LOCUS       VN0518-pBRalpha-IP      5179 bp    DNA     circular UNA UNA
FEATURES             Location/Qualifiers
     Region          124..148
                     /note="Match: CGAACGATCAAAAATAAGTGCCTTC"
                     /note="Length of match: 25"
                     /note="Accuracy (%): 100"
                     /note="Description: pBRa_S"
                     /note="Found at strand: Positive"
                     /label=OR329
     Region          complement(124..148)
                     /note="Match: GAAGGCACTTATTTTTGATCGTTCG"
                     /note="Length of match: 25"
                     /note="Accuracy (%): 100"
                     /note="Description: VN208_Dplpp_AS"
                     /note="Found at strand: Negative"
                     /label=OR429
     -35_signal      163..168
                     /label="-35 (plpp)"
                     /label=""-35 (plpp)""
     -10_signal      188..193
                     /label="-10 (plpp)"
                     /label=""-10 (plpp)""
     -35_signal      235..240
                     /label="placUV5 -35"
                     /label=""placUV5 -35""
     -10_signal      259..264
                     /label="-10 (placUV5)"
                     /label=""-10 (placUV5)""
     Site            271..292
                     /label=lacO
     misc_signal     271
                     /label="+1 (placUV5)"
                     /label=""+1 (placUV5)""
     Region          273..289
                     /note="Match: ttgtgagcggataacaa"
                     /note="Length of match: 17"
                     /note="Accuracy (%): 100"
                     /note="Description: pLac"
                     /note="Found at strand: Positive"
                     /label=OR16
     Region          complement(275..297)
                     /note="Match: gtgtgaaattgttatccgctcac"
                     /note="Length of match: 23"
                     /note="Accuracy (%): 100"
                     /note="Description: ptrc_dRBS_AS"
                     /note="Found at strand: Negative"
                     /label=OR394
     Region          277..300
                     /note="Match: gagcggataacaatttcacacagg"
                     /note="Length of match: 24"
                     /note="Accuracy (%): 100"
                     /note="Description: M13_rev_-49"
                     /note="Found at strand: Positive"
                     /label=OR60
     Region          297..314
                     /note="Match: caggaaacagctatgacc"
                     /note="Length of match: 18"
                     /note="Accuracy (%): 100"
                     /note="Description: M13_rev_-29"
                     /note="Found at strand: Positive"
                     /label=OR61
     Region          332..354
                     /note="Match: ggaactctagaccaaagagagga"
                     /note="Length of match: 23"
                     /note="Accuracy (%): 100"
                     /note="Description: XbaI_RBS_S"
                     /note="Found at strand: Positive"
                     /label=OR306
     RBS             351..354
     Region          359..1102
                     /label=NTD-rpoA
     CDS             359..1192
                     /label=cI
                     /label=rpoA-IP4
     Region          complement(362..381)
                     /note="Match: aactctgtcacagaaccctg"
                     /note="Length of match: 20"
                     /note="Accuracy (%): 100"
                     /note="Description: pLTetO_star_AS"
                     /note="Found at strand: Negative"
                     /label=OR427
     Region          362..382
                     /note="Match: cagggttctgtgacagagttt"
                     /note="Length of match: 21"
                     /note="Accuracy (%): 100"
                     /note="Description: VN208_Dplpp_S"
                     /note="Found at strand: Positive"
                     /label=OR428
     Region          708..726
                     /note="Match: acgacggtgatgtcgaaat"
                     /note="Length of match: 19"
                     /note="Accuracy (%): 100"
                     /note="Description: RNApol_S1"
                     /note="Found at strand: Positive"
