LOCUS       pHK-506.5               5018 bp ds-DNA     circular     27-FEB-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    37..71
                     /label="J23115"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(117..139)
                     /label="bs-3"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    206..220
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    221..326
                     /label="sgRNA-5"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    332..346
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    380..469
                     /label="ECK120029600 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    691..725
                     /label="P(BBa_J23100)"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(786..808)
                     /label="bs-5"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    880..894
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    916..921
                     /label="RBS"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     gene            929..1648
                     /label="sfGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1672..1732
                     /label="L3S2P21 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1988..2022
                     /label="J23100"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2044..2049
                     /label="RBS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             2057..2764
                     /label="mCherry"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="label: mCherry"
     misc_feature    2942..2989
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: T7 terminator"
     gene            complement(3448..4263)
                     /label="KanR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     oriT            4373..5008
                     /label="ColA ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 GAATTCATAG GATAGATTCT GGAAACTTTA CCGTCCttta tagctagctc agcccttggt
       61 acaatgctag cAGATAGCCG TTACACAGGT GACACTTATT TCAGCCTGCG GTCCGGccaA
      121 GAGTACACTA GCTCAGTCAT CGCTGGGACG CCCGGGATCC AAGAGATTTC TACACGATTG
      181 AGCACTGTCT CAGCCTGCGG TCCGGCTGCC GTATAGGCAG AAGTGAGTCT GAGCTTAGAT
      241 GTTTcAGAGC TATGCTGGAA ACAGCATAGC AAGTTgAAAT AAGGCTAGTC CGTTATCAAC
      301 TTGAAAAAGT GGCACCGAGT CGGTGCGTTC ACTGCCGTAT AGGCAGTCGC TGGGACGCCC
      361 GAGGCTTACC TTACCATGGT TCAGCCAAAA AACTTAAGAC CGCCGGTCTT GTCCACTACC
      421 TTGCAGTAAT GCGGTGGACA GGATCGGCGG TTTTCTTTTC TCTTCTCAAT TCTTCTGACC
      481 TGTAACGAAT AATAGATAGT AAAGTAGTCT CCGATTGAGT TTTCTCTGCC GAGTCCCACC
      541 CAGTTCTGTG ATTTCAGTAA GTTGGTAATT GATACACTGT TGCGAGAACT GCTGCCTGGT
      601 AGTAGATAGG TTGTTATTGA GTAAGAAGGT AAAGTGAACG AAATCCCTGA AACTGAGACT
      661 GTAGAAAATA AGCTTCAGCC TGCGGTCCGG ttgacggcta gctcagtcct aggtacagtg
      721 ctagcTCGCT GGGACGCCCG AGATAGCCGT TACACAGGTG ACACTTATTT CAGCCTGCGG
      781 TCCGGccaAT CTAAGCTCAG ACTCACTTTC GCTGGGACGC CCGGGATCCA AGAGATTTCT
      841 ACACGATTGA GCACTGTCTC AGCCTGCGGT CCGGGTTCAC TGCCGTATAG GCAGTAATTT
      901 TGTTTAACTT TAAGAAGGAG ATATACATAT GCGTAAAGGC GAAGAACTGT TTACCGGTGT
      961 GGTTCCGATT CTGGTGGAAC TGGACGGCGA TGTTAATGGT CATAAATTCA GTGTTCGCGG
     1021 CGAAGGTGAA GGCGATGCGA CGAACGGCAA ACTGACCCTG AAATTTATCT GCACCACGGG
     1081 TAAACTGCCG GTCCCGTGGC CGACGCTGGT GACCACGCTG ACCTATGGCG TTCAATGTTT
     1141 TGCGCGTTAC CCGGATCACA TGAAACAGCA CGACTTTTTC AAATCGGCCA TGCCGGAAGG
     1201 CTATGTGCAG GAACGTACGA TTAGCTTTAA AGACGATGGT ACGTATAAAA CCCGCGCGGA
     1261 AGTGAAATTC GAAGGCGATA CCCTGGTTAA CCGTATCGAA CTGAAAGGTA TCGATTTCAA
     1321 AGAAGACGGC AATATTCTGG GTCATAAACT GGAATATAAC TTCAATTCCC ACAACGTGTA
     1381 CATCACCGCG GATAAACAGA AAAACGGCAT TAAAGCCAAT TTCAAAATCC GCCATAATGT
     1441 GGAAGATGGT AGCGTTCAGC TGGCCGACCA CTATCAGCAA AACACGCCGA TTGGTGATGG
     1501 CCCGGTCCTG CTGCCGGACA ATCACTACCT GAGTACCCAG TCCGTGCTGT CAAAAGATCC
     1561 GAACGAAAAA CGTGACCACA TGGTCCTGCT GGAATTTGTG ACGGCTGCGG GTATCACCCA
     1621 CGGCATGGAC GAACTGTATA AATAATAATC GCTGGGACGC CCGCCTGCAG GCTCGGTACC
