LOCUS       STK053_pSTK-0C-GFPmut3b 2798 bp ds-DNA     circular     28-JAN-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     source          2..6
                     /label="source"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    7..7
                     /label="universal suffix for all parts"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     source          7..2055
                     /label="source"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     terminator      8..65
                     /label="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined."
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      complement(260..848)
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     terminator      1031..1125
                     /label="transcription terminator from phage lambda"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(1146..1805)
                     /label="cat CDS"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     terminator      1989..2032
                     /label="putative bacterial transcription terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2035..2050
                     /label="BioBrick prefix for parts that do not start with """"ATG"""""
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2066..2087
                     /label="pSTK0-Cy-GFP Fw"
                     /note="sequence: GAAGACAACTGAGGTCTCACGAAatgcgtaaaggagaagaact"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2068..2087
                     /label="pSTK0-Cz-GFP Fw"
                     /note="sequence: GAAGACAACTGAGGTCTCAGGTAatgcgtaaaggagaagaact"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             2068..2784
                     /label="GFPmut3b- Aequoria victoria green fluorescent protein"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             2068..2784
                     /label="Translation 2068-2784"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK*"
     primer          complement(2761..4)
                     /label="pSTK0-Cz-GFP Rv"
                     /note="sequence: GAAGACAAGAGGGGTCTCATGTCttatttgtatagttcatccatgcc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2761..2781)
                     /label="pSTK0-Cy-GFP Rv"
                     /note="sequence: GAAGACAAGAGGGGTCTCATACCtttgtatagttcatccatgcc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 cctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga
       61 aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc
      121 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      181 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      241 aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc
      301 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      361 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      421 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      481 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      541 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      601 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      661 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      721 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      781 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      841 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      901 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
      961 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct
     1021 cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     1081 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
     1141 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     1201 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     1261 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     1321 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga cacgaaaaac
     1381 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     1441 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     1501 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     1561 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
     1621 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     1681 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     1741 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     1801 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     1861 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc
     1921 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     1981 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc
     2041 gcggccgctt ctagaCTGAG GTCTCATatg cgtaaaggag aagaactttt cactggagtt
     2101 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
     2161 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
     2221 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt
     2281 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt
     2341 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
     2401 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
     2461 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
     2521 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
     2581 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     2641 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
     2701 aacgaaaaga gagatcacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     2761 ggcatggatg aactatacaa ataaGACATG AGACCcct
//