LOCUS       pSIBR-Int4-Cas9-NT      7580 bp ds-DNA     circular     26-FEB-2025
DEFINITION  .
KEYWORDS    "creator:Enrico Orsi"
COMMENT     Imported using the Genbank importer. File name:
            4_psibr-cas9_int4_nt_ev.gb Imported using the Genbank importer.
            File name: psibr-cas9_int4_nt_ev.gb Imported using the Genbank
            importer. File name: psibr-cas9_int3_nt.gb
FEATURES             Location/Qualifiers
     CDS             1..4101
                     /label="Cas9"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 locus_tag: note: generates RNA-guided double strand breaks in DNA product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system protein_id: transl_table: 1 translation: DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    1..4104
                     /label="Cas9"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             1..4104
                     /label="cas)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD*"
     misc_feature    4173..4194
                     /label="B1002 terminator"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="label: B1002 terminator"
     promoter        4201..4231
                     /label="lacUV5 promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="label: lacUV5 promoter"
     misc_feature    4232..4266
                     /label="NT sequence"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_RNA        4267..4342
                     /label="gRNA scaffold"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="note: guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system"
     terminator      4498..4519
                     /label="B1002 terminator"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="label: B1002 terminator"
     misc_feature    4627..5442
                     /label="Kan Neo"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="label: Kan Neo"
     misc_feature    complement(5516..6175)
                     /label="'rep"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="label: 'rep"
     misc_feature    complement(6176..6947)
                     /label="Ori pBBR1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="label: Ori pBBR1"
     misc_feature    7192..7221
                     /label="lcaUV5 promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="label: lcaUV5 promoter"
     misc_feature    7293..7301
                     /label="Int4 5' exon"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="label: Int4 5' exon"