     1681 AAATTCCAGA AAAGAGGCCT CCCGAAAGGG GGGCCTTTTT TCGTTTTGGT CCTAATAGAT
     1741 AAAGGATAGG TCTGGTAGTG TTGTTCGTTC TCGCAGGTAA ATCAATAATA CTCAGCAGTT
     1801 CCGTAGACTT TTCAGTGGGA CAGGGTAGCG ATAACAGATA GATTGTAATA AGACACAGTA
     1861 GGTGCTCGTA GTTGCGTGAA GAGAACCGCT CAGGAAATCC AGTCAGAAGT ATTGGTAATC
     1921 GTTGAAAACT CAgCTCCTAG GATGCAGTGA CTCGAGtTGT aAAACGACGG CCAgtcaatt
     1981 ggaattcttg acggctagct cagtcctagg tacagtgcta gcTAATTTTG TTTAACTTTA
     2041 AGAAGGAGAT ATACATATGG TGAGCAAGGG CGAGGAGGAT AACATGGCCA TCATCAAGGA
     2101 GTTCATGCGC TTCAAGGTGC ACATGGAGGG CTCCGTGAAC GGCCACGAGT TCGAGATCGA
     2161 GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC GCCAAGCTGA AGGTGACCAA
     2221 GGGTGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCT CAGTTCATGT ACGGCTCCAA
     2281 GGCCTACGTG AAGCACCCCG CCGACATCCC CGACTACTTG AAGCTGTCCT TCCCCGAGGG
     2341 CTTCAAGTGG GAGCGCGTGA TGAACTTCGA GGACGGCGGC GTGGTGACCG TGACCCAGGA
     2401 CTCCTCtCTG CAGGACGGCG AGTTCATCTA CAAGGTGAAG CTGCGCGGCA CCAACTTCCC
     2461 CTCCGACGGC CCCGTAATGC AGAAGAAGAC tATGGGCTGG GAGGCCTCCT CCGAGCGGAT
     2521 GTACCCCGAG GACGGCGCgC TGAAGGGCGA GATCAAGCAG AGGCTGAAGC TGAAGGACGG
     2581 CGGCCACTAC GACGCTGAaG TCAAGACCAC CTACAAGGCC AAGAAGCCCG TGCAGCTGCC
     2641 CGGCGCgTAC AACGTCAACA TCAAGTTGGA CATCACCTCC CACAACGAGG ACTACACCAT
     2701 CGTGGAACAG TACGAACGCG CCGAGGGCCG CCACTCCACC GGCGGCATGG ACGAGCTGTA
     2761 CAAGTAAact agtTgcggcc gcCTGCGCAC CACCTGCAGG ACCTCAGCAA TCGCTTGGAC
     2821 CAGCTTTCTT AAGGGTACCT ATTACAGCTG GATCCGGCGC CGCTCATTCG CTAATCGCCA
     2881 CGacgcgtAg tcgacCCGCC AATTCCCATG GGGCGCGCCT GCTGCCACCG CTGAGCAATA
     2941 ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC CAGGCATCAA
     3001 ATAAAACGAA AGGCTCAGTC GGAAGACTGG GGCGAACGAC GAGTCACTGT TGAGGATAAA
     3061 TACTTTCTCT ACTAGGCGCC TGTTACACAG GTCCTCAGCG GCGCGCCTTT GTCGGTGAAC
     3121 GCTCTCCTGA GTAGGACAAA TCCGCCGGGA GCGGATTTGA ACGTTGTGAA GCAACGGCCC
     3181 GGAGGGTGGC GGGCAGGACG CCCGCCATAA ACTGCCAGGC ATCAAACTAA GCAGAAGGCC
     3241 ATCCTGACGG ATGGCCTTTT TGCGTTTCAG ATCTACCGGT aaaccagcaa tagacataag
     3301 cggctattta acgaccctgc cctgaaccga cgacaagctg acgaccgggt ctccgcaagt
     3361 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3421 aatatgtatc cgctcatgaa ttaattctta gaaaaactca tcgagcatca aatgaaactg
     3481 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga
     3541 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat
     3601 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
     3661 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat
     3721 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc
     3781 aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc ggtcgctgtt
     3841 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc
     3901 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg
     3961 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg
     4021 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc
     4081 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg
     4141 atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc
     4201 agcatccatg ttggaattta atcgcggcct agagcaagac gtttcccgtt gaatatggct
     4261 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4321 atacatattt gaatgtattt agaaaaataa acaaataggc atgctagcgc agaaacgtcc
     4381 tagaagatgc caggaggata cttagcagag agacaataag gccggagcga agccgttttt
     4441 ccataggctc cgcccccctg acgaacatca cgaaatctga cgctcaaatc agtggtggcg
     4501 aaacccgaca ggactataaa gataccaggc gtttccccct gatggctccc tcttgcgctc
     4561 tcctgttccc gtcctgcggc gtccgtgttg tggtggaggc tttacccaaa tcaccacgtc
     4621 ccgttccgtg tagacagttc gctccaagct gggctgtgtg caagaacccc ccgttcagcc
     4681 cgactgctgc gccttatccg gtaactatca tcttgagtcc aacccggaaa gacacgacaa
     4741 aacgccactg gcagcagcca ttggtaactg agaattagtg gatttagata tcgagagtct
     4801 tgaagtggtg gcctaacaga ggctacactg aaaggacagt atttggtatc tgcgctccac
     4861 taaagccagt taccaggtta agcagttccc caactgactt aaccttcgat caaaccgcct
     4921 ccccaggcgg ttttttcgtt tacagagcag gagattacga cgatcgtaaa aggatctcaa
     4981 gaagatcctt tacggattcc cgacaccatc actctaga
//