LOCUS       VN1272pB2Hws_v28_D      5777 bp    DNA     circular UNA UNA
FEATURES             Location/Qualifiers
     Region          complement(99..114)
                     /note="Match: AAGTTGGCCCAGGGCT"
                     /note="Length of match: 16"
                     /note="Accuracy (%): 100"
                     /note="Description: pAC_Seq"
                     /note="Found at strand: Negative"
                     /label=OR1099
     -35_signal      141..146
                     /label=-35
     -10_signal      164..169
                     /label=-10
     Region          complement(212..235)
                     /note="Match: TTTAGCTTCCTTAGCTCCTGAAAA"
                     /note="Length of match: 24"
                     /note="Accuracy (%): 100"
                     /note="Description: pAC_dCm_S"
                     /note="Found at strand: Negative"
                     /label=OR355
     RBS             225..228
     Region          236..263
                     /note="Match: ATGGAGAAAAAAATCACTGGATATACCA"
                     /note="Length of match: 28"
                     /note="Accuracy (%): 100"
                     /note="Description: Cm_S"
                     /note="Found at strand: Positive"
                     /label=OR383
     CDS             236..895
                     /label=CmR
     unsure          join(453^454,449..454)
                     /note="Name: EcoRI"
                     /note="Pattern: gaattc"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=EcoRI
     Region          complement(882..895)
                     /note="Match: TTACGCCCCGCCCT"
                     /note="Length of match: 14"
                     /note="Accuracy (%): 100"
                     /note="Description: Cm_AS"
                     /note="Found at strand: Negative"
                     /label=OR384
     Region          882..930
                     /note="Match:
                     AGGGCGGGGCGTAATTTTTTTAAGGCAGTTATTGGTGCCCTTAAACGCC"
                     /note="Length of match: 49"
                     /note="Accuracy (%): 100"
                     /note="Description: Cm3end_AS"
                     /note="Found at strand: Positive"
                     /label=OR386
     Region          896..920
                     /note="Match: TTTTTTTAAGGCAGTTATTGGTGCC"
                     /note="Length of match: 25"
                     /note="Accuracy (%): 100"
                     /note="Description: pAC_dCm_AS"
                     /note="Found at strand: Positive"
                     /label=OR356
     Region          complement(927..941)
                     /note="Match: GCGTAGCACCAGGCG"
                     /note="Length of match: 15"
                     /note="Accuracy (%): 100"
                     /note="Description: VN360_S"
                     /note="Found at strand: Negative"
                     /label=OR480
     Region          complement(1038..1059)
                     /note="Match: AACCGGTAAACCAGCAATAGAC"
                     /note="Length of match: 22"
                     /note="Accuracy (%): 100"
                     /note="Description: OPN_VN174_S"
                     /note="Found at strand: Negative"
                     /label=OR770
     Region          1126..1148
                     /note="Match: TCCCCGTGGAGGTAATAATTGAC"
                     /note="Length of match: 23"
                     /note="Accuracy (%): 100"
                     /note="Description: pAC-cI_Rev"
                     /note="Found at strand: Positive"
                     /label=OR237
     unsure          join(1157^1158,1153..1158)
                     /note="Name: BclI"
                     /note="Pattern: tgatca"
                     /note="cut_0_on_positive_strand: 3150^3151"
                     /note="cut_0_on_negative_strand: 3154^3155"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BclI
     Region          complement(1219..1253)
                     /note="Match: GCATCGCAGGATGCTGCTGGCTACCCTGTGGAACA"
                     /note="Length of match: 35"
                     /note="Accuracy (%): 100"
                     /note="Description: VN278FRAG1_S"
                     /note="Found at strand: Negative"
                     /label=OR336
     Region          1245..1265
                     /note="Match: CTGCGATGCAGATCTGTAAGG"
                     /note="Length of match: 21"
                     /note="Accuracy (%): 100"
                     /note="Description: cI-fusion_AS"
                     /note="Found at strand: Positive"
                     /label=OR757
     Region          1245..1265
                     /note="Match: CTGCGATGCAGATCTGTAAGG"
                     /note="Length of match: 21"
                     /note="Accuracy (%): 100"
                     /note="Description: VN881_F2_S"
                     /note="Found at strand: Positive"
                     /label=OR1396
     unsure          join(1258^1259,1254..1259)
                     /note="Name: BglII"
                     /note="Pattern: agatct"
                     /note="cut_0_on_positive_strand: 3049^3050"
                     /note="cut_0_on_negative_strand: 3053^3054"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BglII
     primer          complement(1270..1330)
