LOCUS       pHK-318                 4763 bp ds-DNA     circular     27-FEB-2025
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: 2 pCDF-MCS.gbk.txt
FEATURES             Location/Qualifiers
     gene            complement(82..870)
                     /label="SpecR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     oriT            complement(1010..1748)
                     /label="CDF ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(1932..2573)
                     /label="TetR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     terminator      2722..2816
                     /label="double terminator B0014"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(2853..3608)
                     /label="luxR"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(3625..3775)
                     /label="luxL promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3821..3892
                     /label="P(Lux)R (BBa_R0062) - LuxR&AHL-regulated prom."
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3874..3892
                     /label="tetO"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3929..3934
                     /label="RBS"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             3941..4162
                     /label="M13 gene VIII"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4163..4223
                     /label="L3S2P21 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
ORIGIN
        1 acgaccctgc cctgaaccga cgaccgggtc atcgtggccg gatcttgcgg cccctcggct
       61 tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
      121 gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
      181 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
      241 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
      301 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
      361 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
      421 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
      481 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
      541 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
      601 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
      661 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
      721 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
      781 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
      841 cgatacttcg gcgatcaccg cttccctcat actcttcctt tttcaatatt attgaagcat
      901 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
      961 aatagctagc tcactcggtc gctacgctcc gggcgtgaga ctgcggcggg cgctgcggac
     1021 acatacaaag ttacccacag attccgtgga taagcagggg actaacatgt gaggcaaaac
     1081 agcagggccg cgccggtggc gtttttccat aggctccgcc ctcctgccag agttcacata
     1141 aacagacgct tttccggtgc atctgtggga gccgtgaggc tcaaccatga atctgacagt
     1201 acgggcgaaa cccgacagga cttaaagatc cccaccgttt ccggcgggtc gctccctctt
     1261 gcgctctcct gttccgaccc tgccgtttac cggatacctg ttccgccttt ctcccttacg
     1321 ggaagtgtgg cgctttctca tagctcacac actggtatct cggctcggtg taggtcgttc
     1381 gctccaagct gggctgtaag caagaactcc ccgttcagcc cgactgctgc gccttatccg
     1441 gtaactgttc acttgagtcc aacccggaaa agcacggtaa aacgccactg gcagcagcca
     1501 ttggtaactg ggagttcgca gaggatttgt ttagctaaac acgcggttgc tcttgaagtg
     1561 tgcgccaaag tccggctaca ctggaaggac agatttggtt gctgtgctct gcgaaagcca
     1621 gttaccacgg ttaagcagtt ccccaactga cttaaccttc gatcaaacca cctccccagg
     1681 tggttttttc gtttacaggg caaaagatta cgcgcagaaa aaaaggatct caagaagatc
     1741 ctttgatctt ttctactgaa ccgctctaga tttcagtgca atttatctct tcaaatgtag
     1801 cacctgaagt cagccccata cgatataagt tgtaattctc atgttagtca tgccccgcgc