     misc_feature    7302..7577
                     /label="Int 4"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="label: Int 4"
     misc_feature    7388..7417
                     /label="Theophylline"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: Theophylline"
     misc_feature    7578..7580
                     /label="Int4 3' exon"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="label: Int4 3' exon"
ORIGIN
        1 GACAAAAAGT ACTCGATTGG GCTCGACATC GGGACCAACT CCGTTGGGTG GGCTGTTATC
       61 ACCGACGAGT ACAAAGTCCC TTCGAAGAAA TTTAAAGTCT TGGGGAACAC CGATCGTCAT
      121 AGCATCAAGA AGAACCTTAT TGGAGCGCTT CTCTTCGATA GCGGGGAAAC CGCTGAGGCT
      181 ACCCGGTTGA AGCGAACCGC GCGGAGGAGG TACACCCGGC GAAAAAACCG GATCTGCTAC
      241 CTTCAGGAAA TCTTCTCGAA CGAAATGGCT AAGGTTGACG ACAGCTTTTT CCACCGTCTT
      301 GAGGAATCGT TCCTTGTTGA GGAGGATAAA AAACACGAGC GGCACCCCAT CTTCGGGAAC
      361 ATTGTTGACG AGGTCGCGTA CCACGAAAAG TACCCCACCA TCTACCACTT GCGTAAGAAG
      421 CTTGTTGACT CGACCGACAA GGCTGACCTT CGTCTCATCT ACCTTGCACT CGCTCACATG
      481 ATCAAATTCC GGGGGCACTT CCTTATCGAA GGGGACCTCA ACCCCGACAA CAGCGACGTT
      541 GATAAGCTTT TCATCCAGCT TGTTCAGACG TACAACCAGC TCTTCGAGGA GAACCCCATC
      601 AACGCTAGCG GGGTTGACGC GAAGGCTATC CTTTCGGCTC GTCTTAGCAA GTCGAGGCGT
      661 CTCGAGAACT TGATCGCGCA GTTGCCTGGG GAAAAAAAGA ACGGGCTCTT CGGAAACTTG
      721 ATCGCGCTTT CGCTTGGGCT TACGCCCAAC TTCAAGTCGA ACTTCGACCT TGCTGAGGAC
      781 GCGAAGCTCC AGCTTTCGAA GGACACCTAC GACGACGACC TCGACAACCT CCTTGCTCAG
      841 ATCGGGGACC AGTACGCGGA CCTTTTCCTT GCTGCGAAAA ACCTCTCGGA CGCGATCCTC
      901 CTTTCGGACA TCCTTAGGGT TAACACCGAG ATTACCAAAG CGCCTCTTTC GGCGTCGATG
      961 ATCAAGCGTT ACGACGAGCA CCACCAGGAT CTTACCCTTC TCAAGGCGCT CGTCCGTCAG
     1021 CAGCTTCCCG AGAAATACAA GGAGATCTTC TTCGACCAGT CGAAGAACGG GTACGCTGGG
     1081 TACATCGACG GAGGGGCGTC CCAGGAGGAG TTCTACAAGT TCATCAAGCC CATCCTCGAG
     1141 AAGATGGACG GGACCGAAGA GCTCCTTGTT AAGCTTAACC GGGAGGACCT TTTGCGTAAA
     1201 CAGCGAACGT TCGATAACGG GTCGATCCCT CACCAGATCC ATCTTGGGGA ATTGCACGCG
     1261 ATCCTTAGGA GGCAGGAGGA TTTCTACCCC TTCCTCAAGG ATAACCGGGA AAAAATCGAG
     1321 AAGATCCTTA CCTTCCGTAT CCCCTACTAC GTCGGGCCCC TTGCTCGGGG GAACAGCCGG
     1381 TTCGCTTGGA TGACCCGAAA ATCGGAGGAG ACCATCACGC CCTGGAACTT CGAGGAGGTC
     1441 GTTGACAAGG GGGCGTCGGC GCAGTCGTTC ATCGAGCGTA TGACCAACTT CGACAAGAAC
     1501 CTTCCCAACG AGAAGGTTCT TCCCAAGCAC AGCCTTCTTT ACGAATACTT CACGGTCTAC
     1561 AACGAGCTTA CCAAAGTTAA GTACGTCACC GAGGGGATGC GTAAGCCCGC TTTCCTTTCG
