LOCUS       HP17_KO7_sfgfp::P8      9946 bp ds-DNA     circular     27-FEB-2025
DEFINITION  .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name:
            addgene-plasmid-120346-sequence-250005.gbk Helper plasmid for
            production of phagemid ssDNA, based on M13KO7.
FEATURES             Location/Qualifiers
     source          1..9946
                     /label="source"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     686..705
                     /label="Kan-R"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     770..789
                     /label="pENTR-R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(1136..2086)
                     /label="Rep101"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      2134..2355
                     /label="pSC101 ori"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     terminator      2847..2941
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     terminator      3097..3126
                     /label="T3Te terminator"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3275..3304
                     /label="Prom gene II"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             3337..4569
                     /label="M13 gene II"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    4137..4166
                     /label="Prom gene X (M13110)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    4544..4573
                     /label="Prom gene V"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             4581..4844
                     /label="M13 gene V"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             4846..4947
                     /label="M13 gene VII"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4910..4939
                     /label="Prom gene VIII"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             4944..5038
                     /label="M13 gene IX"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     gene            5039..5752
                     /label="sfGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    5759..5783
                     /label="Prom gene III"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     terminator      5762..5810
                     /label="fd terminator"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             5815..7089
                     /label="M13 gene III"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    6971..7000
                     /label="Prom gene VI"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             7092..7430
                     /label="M13 gene VI"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    7339..7368
                     /label="Prom gene 1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             7432..8478
                     /label="M13 gene I"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    8310..8339
                     /label="Prom gene IV"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             8456..9736
                     /label="M13 gene IV"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     terminator      9779..9810
