LOCUS       pHK-352.3               2607 bp ds-DNA     circular     27-FEB-2025
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: pET-17b.txt pET-17b
FEATURES             Location/Qualifiers
     rep_origin      complement(1..750)
                     /label="RSF ori"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="Plasmids containing the RSF 1030 origin of replication can be propagated in E. coli cells that containadditional plasmids with compatible origins."
                     /direction="LEFT"
     misc_feature    774..878
                     /label="T1 transcriptional terminator"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     gene            complement(939..1472)
                     /label="gentamicin resistance"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     terminator      complement(1696..1824)
                     /label="T7_terminator"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(1888..1902)
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(1908..2013)
                     /label="sgRNA-3"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(2014..2028)
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     source          complement(2049..2083)
                     /label="J23110"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="organism: pET-17b mol_type: other DNA"
     rep_origin      2258..2564
                     /label="F1 ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggtgt
       61 cagctcactc aaaagcggta atacggttat ccacagaatc aggggataaa gccggaaaga
      121 acatgtgagc aaaaagcaaa gcaccggaag aagccaacgc cgcaggcgtt tttccatagg
      181 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gccagaggtg gcgaaacccg
      241 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      301 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      361 tctcatagct cacgctgttg gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      421 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      481 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccattgg taactgattt
      541 agaggacttt gtcttgaagt tatgcacctg ttaaggctaa actgaaagaa cagattttgg
      601 tgagtgcggt cctccaaccc acttaccttg gttcaaagag ttggtagctc agcgaacctt
      661 gagaaaacca ccgttggtag cggtggtttt tctttattta tgagatgatg aatcaatcgg
      721 tctatcaagt caacgaacag ctattccgtt gcggccatcg tccacatatc cactctaggg
      781 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
      841 ccagtctttc gactgagcct ttcgttttat ttgatgccga cgtccaattt accgaacaac
      901 tccgcggccg ggaagccgat ctcggcttga acgaattgtt aggtggcggt acttgggtcg
      961 atatcaaagt gcatcacttc ttcccgtatg cccaactttg tatagagagc cactgcggga
     1021 tcgtcaccgt aatctgcttg cacgtagatc acataagcac caagcgcgtt ggcctcatgc
     1081 ttgaggagat tgatgagcgc ggtggcaatg ccctgcctcc ggtgctctcc ggagactgcg
     1141 agatcataga tatagatctc actacgcggc tgctcaaact tgggcagaac gtaagccgcg
     1201 agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg
     1261 agcaagttcc cgaggtaatc ggagtccggc tgatgttggg agtaggtggc tacgtctccg
     1321 aactcacgac cgaaaagatc aagagcagcc cgcatggatt tgacttggtc agggccgagc
     1381 ctacatgtgc gaatgatgcc catacttgag ccacctaact ttgttttagg gcgactgccc
     1441 tgctgcgtaa catcgttgct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     1501 acccacggcg taacgcgctt gctgcttgga tgcccgaggc ataggctgta caaaaaaaca
     1561 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
     1621 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agtttacgaa ccgaacaggc
     1681 ttatgtcaag acgtcatccg gatatagttc ctcctttcag caaaaaaccc ctcaagaccc
     1741 gtttagaggc cccaaggggt tatgctagtt attgctcagc ggtggcagca gccaactcag
     1801 cttcctttcg ggctttgtta gcagccggat ctgctcgagc ggccgccagt gtgatggata
     1861 tctgcagaat CGGGCGTCCC AGCGAGCCTG CCTATACGGC AGTGAACGCA CCGACTCGGT
     1921 GCCACTTTTT CAAGTTGATA ACGGACTAGC CTTATTTcAA CTTGCTATGC TGTTTCCAGC
     1981 ATAGCTCTgA AACAGAGTAC ACTAGCTCAG TCACTGCCTA TACGGCAGTG AACCCGGACC
     2041 GCAGGCTGgc tagcattgta cctaggactg agctagccgt aaaACTGGCC GTCGTTTTAC
     2101 AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC
     2161 CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC
     2221 GCAGCCTGAA TGGCGAATGG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT
     2281 GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT
     2341 CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT
     2401 CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG
     2461 TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA
     2521 GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAAatttcg cgggatcgag
     2581 atctcgggcc ggctcgccat cgtccag
//