LOCUS       pHK-009                 4776 bp ds-DNA     circular     27-FEB-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    76..123
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: T7 terminator"
     gene            complement(582..1397)
                     /label="KanR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     oriT            1507..2142
                     /label="ColA ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2213..2302
                     /label="ECK120029600 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2524..2558
                     /label="P(BBa_J23100)"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(2619..2641)
                     /label="bs-2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(2702..2724)
                     /label="bs-3"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2790..2804
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2826..2831
                     /label="RBS"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     gene            2839..3558
                     /label="sfGFP "
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3582..3642
                     /label="L3S2P21 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3898..3932
                     /label="J23100"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3954..3959
                     /label="RBS"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             3967..4674
                     /label="mCherry"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="label: mCherry"
ORIGIN
        1 TTCGCTAATC GCCACGacgc gtAgtcgacC CGCCAATTCC CATGGGGCGC GCCTGCTGCC
       61 ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
      121 TTGCCAGGCA TCAAATAAAA CGAAAGGCTC AGTCGGAAGA CTGGGGCGAA CGACGAGTCA
      181 CTGTTGAGGA TAAATACTTT CTCTACTAGG CGCCTGTTAC ACAGGTCCTC AGCGGCGCGC
      241 CTTTGTCGGT GAACGCTCTC CTGAGTAGGA CAAATCCGCC GGGAGCGGAT TTGAACGTTG
      301 TGAAGCAACG GCCCGGAGGG TGGCGGGCAG GACGCCCGCC ATAAACTGCC AGGCATCAAA
      361 CTAAGCAGAA GGCCATCCTG ACGGATGGCC TTTTTGCGTT TCAGATCTAC CGGTaaacca
      421 gcaatagaca taagcggcta tttaacgacc ctgccctgaa ccgacgacaa gctgacgacc
      481 gggtctccgc aagtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      541 tctaaataca ttcaaatatg tatccgctca tgaattaatt cttagaaaaa ctcatcgagc
      601 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
      661 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
      721 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
      781 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
      841 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
      901 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
      961 acgcggtcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     1021 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     1081 gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     1141 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     1201 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     1261 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     1321 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc
     1381 cgttgaatat ggctcatact cttccttttt caatattatt gaagcattta tcagggttat
     1441 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggcatgcta
     1501 gcgcagaaac gtcctagaag atgccaggag gatacttagc agagagacaa taaggccgga
     1561 gcgaagccgt ttttccatag gctccgcccc cctgacgaac atcacgaaat ctgacgctca
     1621 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctgatggc
     1681 tccctcttgc gctctcctgt tcccgtcctg cggcgtccgt gttgtggtgg aggctttacc
     1741 caaatcacca cgtcccgttc cgtgtagaca gttcgctcca agctgggctg tgtgcaagaa
     1801 ccccccgttc agcccgactg ctgcgcctta tccggtaact atcatcttga gtccaacccg
