LOCUS       pHK-507                 5095 bp ds-DNA     circular     27-FEB-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    37..71
                     /label="J23115"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(117..139)
                     /label="bs-2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(200..222)
                     /label="bs-3"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    283..297
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    298..403
                     /label="sgRNA-4"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    409..423
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    457..546
                     /label="ECK120029600 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    768..802
                     /label="P(BBa_J23100)"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(863..885)
                     /label="bs-4"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    957..971
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    993..998
                     /label="RBS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     gene            1006..1725
                     /label="sfGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1749..1809
                     /label="L3S2P21 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2065..2099
                     /label="J23100"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2121..2126
                     /label="RBS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2134..2841
                     /label="mCherry"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="label: mCherry"
     misc_feature    3019..3066
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: T7 terminator"
     gene            complement(3525..4340)
                     /label="KanR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     oriT            4450..5085
                     /label="ColA ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 GAATTCATAG GATAGATTCT GGAAACTTTA CCGTCCttta tagctagctc agcccttggt
       61 acaatgctag cAGATAGCCG TTACACAGGT GACACTTATT TCAGCCTGCG GTCCGGccaA
      121 GTACTTAGTA CACACTGATT CGCTGGGACG CCCGTAGAAC TCTGTGAGGA TAAAGTCTCC
      181 CTTACAGCCT GCGGTCCGGc caAGAGTACA CTAGCTCAGT CATCGCTGGG ACGCCCGTGA
      241 ACAGTTGCTC TGATTGAAAC CACGATTCAG CCTGCGGTCC GGCTGCCGTA TAGGCAGGAC
      301 ACATCTTAGA GTATGTAGTT TcAGAGCTAT GCTGGAAACA GCATAGCAAG TTgAAATAAG
      361 GCTAGTCCGT TATCAACTTG AAAAAGTGGC ACCGAGTCGG TGCGTTCACT GCCGTATAGG
      421 CAGTCGCTGG GACGCCCGAG GCTTACCTTA CCATGGTTCA GCCAAAAAAC TTAAGACCGC
      481 CGGTCTTGTC CACTACCTTG CAGTAATGCG GTGGACAGGA TCGGCGGTTT TCTTTTCTCT
      541 TCTCAATTCT TCTGACCTGT AACGAATAAT AGATAGTAAA GTAGTCTCCG ATTGAGTTTT
      601 CTCTGCCGAG TCCCACCCAG TTCTGTGATT TCAGTAAGTT GGTAATTGAT ACACTGTTGC
      661 GAGAACTGCT GCCTGGTAGT AGATAGGTTG TTATTGAGTA AGAAGGTAAA GTGAACGAAA
      721 TCCCTGAAAC TGAGACTGTA GAAAATAAGC TTCAGCCTGC GGTCCGGttg acggctagct
      781 cagtcctagg tacagtgcta gcTCGCTGGG ACGCCCGAGA TAGCCGTTAC ACAGGTGACA
      841 CTTATTTCAG CCTGCGGTCC GGccaTACAT ACTCTAAGAT GTGTCTCGCT GGGACGCCCG
      901 GGATCCAAGA GATTTCTACA CGATTGAGCA CTGTCTCAGC CTGCGGTCCG GGTTCACTGC
      961 CGTATAGGCA GTAATTTTGT TTAACTTTAA GAAGGAGATA TACATATGCG TAAAGGCGAA
     1021 GAACTGTTTA CCGGTGTGGT TCCGATTCTG GTGGAACTGG ACGGCGATGT TAATGGTCAT
     1081 AAATTCAGTG TTCGCGGCGA AGGTGAAGGC GATGCGACGA ACGGCAAACT GACCCTGAAA
     1141 TTTATCTGCA CCACGGGTAA ACTGCCGGTC CCGTGGCCGA CGCTGGTGAC CACGCTGACC
     1201 TATGGCGTTC AATGTTTTGC GCGTTACCCG GATCACATGA AACAGCACGA CTTTTTCAAA
     1261 TCGGCCATGC CGGAAGGCTA TGTGCAGGAA CGTACGATTA GCTTTAAAGA CGATGGTACG
     1321 TATAAAACCC GCGCGGAAGT GAAATTCGAA GGCGATACCC TGGTTAACCG TATCGAACTG
     1381 AAAGGTATCG ATTTCAAAGA AGACGGCAAT ATTCTGGGTC ATAAACTGGA ATATAACTTC
     1441 AATTCCCACA ACGTGTACAT CACCGCGGAT AAACAGAAAA ACGGCATTAA AGCCAATTTC
     1501 AAAATCCGCC ATAATGTGGA AGATGGTAGC GTTCAGCTGG CCGACCACTA TCAGCAAAAC
     1561 ACGCCGATTG GTGATGGCCC GGTCCTGCTG CCGGACAATC ACTACCTGAG TACCCAGTCC
     1621 GTGCTGTCAA AAGATCCGAA CGAAAAACGT GACCACATGG TCCTGCTGGA ATTTGTGACG
