LOCUS       STK160_pSTK-0A-Pmanitol 2255 bp ds-DNA     circular     29-JAN-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    330..349
                     /label="S077 For"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    471..485
                     /label="overhang"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    486..504
                     /label="For"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     promoter        486..656
                     /label="Pmanitol short"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(632..656)
                     /label="Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    657..671
                     /label="overhang"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(842..862)
                     /label="S078 Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     rep_origin      complement(930..1518)
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(1816..220)
                     /label="Chloramphenicol amino transferase CDS"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
ORIGIN
        1 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
       61 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
      121 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
      181 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
      241 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
      301 tggaacctct tacgtgcccg atcaactcga gtgccacctg acgtctaaga aaccattatt
      361 atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa
      421 tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga ctgaGGTCTC
      481 AAGAGgcgga taacaatttc acacaggagg ccgatcgcgt cctgaaacca ggagcctttt
      541 tatttttaaa aaattgtcac agtcatgtgc caaagtcctc tttactttca attgtatagg
      601 gactgtaagc gttttaacat agagtcaaag ggaagcatca ttctctgaac atatttACAT
      661 AGAGACCcct cctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct
      721 ttaaaaccga aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc
      781 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      841 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
      901 aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat
      961 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1021 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1081 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1141 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1201 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1261 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1321 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1381 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1441 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1501 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1561 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1621 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1681 tctgacagct cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt
     1741 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
     1801 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     1861 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     1921 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     1981 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     2041 cacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     2101 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     2161 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     2221 ccatatcacc agctcaccgt ctttcattgc catac
//