     1861 gaaagacacg acaaaacgcc actggcagca gccattggta actgagaatt agtggattta
     1921 gatatcgaga gtcttgaagt ggtggcctaa cagaggctac actgaaagga cagtatttgg
     1981 tatctgcgct ccactaaagc cagttaccag gttaagcagt tccccaactg acttaacctt
     2041 cgatcaaacc gcctccccag gcggtttttt cgtttacaga gcaggagatt acgacgatcg
     2101 taaaaggatc tcaagaagat cctttacgga ttcccgacac catcactcta gaGAATTCAT
     2161 AGGATAGATT CTGGAAACTT TACCGTCCGA GCTCAGGCTT ACCTTACCAT GGTTCAGCCA
     2221 AAAAACTTAA GACCGCCGGT CTTGTCCACT ACCTTGCAGT AATGCGGTGG ACAGGATCGG
     2281 CGGTTTTCTT TTCTCTTCTC AATTCTTCTG ACCTGTAACG AATAATAGAT AGTAAAGTAG
     2341 TCTCCGATTG AGTTTTCTCT GCCGAGTCCC ACCCAGTTCT GTGATTTCAG TAAGTTGGTA
     2401 ATTGATACAC TGTTGCGAGA ACTGCTGCCT GGTAGTAGAT AGGTTGTTAT TGAGTAAGAA
     2461 GGTAAAGTGA ACGAAATCCC TGAAACTGAG ACTGTAGAAA ATAAGCTTCA GCCTGCGGTC
     2521 CGGttgacgg ctagctcagt cctaggtaca gtgctagcTC GCTGGGACGC CCGAGATAGC
     2581 CGTTACACAG GTGACACTTA TTTCAGCCTG CGGTCCGGcc aAGTACTTAG TACACACTGA
     2641 TTCGCTGGGA CGCCCGTAGA ACTCTGTGAG GATAAAGTCT CCCTTACAGC CTGCGGTCCG
     2701 GccaAGAGTA CACTAGCTCA GTCATCGCTG GGACGCCCGA AGAGATTTCT ACACGATTGA
     2761 GCACTGTCTC AGCCTGCGGT CCGGGTTCAC TGCCGTATAG GCAGTAATTT TGTTTAACTT
     2821 TAAGAAGGAG ATATACATAT GCGTAAAGGC GAAGAACTGT TTACCGGTGT GGTTCCGATT
     2881 CTGGTGGAAC TGGACGGCGA TGTTAATGGT CATAAATTCA GTGTTCGCGG CGAAGGTGAA
     2941 GGCGATGCGA CGAACGGCAA ACTGACCCTG AAATTTATCT GCACCACGGG TAAACTGCCG
     3001 GTCCCGTGGC CGACGCTGGT GACCACGCTG ACCTATGGCG TTCAATGTTT TGCGCGTTAC
     3061 CCGGATCACA TGAAACAGCA CGACTTTTTC AAATCGGCCA TGCCGGAAGG CTATGTGCAG
     3121 GAACGTACGA TTAGCTTTAA AGACGATGGT ACGTATAAAA CCCGCGCGGA AGTGAAATTC
     3181 GAAGGCGATA CCCTGGTTAA CCGTATCGAA CTGAAAGGTA TCGATTTCAA AGAAGACGGC
     3241 AATATTCTGG GTCATAAACT GGAATATAAC TTCAATTCCC ACAACGTGTA CATCACCGCG
     3301 GATAAACAGA AAAACGGCAT TAAAGCCAAT TTCAAAATCC GCCATAATGT GGAAGATGGT
     3361 AGCGTTCAGC TGGCCGACCA CTATCAGCAA AACACGCCGA TTGGTGATGG CCCGGTCCTG
     3421 CTGCCGGACA ATCACTACCT GAGTACCCAG TCCGTGCTGT CAAAAGATCC GAACGAAAAA
     3481 CGTGACCACA TGGTCCTGCT GGAATTTGTG ACGGCTGCGG GTATCACCCA CGGCATGGAC
     3541 GAACTGTATA AATAATAATC GCTGGGACGC CCGCCTGCAG GCTCGGTACC AAATTCCAGA
     3601 AAAGAGGCCT CCCGAAAGGG GGGCCTTTTT TCGTTTTGGT CCTAATAGAT AAAGGATAGG
     3661 TCTGGTAGTG TTGTTCGTTC TCGCAGGTAA ATCAATAATA CTCAGCAGTT CCGTAGACTT
     3721 TTCAGTGGGA CAGGGTAGCG ATAACAGATA GATTGTAATA AGACACAGTA GGTGCTCGTA
     3781 GTTGCGTGAA GAGAACCGCT CAGGAAATCC AGTCAGAAGT ATTGGTAATC GTTGAAAACT
     3841 CAgCTCCTAG GATGCAGTGA CTCGAGtTGT aAAACGACGG CCAgtcaatt ggaattcttg
     3901 acggctagct cagtcctagg tacagtgcta gcTAATTTTG TTTAACTTTA AGAAGGAGAT
     3961 ATACATATGG TGAGCAAGGG CGAGGAGGAT AACATGGCCA TCATCAAGGA GTTCATGCGC
     4021 TTCAAGGTGC ACATGGAGGG CTCCGTGAAC GGCCACGAGT TCGAGATCGA GGGCGAGGGC
     4081 GAGGGCCGCC CCTACGAGGG CACCCAGACC GCCAAGCTGA AGGTGACCAA GGGTGGCCCC
     4141 CTGCCCTTCG CCTGGGACAT CCTGTCCCCT CAGTTCATGT ACGGCTCCAA GGCCTACGTG
     4201 AAGCACCCCG CCGACATCCC CGACTACTTG AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG
     4261 GAGCGCGTGA TGAACTTCGA GGACGGCGGC GTGGTGACCG TGACCCAGGA CTCCTCtCTG
     4321 CAGGACGGCG AGTTCATCTA CAAGGTGAAG CTGCGCGGCA CCAACTTCCC CTCCGACGGC
     4381 CCCGTAATGC AGAAGAAGAC tATGGGCTGG GAGGCCTCCT CCGAGCGGAT GTACCCCGAG
     4441 GACGGCGCgC TGAAGGGCGA GATCAAGCAG AGGCTGAAGC TGAAGGACGG CGGCCACTAC
     4501 GACGCTGAaG TCAAGACCAC CTACAAGGCC AAGAAGCCCG TGCAGCTGCC CGGCGCgTAC
     4561 AACGTCAACA TCAAGTTGGA CATCACCTCC CACAACGAGG ACTACACCAT CGTGGAACAG
     4621 TACGAACGCG CCGAGGGCCG CCACTCCACC GGCGGCATGG ACGAGCTGTA CAAGTAAact
     4681 agtTgcggcc gcCTGCGCAC CACCTGCAGG ACCTCAGCAA TCGCTTGGAC CAGCTTTCTT
     4741 AAGGGTACCT ATTACAGCTG GATCCGGCGC CGCTCA
//