LOCUS       VN0550_pAC-cI_E34P      6156 bp    DNA     circular UNA 09-juil.-2013
DEFINITION  
FEATURES             Location/Qualifiers
     CDS             1..441
                     /label=CmR
     primer          672..694
                     /label=pAC-cI_Rev(OR237)
     Site            join(703^704,699..704)
                     /note="Name: BclI"
                     /note="Pattern: tgatca"
                     /note="cut_0_on_positive_strand: 3150^3151"
                     /note="cut_0_on_negative_strand: 3154^3155"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BclI
     primer          791..811
                     /label=OR757
     Site            join(804^805,800..805)
                     /note="Name: BglII"
                     /note="Pattern: agatct"
                     /note="cut_0_on_positive_strand: 3049^3050"
                     /note="cut_0_on_negative_strand: 3053^3054"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BglII
     Site            join(815..820,815^816)
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="cut_0_on_positive_strand: 815^816"
                     /note="cut_0_on_negative_strand: 819^820"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     misc_signal     complement(821..823)
                     /label=Stop
     CDS             complement(821..2011)
                     /label=cI-PDZ
                     /label=cI-Asf1
     misc_signal     complement(824..826)
                     /label=Stop
     Region          complement(827..1291)
                     /label=Asf1
     Site            join(1293..1300,1294^1295)
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 1294^1295"
                     /note="cut_0_on_negative_strand: 1298^1299"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
     Region          complement(1301..2011)
                     /label=lambda-cI-NTD
     primer          complement(1301..1319)
                     /label=OR756
     primer          complement(1408..1427)
                     /label=pAC-cI_F(OR236)
     primer          1408..1427
                     /label=ci-E34P-Seq(OR679)
     primer          1886..1920
                     /label=OR132
     primer          complement(1896..1919)
                     /label=PrVN529_AS(OR678)
     primer          1896..1919
                     /label=PrVN529_S(OR677)
     primer          complement(1896..1935)
                     /label=OR131
     mutation        complement(1907..1909)
                     /label=E34P
     RBS             complement(2020..2023)
     primer          complement(2021..2044)
                     /label=M13_rev_-49(OR60)
     Site            complement(2029..2050)
                     /label=lacO
     primer          complement(2032..2048)
                     /label=pLac(OR16)
     misc_signal     complement(2050)
                     /label=+1
     -10_signal      complement(2057..2062)
                     /label="-10 (placUV5)"
                     /label=""-10 (placUV5)""
     primer          complement(2080..2104)
                     /label=pEX-rev(OR173)
     -35_signal      complement(2081..2086)
                     /label="-35 (placUV5)"
                     /label=""-35 (placUV5)""
     primer          complement(2189..2229)
                     /label=VN207_EFC04(OR243)
     primer          complement(2189..2217)
                     /label=VN207_EFC01(OR240)
     Site            join(2216^2217,2212..2217)
                     /note="Name: XbaI"
                     /note="Pattern: tctaga"
                     /note="cut_0_on_positive_strand: 3922^3923"
                     /note="cut_0_on_negative_strand: 3926^3927"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XbaI
     primer          2218..2250
                     /label=VN207_EFC08(OR247)