     1621 GGGGAGCAGA AAAAGGCAAT CGTCGACCTC TTGTTTAAGA CCAACCGTAA GGTTACGGTC
     1681 AAACAGCTCA AGGAGGACTA CTTTAAGAAG ATTGAGTGCT TCGACAGCGT CGAGATCTCG
     1741 GGGGTCGAGG ACAGGTTCAA CGCGTCGCTC GGGACGTACC ACGACCTTCT TAAGATCATC
     1801 AAGGACAAGG ACTTCCTTGA CAACGAGGAG AACGAGGACA TCCTCGAAGA CATCGTCCTC
     1861 ACCCTTACGC TCTTCGAGGA CAGGGAAATG ATCGAAGAGA GGCTTAAGAC CTACGCGCAT
     1921 TTGTTCGACG ACAAAGTTAT GAAGCAGCTT AAGCGGCGTC GGTACACCGG GTGGGGGCGG
     1981 CTTTCGCGTA AGCTTATCAA CGGGATCAGG GACAAACAGT CGGGGAAGAC CATTCTCGAC
     2041 TTCCTTAAGT CGGACGGGTT CGCAAACCGT AACTTCATGC AGTTGATCCA CGACGACAGC
     2101 CTTACCTTCA AGGAGGATAT CCAGAAGGCT CAGGTTTCGG GGCAGGGGGA CAGCCTCCAC
     2161 GAGCACATCG CTAACCTCGC GGGGTCCCCC GCGATCAAGA AGGGGATCCT CCAGACCGTT
     2221 AAGGTCGTCG ACGAGCTTGT TAAGGTTATG GGACGACACA AACCCGAGAA CATCGTCATC
     2281 GAGATGGCTC GGGAGAACCA GACCACCCAG AAAGGGCAGA AGAACTCCCG TGAACGGATG
     2341 AAGCGTATCG AGGAGGGGAT CAAGGAGCTC GGGAGCCAGA TCCTTAAGGA ACACCCCGTC
     2401 GAGAACACCC AGCTTCAGAA CGAGAAATTG TACTTGTACT ACTTGCAGAA CGGGAGGGAT
     2461 ATGTACGTTG ATCAGGAGCT CGACATCAAC CGGCTCAGCG ACTACGACGT TGACCATATC
     2521 GTCCCCCAGA GCTTTCTTAA GGATGACTCG ATTGATAACA AAGTTCTCAC GCGGTCGGAC
     2581 AAGAACCGGG GGAAGTCCGA CAACGTCCCC AGCGAGGAGG TTGTTAAGAA AATGAAGAAC
     2641 TACTGGAGGC AGCTTCTTAA CGCAAAACTC ATCACCCAGC GGAAATTCGA CAACCTCACG
     2701 AAGGCGGAGC GGGGGGGGCT TAGCGAGCTT GACAAGGCGG GGTTCATCAA GCGTCAGCTT
     2761 GTCGAGACCC GTCAGATCAC CAAACACGTT GCTCAGATCC TTGACAGCCG TATGAACACC
     2821 AAGTACGACG AGAACGACAA GCTTATCCGT GAAGTCAAGG TTATCACGCT CAAGTCGAAG
     2881 CTCGTCTCGG ATTTTCGTAA GGACTTTCAG TTTTACAAGG TTCGGGAAAT CAACAACTAC
     2941 CACCACGCAC ACGACGCTTA CCTTAACGCA GTTGTCGGGA CCGCGCTTAT CAAAAAGTAC
     3001 CCCAAGCTTG AGTCCGAATT CGTTTACGGG GACTACAAGG TCTACGACGT CCGGAAGATG
     3061 ATCGCGAAAT CGGAACAGGA GATTGGGAAG GCTACGGCTA AGTACTTTTT CTACTCGAAC
     3121 ATCATGAACT TTTTTAAGAC CGAGATCACC CTTGCTAACG GGGAAATCCG TAAGCGTCCC
     3181 CTTATCGAGA CCAACGGAGA GACCGGGGAG ATCGTTTGGG ACAAGGGACG TGACTTCGCA
     3241 ACCGTTCGTA AGGTTCTTTC CATGCCTCAG GTTAACATCG TTAAAAAGAC CGAGGTTCAG
     3301 ACCGGGGGGT TTTCCAAAGA ATCGATCCTC CCCAAGAGGA ACTCCGATAA ACTTATCGCG
     3361 CGGAAGAAGG ATTGGGACCC CAAGAAGTAC GGGGGGTTCG ACAGCCCCAC GGTTGCGTAC
     3421 TCGGTTCTTG TTGTCGCGAA AGTTGAGAAG GGAAAGTCCA AAAAACTCAA GTCCGTCAAG
     3481 GAACTCCTTG GAATCACCAT CATGGAGAGG AGCTCCTTCG AGAAGAACCC TATCGATTTC
     3541 CTCGAGGCGA AGGGGTACAA AGAGGTCAAG AAGGATCTCA TCATCAAGCT TCCCAAGTAC
     3601 AGCCTTTTCG AACTCGAGAA CGGGCGGAAG CGAATGTTGG CGAGCGCAGG GGAGCTCCAG