                     /label=OR139
     Region          935..955
                     /note="Match: gaacagcgtaccgacctggac"
                     /note="Length of match: 21"
                     /note="Accuracy (%): 100"
                     /note="Description: pBRa_F"
                     /note="Found at strand: Positive"
                     /label=OR234
     Region          complement(988..1006)
                     /note="Match: cgcctcttcaggatcgatt"
                     /note="Length of match: 19"
                     /note="Accuracy (%): 100"
                     /note="Description: RNApol_AS1"
                     /note="Found at strand: Negative"
                     /label=OR140
     Region          988..1006
                     /note="Match: aatcgatcctgaagaggcg"
                     /note="Length of match: 19"
                     /note="Accuracy (%): 100"
                     /note="Description: RNApol_S2"
                     /note="Found at strand: Positive"
                     /label=OR143
     Site            join(1044..1049,1044^1045)
                     /note="Name: HindIII"
                     /note="Pattern: aagctt"
                     /note="cut_0_on_positive_strand: 1044^1045"
                     /note="cut_0_on_negative_strand: 1048^1049"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=HindIII
     Site            join(1103..1110,1104^1105)
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 1104^1105"
                     /note="cut_0_on_negative_strand: 1108^1109"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
     Region          1105..1193
                     /note="Match: GGCCGCAACCAAAAACTACAAACAGACCAGCGTTTGAG"
                     /note="Length of match: 38"
                     /note="Accuracy (%): 100"
                     /note="Description: NotI_CRIPT_S"
                     /note="Found at strand: Positive"
                     /label=OR228
     Region          1112..1189
                     /label=IP4
     Region          complement(1170..1188)
                     /note="Match: ACGCTGGTCTGTTTGTAGT"
                     /note="Length of match: 19"
                     /note="Accuracy (%): 100"
                     /note="Description: CRIPT_Sb"
                     /note="Found at strand: Negative"
                     /label=OR177
     misc_signal     1190..1192
                     /label=Stop
     Site            join(1193..1198,1193^1194)
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="cut_0_on_positive_strand: 1193^1194"
                     /note="cut_0_on_negative_strand: 1197^1198"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     Region          complement(1411..1431)
                     /note="Match: cagtagtaggttgaggccgtt"
                     /note="Length of match: 21"
                     /note="Accuracy (%): 100"
                     /note="Description: pBRa_AS"
                     /note="Found at strand: Negative"
                     /label=OR235
     Site            join(1469..1474,1469^1470)
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1391^1392"
                     /note="cut_0_on_negative_strand: 1395^1396"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Region          complement(2339..2358)
                     /note="Match: cgcgtatcggtgattcattc"
                     /note="Length of match: 20"
                     /note="Accuracy (%): 100"
                     /note="Description: DtetR_Seq_AS"
                     /note="Found at strand: Negative"
                     /label=OR118
     Region          2491..2525
                     /note="Match: gcatcgcaggatgctgctggctaccctgtggaaca"
                     /note="Length of match: 35"
                     /note="Accuracy (%): 100"
                     /note="Description: VN278FRAG1_S"
                     /note="Found at strand: Positive"
                     /label=OR336
     CDS             2733..2924
                     /label=rop
     Region          2974..2994
                     /note="Match: ggagcagacaagcccgtcagg"
                     /note="Length of match: 21"
                     /note="Accuracy (%): 100"
                     /note="Description: pEX-for"
                     /note="Found at strand: Positive"