     misc_feature    complement(2218..2229)
                     /label="12 bp (50%GC)"
                     /label=""12 bp (50%GC)""
     primer          2230..2250
                     /label=VN207_EFC05(OR244)
     unsure          complement(2240..2284)
                     /vntifkey="31"
                     /label=S-Tag
     Site            join(2295^2296,2291..2296)
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 2902^2903"
                     /note="cut_0_on_negative_strand: 2906^2907"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     primer          2292..2335
                     /label=XhoI_Term_AS(OR213)
     Site            join(2303..2309,2334^2335)
                     /note="Name: SapI"
                     /note="Pattern: gctcttc"
                     /note="cut_0_on_positive_strand: 2822^2823"
                     /note="cut_0_on_negative_strand: 2825^2826"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SapI
     terminator      complement(2336..2375)
                     /label=">Bi-directional terminator: BBa_B1007 Part-only sequence (40 bp)"
                     /label="">Bi-directional terminator: BBa_B1007 Part-only sequence (40 bp)""
     terminator      2336..2375
                     /label=">Bi-directional terminator: BBa_B1007 Part-only sequence (40 bp)"
                     /label="">Bi-directional terminator: BBa_B1007 Part-only sequence (40 bp)""
     Site            join(2393..2400,2397^2398)
                     /note="Name: PacI"
                     /note="Pattern: ttaattaa"
                     /note="cut_0_on_positive_strand: 2397^2398"
                     /note="cut_0_on_negative_strand: 2395^2396"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
                     /label=PacI
     primer          complement(2396..2490)
                     /label=OR717
     primer          2398..2486
                     /label=OR716
     unsure          2398..2486
                     /origin="OL2-62v3_2015-07-23 (129bp linear)Fragment: from
                     PacI cut at 19^20 to BsiWI cut at 108^109,
                     89bpOrientation: originalPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus
                     strand 3':  no change"
                     /label=Fragment
     promoter        2402..2418
                     /label=OL2-62
     promoter        2402..2472
                     /label=pOL2-62_L
     -35_signal      2436..2441
                     /label=-35
     -10_signal      2460..2465
                     /label=-10
     misc_signal     2472
                     /label="Transcription_start (+1)"
                     /label="Transcription_start (+1)"
                     /label=""Transcription_start (+1)""
     RBS             2472..2491
     Site            join(2486..2491,2486^2487)
                     /note="Name: BsiWI"
                     /note="Pattern: cgtacg"
                     /note="cut_0_on_positive_strand: 2486^2487"
                     /note="cut_0_on_negative_strand: 2490^2491"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BsiWI
     CDS             2492..3211
                     /label=eGFP
     primer          complement(2522..2545)
                     /label=eGFPNt_AS(OR207)
     misc_binding    3160..3167
                     /label=CRP
     -35_signal      3182..3186
                     /label=-35?
     -10_signal      3203..3207
                     /label=-10?
     misc_signal     3209..3211
                     /label=Stop
     primer          complement(3215..3239)
                     /label=Ouv_VN305_AS
     RBS             3223..3226
     CDS             3240..4055
                     /label=KanR
     primer          complement(3249..3268)
                     /label=OR179
     primer          complement(4030..4055)