                     /label=OR1764
     primer          1274..1326
                     /label=OR1765
     unsure          1274..1326
                     /origin="Amplicon_OR1764-OR1765_C2S selection (93bp
                     linear)Fragment: from SacI cut at 23^24 to Acc65I cut at
                     76^77, 53bpOrientation: originalPlus strand 5':   no
                     changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label=Fragment
     unsure          1274..1326
                     /origin="VN1182_pB2Hws_v13_cI_2GG+rpoA_IP3 (7�104bp
                     circular)Fragment: from SacI cut at 1273^1274 to Acc65I
                     cut at 1326^1327, 53bpOrientation: originalPlus strand
                     5':   no changePlus strand 3':   no changeMinus strand
                     5':  no changeMinus strand 3':  no change"
                     /label=Fragment
     unsure          complement(1275..1277)
                     /label=Stop
     unsure          complement(1278..1280)
                     /label=Stop
     unsure          join(1281^1282,1287..1292)
                     /note="Name: BsaI"
                     /note="Pattern: ggtctc"
                     /note="cut_0_on_positive_strand: 1281^1282"
                     /note="cut_0_on_negative_strand: 1285^1286"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BsaI
     Region          1293..1312
                     /label=calmodulin(55%GC)
     unsure          join(1313..1318,1323^1324)
                     /note="Name: BsaI"
                     /note="Pattern: ggtctc"
                     /note="cut_0_on_positive_strand: 1287^1288"
                     /note="cut_0_on_negative_strand: 1291^1292"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BsaI
     unsure          complement(1317..1319)
                     /label=Stop
     CDS             complement(<1331..2042)
                     /label="Asf1B_full lenght"
                     /label=cI_NTD-Asf1B_Short
                     /label=cI-Asf1B
                     /label=""Asf1B_full lenght""
     Region          complement(1332..2042)
                     /label=cI
     primer          complement(1332..1350)
                     /label=OR756
     Region          complement(1439..1458)
                     /note="Match: GATCAGGGATAGCGGTCAGG"
                     /note="Length of match: 20"
                     /note="Accuracy (%): 100"
                     /note="Description: pAC-cI_F"
                     /note="Found at strand: Negative"
                     /label=OR236
     Region          1439..1458
                     /note="Match: CCTGACCGCTATCCCTGATC"
                     /note="Length of match: 20"
                     /note="Accuracy (%): 100"
                     /note="Description: ciE34P_Seq"
                     /note="Found at strand: Positive"
                     /label=OR679
     Region          1917..1951
                     /note="Match: CCCCATCTTGTCTGCGACAGACGGCTGGGATAAGC"
                     /note="Length of match: 35"
                     /note="Accuracy (%): 100"
                     /note="Description: cI_E34P_AS"
                     /note="Found at strand: Positive"
                     /label=OR132
     Region          complement(1927..1966)
                     /note="Match: AGAAAAATGAACTTGGCTTATCCCAGCCGTCTGTCGCAGA"
                     /note="Length of match: 40"
                     /note="Accuracy (%): 100"
                     /note="Description: cI_E34P_S"
                     /note="Found at strand: Negative"
                     /label=OR131
     Region          1927..1950
                     /note="Match: TCTGCGACAGACGGCTGGGATAAG"
                     /note="Length of match: 24"
                     /note="Accuracy (%): 100"
                     /note="Description: PrVN529_S"
                     /note="Found at strand: Positive"
                     /label=OR677
     Region          complement(1927..1950)
                     /note="Match: CTTATCCCAGCCGTCTGTCGCAGA"
                     /note="Length of match: 24"
                     /note="Accuracy (%): 100"
                     /note="Description: PrVN529_AS"
                     /note="Found at strand: Negative"
                     /label=OR678
     mutation        complement(1938..1940)
                     /label=E34P
     Region          1972..1996
                     /note="Match: TCATAAATTGCTTTAAGGCGACGTG"
                     /note="Length of match: 25"
                     /note="Accuracy (%): 100"
                     /note="Description: cI_AS2"
                     /note="Found at strand: Positive"
                     /label=OR1420
     Region          complement(2006..2042)
                     /note="Match: ATGAGCACAAAAAAGAAACCATTAACACAAGAGCAGC"
                     /note="Length of match: 37"
                     /note="Accuracy (%): 100"
                     /note="Description: VN881_SalI_AS"
                     /note="Found at strand: Negative"
                     /label=OR1449
     RBS             complement(2043..2066)
                     /label=WeakRBSLib
     unsure          join(2067..2072,2067^2068)
                     /note="Name: BspTI"
                     /note="Pattern: cttaag"
