LOCUS       pHK-408                 5660 bp ds-DNA     circular     27-FEB-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    52..86
                     /label="J23108"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(147..169)
                     /label="bs-3"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    230..244
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    245..350
                     /label="sgRNA-5"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    356..370
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    386..553
                     /label="terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    599..633
                     /label="J23108"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(694..716)
                     /label="bs-2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    777..791
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    792..897
                     /label="sgRNA-4"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    903..917
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    951..1040
                     /label="ECK120029600 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1256..1290
                     /label="P(BBa_J23100)"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(1351..1373)
                     /label="bs-4"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(1434..1456)
                     /label="bs-5"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1522..1536
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1558..1563
                     /label="RBS"
                     /ApEinfo_revcolor="#fdc68a"
                     /ApEinfo_fwdcolor="#fdc68a"
     gene            1571..2290
                     /label="sfGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2314..2374
                     /label="L3S2P21 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2630..2664
                     /label="J23100"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2686..2691
                     /label="RBS"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             2699..3406
                     /label="mCherry"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="label: mCherry"
     misc_feature    3584..3631
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: T7 terminator"
     gene            complement(4090..4905)
                     /label="KanR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     oriT            5015..5650
                     /label="ColA ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 GAATTCATAG GATAGATTCT GGAAACTTTA CCGTCCCAGC CTGCGGTCCG Gctgacagct
       61 agctcagtcc taggtataat gctagcTCGC TGGGACGCCC GAGTGACGAC TGCGAAGTAA
      121 CCTCTATTTA TCAGCCTGCG GTCCGGccaA GAGTACACTA GCTCAGTCAT CGCTGGGACG
      181 CCCGGGGACT ACACTTACGA AACTATTGAT TGCTCAGCCT GCGGTCCGGC TGCCGTATAG
      241 GCAGAAGTGA GTCTGAGCTT AGATGTTTcA GAGCTATGCT GGAAACAGCA TAGCAAGTTg
      301 AAATAAGGCT AGTCCGTTAT CAACTTGAAA AAGTGGCACC GAGTCGGTGC GTTCACTGCC
      361 GTATAGGCAG TCGCTGGGAC GCCCGcagtt gATAAGTCCC TAACTttttg ttAtcAATAA
      421 AAAAGGCCtC CCGATTTGGG AGGCCTTAtt gttcgtccta gtaaaggaat attcagcaat
      481 ttgcccgtgc cgaagaaagg cccacccgtg aaggtgagcc agtgagttga ttgctacgta
      541 attagttagt tagAGAGCCG AATCGCACTT ATTTACAGTA GTTCAGCCTG CGGTCCGGct
      601 gacagctagc tcagtcctag gtataatgct agcTCGCTGG GACGCCCGTG AACAGTTGCT
      661 CTGATTGAAA CCACGATTCA GCCTGCGGTC CGGccaAGTA CTTAGTACAC ACTGATTCGC
      721 TGGGACGCCC GGTAGTGCTT ATCAGACCCA ATACTGTTGA ACAGCCTGCG GTCCGGCTGC
      781 CGTATAGGCA GGACACATCT TAGAGTATGT AGTTTcAGAG CTATGCTGGA AACAGCATAG
      841 CAAGTTgAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG TCGGTGCGTT
      901 CACTGCCGTA TAGGCAGTCG CTGGGACGCC CGAGGCTTAC CTTACCATGG TTCAGCCAAA
      961 AAACTTAAGA CCGCCGGTCT TGTCCACTAC CTTGCAGTAA TGCGGTGGAC AGGATCGGCG
     1021 GTTTTCTTTT CTCTTCTCAA TTCTTCTGAC CTGTAACGAA TAATAGATAG TAAAGTAGTC
     1081 TCCGATTGAG TTTTCTCTGC CGAGTCCCAC CCAGTTCTGT GATTTCAGTA AGTTGGTAAT
     1141 TGATACACTG TTGCGAGAAC TGCTGCCTGG TAGTAGATAG GTTGTTATTG AGTAAGAAGG
     1201 TAAAGTGAAC GAAATCCCTG AAACTGAGAC TGTAGAAAAT CAGCCTGCGG TCCGGttgac
     1261 ggctagctca gtcctaggta cagtgctagc TCGCTGGGAC GCCCGAGATA GCCGTTACAC
     1321 AGGTGACACT TATTTCAGCC TGCGGTCCGG ccaTACATAC TCTAAGATGT GTCTCGCTGG
     1381 GACGCCCGTA GAACTCTGTG AGGATAAAGT CTCCCTTACA GCCTGCGGTC CGGccaATCT
     1441 AAGCTCAGAC TCACTTTCGC TGGGACGCCC GAAGAGATTT CTACACGATT GAGCACTGTC
     1501 TCAGCCTGCG GTCCGGGTTC ACTGCCGTAT AGGCAGTAAT TTTGTTTAAC TTTAAGAAGG
     1561 AGATATACAT ATGCGTAAAG GCGAAGAACT GTTTACCGGT GTGGTTCCGA TTCTGGTGGA
     1621 ACTGGACGGC GATGTTAATG GTCATAAATT CAGTGTTCGC GGCGAAGGTG AAGGCGATGC
     1681 GACGAACGGC AAACTGACCC TGAAATTTAT CTGCACCACG GGTAAACTGC CGGTCCCGTG
