LOCUS       VN0516-pBRalpha-IP      5188 bp    DNA     circular UNA UNA
FEATURES             Location/Qualifiers
     Region          124..148
                     /note="Match: CGAACGATCAAAAATAAGTGCCTTC"
                     /note="Length of match: 25"
                     /note="Accuracy (%): 100"
                     /note="Description: pBRa_S"
                     /note="Found at strand: Positive"
                     /label=OR329
     Region          complement(124..148)
                     /note="Match: GAAGGCACTTATTTTTGATCGTTCG"
                     /note="Length of match: 25"
                     /note="Accuracy (%): 100"
                     /note="Description: VN208_Dplpp_AS"
                     /note="Found at strand: Negative"
                     /label=OR429
     -35_signal      163..168
                     /label="-35 (plpp)"
                     /label=""-35 (plpp)""
     -10_signal      188..193
                     /label="-10 (plpp)"
                     /label=""-10 (plpp)""
     -35_signal      235..240
                     /label="placUV5 -35"
                     /label=""placUV5 -35""
     -10_signal      259..264
                     /label="-10 (placUV5)"
                     /label=""-10 (placUV5)""
     Site            271..292
                     /label=lacO
     misc_signal     271
                     /label="+1 (placUV5)"
                     /label=""+1 (placUV5)""
     Region          273..289
                     /note="Match: ttgtgagcggataacaa"
                     /note="Length of match: 17"
                     /note="Accuracy (%): 100"
                     /note="Description: pLac"
                     /note="Found at strand: Positive"
                     /label=OR16
     Region          complement(275..297)
                     /note="Match: gtgtgaaattgttatccgctcac"
                     /note="Length of match: 23"
                     /note="Accuracy (%): 100"
                     /note="Description: ptrc_dRBS_AS"
                     /note="Found at strand: Negative"
                     /label=OR394
     Region          277..300
                     /note="Match: gagcggataacaatttcacacagg"
                     /note="Length of match: 24"
                     /note="Accuracy (%): 100"
                     /note="Description: M13_rev_-49"
                     /note="Found at strand: Positive"
                     /label=OR60
     Region          297..314
                     /note="Match: caggaaacagctatgacc"
                     /note="Length of match: 18"
                     /note="Accuracy (%): 100"
                     /note="Description: M13_rev_-29"
                     /note="Found at strand: Positive"
                     /label=OR61
     Region          332..354
                     /note="Match: ggaactctagaccaaagagagga"
                     /note="Length of match: 23"
                     /note="Accuracy (%): 100"
                     /note="Description: XbaI_RBS_S"
                     /note="Found at strand: Positive"
                     /label=OR306
     RBS             351..354
     Region          359..1102
                     /label=NTD-rpoA
     CDS             359..1201
                     /label=cI
                     /label=rpoA-IP2
     Region          complement(362..381)
                     /note="Match: aactctgtcacagaaccctg"
                     /note="Length of match: 20"
                     /note="Accuracy (%): 100"
                     /note="Description: pLTetO_star_AS"
                     /note="Found at strand: Negative"
                     /label=OR427
     Region          362..382
                     /note="Match: cagggttctgtgacagagttt"
                     /note="Length of match: 21"
                     /note="Accuracy (%): 100"
                     /note="Description: VN208_Dplpp_S"
                     /note="Found at strand: Positive"
                     /label=OR428
     Region          708..726
                     /note="Match: acgacggtgatgtcgaaat"
                     /note="Length of match: 19"