                     /note="cut_0_on_positive_strand: 2490^2491"
                     /note="cut_0_on_negative_strand: 2494^2495"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BspTI
     promoter        complement(2073..2108)
                     /label=pL
     unsure          complement(2073)
                     /label=+1
     -10_signal      complement(2080..2085)
                     /label=-10
     unsure          complement(2084..2102)
                     /label=TetO
     -35_signal      complement(2103..2108)
                     /label=-35
     unsure          join(2109..2114,2109^2110)
                     /note="Name: PteI"
                     /note="Pattern: gcgcgc"
                     /note="cut_0_on_positive_strand: 2532^2533"
                     /note="cut_0_on_negative_strand: 2536^2537"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
                     /label=PteI
     primer          complement(2115..2142)
                     /label=OR476
     primer          complement(2116..2144)
                     /label=OR240
     primer          complement(2116..2155)
                     /label=OR243
     primer          2144..2176
                     /label=OR247
     primer          2156..2176
                     /label=OR244
     unsure          join(2181^2182,2177..2182)
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 2902^2903"
                     /note="cut_0_on_negative_strand: 2906^2907"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     primer          2178..2221
                     /label=XhoI_Term_AS(OR213)
     Region          2183..2200
                     /note="Match: AGCGAGGCTCTTCAAGCT"
                     /note="Length of match: 18"
                     /note="Accuracy (%): 100"
                     /note="Description: SapI_2_OR2"
                     /note="Found at strand: Positive"
                     /label=OR204
     unsure          join(2189..2195,2196^2197)
                     /note="Name: SapI"
                     /note="Pattern: gctcttc"
                     /note="cut_0_on_positive_strand: 2310^2311"
                     /note="cut_0_on_negative_strand: 2313^2314"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SapI
     primer          complement(2191..2211)
                     /label=OR1912
     unsure          join(2210..2216,2217^2218)
                     /note="Name: SapI"
                     /note="Pattern: gctcttc"
                     /note="cut_0_on_positive_strand: 2331^2332"
                     /note="cut_0_on_negative_strand: 2334^2335"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SapI
     terminator      complement(2222..2261)
                     /label=">Bi-directional terminator: BBa_B1007
                     Part-onlysequence (40 bp)"
                     /label=">Bi-directional terminator: BBa_B1007 Part-only
                     sequence (40 bp)"
                     /label=">Bi-directional terminator: BBa_B1007 Part-only
                     sequence (40 bp)"
                     /label="">Bi-directional terminator: BBa_B1007
                     Part-onlysequence (40 bp)""
     terminator      2222..2261
                     /label=">Bi-directional terminator: BBa_B1007
                     Part-onlysequence (40 bp)"
                     /label=">Bi-directional terminator: BBa_B1007 Part-only
                     sequence (40 bp)"
                     /label=">Bi-directional terminator: BBa_B1007 Part-only
                     sequence (40 bp)"
                     /label="">Bi-directional terminator: BBa_B1007
                     Part-onlysequence (40 bp)""
     primer          2262..2285
                     /label=OR390
     primer          2264..2287
                     /label=OR1864
     primer          2264..2339
                     /label=OR1983
     unsure          join(2279..2286,2283^2284)
                     /note="Name: PacI"
                     /note="Pattern: ttaattaa"
                     /note="cut_0_on_positive_strand: 2706^2707"
                     /note="cut_0_on_negative_strand: 2704^2705"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
                     /label=PacI
     promoter        2288..2304
                     /label=OL2-62
     unsure          2316..2318
                     /label=dcm_mutation
     -35_signal      2322..2327
                     /label=-35
     primer          2342..2397
                     /label=OR1861
     Region          2342..2363
                     /label=Tm=64�C
     -10_signal      2346..2351
                     /label=-10
     RBS             2358..2377
                     /label=RBSlib_weak(T.I.R.=51.3)c1p
     unsure          2358
                     /label="Transcription_start (+1)"
                     /label="Transcription_start (+1)"
                     /label="Transcription_start (+1)"
                     /label="Transcription_start (+1)"
                     /label=""Transcription_start (+1)""
     Region          2378..2397
                     /label=Tm=63�C
     CDS             2378..3193
                     /label=KanR
     Region          complement(3167..3193)
                     /label=Tm=63�C
     primer          complement(3184..3216)
                     /label=OR1865
     unsure          3191..3193