                     /label="tonB terminator"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(9909..778)
                     /label="KanR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
       61 aaaactcacc gaggcagttc caaagaatgg caaggtcctg gtaacggtct gcgattccga
      121 cccgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
      181 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caagagcttg tgcatttctt
      241 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
      301 aaccgttatt catgcgtgat tgcgcctgag caagacgaaa tacacgatcg ctgttaaaag
      361 gacaattaca aacaggaatc gaatgtaacc ggcgcaggaa cacggccagc gcatcaacaa
      421 tattttcacc tgaatcagga tattcttcta atacctggaa ggctgttttc ccaggaatcg
      481 cggtggtgag taaccacgca tcatcaggag tacggataaa atgcttgatg gtcgggagag
      541 gcataaactc cgtcagccag ttgagacgga ccatctcatc tgtaacatca ttggcaacgc
      601 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aagcgataga
      661 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcgt
      721 ccatgttgga gtttaagcgc ggacgggagc aagacgtttc ccgttgaata tggctcataa
      781 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt
      841 tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttgt tgaataaatc
      901 gaacttttgc tgagttgaag gatcagctct agtagttaca ttgtcgatct gttcatggtg
      961 aacagctttg aatgcaccaa aaactcgtaa aagctctgat gtatctatct tttttacacc
     1021 gttttcatct gtgcatatgg acagttttcc ctttgatatg taacggtgaa cagttgttct
     1081 acttttgttt gttagtcttg atgcttcact gatagataca agagccataa gaacctcaga
     1141 tccttccgta tttagccagt atgttctcta gtgtggttcg ttgtttttgc gtgagccatg
     1201 agaacgaacc attgagatca tacttacttt gcatgtcact caaaaatttt gcctcaaaac
     1261 tggtgagctg aatttttgca gttaaagcat cgtgtagtgt ttttcttagt ccgttatgta
     1321 ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc attcattttt atctggttgt
     1381 tctcaagttc ggttacgaga tccatttgtc tatctagttc aacttggaaa atcaacgtat
     1441 cagtcgggcg gcctcgctta tcaaccacca atttcatatt gctgtaagtg tttaaatctt
     1501 tacttattgg tttcaaaacc cattggttaa gccttttaaa ctcatggtag ttattttcaa
     1561 gcattaacat gaacttaaat tcatcaaggc taatctctat atttgccttg tgagttttct
     1621 tttgtgttag ttcttttaat aaccactcat aaatcctcat agagtatttg ttttcaaaag
     1681 acttaacatg ttccagatta tattttatga atttttttaa ctggaaaaga taaggcaata
     1741 tctcttcact aaaaactaat tctaattttt cgcttgagaa cttggcatag tttgtccact
     1801 ggaaaatccc aaagccttta accaaaggat tcctgatttc cacagttctc gtcatcagct
     1861 ctctggttgc tttagctaat acaccataag cattttccct actgatgttc atcatctgag
     1921 cgtattggtt ataagtgaac gataccgtcc gttctttcct tgtagggttt tcaatcgtgg
     1981 ggttgagtag tgccacacag cataaaatta gcttggtttc atgctccgtt aagtcatagc
     2041 gactaatcgc tagttcattt gctttgaaaa caactaattc agacatacat ctcaattggt
     2101 ctaggtgatt ttaatcacta taccaattga gatgggctag tcaatgataa ttacatgtcc
     2161 ttttcctttg agttgtgggt atctgtaaat tctgctagac ctttgctgga aaacttgtaa
     2221 attctgctag accctctgta aattccgcta gacctttgtg tgtttttttg tttatattca
     2281 agtggttata atttatagaa taaagaaaga ataaaaaaag ataaaaagaa tagatcccag
     2341 ccctgtgtat aactcactac tttagtcagt tccgcagtat tacaaaagga tgtcgcaaac