                     /note="Accuracy (%): 100"
                     /note="Description: RNApol_S1"
                     /note="Found at strand: Positive"
                     /label=OR139
     Region          935..955
                     /note="Match: gaacagcgtaccgacctggac"
                     /note="Length of match: 21"
                     /note="Accuracy (%): 100"
                     /note="Description: pBRa_F"
                     /note="Found at strand: Positive"
                     /label=OR234
     Region          complement(988..1006)
                     /note="Match: cgcctcttcaggatcgatt"
                     /note="Length of match: 19"
                     /note="Accuracy (%): 100"
                     /note="Description: RNApol_AS1"
                     /note="Found at strand: Negative"
                     /label=OR140
     Region          988..1006
                     /note="Match: aatcgatcctgaagaggcg"
                     /note="Length of match: 19"
                     /note="Accuracy (%): 100"
                     /note="Description: RNApol_S2"
                     /note="Found at strand: Positive"
                     /label=OR143
     Site            join(1103..1110,1104^1105)
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 1104^1105"
                     /note="cut_0_on_negative_strand: 1108^1109"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
     Region          1105..1202
                     /note="Match: GGCCGCAACCAAAAACTACAAACAGACCAGCGTTTGAG"
                     /note="Length of match: 38"
                     /note="Accuracy (%): 100"
                     /note="Description: NotI_CRIPT_S"
                     /note="Found at strand: Positive"
                     /label=OR228
     Region          1112..1198
                     /label=IP2
     Region          complement(1179..1197)
                     /note="Match: ACGCTGGTCTGTTTGTAGT"
                     /note="Length of match: 19"
                     /note="Accuracy (%): 100"
                     /note="Description: CRIPT_Sb"
                     /note="Found at strand: Negative"
                     /label=OR177
     misc_signal     1199..1201
                     /label=Stop
     Site            join(1202..1207,1202^1203)
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="cut_0_on_positive_strand: 1202^1203"
                     /note="cut_0_on_negative_strand: 1206^1207"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     primer          complement(1420..1440)
                     /label=pBRa_AS(OR235)
     Site            join(1478..1483,1478^1479)
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1391^1392"
                     /note="cut_0_on_negative_strand: 1395^1396"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Region          complement(2348..2367)
                     /note="Match: cgcgtatcggtgattcattc"
                     /note="Length of match: 20"
                     /note="Accuracy (%): 100"
                     /note="Description: DtetR_Seq_AS"
                     /note="Found at strand: Negative"
                     /label=OR118
     Region          2500..2534
                     /note="Match: gcatcgcaggatgctgctggctaccctgtggaaca"
                     /note="Length of match: 35"
                     /note="Accuracy (%): 100"
                     /note="Description: VN278FRAG1_S"
                     /note="Found at strand: Positive"
                     /label=OR336
     CDS             2742..2933
                     /label=rop
     Region          2983..3003
                     /note="Match: ggagcagacaagcccgtcagg"
                     /note="Length of match: 21"
                     /note="Accuracy (%): 100"
                     /note="Description: pEX-for"
                     /note="Found at strand: Positive"
                     /label=OR172
     Region          3055..3075
                     /note="Match: gtagcgatagcggagtgtata"
                     /note="Length of match: 21"
                     /note="Accuracy (%): 100"
                     /note="Description: LacZ_Seq_S"