                     /label=Stop
     unsure          join(3195..3200,3195^3196)
                     /note="Name: SpeI"
                     /note="Pattern: actagt"
                     /note="cut_0_on_positive_strand: 3618^3619"
                     /note="cut_0_on_negative_strand: 3622^3623"
                     /note="inhibited_by: "
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SpeI
     primer          3201..3233
                     /label=OR466
     Region          complement(3222..3257)
                     /note="Match: CCTATTTGTTTATTTTTCTAAATACATTCAAATATG"
                     /note="Length of match: 36"
                     /note="Accuracy (%): 100"
                     /note="Description: pUC_Frag1_S"
                     /note="Found at strand: Negative"
                     /label=OR733
     unsure          complement(3302..3742)
                     /label=f1
     Region          complement(3359..3377)
                     /note="Match: GCCGATTTCGGCCTATTGG"
                     /note="Length of match: 19"
                     /note="Accuracy (%): 100"
                     /note="Description: Seq_finf1_S"
                     /note="Found at strand: Negative"
                     /label=OR471
     terminator      3475..3489
                     /label=Predicted_terminator_5'-Tail
     terminator      3490..3539
                     /label="Predicted_terminator_Stem-loop(http://pepper.molge
                     nrug.nl/index.php/pepper-tools/terminator-predictie)"
                     /label="Predicted_terminator_Stem-loop
                     (http://pepper.molgenrug.nl/index.php/pepper-tools/termina
                     tor-predictie)"
                     /label="Predicted_terminator_Stem-loop
                     (http://pepper.molgenrug.nl/index.php/pepper-tools/termina
                     tor-predictie)"
                     /label=""Predicted_terminator_Stem-loop(http://pepper.molge
                     nrug.nl/index.php/pepper-tools/terminator-predictie)""
     terminator      3540..3554
                     /label=Predicted_terminator_3'-Tail
     Region          complement(3736..3752)
                     /note="Match: TAGATCTGCGCCCTGTA"
                     /note="Length of match: 17"
                     /note="Accuracy (%): 100"
                     /note="Description: f1_S"
                     /note="Found at strand: Negative"
                     /label=OR192
     unsure          join(3750^3751,3746..3751)
                     /note="Name: BglII"
                     /note="Pattern: agatct"
                     /note="cut_0_on_positive_strand: 3984^3985"
                     /note="cut_0_on_negative_strand: 3988^3989"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BglII
     terminator      3752..3846
                     /label=">Bi-directional terminator: BBa_B0014
                     Part-onlysequence (95 bp)"
                     /label=">Bi-directional terminator: BBa_B0014 Part-only
                     sequence (95 bp)"
                     /label=">Bi-directional terminator: BBa_B0014 Part-only
                     sequence (95 bp)"
                     /label="">Bi-directional terminator: BBa_B0014
                     Part-onlysequence (95 bp)""
     terminator      complement(3752..3846)
                     /label=">Bi-directional terminator: BBa_B0014
                     Part-onlysequence (95 bp)"
                     /label=">Bi-directional terminator: BBa_B0014 Part-only
                     sequence (95 bp)"
                     /label=">Bi-directional terminator: BBa_B0014 Part-only
                     sequence (95 bp)"
                     /label="">Bi-directional terminator: BBa_B0014
                     Part-onlysequence (95 bp)""
     Region          3752..3797
                     /note="Match:
                     AAATAATAAAAAAGCCGGATTAATAATCTGGCTTTTTATATTCTCT"
                     /note="Length of match: 46"
                     /note="Accuracy (%): 100"
                     /note="Found at strand: Positive"
                     /label=BBa_B0021
     Region          complement(3752..3797)
                     /note="Match:
                     AGAGAATATAAAAAGCCAGATTATTAATCCGGCTTTTTTATTATTT"
                     /note="Length of match: 46"
                     /note="Accuracy (%): 100"
                     /note="Found at strand: Negative"
                     /label=BBa_B0011
     Region          complement(3752..3782)
                     /note="Match: CCAGATTATTAATCCGGCTTTTTTATTATTT"
                     /note="Length of match: 31"
                     /note="Accuracy (%): 100"
                     /note="Description: ISceI_AS"
                     /note="Found at strand: Negative"
                     /label=OR169
     Region          complement(3829..3846)
                     /note="Match: TCACACTGGCTCACCTTC"
                     /note="Length of match: 18"
                     /note="Accuracy (%): 100"
                     /note="Description: DtRNA_AS"
                     /note="Found at strand: Negative"
                     /label=OR148
     Region          3829..3846
                     /note="Match: GAAGGTGAGCCAGTGTGA"
                     /note="Length of match: 18"