     1681 GCTGCGGGTA TCACCCACGG CATGGACGAA CTGTATAAAT AATAATCGCT GGGACGCCCG
     1741 CCTGCAGGCT CGGTACCAAA TTCCAGAAAA GAGGCCTCCC GAAAGGGGGG CCTTTTTTCG
     1801 TTTTGGTCCT AATAGATAAA GGATAGGTCT GGTAGTGTTG TTCGTTCTCG CAGGTAAATC
     1861 AATAATACTC AGCAGTTCCG TAGACTTTTC AGTGGGACAG GGTAGCGATA ACAGATAGAT
     1921 TGTAATAAGA CACAGTAGGT GCTCGTAGTT GCGTGAAGAG AACCGCTCAG GAAATCCAGT
     1981 CAGAAGTATT GGTAATCGTT GAAAACTCAg CTCCTAGGAT GCAGTGACTC GAGtTGTaAA
     2041 ACGACGGCCA gtcaattgga attcttgacg gctagctcag tcctaggtac agtgctagcT
     2101 AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGGTGA GCAAGGGCGA GGAGGATAAC
     2161 ATGGCCATCA TCAAGGAGTT CATGCGCTTC AAGGTGCACA TGGAGGGCTC CGTGAACGGC
     2221 CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC
     2281 AAGCTGAAGG TGACCAAGGG TGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG
     2341 TTCATGTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG
     2401 CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG
     2461 GTGACCGTGA CCCAGGACTC CTCtCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG
     2521 CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACtAT GGGCTGGGAG
     2581 GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCgCTGA AGGGCGAGAT CAAGCAGAGG
     2641 CTGAAGCTGA AGGACGGCGG CCACTACGAC GCTGAaGTCA AGACCACCTA CAAGGCCAAG
     2701 AAGCCCGTGC AGCTGCCCGG CGCgTACAAC GTCAACATCA AGTTGGACAT CACCTCCCAC
     2761 AACGAGGACT ACACCATCGT GGAACAGTAC GAACGCGCCG AGGGCCGCCA CTCCACCGGC
     2821 GGCATGGACG AGCTGTACAA GTAAactagt TgcggccgcC TGCGCACCAC CTGCAGGACC
     2881 TCAGCAATCG CTTGGACCAG CTTTCTTAAG GGTACCTATT ACAGCTGGAT CCGGCGCCGC
     2941 TCATTCGCTA ATCGCCACGa cgcgtAgtcg acCCGCCAAT TCCCATGGGG CGCGCCTGCT
     3001 GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
     3061 TTTTTGCCAG GCATCAAATA AAACGAAAGG CTCAGTCGGA AGACTGGGGC GAACGACGAG
     3121 TCACTGTTGA GGATAAATAC TTTCTCTACT AGGCGCCTGT TACACAGGTC CTCAGCGGCG
     3181 CGCCTTTGTC GGTGAACGCT CTCCTGAGTA GGACAAATCC GCCGGGAGCG GATTTGAACG
     3241 TTGTGAAGCA ACGGCCCGGA GGGTGGCGGG CAGGACGCCC GCCATAAACT GCCAGGCATC
     3301 AAACTAAGCA GAAGGCCATC CTGACGGATG GCCTTTTTGC GTTTCAGATC TACCGGTaaa
     3361 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga caagctgacg
     3421 accgggtctc cgcaagtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     3481 ttttctaaat acattcaaat atgtatccgc tcatgaatta attcttagaa aaactcatcg
     3541 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     3601 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     3661 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     3721 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     3781 ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     3841 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     3901 aatacgcggt cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     3961 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     4021 aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     4081 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     4141 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     4201 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
     4261 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga gcaagacgtt
     4321 tcccgttgaa tatggctcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4381 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggcatg
     4441 ctagcgcaga aacgtcctag aagatgccag gaggatactt agcagagaga caataaggcc
     4501 ggagcgaagc cgtttttcca taggctccgc ccccctgacg aacatcacga aatctgacgc
     4561 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgat
     4621 ggctccctct tgcgctctcc tgttcccgtc ctgcggcgtc cgtgttgtgg tggaggcttt
     4681 acccaaatca ccacgtcccg ttccgtgtag acagttcgct ccaagctggg ctgtgtgcaa
     4741 gaaccccccg ttcagcccga ctgctgcgcc ttatccggta actatcatct tgagtccaac
     4801 ccggaaagac acgacaaaac gccactggca gcagccattg gtaactgaga attagtggat
     4861 ttagatatcg agagtcttga agtggtggcc taacagaggc tacactgaaa ggacagtatt
     4921 tggtatctgc gctccactaa agccagttac caggttaagc agttccccaa ctgacttaac
     4981 cttcgatcaa accgcctccc caggcggttt tttcgtttac agagcaggag attacgacga
     5041 tcgtaaaagg atctcaagaa gatcctttac ggattcccga caccatcact ctaga
//