     1741 GCCGACGCTG GTGACCACGC TGACCTATGG CGTTCAATGT TTTGCGCGTT ACCCGGATCA
     1801 CATGAAACAG CACGACTTTT TCAAATCGGC CATGCCGGAA GGCTATGTGC AGGAACGTAC
     1861 GATTAGCTTT AAAGACGATG GTACGTATAA AACCCGCGCG GAAGTGAAAT TCGAAGGCGA
     1921 TACCCTGGTT AACCGTATCG AACTGAAAGG TATCGATTTC AAAGAAGACG GCAATATTCT
     1981 GGGTCATAAA CTGGAATATA ACTTCAATTC CCACAACGTG TACATCACCG CGGATAAACA
     2041 GAAAAACGGC ATTAAAGCCA ATTTCAAAAT CCGCCATAAT GTGGAAGATG GTAGCGTTCA
     2101 GCTGGCCGAC CACTATCAGC AAAACACGCC GATTGGTGAT GGCCCGGTCC TGCTGCCGGA
     2161 CAATCACTAC CTGAGTACCC AGTCCGTGCT GTCAAAAGAT CCGAACGAAA AACGTGACCA
     2221 CATGGTCCTG CTGGAATTTG TGACGGCTGC GGGTATCACC CACGGCATGG ACGAACTGTA
     2281 TAAATAATAA TCGCTGGGAC GCCCGCCTGC AGGCTCGGTA CCAAATTCCA GAAAAGAGGC
     2341 CTCCCGAAAG GGGGGCCTTT TTTCGTTTTG GTCCTAATAG ATAAAGGATA GGTCTGGTAG
     2401 TGTTGTTCGT TCTCGCAGGT AAATCAATAA TACTCAGCAG TTCCGTAGAC TTTTCAGTGG
     2461 GACAGGGTAG CGATAACAGA TAGATTGTAA TAAGACACAG TAGGTGCTCG TAGTTGCGTG
     2521 AAGAGAACCG CTCAGGAAAT CCAGTCAGAA GTATTGGTAA TCGTTGAAAA CTCAgCTCCT
     2581 AGGATGCAGT GACTCGAGtT GTaAAACGAC GGCCAgtcaa ttggaattct tgacggctag
     2641 ctcagtccta ggtacagtgc tagcTAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT
     2701 GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT
     2761 GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG
     2821 CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT
     2881 CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC
     2941 CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT
     3001 GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCtC TGCAGGACGG
     3061 CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT
     3121 GCAGAAGAAG ACtATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC
     3181 gCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA
     3241 aGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCgT ACAACGTCAA
     3301 CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG
     3361 CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGTAAa ctagtTgcgg
     3421 ccgcCTGCGC ACCACCTGCA GGACCTCAGC AATCGCTTGG ACCAGCTTTC TTAAGGGTAC
     3481 CTATTACAGC TGGATCCGGC GCCGCTCATT CGCTAATCGC CACGacgcgt AgtcgacCCG
     3541 CCAATTCCCA TGGGGCGCGC CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG
     3601 GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCCAGGCATC AAATAAAACG AAAGGCTCAG
     3661 TCGGAAGACT GGGGCGAACG ACGAGTCACT GTTGAGGATA AATACTTTCT CTACTAGGCG
     3721 CCTGTTACAC AGGTCCTCAG CGGCGCGCCT TTGTCGGTGA ACGCTCTCCT GAGTAGGACA
     3781 AATCCGCCGG GAGCGGATTT GAACGTTGTG AAGCAACGGC CCGGAGGGTG GCGGGCAGGA
     3841 CGCCCGCCAT AAACTGCCAG GCATCAAACT AAGCAGAAGG CCATCCTGAC GGATGGCCTT
     3901 TTTGCGTTTC AGATCTACCG GTaaaccagc aatagacata agcggctatt taacgaccct
     3961 gccctgaacc gacgacaagc tgacgaccgg gtctccgcaa gtggcacttt tcggggaaat
     4021 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4081 aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
     4141 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
     4201 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
     4261 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
     4321 agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc
     4381 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
     4441 tcgtgattgc gcctgagcga gacgaaatac gcggtcgctg ttaaaaggac aattacaaac
     4501 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
     4561 atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa
     4621 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
     4681 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
     4741 tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga
     4801 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
     4861 taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcatactct tcctttttca
     4921 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     4981 ttagaaaaat aaacaaatag gcatgctagc gcagaaacgt cctagaagat gccaggagga
     5041 tacttagcag agagacaata aggccggagc gaagccgttt ttccataggc tccgcccccc
     5101 tgacgaacat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata
     5161 aagataccag gcgtttcccc ctgatggctc cctcttgcgc tctcctgttc ccgtcctgcg
     5221 gcgtccgtgt tgtggtggag gctttaccca aatcaccacg tcccgttccg tgtagacagt
     5281 tcgctccaag ctgggctgtg tgcaagaacc ccccgttcag cccgactgct gcgccttatc
     5341 cggtaactat catcttgagt ccaacccgga aagacacgac aaaacgccac tggcagcagc
     5401 cattggtaac tgagaattag tggatttaga tatcgagagt cttgaagtgg tggcctaaca
     5461 gaggctacac tgaaaggaca gtatttggta tctgcgctcc actaaagcca gttaccaggt
     5521 taagcagttc cccaactgac ttaaccttcg atcaaaccgc ctccccaggc ggttttttcg
     5581 tttacagagc aggagattac gacgatcgta aaaggatctc aagaagatcc tttacggatt
     5641 cccgacacca tcactctaga
//