                     /note="Accuracy (%): 100"
                     /note="Description: B0014_AS"
                     /note="Found at strand: Positive"
                     /label=OR588
     Region          complement(3847..3861)
                     /note="Match: TGAGGCGGGGGTTCG"
                     /note="Length of match: 15"
                     /note="Accuracy (%): 100"
                     /note="Description: VN207_EFC07"
                     /note="Found at strand: Negative"
                     /label=OR246
     Region          complement(3847..3873)
                     /note="Match: ACTTTCCCACTCTGAGGCGGGGGTTCG"
                     /note="Length of match: 27"
                     /note="Accuracy (%): 100"
                     /note="Description: VN207_EFC06"
                     /note="Found at strand: Negative"
                     /label=OR245
     primer          3862..3910
                     /label=VN207_EFC02(OR241)
     unsure          join(3877^3878,3873..3878)
                     /note="Name: XbaI"
                     /note="Pattern: tctaga"
                     /note="cut_0_on_positive_strand: 1424^1425"
                     /note="cut_0_on_negative_strand: 1428^1429"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XbaI
     Region          3874..3910
                     /note="Match: CTAGAAATATTTTATCTGATTAATAAGATGATCTTCT"
                     /note="Length of match: 37"
                     /note="Accuracy (%): 100"
                     /note="Description: VN207_EFC03"
                     /note="Found at strand: Positive"
                     /label=OR242
     unsure          3909..4455
                     /label=ori
                     /label=p15A
     Region          complement(4024..4040)
                     /note="Match: ACTGCGCTCCTCCAAGC"
                     /note="Length of match: 17"
                     /note="Accuracy (%): 100"
                     /note="Description: VN194_ori"
                     /note="Found at strand: Negative"
                     /label=OR248
     Region          4156..4177
                     /note="Match: TGGACTCAAGACGATAGTTACC"
                     /note="Length of match: 22"
                     /note="Accuracy (%): 100"
                     /note="Description: Ori"
                     /note="Found at strand: Positive"
                     /label=OR187
     Region          4159..4180
                     /note="Match: ACTCAAGACGATAGTTACCGGA"
                     /note="Length of match: 22"
                     /note="Accuracy (%): 100"
                     /note="Description: pUC_Frag3_S"
                     /note="Found at strand: Positive"
                     /label=OR737
     misc_feature    4456..4458
                     /label="Replication origin and direction (as specified for pACYC184)"
                     /label=""Replication origin and direction (as specified for pACYC184)""
     primer          4532..4550
                     /label=VN720Seq_S(OR1110)
     mutation        4549
                     /label=G=>T(mutation)
     Region          complement(4550..4583)
                     /note="Match: CACTGACTCGCTACGCTCGGTCGTTCGACTGCGG"
                     /note="Length of match: 34"
                     /note="Accuracy (%): 100"
                     /note="Description: VN278FRAG3_S"
                     /note="Found at strand: Negative"
                     /label=OR340
     Region          complement(4565..4608)
                     /note="Match:
                     GAAGGCACTTATTTTTGATCGTTCGCACTGACTCGCTACGCTCG"
                     /note="Length of match: 44"
                     /note="Accuracy (%): 100"
                     /note="Description: VN692_F1_AS"
                     /note="Found at strand: Negative"
                     /label=OR1027
     Region          4584..4608
                     /note="Match: CGAACGATCAAAAATAAGTGCCTTC"
                     /note="Length of match: 25"
                     /note="Accuracy (%): 100"
                     /note="Description: pBRa_S"
                     /note="Found at strand: Positive"
                     /label=OR329
     Region          complement(4584..4608)
                     /note="Match: GAAGGCACTTATTTTTGATCGTTCG"
                     /note="Length of match: 25"
                     /note="Accuracy (%): 100"
                     /note="Description: VN208_Dplpp_AS"
                     /note="Found at strand: Negative"
                     /label=OR429
     -35_signal      4623..4628
                     /label="-35 (plpp)"
                     /label="-35 (plpp)"
                     /label=""-35 (plpp)""
     -10_signal      4648..4653
                     /label="-10 (plpp)"
                     /label="-10 (plpp)"
                     /label=""-10 (plpp)""
     -35_signal      4695..4700
                     /label="placUV5 -35"
                     /label="placUV5 -35"
                     /label=""placUV5 -35""
     -10_signal      4719..4724
                     /label="-10 (placUV5)"
                     /label="-10 (placUV5)"
                     /label=""-10 (placUV5)""
     unsure          4731..4752
                     /label=lacO
     unsure          4731
                     /label="+1 (placUV5)"