                     /note="Found at strand: Positive"
                     /label=OR119
     Region          complement(3241..3257)
                     /note="Match: ccgcctttgagtgagct"
                     /note="Length of match: 17"
                     /note="Accuracy (%): 100"
                     /note="Description: LacZ_Seq_AS"
                     /note="Found at strand: Negative"
                     /label=OR120
     rep_origin      complement(3361..3949)
                     /label=pBR322
     Region          complement(3656..3677)
                     /note="Match: tggactcaagacgatagttacc"
                     /note="Length of match: 22"
                     /note="Accuracy (%): 100"
                     /note="Description: Ori"
                     /note="Found at strand: Negative"
                     /label=OR187
     CDS             complement(4120..4980)
                     /label=AmpR
     Region          4396..4416
                     /note="Match: ttcgccagttaatagtttgcg"
                     /note="Length of match: 21"
                     /note="Accuracy (%): 100"
                     /note="Description: AmpR_AS"
                     /note="Found at strand: Positive"
                     /label=OR185
     Region          complement(4461..4481)
                     /note="Match: tgaatgaagccataccaaacg"
                     /note="Length of match: 21"
                     /note="Accuracy (%): 100"
                     /note="Description: AmpR_S"
                     /note="Found at strand: Negative"
                     /label=OR186
     Region          5109..5135
                     /note="Match: ctgacgtctaagaaaccattattatca"
                     /note="Length of match: 27"
                     /note="Accuracy (%): 100"
                     /note="Description: DtetR_Seq_S"
                     /note="Found at strand: Positive"
                     /label=OR117
ORIGIN
        1 GCAATAAAAA ATCAAATCGG ATTTCACTAT ATAATCTCAC TTTATCTAAG ATGAATCCGA
       61 TGGAAGCATC CTGTTTTCTC TCAATTTTTT TATCTAAAAC CCAGCGTTCG ATGCTTCTTT
      121 GAGCGAACGA TCAAAAATAA GTGCCTTCCC ATCAAAAAAA TATTGACAAC ATAAAAAACT
      181 TTGTGTTATA CTTGTAACGC TACATGGAGA TTAACTCAAT CTAGCTAGAG AGGCTTTACA
      241 CTTTATGCTT CCGGCTCGTA TAATGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG
      301 AAACAGCTAT GACCATGATT ACGGATTCAC TGGAACTCTA GACCAAAGAG AGGACACAAT
      361 GCAGGGTTCT GTGACAGAGT TTCTAAAACC GCGCCTGGTT GATATCGAGC AAGTGAGTTC
      421 GACGCACGCC AAGGTGACCC TTGAGCCTTT AGAGCGTGGC TTTGGCCATA CTCTGGGTAA
      481 CGCACTGCGC CGTATTCTGC TCTCATCGAT GCCGGGTTGC GCGGTGACCG AGGTTGAGAT
      541 TGATGGTGTA CTACATGAGT ACAGCACCAA AGAAGGCGTT CAGGAAGATA TCCTGGAAAT
      601 CCTGCTCAAC CTGAAAGGGC TGGCGGTGAG AGTTCAGGGC AAAGATGAAG TTATTCTTAC
      661 CTTGAATAAA TCTGGCATTG GCCCTGTGAC TGCAGCCGAT ATCACCCACG ACGGTGATGT
      721 CGAAATCGTC AAGCCGCAGC ACGTGATCTG CCACCTGACC GATGAGAACG CGTCTATTAG
      781 CATGCGTATC AAAGTTCAGC GCGGTCGTGG TTATGTGCCG GCTTCTACCC GAATTCATTC
      841 GGAAGAAGAT GAGCGCCCAA TCGGCCGTCT GCTGGTCGAC GCATGCTACA GCCCTGTGGA
      901 GCGTATTGCC TACAATGTTG AAGCAGCGCG TGTAGAACAG CGTACCGACC TGGACAAGCT
      961 GGTCATCGAA ATGGAAACCA ACGGCACAAT CGATCCTGAA GAGGCGATTC GTCGTGCGGC
     1021 AACCATTCTG GCTGAACAAC TGGAAGCTTT CGTTGACTTA CGTGATGTAC GTCAGCCTGA
     1081 AGTGAAAGAA GAGAAACCAG AGGCGGCCGC AGGCGCGATG GGCACCATCA TGCCGAAAGA
     1141 TATCCAGCTG GCGCGTCGTA TCCGTGGCGC GGGCGGCCGT ACCCTGTACG GCTTTGGCTG
     1201 AGGATCCTCT ACGCCGGACG CATCGTGGCC GGCATCACCG GCGCCACAGG TGCGGTTGCT
     1261 GGCGCCTATA TCGCCGACAT CACCGATGGG GAAGATCGGG CTCGCCACTT CGGGCTCATG
     1321 AGCGCTTGTT TCGGCGTGGG TATGGTGGCA GGCCCCGTGG CCGGGGGACT GTTGGGCGCC
     1381 ATCTCCTTGC ATGCACCATT CCTTGCGGCG GCGGTGCTCA ACGGCCTCAA CCTACTACTG
     1441 GGCTGCTTCC TAATGCAGGA GTCGCATAAG GGAGAGCGTC GACCGATGCC CTTGAGAGCC
     1501 TTCAACCCAG TCAGCTCCTT CCGGTGGGCG CGGGGCATGA CTATCGTCGC CGCACTTATG
     1561 ACTGTCTTCT TTATCATGCA ACTCGTAGGA CAGGTGCCGG CAGCGCTCTG GGTCATTTTC
     1621 GGCGAGGACC GCTTTCGCTG GAGCGCGACG ATGATCGGCC TGTCGCTTGC GGTATTCGGA