     2401 gctgtttgct cctctacaaa acagacctta aaaccctaaa ggcttaagta gcaccctcgc
     2461 aagctcgggc aaatcgctga atattccttt tgtctccgac catcaggcac ctgagtcgct
     2521 gtctttttcg tgacattcag ttcgctgcgc tcacggctct ggcagtgaat gggggtaaat
     2581 ggcactacag gcgcctttta tggattcatg caaggaaact acccataata caagaaaagc
     2641 ccgtcacggg cttctcaggg cgttttatgg cgggtctgct atgtggtgct atctgacttt
     2701 ttgctgttca gcagttcctg ccctctgatt ttccagtctg accacttcgg attatcccgt
     2761 gacaggtcat tcagactggc taatgcaccc agtaaggcag cggtatcatc aacaggctta
     2821 cccgtcttac tgtccctagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     2881 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     2941 ccaagcgagc tcgtaaactt ggtctgacag ctctagctcc ggcaaaaaaa cgggcaaggt
     3001 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgttt gccacctgac
     3061 gtctaagaaa aggaatattc agcaatttgc ccgtgccgaa gaaaggccca cccgtgaagg
     3121 tgagccagtg agttgattgc tacgtaatta gttagttagc ccttagtgac tccagacgcg
     3181 aattattttt gatggcgttc ctattggtta aaaaatgagc tgatttaaca aaaatttaat
     3241 gcgaatttta acaaaatatt aacgtttaca atttaaatat ttgcttatac aatcttcctg
     3301 tttttggggc ttttctgatt atcaaccggg gtacatatga ttgacatgct agttttacga
     3361 ttaccgttca tcgattctct tgtttgctcc agactctcag gcaatgacct gatagccttt
     3421 gtagacctct caaaaatagc taccctctcc ggcatgaatt tatcagctag aacggttgaa
     3481 tatcatattg atggtgattt gactgtctcc ggcctttctc acccttttga atctttacct
     3541 acacattact caggcattgc atttaaaata tatgagggtt ctaaaaattt ttatccttgc
     3601 gttgaaataa aggcttctcc cgcaaaagta ttacagggtc ataatgtttt tggtacaacc
     3661 gatttagctt tatgctctga ggctttattg cttaattttg ctaattcttt gccttgcctg
     3721 tatgatttat tggatgttaa cgctactact attagtagaa ttgatgccac cttttcagct
     3781 cgcgccccaa atgaaaatat agctaaacag gttattgacc atttgcgaaa tgtatctaat
     3841 ggtcaaacta aatctactcg ttcgcagaat tgggaatcaa ctgttacatg gaatgaaact
     3901 tccagacacc gtactttagt tgcatattta aaacatgttg agctacagca ccagattcag
     3961 caattaagct ctaagccatc cgcaaaaatg acctcttatc aaaaggagca attaaaggta
     4021 ctctctaatc ctgacctgtt ggagtttgct tccggtctgg ttcgctttga agctcgaatt
     4081 aaaacgcgat atttgaagtc tttcgggctt cctcttaatc tttttgatgc aatccgcttt
     4141 gcttctgact ataatagtca gggtaaagac ctgatttttg atttatggtc attctcgttt
     4201 tctgaactgt ttaaagcatt tgagggggat tcaatgaata tttatgacga ttccgcagta
     4261 ttggacgcta tccagtctaa acattttact attaccccct ctggcaaaac ttcttttgca
     4321 aaagcctctc gctattttgg tttttatcgt cgtctggtaa acgagggtta tgatagtgtt
     4381 gctcttacta tgcctcgtaa ttccttttgg cgttatgtat ctgcattagt tgaatgtggt
     4441 attcctaaat ctcaactgat gaatctttct acctgtaata atgttgttcc gttagttcgt
     4501 tttattaacg tagatttttc ttcccaacgt cctgactggt ataatgagcc agttcttaaa
     4561 atcgcataag gtaattcaca atgattaaag ttgaaattaa accatctcaa gcccaattta
     4621 ctactcgttc tggtgtttct cgtcagggca agccttattc actgaatgag cagctttgtt
     4681 acgttgattt gggtaatgaa tatccggttc ttgtcaagat tactcttgat gaaggtcagc
     4741 cagcctatgc gcctggtctg tacaccgttc atctgtcctc tttcaaagtt ggtcagttcg
     4801 gttcccttat gattgaccgt ctgcgcctcg ttccggctaa gtaacatgga gcaggtcgcg
     4861 gatttcgaca caatttatca ggcgatgata caaatctccg ttgtactttg tttcgcgctt