     3661 AAGGGGAACG AATTGGCGTT GCCCTCCAAG TACGTTAACT TCCTCTACCT CGCGAGCCAC
     3721 TACGAGAAAC TTAAAGGGAG CCCCGAGGAC AACGAGCAGA AGCAGCTTTT CGTTGAACAG
     3781 CACAAACACT ACCTCGACGA AATCATCGAA CAGATCAGCG AGTTCTCGAA ACGGGTCATC
     3841 CTCGCTGACG CAAACCTCGA CAAGGTCCTT AGCGCTTACA ACAAGCACAG GGATAAGCCC
     3901 ATTCGGGAGC AGGCTGAGAA CATCATCCAC CTCTTCACGC TTACGAACCT TGGGGCGCCT
     3961 GCGGCGTTCA AGTACTTCGA CACCACCATC GACCGGAAGC GTTACACGTC GACCAAGGAG
     4021 GTCCTCGACG CAACCCTTAT CCACCAGTCC ATCACCGGGC TTTACGAGAC CCGTATCGAC
     4081 CTTAGCCAGC TTGGGGGGGA TTAGCACCAT CACCATCACC ATTAGCGGCC GCATAATGAT
     4141 GTGTTATCAT TGATGCGAGG TCGCCTATAC CTCCCCGCTT CGGCGGGGTT TTTTCCCGGG
     4201 TTTACACTTT ATGCTTCCGG CTCGTATAAT GGATGGCAGG TGCCCCAACC CTACCCACCT
     4261 GCTAGAGTTT TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT ATCAACTTGA
     4321 AAAAGTGGCA CCGAGTCGGT GCTTTgatcc ggtggatgac cttttgaatg acctttaata
     4381 gattatatta ctaattaatt ggggacccta gaggtcccct tttttatttt aaaaattttt
     4441 tcacaaaacg gtttacaagc ataaagcttg ctcaatcaat caccGtcgac ctcGTCTCCC
     4501 CGCTTCGGCG GGGTTTTTTC CTAGGTTGTG TCTCAAAATC TCTGATGTTA CATTGCACAA
     4561 GATAAAAATA TATCATCATG AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG
     4621 GGTGTTATGA GCCATATTCA GCGTGAAACG AGCTGTAGCC GTCCGCGTCT GAACAGCAAC
     4681 ATGGATGCGG ATCTGTATGG CTATAAATGG GCGCGTGATA ACGTGGGTCA GAGCGGCGCG
     4741 ACCATTTATC GTCTGTATGG CAAACCGGAT GCGCCGGAAC TGTTTCTGAA ACATGGCAAA
     4801 GGCAGCGTGG CGAACGATGT GACCGATGAA ATGGTGCGTC TGAACTGGCT GACCGAATTT
     4861 ATGCCGCTGC CGACCATTAA ACATTTTATT CGCACCCCGG ATGATGCGTG GCTGCTGACC
     4921 ACCGCGATTC CGGGCAAAAC CGCGTTTCAG GTGCTGGAAG AATATCCGGA TAGCGGCGAA
     4981 AACATTGTGG ATGCGCTGGC CGTGTTTCTG CGTCGTCTGC ATAGCATTCC GGTGTGCAAC
     5041 TGCCCGTTTA ACAGCGATCG TGTGTTTCGT CTGGCCCAGG CGCAGAGCCG TATGAACAAC
     5101 GGCCTGGTGG ATGCGAGCGA TTTTGATGAT GAACGTAACG GCTGGCCGGT GGAACAGGTG
     5161 TGGAAAGAAA TGCATAAACT GCTGCCGTTT AGCCCGGATA GCGTGGTGAC CCACGGCGAT
     5221 TTTAGCCTGG ATAACCTGAT TTTCGATGAA GGCAAACTGA TTGGCTGCAT TGATGTGGGC
     5281 CGTGTGGGCA TTGCGGATCG TTATCAGGAT CTGGCCATTC TGTGGAACTG CCTGGGCGAA
     5341 TTTAGCCCGA GCCTGCAAAA ACGTCTGTTT CAGAAATATG GCATTGATAA TCCGGATATG
     5401 AACAAACTGC AATTTCATCT GATGCTGGAT GAATTTTTCT AATAATTAAT TGGACCGCGG
     5461 TCCGAGAGAC GCATGGCAGT ATCTCAGACT GTGTATCGGC ACGTCTCACT ACTTACCGGC
     5521 GCGGCAGCGT TACCCGTGTC GGCGGCTCCA ACGGCTCGCC ATCGTCCAGA AAACACGGCT