                     /label="+1 (placUV5)"
                     /label=""+1 (placUV5)""
     Region          4733..4749
                     /note="Match: TTGTGAGCGGATAACAA"
                     /note="Length of match: 17"
                     /note="Accuracy (%): 100"
                     /note="Description: pLac"
                     /note="Found at strand: Positive"
                     /label=OR16
     Region          complement(4735..4757)
                     /note="Match: GTGTGAAATTGTTATCCGCTCAC"
                     /note="Length of match: 23"
                     /note="Accuracy (%): 100"
                     /note="Description: ptrc_dRBS_AS"
                     /note="Found at strand: Negative"
                     /label=OR394
     Region          4737..4760
                     /note="Match: GAGCGGATAACAATTTCACACAGG"
                     /note="Length of match: 24"
                     /note="Accuracy (%): 100"
                     /note="Description: M13_rev_-49"
                     /note="Found at strand: Positive"
                     /label=OR60
     Region          4757..4774
                     /note="Match: CAGGAAACAGCTATGACC"
                     /note="Length of match: 18"
                     /note="Accuracy (%): 100"
                     /note="Description: M13_rev_-29"
                     /note="Found at strand: Positive"
                     /label=OR61
     Region          4792..4814
                     /note="Match: GGAACTCTAGACCAAAGAGAGGA"
                     /note="Length of match: 23"
                     /note="Accuracy (%): 100"
                     /note="Description: XbaI_RBS_S"
                     /note="Found at strand: Positive"
                     /label=OR306
     RBS             4811..4814
     Region          4819..5562
                     /label=NTD-rpoA
     CDS             4819..5652
                     /label=rpoA-IP3
     Region          complement(4822..4841)
                     /note="Match: aactctgtcacagaaccctg"
                     /note="Length of match: 20"
                     /note="Accuracy (%): 100"
                     /note="Description: pLTetO_star_AS"
                     /note="Found at strand: Negative"
                     /label=OR427
     Region          4822..4842
                     /note="Match: CAGGGTTCTGTGACAGAGTTT"
                     /note="Length of match: 21"
                     /note="Accuracy (%): 100"
                     /note="Description: VN208_Dplpp_S"
                     /note="Found at strand: Positive"
                     /label=OR428
     Region          5168..5186
                     /note="Match: ACGACGGTGATGTCGAAAT"
                     /note="Length of match: 19"
                     /note="Accuracy (%): 100"
                     /note="Description: RNApol_S1"
                     /note="Found at strand: Positive"
                     /label=OR139
     Region          5395..5415
                     /note="Match: GAACAGCGTACCGACCTGGAC"
                     /note="Length of match: 21"
                     /note="Accuracy (%): 100"
                     /note="Description: pBRa_F"
                     /note="Found at strand: Positive"
                     /label=OR234
     Region          complement(5448..5466)
                     /note="Match: CGCCTCTTCAGGATCGATT"
                     /note="Length of match: 19"
                     /note="Accuracy (%): 100"
                     /note="Description: RNApol_AS1"
                     /note="Found at strand: Negative"
                     /label=OR140
     Region          5448..5466
                     /note="Match: AATCGATCCTGAAGAGGCG"
                     /note="Length of match: 19"
                     /note="Accuracy (%): 100"
                     /note="Description: RNApol_S2"
                     /note="Found at strand: Positive"
                     /label=OR143
     Region          5489..5507
                     /note="Match: TGGCTGAACAACTGGAAGC"
                     /note="Length of match: 19"
                     /note="Accuracy (%): 100"
                     /note="Description: PourVN688_S"
                     /note="Found at strand: Positive"
                     /label=OR1003
     unsure          join(5504..5509,5504^5505)
                     /note="Name: HindIII"
                     /note="Pattern: aagctt"
                     /note="cut_0_on_positive_strand: 1044^1045"
                     /note="cut_0_on_negative_strand: 1048^1049"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=HindIII
     Region          5527..5564
                     /note="Match: GTACGTCAGCCTGAAGTGAAAGAAGAGAAACCAGAGGC"
                     /note="Length of match: 38"
                     /note="Accuracy (%): 100"
                     /note="Description: VN808_S_BsaI"
                     /note="Found at strand: Positive"
                     /label=OR1249
     Region          5549..5564
                     /note="Match: AAGAGAAACCAGAGGCGG"
                     /note="Length of match: 18"
                     /note="Accuracy (%): 100"
                     /note="Description: SIMs_S"
                     /note="Found at strand: Positive"
                     /label=OR804
     Region          5549..5567
