LOCUS       STK205_pSTK0-B-RBS2     2107 bp ds-DNA     circular     29-JAN-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    492..506
                     /label="R2-RBS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    507..518
                     /label="Scar"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      complement(788..1376)
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(1674..226)
                     /label="Chloramphenicol amino transferase CDS"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
ORIGIN
        1 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
       61 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
      121 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
      181 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
      241 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
      301 gaaagttgga acctcttacg tgcccgatca actcgagtgc cacctgacgt ctaagaaacc
      361 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
      421 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagactga
      481 GGTCTCAACA Tggtggaaag gaggtgatga catgTATGTG AGACCCCTCc tgcagtccgg
      541 caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc
      601 gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      661 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      721 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      781 tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      841 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      901 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      961 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1021 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1081 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1141 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1201 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1261 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1321 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1381 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1441 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1501 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctcg aggcttggat
     1561 tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
     1621 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
     1681 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     1741 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     1801 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     1861 aaatcaaaac tggtgaaact cacccaggga ttggctgaca cgaaaaacat attctcaata
     1921 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     1981 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     2041 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     2101 ttcattg
//