                     /label=OR172
     Region          3046..3066
                     /note="Match: gtagcgatagcggagtgtata"
                     /note="Length of match: 21"
                     /note="Accuracy (%): 100"
                     /note="Description: LacZ_Seq_S"
                     /note="Found at strand: Positive"
                     /label=OR119
     Region          complement(3232..3248)
                     /note="Match: ccgcctttgagtgagct"
                     /note="Length of match: 17"
                     /note="Accuracy (%): 100"
                     /note="Description: LacZ_Seq_AS"
                     /note="Found at strand: Negative"
                     /label=OR120
     rep_origin      complement(3352..3940)
                     /label=pBR322
     Region          complement(3647..3668)
                     /note="Match: tggactcaagacgatagttacc"
                     /note="Length of match: 22"
                     /note="Accuracy (%): 100"
                     /note="Description: Ori"
                     /note="Found at strand: Negative"
                     /label=OR187
     CDS             complement(4111..4971)
                     /label=AmpR
     Region          4387..4407
                     /note="Match: ttcgccagttaatagtttgcg"
                     /note="Length of match: 21"
                     /note="Accuracy (%): 100"
                     /note="Description: AmpR_AS"
                     /note="Found at strand: Positive"
                     /label=OR185
     Region          complement(4452..4472)
                     /note="Match: tgaatgaagccataccaaacg"
                     /note="Length of match: 21"
                     /note="Accuracy (%): 100"
                     /note="Description: AmpR_S"
                     /note="Found at strand: Negative"
                     /label=OR186
     Region          5100..5126
                     /note="Match: ctgacgtctaagaaaccattattatca"
                     /note="Length of match: 27"
                     /note="Accuracy (%): 100"
                     /note="Description: DtetR_Seq_S"
                     /note="Found at strand: Positive"
                     /label=OR117
ORIGIN
        1 GCAATAAAAA ATCAAATCGG ATTTCACTAT ATAATCTCAC TTTATCTAAG ATGAATCCGA
       61 TGGAAGCATC CTGTTTTCTC TCAATTTTTT TATCTAAAAC CCAGCGTTCG ATGCTTCTTT
      121 GAGCGAACGA TCAAAAATAA GTGCCTTCCC ATCAAAAAAA TATTGACAAC ATAAAAAACT
      181 TTGTGTTATA CTTGTAACGC TACATGGAGA TTAACTCAAT CTAGCTAGAG AGGCTTTACA
      241 CTTTATGCTT CCGGCTCGTA TAATGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG
      301 AAACAGCTAT GACCATGATT ACGGATTCAC TGGAACTCTA GACCAAAGAG AGGACACAAT
      361 GCAGGGTTCT GTGACAGAGT TTCTAAAACC GCGCCTGGTT GATATCGAGC AAGTGAGTTC
      421 GACGCACGCC AAGGTGACCC TTGAGCCTTT AGAGCGTGGC TTTGGCCATA CTCTGGGTAA
      481 CGCACTGCGC CGTATTCTGC TCTCATCGAT GCCGGGTTGC GCGGTGACCG AGGTTGAGAT
      541 TGATGGTGTA CTACATGAGT ACAGCACCAA AGAAGGCGTT CAGGAAGATA TCCTGGAAAT
      601 CCTGCTCAAC CTGAAAGGGC TGGCGGTGAG AGTTCAGGGC AAAGATGAAG TTATTCTTAC
      661 CTTGAATAAA TCTGGCATTG GCCCTGTGAC TGCAGCCGAT ATCACCCACG ACGGTGATGT
      721 CGAAATCGTC AAGCCGCAGC ACGTGATCTG CCACCTGACC GATGAGAACG CGTCTATTAG
      781 CATGCGTATC AAAGTTCAGC GCGGTCGTGG TTATGTGCCG GCTTCTACCC GAATTCATTC
      841 GGAAGAAGAT GAGCGCCCAA TCGGCCGTCT GCTGGTCGAC GCATGCTACA GCCCTGTGGA
      901 GCGTATTGCC TACAATGTTG AAGCAGCGCG TGTAGAACAG CGTACCGACC TGGACAAGCT
      961 GGTCATCGAA ATGGAAACCA ACGGCACAAT CGATCCTGAA GAGGCGATTC GTCGTGCGGC
     1021 AACCATTCTG GCTGAACAAC TGGAAGCTTT CGTTGACTTA CGTGATGTAC GTCAGCCTGA
     1081 AGTGAAAGAA GAGAAACCAG AGGCGGCCGC AGCAAGCACC GAAGAAAAAT GGGCACGTCT
     1141 GGCACGTCGT ATTGCAGGCG CAGGCGGTGT TACCCTGGAT GGTTTTGGTT GAGGATCCTC
     1201 TACGCCGGAC GCATCGTGGC CGGCATCACC GGCGCCACAG GTGCGGTTGC TGGCGCCTAT
     1261 ATCGCCGACA TCACCGATGG GGAAGATCGG GCTCGCCACT TCGGGCTCAT GAGCGCTTGT
     1321 TTCGGCGTGG GTATGGTGGC AGGCCCCGTG GCCGGGGGAC TGTTGGGCGC CATCTCCTTG
     1381 CATGCACCAT TCCTTGCGGC GGCGGTGCTC AACGGCCTCA ACCTACTACT GGGCTGCTTC
     1441 CTAATGCAGG AGTCGCATAA GGGAGAGCGT CGACCGATGC CCTTGAGAGC CTTCAACCCA
     1501 GTCAGCTCCT TCCGGTGGGC GCGGGGCATG ACTATCGTCG CCGCACTTAT GACTGTCTTC