                     /label=OR510
     primer          complement(4034..4055)
                     /label=OR193
     RBS             4039..4042
     primer          4056..4088
                     /label=Ouv_VN305_S
                     /label=Ouv_VN305_S(OR466)
     rep_origin      complement(4157..4597)
                     /label=f1
     terminator      4330..4344
                     /label=Predicted_terminator_5'-Tail
     terminator      4345..4394
                     /label="Predicted_terminator_Stem-loop (http://pepper.molgenrug.nl/index.php/pepper-tools/terminator-predictie)"
                     /label=""Predicted_terminator_Stem-loop (http://pepper.molgenrug.nl/index.php/pepper-tools/terminator-predictie)""
     terminator      4395..4409
                     /label=Predicted_terminator_3'-Tail
     primer          complement(4591..4607)
                     /label=f1_S(OR192)
     Site            join(4605^4606,4601..4606)
                     /note="Name: BglII"
                     /note="Pattern: agatct"
                     /note="cut_0_on_positive_strand: 3984^3985"
                     /note="cut_0_on_negative_strand: 3988^3989"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BglII
     terminator      4607..4701
                     /label=">Bi-directional terminator: BBa_B0014 Part-only sequence (95 bp)"
                     /label="">Bi-directional terminator: BBa_B0014 Part-only sequence (95 bp)""
     terminator      complement(4607..4701)
                     /label=">Bi-directional terminator: BBa_B0014 Part-only sequence (95 bp)"
                     /label="">Bi-directional terminator: BBa_B0014 Part-only sequence (95 bp)""
     Region          4607..4652
                     /note="Match:
                     AAATAATAAAAAAGCCGGATTAATAATCTGGCTTTTTATATTCTCT"
                     /note="Length of match: 46"
                     /note="Accuracy (%): 100"
                     /note="Found at strand: Positive"
                     /label=BBa_B0021
     Region          complement(4607..4652)
                     /note="Match:
                     AGAGAATATAAAAAGCCAGATTATTAATCCGGCTTTTTTATTATTT"
                     /note="Length of match: 46"
                     /note="Accuracy (%): 100"
                     /note="Found at strand: Negative"
                     /label=BBa_B0011
     primer          complement(4702..4728)
                     /label=VN207_EFC06(OR245)
     primer          4717..4765
                     /label=VN207_EFC02(OR241)
     misc_feature    complement(4717..4728)
                     /label="12 bp (50%GC)"
                     /label=""12 bp (50%GC)""
     Site            join(4732^4733,4728..4733)
                     /note="Name: XbaI"
                     /note="Pattern: tctaga"
                     /note="cut_0_on_positive_strand: 1424^1425"
                     /note="cut_0_on_negative_strand: 1428^1429"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XbaI
     primer          4729..4765
                     /label=VN207_EFC03(OR242)
     rep_origin      4764..5309
                     /label=ori
                     /label=p15A
     primer          complement(4879..4895)
                     /label=VN194_ori(OR248)
     CDS             5938..6156
                     /label=CmR
     Site            join(6155^6156,6151..6156)
                     /note="Name: EcoRI"
                     /note="Pattern: gaattc"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=EcoRI
ORIGIN
        1 CGTATGGCAA TGAAAGACGG TGAGCTGGTG ATATGGGATA GTGTTCACCC TTGTTACACC
       61 GTTTTCCATG AGCAAACTGA AACGTTTTCA TCGCTCTGGA GTGAATACCA CGACGATTTC
      121 CGGCAGTTTC TACACATATA TTCGCAAGAT GTGGCGTGTT ACGGTGAAAA CCTGGCCTAT
      181 TTCCCTAAAG GGTTTATTGA GAATATGTTT TTCGTCTCAG CCAATCCCTG GGTGAGTTTC