     4921 ggtataatcg ctgggggtca aagatgagtg ttttagtgta ttctttcgcc tctttcgttt
     4981 taggttggtg ccttcgtagt ggcattacgt attttacccg tttaatggaa acttcctcAT
     5041 GAGAAAAGGC GAAGAACTGT TTACCGGTGT GGTTCCGATT CTGGTGGAAC TGGACGGCGA
     5101 TGTTAATGGT CATAAATTCA GTGTTCGCGG CGAAGGTGAA GGCGATGCGA CGAACGGCAA
     5161 ACTGACCCTG AAATTTATCT GCACCACGGG TAAACTGCCG GTCCCGTGGC CGACGCTGGT
     5221 GACCACGCTG ACCTATGGCG TTCAATGTTT TGCGCGTTAC CCGGATCACA TGAAACAGCA
     5281 CGACTTTTTC AAATCGGCCA TGCCGGAAGG CTATGTGCAG GAACGTACGA TTAGCTTTAA
     5341 AGACGATGGT ACGTATAAAA CCCGCGCGGA AGTGAAATTC GAAGGCGATA CCCTGGTTAA
     5401 CCGTATCGAA CTGAAAGGTA TCGATTTCAA AGAAGACGGC AATATTCTGG GTCATAAACT
     5461 GGAATATAAC TTCAATTCCC ACAACGTGTA CATCACCGCG GATAAACAGA AAAACGGCAT
     5521 TAAAGCCAAT TTCAAAATCC GCCATAATGT GGAAGATGGT AGCGTTCAGC TGGCCGACCA
     5581 CTATCAGCAA AACACGCCGA TTGGTGATGG CCCGGTCCTG CTGCCGGACA ATCACTACCT
     5641 GAGTACCCAG TCCGTGCTGT CAAAAGATCC GAACGAAAAA CGTGACCACA TGGTCCTGCT
     5701 GGAATTTGTG ACGGCTGCGG GTATCACCCA CGGCATGGAC GAACTGTATA AATAATAAta
     5761 aaccgataca attaaaggct ccttttggag cctttttttt tggagatttt caacgtgaaa
     5821 aaattattat tcgcaattcc tttagttgtt cctttctatt ctcactccgc tgaaactgtt
     5881 gaaagttgtt tagcaaaacc ccatacagaa aattcattta ctaacgtctg gaaagacgac
     5941 aaaactttag atcgttacgc taactatgag ggctgtctgt ggaatgctac aggcgttgta
     6001 gtttgtactg gtgacgaaac tcagtgttac ggtacatggg ttcctattgg gcttgctatc
     6061 cctgaaaatg agggtggtgg ctctgagggt ggcggttctg agggtggcgg ttctgagggt
     6121 ggcggtacta aacctcctga gtacggtgat acacctattc cgggctatac ttatatcaac
     6181 cctctcgacg gcacttatcc gcctggtact gagcaaaacc ccgctaatcc taatccttct
     6241 cttgaggagt ctcagcctct taatactttc atgtttcaga ataataggtt ccgaaatagg
     6301 cagggggcat taactgttta tacgggcact gttactcaag gcactgaccc cgttaaaact
     6361 tattaccagt acactcctgt atcatcaaaa gccatgtatg acgcttactg gaacggtaaa
     6421 ttcagagact gcgctttcca ttctggcttt aatgaggatc cattcgtttg tgaatatcaa
     6481 ggccaatcgt ctgacctgcc tcaacctcct gtcaatgctg gcggcggctc tggtggtggt
     6541 tctggtggcg gctctgaggg tggtggctct gagggtggcg gttctgaggg tggcggctct
     6601 gagggaggcg gttccggtgg tggctctggt tccggtgatt ttgattatga aaagatggca
     6661 aacgctaata agggggctat gaccgaaaat gccgatgaaa acgcgctaca gtctgacgct
     6721 aaaggcaaac ttgattctgt cgctactgat tacggtgctg ctatcgatgg tttcattggt
     6781 gacgtttccg gccttgctaa tggtaatggt gctactggtg attttgctgg ctctaattcc
     6841 caaatggctc aagtcggtga cggtgataat tcacctttaa tgaataattt ccgtcaatat
     6901 ttaccttccc tccctcaatc ggttgaatgt cgcccttttg tctttggcgc tggtaaacca
     6961 tatgaatttt ctattgattg tgacaaaata aacttattcc gtggtgtctt tgcgtttctt
     7021 ttatatgttg ccacctttat gtatgtattt tctacgtttg ctaacatact gcgtaataag
     7081 gagtcttaat catgccagtt cttttgggta ttccgttatt attgcgtttc ctcggtttcc
     7141 ttctggtaac tttgttcggc tatctgctta cttttcttaa aaagggcttc ggtaagatag
     7201 ctattgctat ttcattgttt cttgctctta ttattgggct taactcaatt cttgtgggtt
     7261 atctctctga tattagcgct caattaccct ctgactttgt tcagggtgtt cagttaattc
     7321 tcccgtctaa tgcgcttccc tgtttttatg ttattctctc tgtaaaggct gctattttca
     7381 tttttgacgt taaacaaaaa atcgtttctt atttggattg ggataaataa tatggctgtt