     1681 ATCTTGCACG CCCTCGCTCA AGCCTTCGTC ACTGGTCCCG CCACCAAACG TTTCGGCGAG
     1741 AAGCAGGCCA TTATCGCCGG CATGGCGGCC GACGCGCTGG GCTACGTCTT GCTGGCGTTC
     1801 GCGACGCGAG GCTGGATGGC CTTCCCCATT ATGATTCTTC TCGCTTCCGG CGGCATCGGG
     1861 ATGCCCGCGT TGCAGGCCAT GCTGTCCAGG CAGGTAGATG ACGACCATCA GGGACAGCTT
     1921 CAAGGATCGC TCGCGGCTCT TACCAGCCTA ACTTCGATCA CTGGACCGCT GATCGTCACG
     1981 GCGATTTATG CCGCCTCGGC GAGCACATGG AACGGGTTGG CATGGATTGT AGGCGCCGCC
     2041 CTATACCTTG TCTGCCTCCC CGCGTTGCGT CGCGGTGCAT GGAGCCGGGC CACCTCGACC
     2101 TGAATGGAAG CCGGCGGCAC CTCGCTAACG GATTCACCAC TCCAAGAATT GGAGCCAATC
     2161 AATTCTTGCG GAGAACTGTG AATGCGCAAA CCAACCCTTG GCAGAACATA TCCATCGCGT
     2221 CCGCCATCTC CAGCAGCCGC ACGCGGCGCA TCTCGGGCAG CGTTGGGTCC TGGCCACGGG
     2281 TGCGCATGAT CGTGCTCCTG TCGTTGAGGA CCCGGCTAGG CTGGCGGGGT TGCCTTACTG
     2341 GTTAGCAGAA TGAATCACCG ATACGCGAGC GAACGTGAAG CGACTGCTGC TGCAAAACGT
     2401 CTGCGACCTG AGCAACAACA TGAATGGTCT TCGGTTTCCG TGTTTCGTAA AGTCTGGAAA
     2461 CGCGGAAGTC AGCGCCCTGC ACCATTATGT TCCGGATCTG CATCGCAGGA TGCTGCTGGC
     2521 TACCCTGTGG AACACCTACA TCTGTATTAA CGAAGCGCTG GCATTGACCC TGAGTGATTT
     2581 TTCTCTGGTC CCGCCGCATC CATACCGCCA GTTGTTTACC CTCACAACGT TCCAGTAACC
     2641 GGGCATGTTC ATCATCAGTA ACCCGTATCG TGAGCATCCT CTCTCGTTTC ATCGGTATCA
     2701 TTACCCCCAT GAACAGAAAT CCCCCTTACA CGGAGGCATC AGTGACCAAA CAGGAAAAAA
     2761 CCGCCCTTAA CATGGCCCGC TTTATCAGAA GCCAGACATT AACGCTTCTG GAGAAACTCA
     2821 ACGAGCTGGA CGCGGATGAA CAGGCAGACA TCTGTGAATC GCTTCACGAC CACGCTGATG
     2881 AGCTTTACCG CAGCTGCCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC
     2941 AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC
     3001 AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCGCAGC CATGACCCAG TCACGTAGCG
     3061 ATAGCGGAGT GTATACTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA
     3121 CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCTC
     3181 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC
     3241 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA
     3301 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT
     3361 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     3421 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     3481 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     3541 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     3601 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     3661 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     3721 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     3781 TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     3841 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     3901 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
     3961 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
     4021 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
     4081 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA
     4141 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT
     4201 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG
     4261 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA
     4321 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA
     4381 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTGCAGG
     4441 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC
     4501 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC
     4561 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA
     4621 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC
     4681 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAACACG
     4741 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC
     4801 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG
     4861 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC
     4921 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT
     4981 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
     5041 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA
     5101 AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG
     5161 TATCACGAGG CCCTTTGGAT AACCAGAA
//