     1561 TTTATCATGC AACTCGTAGG ACAGGTGCCG GCAGCGCTCT GGGTCATTTT CGGCGAGGAC
     1621 CGCTTTCGCT GGAGCGCGAC GATGATCGGC CTGTCGCTTG CGGTATTCGG AATCTTGCAC
     1681 GCCCTCGCTC AAGCCTTCGT CACTGGTCCC GCCACCAAAC GTTTCGGCGA GAAGCAGGCC
     1741 ATTATCGCCG GCATGGCGGC CGACGCGCTG GGCTACGTCT TGCTGGCGTT CGCGACGCGA
     1801 GGCTGGATGG CCTTCCCCAT TATGATTCTT CTCGCTTCCG GCGGCATCGG GATGCCCGCG
     1861 TTGCAGGCCA TGCTGTCCAG GCAGGTAGAT GACGACCATC AGGGACAGCT TCAAGGATCG
     1921 CTCGCGGCTC TTACCAGCCT AACTTCGATC ACTGGACCGC TGATCGTCAC GGCGATTTAT
     1981 GCCGCCTCGG CGAGCACATG GAACGGGTTG GCATGGATTG TAGGCGCCGC CCTATACCTT
     2041 GTCTGCCTCC CCGCGTTGCG TCGCGGTGCA TGGAGCCGGG CCACCTCGAC CTGAATGGAA
     2101 GCCGGCGGCA CCTCGCTAAC GGATTCACCA CTCCAAGAAT TGGAGCCAAT CAATTCTTGC
     2161 GGAGAACTGT GAATGCGCAA ACCAACCCTT GGCAGAACAT ATCCATCGCG TCCGCCATCT
     2221 CCAGCAGCCG CACGCGGCGC ATCTCGGGCA GCGTTGGGTC CTGGCCACGG GTGCGCATGA
     2281 TCGTGCTCCT GTCGTTGAGG ACCCGGCTAG GCTGGCGGGG TTGCCTTACT GGTTAGCAGA
     2341 ATGAATCACC GATACGCGAG CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT
     2401 GAGCAACAAC ATGAATGGTC TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT
     2461 CAGCGCCCTG CACCATTATG TTCCGGATCT GCATCGCAGG ATGCTGCTGG CTACCCTGTG
     2521 GAACACCTAC ATCTGTATTA ACGAAGCGCT GGCATTGACC CTGAGTGATT TTTCTCTGGT
     2581 CCCGCCGCAT CCATACCGCC AGTTGTTTAC CCTCACAACG TTCCAGTAAC CGGGCATGTT
     2641 CATCATCAGT AACCCGTATC GTGAGCATCC TCTCTCGTTT CATCGGTATC ATTACCCCCA
     2701 TGAACAGAAA TCCCCCTTAC ACGGAGGCAT CAGTGACCAA ACAGGAAAAA ACCGCCCTTA
     2761 ACATGGCCCG CTTTATCAGA AGCCAGACAT TAACGCTTCT GGAGAAACTC AACGAGCTGG
     2821 ACGCGGATGA ACAGGCAGAC ATCTGTGAAT CGCTTCACGA CCACGCTGAT GAGCTTTACC
     2881 GCAGCTGCCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG
     2941 AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT
     3001 CAGCGGGTGT TGGCGGGTGT CGGGGCGCAG CCATGACCCA GTCACGTAGC GATAGCGGAG
     3061 TGTATACTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG
     3121 GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCT CTTCCGCTTC
     3181 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC
     3241 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC
     3301 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG
     3361 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     3421 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     3481 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     3541 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     3601 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     3661 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     3721 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     3781 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     3841 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     3901 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
     3961 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT
     4021 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA
     4081 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT
     4141 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC
     4201 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG
     4261 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG
     4321 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT
     4381 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTGCAG GCATCGTGGT
     4441 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT
     4501 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT
     4561 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT
     4621 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT
     4681 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAACAC GGGATAATAC
     4741 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA
     4801 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA
     4861 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA
     4921 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT
     4981 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
     5041 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC
     5101 TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG
     5161 GCCCTTTGGA TAACCAGAA
//