      241 ACCAGTTTTG ATTTAAACGT GGCCAATATG GACAACTTCT TCGCCCCCGT TTTCACCATG
      301 GGCAAATATT ATACGCAAGG CGACAAGGTG CTGATGCCGC TGGCGATTCA GGTTCATCAT
      361 GCCGTCTGTG ATGGCTTCCA TGTCGGCAGA ATGCTTAATG AATTACAACA GTACTGCGAT
      421 GAGTGGCAGG GCGGGGCGTA ATTTTTTTAA GGCAGTTATT GGTGCCCTTA AACGCCTGGT
      481 GCTACGCCTG AATAAGTGAT AATAAGCGGA TGAATGGCAG AAATTCGAAA GCAAATTCGA
      541 CCCGGTCGTC GGTTCAGGGC AGGGTCGTTA AATAGCCGCT TATGTCTATT GCTGGTTTAC
      601 CGGTTTATTG ACTACCGGAA GCAGTGTGAC CGTGTGCTTC TCAAATGCCT GAGGCCAGTT
      661 TGCTCAGGCT CTCCCCGTGG AGGTAATAAT TGACGATATG ATCATTTATT CTGCCTCCCA
      721 GAGCCTGATA AAAACGGTTA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC
      781 CAGCAGCATC CTGCGATGCA GATCTGTAAG GTAAGGATCC TTATCAGTTA TCTTCCCAAT
      841 TAATGTGGAA TCTTGTGACC CTGGGATTAG ATGCCAAAAT ATTCCTTTGA AGCTTAGAAA
      901 AGTCTGGTTT TACTGGTGGA TTTTCCCTTA ATTCTGTCTC AGTATATTCA TTATTTACAT
      961 AATAGCCAAC TCTAATAAAT TCTTGTCCTC GATAGGTACA AGTAATTAGC ACAACAGTTA
     1021 CGCCTACTGC ATCTGCATCT GGAATGAGTC CTGGATTAGG TGCATCAGCC TGAAATACAA
     1081 ACATATGCCT TCCTGCGGGA ACAGGACCCA CTAAAACAGA GTCTAAAACT TGATCGTATT
     1141 CTTCACTTTC TGCAGAGCCC ACATAGATAA TTTTCCATTC CAAGTCTTCA GACAGGTCCT
     1201 CGATGCACTC GAAGGTGATC TCGAACTGGA ACGGGTTGTA GAAAGGAGAA GGGTTATCCA
     1261 GCACCACTAC ATTGTTCACC TGAACCTTTG CTGCGGCCGC GCCAAACGTC TCTTCAGGCC
     1321 ACTGACTAGC GATAACTTTC CCCACAACGG AACAACTCTC ATTGCATGGG ATCATTGGGT
     1381 ACTGTGGGTT TAGTGGTTGT AAAAACACCT GACCGCTATC CCTGATCAGT TTCTTGAAGG
     1441 TAAACTCATC ACCCCCAAGT CTGGCTATGC AGAAATCACC TGGCTCAACA GCCTGCTCAG
     1501 GGTCAACGAG AATTAACATT CCGTCAGGAA AGCTTGGCTT GGAGCCTGTT GGTGCGGTCA
     1561 TGGAATTACC TTCAACCTCA AGCCAGAATG CAGAATCACT GGCTTTTTTG GTTGTGCTTA
     1621 CCCATCTCTC CGCATCACCT TTGGTAAAGG TTCTAAGCTC AGGTGAGAAC ATCCCTGCCT
     1681 GAACATGAGA AAAAACAGGG TACTCATACT CACTTCTAAG TGACGGCTGC ATACTAACCG
     1741 CTTCATACAT CTCGTAGATT TCTCTGGCGA TTGAAGGGCT AAATTCTTCA ACGCTAACTT
     1801 TGAGAATTTT TGCAAGCAAT GCGGCGTTAT AAGCATTTAA TGCATTGATG CCATTAAATA
     1861 AAGCACCAAC GCCTGACTGC CCCATCCCCA TCTTGTCTGC GACAGACGGC TGGGATAAGC
     1921 CAAGTTCATT TTTCTTTTTT TCATAAATTG CTTTAAGGCG ACGTGCGTCC TCAAGCTGCT
     1981 CTTGTGTTAA TGGTTTCTTT TTTGTGCTCA TACGCTGTTT CCTGTGTGAA ATTGTTATCC
     2041 GCTCACAATT CCACACATTA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA
     2101 ATGAGTGAGA ATTAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTAC AACTTATATC
     2161 GTATGGGGCT GACTTCAGGT GCTACATTTG AAGAGATAAA TTGCACTGAA ATCTAGAATG
     2221 TCTCCAGACC GCTAGTAGAC GAGTCCATGT GCTGGCGTTC AAATTTCGCA GCAGCGGTTT
     2281 CTTTACCAGA CTCGAGAGCG AGGCTCTTCA AGCTCGCAAA ATTGCTCTTC AAGCTCGCAA
     2341 AAAACCCCGC CCCTGACAGG GCGGGGTTTT TTCGCGTTCG ATCCCGCGAA ATTTAATTAA
     2401 CCAACACCGC CAGAGATACA TTAGGCACCC CAGGCTTGAC ACTTTATGCT TCCGGCTCGG
     2461 ATACTGTGTG GAATTGAAAG AGGAACGTAC GATGGTCAGT AAAGGCGAAG AGTTGTTTAC
     2521 GGGTGTAGTA CCGATTTTAG TGGAACTCGA TGGAGATGTG AATGGGCATA AATTTTCGGT
     2581 TTCAGGTGAA GGTGAAGGAG ATGCCACCTA TGGCAAACTG ACGCTGAAGT TCATCTGTAC
     2641 GACAGGCAAA CTGCCTGTTC CGTGGCCAAC ACTGGTTACT ACGCTGACCT ATGGCGTTCA
     2701 ATGCTTTTCG CGTTATCCGG ACCACATGAA ACAGCATGAC TTCTTTAAAT CCGCGATGCC
     2761 TGAAGGTTAC GTACAAGAAC GCACCATCTT CTTCAAAGAT GACGGGAACT ACAAAACCCG
     2821 TGCAGAGGTC AAATTTGAGG GTGACACCCT TGTGAATCGG ATTGAGCTGA AAGGCATTGA
     2881 CTTTAAAGAA GATGGCAACA TTCTGGGTCA TAAACTGGAG TACAACTACA ACAGCCATAA
     2941 CGTGTATATC ATGGCGGACA AACAGAAGAA TGGGATCAAA GTGAATTTCA AGATTCGCCA
     3001 CAACATTGAA GATGGTAGTG TGCAGTTAGC CGACCACTAT CAGCAGAATA CCCCGATTGG
     3061 CGATGGCCCA GTCTTGCTGC CGGATAATCA TTACCTTAGC ACCCAATCTG CGTTAAGCAA
     3121 AGATCCCAAC GAAAAGCGCG ATCACATGGT CCTCCTGGAA TTTGTGACTG CTGCAGGCAT
     3181 CACTTTGGGT ATGGATGAAC TGTATAAGTA AACGCGTTAA ACAGGAAACA GAAGTCCAAA