     5581 CATCGGGCAT CGGCAGGCGC TGCTGCCCGC GCCGTTCCCA TTCCTCCGTT TCGGTCAAGG
     5641 CTGGCAGGTC TGGTTCCATG CCCGGAATGC CGGGCTGGCT GGGCGGCTCC TCGCCGGGGC
     5701 CGGTCGGTAG TTGCTGCTCG CCCGGATACA GGGTCGGGAT GCGGCGCAGG TCGCCATGCC
     5761 CCAACAGCGA TTCGTCCTGG TCGTCGTGAT CAACCACCAC GGCGGCACTG AACACCGACA
     5821 GGCGCAACTG GTCGCGGGGC TGGCCCCACG CCACGCGGTC ATTGACCACG TAGGCCGACA
     5881 CGGTGCCGGG GCCGTTGAGC TTCACGACGG AGATCCAGCG CTCGGCCACC AAGTCCTTGA
     5941 CTGCGTATTG GACCGTCCGC AAAGAACGTC CGATGAGCTT GGAAAGTGTT TTCTGGCTGA
     6001 CCACCACGGC GTTCTGGTGG CCCATCTGCG CCACGAGGTG ATGCAGCAGC ATTGCCGCCG
     6061 TGGGTTTCCT CGCAATAAGC CCGGCCCACG CCTCATGCGC TTTGCGTTCC GTTTGCACCC
     6121 AGTGACCGGG CTTGTTCTTG GCTTGAATGC CGATTTCTCT GGACTGCGTG GCCATGCTTA
     6181 TCTCCATGCG GTAGGGGTGC CGCACGGTTG CGGCACCATG CGCAATCAGC TGCAACTTTT
     6241 CGGCAGCGCG ACAACAATTA TGCGTTGCGT AAAAGTGGCA GTCAATTACA GATTTTCTTT
     6301 AACCTACGCA ATGAGCTATT GCGGGGGGTG CCGCAATGAG CTGTTGCGTA CCCCCCTTTT
     6361 TTAAGTTGTT GATTTTTAAG TCTTTCGCAT TTCGCCCTAT ATCTAGTTCT TTGGTGCCCA
     6421 AAGAAGGGCA CCCCTGCGGG GTTCCCCCAC GCCTTCGGCG CGGCTCCCCC TCCGGCAAAA
     6481 AGTGGCCCCT CCGGGGCTTG TTGATCGACT GCGCGGCCTT CGGCCTTGCC CAAGGTGGCG
     6541 CTGCCCCCTT GGAACCCCCG CACTCGCCGC CGTGAGGCTC GGGGGGCAGG CGGGCGGGCT
     6601 TCGCCCTTCG ACTGCCCCCA CTCGCATAGG CTTGGGTCGT TCCAGGCGCG TCAAGGCCAA
     6661 GCCGCTGCGC GGTCGCTGCG CGAGCCTTGA CCCGCCTTCC ACTTGGTGTC CAACCGGCAA
     6721 GCGAAGCGCG CAGGCCGCAG GCCGGAGGCT TTTCCCCAGA GAAAATTAAA AAAATTGATG
     6781 GGGCAAGGCC GCAGGCCGCG CAGTTGGAGC CGGTGGGTAT GTGGTCGAAG GCTGGGTAGC
     6841 CGGTGGGCAA TCCCTGTGGT CAAGCTCGTG GGCAGGCGCA GCCTGTCCAT CAGCTTGTCC
     6901 AGCAGGGTTG TCCACGGGCC GAGCGAAGCG AGCCAGCCGG TGGCCGCTCG CGGCCATCGT
     6961 CCACATATCC ACGGGCTGGC AAGGGAGCGC AGCGACCGCG CAGGGCGAAG CCCGGAGAGC
     7021 AAGCCCGTAG GGGGGGCGCG CCCAGCTGTC TAGGGCGGCG GATTTGTCCT ACTCAGGAGA
     7081 GCGTTCACCG ACAAACAACA GATAAAACGA AAGGCCCAGT CTTTCGACTG AGCCTTTCGT
     7141 TTTATTTGAT GCCTTTAATT AAAGCGGATA ACAATTTCAC ACAGGAGGCC GTTTACACTT
     7201 TATGCTTCCG GCTCGTATAA TGTGTGGGGA AACCACAACG GTTTCCCTCT AGAAATAATT
     7261 TTGTTTAACT ATAAGAAGGA GATATACATA TGTCCTCTGG TTAATTGAGG CCTGAGTATA
     7321 AGGTGACTTA TACTTGTAAT CTATCTAAAC GGGGAACCTC TCTAGTAGAC AATCCCGTGC
     7381 TAAATTGATA CCAGCATCGT CTTGATGCCC TTGGCAGCAT AAATGCCTAA CGACTATCCC
     7441 TTTGGGGAGT AGGGTCAAGT GACTCGAAAC GATAGACAAC TTGCTTTAAC AAGTTGGAGA
     7501 TATAGTCTGC TCTGCATGGT GACATGCAGC TGGATATAAT TCCGGGGTAA GATTAACGAC
     7561 CTTATCTGAA CATAATGCTA
//