                     /note="Match: AAGAGAAACCAGAGGCGGC"
                     /note="Length of match: 19"
                     /note="Accuracy (%): 100"
                     /note="Description: CtLib_S"
                     /note="Found at strand: Positive"
                     /label=OR870
     unsure          join(5563..5570,5564^5565)
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 6818^6819"
                     /note="cut_0_on_negative_strand: 6822^6823"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
     Region          5572..5649
                     /label=IP3
     unsure          5650..5652
                     /label=Stop
     unsure          join(5653..5658,5653^5654)
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="cut_0_on_positive_strand: 6868^6869"
                     /note="cut_0_on_negative_strand: 6872^6873"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     primer          complement(5658..5673)
                     /label=OR906(Tm=63.3)
     Region          complement(5658..5673)
                     /note="Match: TGCGTCCGGCGTAGAG"
                     /note="Length of match: 16"
                     /note="Accuracy (%): 100"
                     /note="Description: NtLib_AS"
                     /note="Found at strand: Negative"
                     /label=OR869
     Region          5658..5751
                     /note="Match:
                     CTCTACGCCGGACGCACTCGGTTCCAAAAAAAAAAAAAAAGACGCTGAAAAGCGTCTT
                     TTTTTTTTTTGGTCCCCACTGGAGCACCTCAAAAAC"
                     /note="Length of match: 94"
                     /note="Accuracy (%): 100"
                     /note="Description: VN692_F3_S"
                     /note="Found at strand: Positive"
                     /label=OR1024
     Region          complement(5658..5751)
                     /note="Match:
                     GTTTTTGAGGTGCTCCAGTGGGGACCAAAAAAAAAAAAGACGCTTTTCAGCGTCTTTT
                     TTTTTTTTTTTGGAACCGAGTGCGTCCGGCGTAGAG"
                     /note="Length of match: 94"
                     /note="Accuracy (%): 100"
                     /note="Description: VN692_F3_AS"
                     /note="Found at strand: Negative"
                     /label=OR1025
     Region          complement(5659..5673)
                     /note="Match: TGCGTCCGGCGTAGA"
                     /note="Length of match: 15"
                     /note="Accuracy (%): 100"
                     /note="Description: SIMs_AS"
                     /note="Found at strand: Negative"
                     /label=OR805
     terminator      5674..5730
                     /label=L3S2P21
     Region          5690..5697
                     /label=A-tract
     unsure          join(5698..5703,5708..5713)
                     /label=Stem
     unsure          5704..5707
                     /label=loop
     Region          5714..5725
                     /label=U-Tract
     Region          5731..5751
                     /note="Match: CCACTGGAGCACCTCAAAAAC"
                     /note="Length of match: 21"
                     /note="Accuracy (%): 100"
                     /note="Description: VN692_F1_S"
                     /note="Found at strand: Positive"
                     /label=OR1026
ORIGIN
        1 GGCAGCATCA CCCGACGCAC TTTGCGCCGA ATAAATACCT GTGACGGAAG ATCACTTCGC
       61 AGAATAAATA AATCCTGGTG TCCCTGTTGA TACCGGGAAG CCCTGGGCCA ACTTTTGGCG
      121 AAAATGAGAC GTTGATCGGC ACGTAAGAGG TTCCAACTTT CACCATAATG AAATAAGATC
      181 ACTACCGGGC GTATTTTTTG AGTTATCGAG ATTTTCAGGA GCTAAGGAAG CTAAAATGGA
      241 GAAAAAAATC ACTGGATATA CCACCGTTGA TATATCCCAA TGGCATCGTA AAGAACATTT
      301 TGAGGCATTT CAGTCAGTTG CTCAATGTAC CTATAACCAG ACCGTTCAGC TGGATATTAC
      361 GGCCTTTTTA AAGACCGTAA AGAAAAATAA GCACAAGTTT TATCCGGCCT TTATTCACAT
      421 TCTTGCCCGC CTGATGAATG CTCATCCGGA ATTCCGTATG GCAATGAAAG ACGGTGAGCT
      481 GGTGATATGG GATAGTGTTC ACCCTTGTTA CACCGTTTTC CATGAGCAAA CTGAAACGTT
      541 TTCATCGCTC TGGAGTGAAT ACCACGACGA TTTCCGGCAG TTTCTACACA TATATTCGCA
      601 AGATGTGGCG TGTTACGGTG AAAACCTGGC CTATTTCCCT AAAGGGTTTA TTGAGAATAT
      661 GTTTTTCGTC TCAGCCAATC CCTGGGTGAG TTTCACCAGT TTTGATTTAA ACGTGGCCAA
      721 TATGGACAAC TTCTTCGCCC CCGTTTTCAC CATGGGCAAA TATTATACGC AAGGCGACAA
      781 GGTGCTGATG CCGCTGGCGA TTCAGGTTCA TCATGCCGTC TGTGATGGCT TCCATGTCGG
      841 CAGAATGCTT AATGAATTAC AACAGTACTG CGATGAGTGG CAGGGCGGGG CGTAATTTTT
      901 TTAAGGCAGT TATTGGTGCC CTTAAACGCC TGGTGCTACG CCTGAATAAG TGATAATAAG
      961 CGGATGAATG GCAGAAATTC GAAAGCAAAT TCGACCCGGT CGTCGGTTCA GGGCAGGGTC
     1021 GTTAAATAGC CGCTTATGTC TATTGCTGGT TTACCGGTTT ATTGACTACC GGAAGCAGTG
     1081 TGACCGTGTG CTTCTCAAAT GCCTGAGGCC AGTTTGCTCA GGCTCTCCCC GTGGAGGTAA
     1141 TAATTGACGA TATGATCATT TATTCTGCCT CCCAGAGCCT GATAAAAACG GTTAGCGCTT
     1201 CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCTG
     1261 TAAGGTAAGA GCTCTTATCA AGATCCGAGA CCGACTTCCC AGAGTTCCTG ACGGTCTCAC
     1321 ATGGCGGTAC CGCCAAACGT CTCTTCAGGC CACTGACTAG CGATAACTTT CCCCACAACG
     1381 GAACAACTCT CATTGCATGG GATCATTGGG TACTGTGGGT TTAGTGGTTG TAAAAACACC
     1441 TGACCGCTAT CCCTGATCAG TTTCTTGAAG GTAAACTCAT CACCCCCAAG TCTGGCTATG
     1501 CAGAAATCAC CTGGCTCAAC AGCCTGCTCA GGGTCAACGA GAATTAACAT TCCGTCAGGA
     1561 AAGCTTGGCT TGGAGCCTGT TGGTGCGGTC ATGGAATTAC CTTCAACCTC AAGCCAGAAT
