LOCUS       pHK-052.3               2607 bp ds-DNA     circular     27-FEB-2025
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: pET-17b.txt pET-17b
FEATURES             Location/Qualifiers
     rep_origin      complement(44..793)
                     /label="RSF ori"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="Plasmids containing the RSF 1030 origin of replication can be propagated in E. coli cells that containadditional plasmids with compatible origins."
                     /direction="LEFT"
     misc_feature    817..921
                     /label="T1 transcriptional terminator"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     gene            complement(982..1515)
                     /label="gentamicin resistance"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     terminator      complement(1739..1867)
                     /label="T7_terminator"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(1931..1945)
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(1951..2056)
                     /label="sgRNA-3"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(2057..2071)
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(2092..2126)
                     /label="J23119"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      2301..2607
                     /label="F1 ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 atttcgcggg atcgagatct cgggccggct cgccatcgtc cagcttccgc ttcctcgctc
       61 actgactcgc tacgctcggt cgttcgactg cggcgagcgg tgtcagctca ctcaaaagcg
      121 gtaatacggt tatccacaga atcaggggat aaagccggaa agaacatgtg agcaaaaagc
      181 aaagcaccgg aagaagccaa cgccgcaggc gtttttccat aggctccgcc cccctgacga
      241 gcatcacaaa aatcgacgct caagccagag gtggcgaaac ccgacaggac tataaagata
      301 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      361 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      421 ttggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      481 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      541 acacgactta tcgccactgg cagcagccat tggtaactga tttagaggac tttgtcttga
      601 agttatgcac ctgttaaggc taaactgaaa gaacagattt tggtgagtgc ggtcctccaa
      661 cccacttacc ttggttcaaa gagttggtag ctcagcgaac cttgagaaaa ccaccgttgg
      721 tagcggtggt ttttctttat ttatgagatg atgaatcaat cggtctatca agtcaacgaa
      781 cagctattcc gttgcggcca tcgtccacat atccactcta gggcggcgga tttgtcctac
      841 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
      901 cctttcgttt tatttgatgc cgacgtccaa tttaccgaac aactccgcgg ccgggaagcc
      961 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     1021 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     1081 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     1141 cgcggtggca atgccctgcc tccggtgctc tccggagact gcgagatcat agatatagat
     1201 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     1261 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     1321 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     1381 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     1441 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     1501 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     1561 cttgctgctt ggatgcccga ggcataggct gtacaaaaaa acagtcataa caagccatga
     1621 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     1681 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aagacgtcat
     1741 ccggatatag ttcctccttt cagcaaaaaa cccctcaaga cccgtttaga ggccccaagg
     1801 ggttatgcta gttattgctc agcggtggca gcagccaact cagcttcctt tcgggctttg
     1861 ttagcagccg gatctgctcg agcggccgcc agtgtgatgg atatctgcag aatCGGGCGT
     1921 CCCAGCGAGC CTGCCTATAC GGCAGTGAAC GCACCGACTC GGTGCCACTT TTTCAAGTTG
     1981 ATAACGGACT AGCCTTATTT cAACTTGCTA TGCTGTTTCC AGCATAGCTC TgAAACAGAG
     2041 TACACTAGCT CAGTCACTGC CTATACGGCA GTGAACCCGG ACCGCAGGCT GGCTAGCATT
     2101 ATACCTAGGA CTGAGCTAGC TGTCAAACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA
     2161 AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT
     2221 AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA
     2281 TGGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA
     2341 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG
     2401 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT
     2461 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG
     2521 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA
     2581 GTGGACTCTT GTTCCAAACT GGAACAA
//