     7441 tattttgtaa ctggcaaatt aggctctgga aagacgctcg ttagcgttgg taagattcag
     7501 gataaaattg tagctgggtg caaaatagca actaatcttg atttaaggct tcaaaacctc
     7561 ccgcaagtcg ggaggttcgc taaaacgcct cgcgttctta gaataccgga taagccttct
     7621 atatctgatt tgcttgctat tgggcgcggt aatgattcct acgatgaaaa taaaaacggc
     7681 ttgcttgttc tcgatgagtg cggtacttgg tttaataccc gttcttggaa tgataaggaa
     7741 agacagccga ttattgattg gtttctacat gctcgtaaat taggatggga tattattttt
     7801 cttgttcagg acttatctat tgttgataaa caggcgcgtt ctgcattagc tgaacatgtt
     7861 gtttattgtc gtcgtctgga cagaattact ttaccttttg tcggtacttt atattctctt
     7921 attactggct cgaaaatgcc tctgcctaaa ttacatgttg gcgttgttaa atatggcgat
     7981 tctcaattaa gccctactgt tgagcgttgg ctttatactg gtaagaattt gtataacgca
     8041 tatgatacta aacaggcttt ttctagtaat tatgattccg gtgtttattc ttatttaacg
     8101 ccttatttat cacacggtcg gtatttcaaa ccattaaatt taggtcagaa gatgaaatta
     8161 actaaaatat atttgaaaaa gttttctcgc gttctttgtc ttgcgattgg atttgcatca
     8221 gcatttacat atagttatat aacccaacct aagccggagg ttaaaaaggt agtctctcag
     8281 acctatgatt ttgataaatt cactattgac tcttctcagc gtcttaatct aagctatcgc
     8341 tatgttttca aggattctaa gggaaaatta attaatagcg acgatttaca gaagcaaggt
     8401 tattcactca catatattga tttatgtact gtttccatta aaaaaggtaa ttcaaatgaa
     8461 attgttaaat gtaattaatt ttgttttctt gatgtttgtt tcatcatctt cttttgctca
     8521 ggtaattgaa atgaataatt cgcctctgcg cgattttgta acttggtatt caaagcaatc
     8581 aggcgaatcc gttattgttt ctcccgatgt aaaaggtact gttactgtat attcatctga
     8641 cgttaaacct gaaaatctac gcaatttctt tatttctgtt ttacgtgcaa ataattttga
     8701 tatggtaggt tctaaccctt ccattattca gaagtataat ccaaacaatc aggattatat
     8761 tgatgaattg ccatcatctg ataatcagga atatgatgat aattccgctc cttctggtgg
     8821 tttctttgtt ccgcaaaatg ataatgttac tcaaactttt aaaattaata acgttcgggc
     8881 aaaggattta atacgagttg tcgaattgtt tgtaaagtct aatacttcta aatcctcaaa
     8941 tgtattatct attgacggct ctaatctatt agttgttagt gctcctaaag atattttaga
     9001 taaccttcct caattccttt caactgttga tttgccaact gaccagatat tgattgaggg
     9061 tttgatattt gaggttcagc aaggtgatgc tttagatttt tcatttgctg ctggctctca
     9121 gcgtggcact gttgcaggcg gtgttaatac tgaccgcctc acctctgttt tatcttctgc
     9181 tggtggttcg ttcggtattt ttaatggcga tgttttaggg ctatcagttc gcgcattaaa
     9241 gactaatagc cattcaaaaa tattgtctgt gccacgtatt cttacgcttt caggtcagaa
     9301 gggttctatc tctgttggcc agaatgtccc ttttattact ggtcgtgtga ctggtgaatc
     9361 tgccaatgta aataatccat ttcagacgat tgagcgtcaa aatgtaggta tttccatgag
     9421 cgtttttcct gttgcaatgg ctggcggtaa tattgttctg gatattacca gcaaggccga
     9481 tagtttgagt tcttctactc aggcaagtga tgttattact aatcaaagaa gtattgctac
     9541 aacggttaat ttgcgtgatg gacagactct tttactcggt ggcctcactg attataaaaa
     9601 cacttctcag gattctggcg taccgttcct gtctaaaatc cctttaatcg gcctcctgtt
     9661 tagctcccgc tctgattcta acgaggaaag cacgttatac gtgctcgtca aagcaaccat
     9721 agtacgcgcc ctgtagcatg caggagtcac taagggttag ttagttagat tagcagaaag
     9781 tcaaaagcct ccgaccggag gcttttgact aaaacttccc ttggggttat cattggggct
     9841 cactcaaagg cggtaatcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgc
     9901 tagtattatt agaaaaactc atcgagcatc aaatgaaact gcaatt
//