     1621 GCAGAATCAC TGGCTTTTTT GGTTGTGCTT ACCCATCTCT CCGCATCACC TTTGGTAAAG
     1681 GTTCTAAGCT CAGGTGAGAA CATCCCTGCC TGAACATGAG AAAAAACAGG GTACTCATAC
     1741 TCACTTCTAA GTGACGGCTG CATACTAACC GCTTCATACA TCTCGTAGAT TTCTCTGGCG
     1801 ATTGAAGGGC TAAATTCTTC AACGCTAACT TTGAGAATTT TTGCAAGCAA TGCGGCGTTA
     1861 TAAGCATTTA ATGCATTGAT GCCATTAAAT AAAGCACCAA CGCCTGACTG CCCCATCCCC
     1921 ATCTTGTCTG CGACAGACGG CTGGGATAAG CCAAGTTCAT TTTTCTTTTT TTCATAAATT
     1981 GCTTTAAGGC GACGTGCGTC CTCAAGCTGC TCTTGTGTTA ATGGTTTCTT TTTTGTGCTC
     2041 ATTATATGAC CTAGCGAGCT GGTCTACTTA AGTGCTAGCA GTATCTCTAT CACTGATAGG
     2101 GATGTCAAGC GCGCTTGAAG AGATAAATTG CACTGAAATC TAGATGTCTC CAGACCGCTA
     2161 GTAGACGAGT CCATGTCTCG AGAGCGAGGC TCTTCAAGCT CGCAAAATTG CTCTTCAAGC
     2221 TCGCAAAAAA CCCCGCCCCT GACAGGGCGG GGTTTTTTCG CGTTCGATCC CGCGAAATTT
     2281 AATTAACCAA CACCGCCAGA GATACATTAG GCACCGGCGG CTTGACACTT TATGCTTCCG
     2341 GCTCGGATAC TGTGTGGATT TGACAGCGGC AGGTACGATG AGCCATATTC AACGGGAAAC
     2401 GTCTTGCTCT AGGCCGCGAT TAAATTCCAA CATGGATGCT GATTTATATG GGTATAAATG
     2461 GGCTCGCGAT AATGTCGGGC AATCAGGTGC GACAATCTAT CGATTGTATG GGAAGCCCGA
     2521 TGCGCCAGAG TTGTTTCTGA AACATGGCAA AGGTAGCGTT GCCAATGATG TTACAGATGA
     2581 GATGGTCAGA CTAAACTGGC TGACGGAATT TATGCCTCTT CCGACCATCA AGCATTTTAT
     2641 CCGTACTCCT GATGATGCAT GGTTACTCAC CACTGCGATC CCCGGGAAAA CAGCATTCCA
     2701 GGTATTAGAA GAATATCCTG ATTCAGGTGA AAATATTGTT GATGCGCTGG CAGTGTTCCT
     2761 GCGCCGGTTG CATTCGATTC CTGTTTGTAA TTGTCCTTTT AACAGCGATC GCGTATTTCG
     2821 TCTCGCTCAG GCGCAATCAC GAATGAATAA CGGTTTGGTT GATGCGAGTG ATTTTGATGA
     2881 CGAGCGTAAT GGCTGGCCTG TTGAACAAGT CTGGAAAGAA ATGCATAAAC TTTTGCCATT
     2941 CTCACCGGAT TCAGTCGTCA CTCATGGTGA TTTCTCACTT GATAACCTTA TTTTTGACGA
     3001 GGGGAAATTA ATAGGTTGTA TTGATGTTGG ACGAGTCGGA ATCGCAGACC GATACCAGGA
     3061 TCTTGCCATC CTATGGAACT GCCTCGGTGA GTTTTCTCCT TCATTACAGA AACGGCTTTT
     3121 TCAAAAATAT GGTATTGATA ATCCTGATAT GAATAAATTG CAGTTTCATT TGATGCTCGA
     3181 TGAGTTTTTC TAAGACTAGT GAATTAATTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     3241 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TAAATTGTAA
     3301 GCGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC
     3361 AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA
     3421 GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG
     3481 GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT
     3541 TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA
     3601 GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG
     3661 CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG
     3721 CGCTTAATGC GCCGCTACAG GGCGCAGATC TAAATAATAA AAAAGCCGGA TTAATAATCT
     3781 GGCTTTTTAT ATTCTCTCTC TAGTATATAA ACGCAGAAAG GCCCACCCGA AGGTGAGCCA
     3841 GTGTGACGAA CCCCCGCCTC AGAGTGGGAA AGTCTAGAAA TATTTTATCT GATTAATAAG
     3901 ATGATCTTCT TGAGATCGTT TTGGTCTGCG CGTAATCTCT TGCTCTGAAA ACGAAAAAAC
     3961 CGCCTTGCAG GGCGGTTTTT CGAAGGTTCT CTGAGCTACC AACTCTTTGA ACCGAGGTAA
     4021 CTGGCTTGGA GGAGCGCAGT CACCAAAACT TGTCCTTTCA GTTTAGCCTT AACCGGCGCA
     4081 TGACTTCAAG ACTAACTCCT CTAAATCAAT TACCAGTGGC TGCTGCCAGT GGTGCTTTTG
     4141 CATGTCTTTC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGACT
     4201 GAACGGGGGG TTCGTGCATA CAGTCCAGCT TGGAGCGAAC TGCCTACCCG GAACTGAGTG
     4261 TCAGGCGTGG AATGAGACAA ACGCGGCCAT AACAGCGGAA TGACACCGGT AAACCGAAAG
     4321 GCAGGAACAG GAGAGCGCAC GAGGGAGCCG CCAGGGGGAA ACGCCTGGTA TCTTTATAGT
     4381 CCTGTCGGGT TTCGCCACCA CTGATTTGAG CGTCAGATTT CGTGATGCTT GTCAGGGGGG
     4441 CGGAGCCTAT GGAAAAACGG CTTTGCCGCG GCCCTCTCAC TTCCCTGTTA AGTATCTTCC
     4501 TGGCATCTTC CAGGAAATCT CCGCCCCGTT CGTAAGCCAT TTCCGCTCTC CGCAGTCGAA
     4561 CGACCGAGCG TAGCGAGTCA GTGCGAACGA TCAAAAATAA GTGCCTTCCC ATCAAAAAAA
     4621 TATTGACAAC ATAAAAAACT TTGTGTTATA CTTGTAACGC TACATGGAGA TTAACTCAAT
     4681 CTAGCTAGAG AGGCTTTACA CTTTATGCTT CCGGCTCGTA TAATGTGTGG AATTGTGAGC
     4741 GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGGATTCAC TGGAACTCTA
     4801 GACCAAAGAG AGGACACAAT GCAGGGTTCT GTGACAGAGT TTCTAAAACC GCGCCTGGTT
     4861 GATATCGAGC AAGTGAGTTC GACGCACGCC AAGGTGACCC TTGAGCCTTT AGAGCGTGGC
     4921 TTTGGCCATA CTCTGGGTAA CGCACTGCGC CGTATTCTGC TCTCATCGAT GCCGGGTTGC
     4981 GCGGTGACCG AGGTTGAGAT TGATGGTGTA CTACATGAGT ACAGCACCAA AGAAGGCGTT
     5041 CAGGAAGATA TCCTGGAAAT CCTGCTCAAC CTGAAAGGGC TGGCGGTGAG AGTTCAGGGC
     5101 AAAGATGAAG TTATTCTTAC CTTGAATAAA TCTGGCATTG GCCCTGTGAC TGCAGCCGAT
     5161 ATCACCCACG ACGGTGATGT CGAAATCGTC AAGCCGCAGC ACGTGATCTG CCACCTGACC
     5221 GATGAGAACG CGTCTATTAG CATGCGTATC AAAGTTCAGC GCGGTCGTGG TTATGTGCCG
     5281 GCTTCTACCC GAATTCATTC GGAAGAAGAT GAGCGCCCAA TCGGCCGTCT GCTGGTCGAC
     5341 GCATGCTACA GCCCTGTGGA GCGTATTGCC TACAATGTTG AAGCAGCGCG TGTAGAACAG
     5401 CGTACCGACC TGGACAAGCT GGTCATCGAA ATGGAAACCA ACGGCACAAT CGATCCTGAA
     5461 GAGGCGATTC GTCGTGCGGC AACCATTCTG GCTGAACAAC TGGAAGCTTT CGTTGACTTA
     5521 CGTGATGTAC GTCAGCCTGA AGTGAAAGAA GAGAAACCAG AGGCGGCCGC AGCAAGCACC
     5581 GAACGCAAAT GGGCAGAACT GGCACGTCGT ATTCGTGGTG CAGGCGGTGT TACCCTCAAT
     5641 GGTTTTGGTT GAGGATCCTC TACGCCGGAC GCACTCGGTT CCAAAAAAAA AAAAAAAGAC
     5701 GCTGAAAAGC GTCTTTTTTT TTTTTGGTCC CCACTGGAGC ACCTCAAAAA CACCATCATA
     5761 CACTAAATCA GTAAGTT
//