     1861 ccaccggaag gagctgactg ggttgCTCCT AGGATGCAGT GACTCGcaca tttccccgaa
     1921 aagtgccacc tttaggaccc actttcacat ttaagttgtt tttctaatcc gcatataatc
     1981 aattcaaggc cgaataagaa ggctggctct gcaccttggt gatcaaataa ttcgatagct
     2041 tgtcgtaata atggcggcat actatcagta gtaggtgttt ccctttcttc tttagcgact
     2101 tgatgctctt gatcttccaa tacgcaacct aaagtaaaat gccccacagc gctgagtgca
     2161 tataatgcat tctctagtga aaaaccttgt tggcataaaa aggctaattg attttcgaga
     2221 gtttcatact gtttttctgt aggccgtgta cctaaatgta cttttgctcc atcgcgatga
     2281 cttagtaaag cacatctaaa acttttagcg ttattacgta aaaaatcttg ccagctttcc
     2341 ccttctaaag ggcaaaagtg agtatggtgc ctatctaaca tctcaatggc taaggcgtcg
     2401 agcaaagccc gcttattttt tacatgccaa tacaatgtag gctgctctac acctagcttc
     2461 tgggcgagtt tacgggttgt taaaccttcg attccgacct cattaagcag ctctaatgcg
     2521 ctgttaatca ctttactttt atctaatctg gaCATTTTTT TCCTCCTGGA TCCttaattt
     2581 ttaaagtatg ggcaatcaat tgctcctgtt aaaattgctt tagaaatact ttggcagcgg
     2641 tttgttgtat tgagtttcat ttgcgcattg gttaaatgga aagtgacagt acgctcactg
     2701 cGCCGTCACT GCGTCTTTTA Ctcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     2761 tatactagag agagaatata aaaagccaga ttattaatcc ggctttttta ttatttGAAT
     2821 TCGAAATCCC TGAAACTGAG ACTGTAGAAA ATTTATTAAT TTTTGAAGTA CGGACAGTCG
     2881 ATAGCGCCGG TCAGGATTGC TTTGGAGATG GATTGGCAGC GATTCGTGGT GTTCAGTTTC
     2941 ATTTGCGCGT TCGTCAGATG AAACGTAACG GTACGTTCGG AACAACCCAG GATTTTACTA
     3001 ATATCCCAAG AGCTTTTGCC TTCACAGGCC CACGCCAGGC ATTCTTTTTC GCGTTTGGTC
     3061 AGGTCATTGT TACTTTTGTT GTTAGCGATG TTGATTTTAC GGTAGTTATC AACCAGAGAC
     3121 GGCACGATCA GCGGAATATT CATGCATGCA TGCAGGAACA GGCTGTCAAT ATAGTTATCT
     3181 TTTTCAGAGT GAGCGAAGCT CAGCATACCA AAGCCATTGT TTGCGGTATG GATCGGGAAC
     3241 GAAAAACCCG TAATCAGGCC GCTGGTTTTG GCTTCTTTGA TCACGTTCGG AGATTTTTTG
     3301 TTGACCGCAT TGTTTTCAAA GATATTCCAG TTAATCGGGG AGTGATTGGA GTTACTGTAG
     3361 TCCACGATCG GATCATATTT AATCAGATTG GCGTCATCGT AATACTGACG CCATTTTTTC
     3421 GGGTAGTTGT CCAGGATCGA AATATCTGAT TTGACCATAC TGTGCGGATA GATAATCGCC
     3481 AGCAGGTAAT ATTCGCAATG AACCATTTTG GTCATATCGC TCAGGCACTG GTTGATGTCG
     3541 TTGTTTGAGC GACAAGCTTT GATTTTGTTG ATGATGCGGT ACGTATCGTC GGCGTTGATG
     3601 TTTTTCATAC CCATCTCTTT ATCCTTACCT ATTGTTTGTC GCAAGTTTTG CGTGTTATAT
     3661 ATCATTAAAA CGGTAATGGA TTGACATTTG ATTCTAATAA ATTGGATTTT TGTCACACTA
     3721 TTGTATCGCT GGGAATACAA TTACTTAACA TAAGCACCTG TAGGATCGTA CAGGTCAATA
     3781 AACAGTTGAT AGGGCTTCTC CGTTACAGCC TGCGGTCCGG acctgtagga tcgtacaggt
     3841 ttacgcaaga aaatggtttg ttatagtcga atatccctat cagtgataga gaTCGCTGGG
     3901 ACGCCCGTAA TTTTGTTTAA CTTTAAGAAG GAGATATACC atgaaaaagt ctttagccct
     3961 caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
     4021 cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta
     4081 tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
     4141 attcacctcg aaagcaagct gaCTCGGTAC CAAATTCCAG AAAAGAGGCC TCCCGAAAGG
     4201 GGGGCCTTTT TTCGTTTTGG TCCTAATAGA TAAAGGATAG GTCTGGTAGT GTTGTTCGTT
     4261 CTCGCAGGTA AATCAATAAT ACTCAGCAGT TCCGTAGACT TTTCAGTGGG ACAGGGTAGC
     4321 GATAACAGAT AGATTGTAAT AAGACACAGT AGGTGCTCGT AGTTGCGTGA AGAGAACCGC
     4381 TCAGGAAATC CAGTCAGAAG TATTGGTAAT CGTTGAAAAC TCAGTCGACT GCTGCCACCG
     4441 CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC
     4501 CAGGCATCAA ATAAAACGAA AGGCTCAGTC GGAAGACTGG GCCTTTCGTT TTATCTGTTG
     4561 TTTGTCGGTG AACGCTCTCC TGAGTAGGAC AAATCCGCCG GGAGCGGATT TGAACGTTGT
     4621 GAAGCAACGG CCCGGAGGGT GGCGGGCAGG ACGCCCGCCA TAAACTGCCA GGCATCAAAC
     4681 TAAGCAGAAG GCCATCCTGA CGGATGGCCT TTTTGCGTTT CAGATCTacc ggtaaaccag
     4741 caatagacat aagcggctat tta
//