
     2821 AATCAAAATA ACATGAATAA TTAAAATAGT AAGTGGAAAG AAAAAAGAAA ACCTATTTGA
     2881 GTCAAAATGT TTGAATTTCT TTTTTATTCA GACCTATTGC CAAAACTAAC CTGGGTGCGT
     2941 TTAACAGTTT GAAGTTTCCT CATTTTCTTT AGCCCATTAG AGGAAGCACT AATGAGATAT
     3001 GAAGTAATTA GAAGATAAAA AGCAGTATCA TTTGGTCAGC AAGGCCATCC CTTGAATAAG
     3061 TGAAAGCATT TAGCTTTTTA AATAAGTGCT TATTTATGAC TGTATTTTAA AACATAAAGA
     3121 GAAGCTATCT CAAAAGTTAT TAAATAAGCA TTATATCACC CTGTCTTACT CAGTGTATAA
     3181 AATTAACACT GTAGTTAAAA GAAGGTAAAA TAGATCTTGA TTCCAAAGAT ACAGTATTAC
     3241 AGTGACATCA GTATATCTGA ATATTCTTAC ATTTAATTGC AGAAGAAAAT AGCCACATTT
     3301 TTTAAAACAA AATAATGTCT TTATATTTAT AGCAAATGTC AAATTTATTT TCAAATGTTT
     3361 TTTCTCCAAT AGTTGATGAG GGCCCTAGTC CTCCAGAGCA GTTTACGGCT GTCAAATTAT
     3421 CTGATTCCAG ggcgcgccca gggcaaaacg ttcgggttcg ggtgcgccag taaagcagac
     3481 attaaacttt gatttgctga aacttgcagg tgatgtagag tcaaatccag gtccaatggt
     3541 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     3601 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     3661 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     3721 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     3781 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     3841 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     3901 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     3961 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     4021 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     4081 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     4141 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     4201 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaATcga
     4261 taccgagcgc tgctcgaggc tagcaacttg tttattgcag cttataatgg ttacaaataa
     4321 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     4381 ttgtccaaac tcatcaatgt atcttatcat gtctgcggac cgagcggccg caggaacccc
     4441 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     4501 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     4561 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     4621 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     4681 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     4741 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4801 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4861 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4921 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4981 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5041 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     5101 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     5161 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     5221 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     5281 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     5341 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5401 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5461 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5521 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5581 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5641 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     5701 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     5761 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5821 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5881 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5941 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6001 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6061 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6121 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6181 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6241 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6301 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6361 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6421 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6481 ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//