     3241 TGAGCCATAT TCAACGGGAA ACGTCTTGCT CTAGGCCGCG ATTAAATTCC AACATGGATG
     3301 CTGATTTATA TGGGTATAAA TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT
     3361 ATCGATTGTA TGGGAAGCCC GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG
     3421 TTGCCAATGA TGTTACAGAT GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC
     3481 TTCCGACCAT CAAGCATTTT ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA
     3541 TCCCCGGGAA AACAGCATTC CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG
     3601 TTGATGCGCT GGCAGTGTTC CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT
     3661 TTAACAGCGA TCGCGTATTT CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG
     3721 TTGATGCGAG TGATTTTGAT GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG
     3781 AAATGCATAA ACTTTTGCCA TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC
     3841 TTGATAACCT TATTTTTGAC GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG
     3901 GAATCGCAGA CCGATACCAG GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC
     3961 CTTCATTACA GAAACGGCTT TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT
     4021 TGCAGTTTCA TTTGATGCTC GATGAGTTTT TCTAAGAATT AATTCATGAG CGGATACATA
     4081 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG
     4141 CCACCTAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA
     4201 GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA
     4261 CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG
     4321 ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT
     4381 CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG
     4441 GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA
     4501 AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA
     4561 CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC AGATCTAAAT AATAAAAAAG
     4621 CCGGATTAAT AATCTGGCTT TTTATATTCT CTCTCTAGTA TATAAACGCA GAAAGGCCCA
     4681 CCCGAAGGTG AGCCAGTGTG ACGAACCCCC GCCTCAGAGT GGGAAAGTCT AGAAATATTT
     4741 TATCTGATTA ATAAGATGAT CTTCTTGAGA TCGTTTTGGT CTGCGCGTAA TCTCTTGCTC
     4801 TGAAAACGAA AAAACCGCCT TGCAGGGCGG TTTTTCGAAG GTTCTCTGAG CTACCAACTC
     4861 TTTGAACCGA GGTAACTGGC TTGGAGGAGC GCAGTCACCA AAACTTGTCC TTTCAGTTTA
     4921 GCCTTAACCG GCGCATGACT TCAAGACTAA CTCCTCTAAA TCAATTACCA GTGGCTGCTG
     4981 CCAGTGGTGC TTTTGCATGT CTTTCCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG
     5041 CGCAGCGGTC GGACTGAACG GGGGGTTCGT GCATACAGTC CAGCTTGGAG CGAACTGCCT
     5101 ACCCGGAACT GAGTGTCAGG CGTGGAATGA GACAAACGCG GCCATAACAG CGGAATGACA
     5161 CCGGTAAACC GAAAGGCAGG AACAGGAGAG CGCACGAGGG AGCCGCCAGG GGAAACGCCT
     5221 GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCACTGATT TGAGCGTCAG ATTTCGTGAT
     5281 GCTTGTCAGG GGGGCGGAGC CTATGGAAAA ACGGCTTTGC CGCGGCCCTC TCACTTCCCT
     5341 GTTAAGTATC TTCCTGGCAT CTTCCAGGAA ATCTCCGCCC CGTTCGTAAG CCATTTCCGC
     5401 TCGCCGCAGT CGAACGACCG AGCGTAGCGA GTCAGTGAGC GAGGAAGCGG AATATATCCT
     5461 GTATCACATA TTCTGCTGAC GCACCGGTGC AGCCTTTTTT CTCCTGCCAC ATGAAGCACT
     5521 TCACTGACAC CCTCATCAGT GCCAACATAG TAAGCCAGTA TACACTCCGC TAGCGCTGAT
     5581 GTCCGGCGGT GCTTTTGCCG TTACGCACCA CCCCGTCAGT AGCTGAACAG GAGGGACAGC
     5641 TGATAGAAAC AGAAGCCACT GGAGCACCTC AAAAACACCA TCATACACTA ATCAGTAAGT
     5701 TGGCAGCATT CACCCGACGC ACTTTGCGCC GAATAAATAC CTGTGACGGA AGATCACTTC
     5761 GCAGAATAAA TAAATCCTGG TGTCCCTGTT GATACCGGGA AGCCCTGGGC CAACTTTTGG
     5821 CGAAAATGAG ACGTTGATCG GCACGTAAGA GGTTCCAACT TTCACCATAA TGAAATAAGA
     5881 TCACTACCGG GCGTATTTTT TGAGTTATCG AGATTTTCAG GAGCTAAGGA AGCTAAAATG
     5941 GAGAAAAAAA TCACTGGATA TACCACCGTT GATATATCCC AATGGCATCG TAAAGAACAT
     6001 TTTGAGGCAT TTCAGTCAGT TGCTCAATGT ACCTATAACC AGACCGTTCA GCTGGATATT
     6061 ACGGCCTTTT TAAAGACCGT AAAGAAAAAT AAGCACAAGT TTTATCCGGC CTTTATTCAC
     6121 ATTCTTGCCC GCCTGATGAA TGCTCATCCG GAATTC
//
