LOCUS       pHK-101                 5410 bp ds-DNA     circular     27-FEB-2025
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: 2 pCDF-MCS.gbk.txt
FEATURES             Location/Qualifiers
     CDS             complement(1..1083)
                     /label="lacI"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     promoter        1203..1257
                     /label="Ptac"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1303..1317
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1322..1327
                     /label="RBS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1330..2076
                     /label="lambda repressor cI"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2082..2096
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2118..2207
                     /label="ECK120029600 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2429..2463
                     /label="J23119"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    2524..2572
                     /label="cI binding site"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2609..2614
                     /label="RBS"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             2621..2842
                     /label="M13 gene VIII"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2843..2903
                     /label="L3S2P21 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     gene            complement(3525..4313)
                     /label="SpecR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     oriT            complement(4453..5191)
                     /label="CDF ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA
       61 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCCAGGGTG GTTTTTCTTT TCACCAGTGA
      121 GACTGGCAAC AGCTGATTGC CCTTCACCGC CTGGCCCTGA GAGAGTTGCA GCAAGCGGTC
      181 CACGCTGGTT TGCCCCAGCA GGCGAAAATC CTGTTTGATG GTGGTTAACG GCGGGATATA
      241 ACATGAGCTA TCTTCGGTAT CGTCGTATCC CACTACCGAG ATATCCGCAC CAACGCGCAG
      301 CCCGGACTCG GTAATGGCGC GCATTGCGCC CAGCGCCATC TGATCGTTGG CAACCAGCAT
      361 CGCAGTGGGA ACGATGCCCT CATTCAGCAT TTGCATGGTT TGTTGAAAAC CGGACATGGC
      421 ACTCCAGTCG CCTTCCCGTT CCGCTATCGG CTGAATTTGA TTGCGAGTGA GATATTTATG
      481 CCAGCCAGCC AGACGCAGAC GCGCCGAGAC AGAACTTAAT GGGCCCGCTA ACAGCGCGAT
      541 TTGCTGGTGA CCCAATGCGA CCAGATGCTC CACGCCCAGT CGCGTACCGT CCTCATGGGA
      601 GAAAATAATA CTGTTGATGG GTGTCTGGTC AGAGACATCA AGAAATAACG CCGGAACATT
      661 AGTGCAGGCA GCTTCCACAG CAATGGCATC CTGGTCATCC AGCGGATAGT TAATGATCAG
      721 CCCACTGACG CGTTGCGCGA GAAGATTGTG CACCGCCGCT TTACAGGCTT CGACGCCGCT
      781 TCGTTCTACC ATCGACACCA CCACGCTGGC ACCCAGTTGA TCGGCGCGAG ATTTAATCGC
      841 CGCGACAATT TGCGACGGCG CGTGCAGGGC CAGACTGGAG GTGGCAACGC CAATCAGCAA
      901 CGACTGTTTG CCCGCCAGTT GTTGTGCCAC GCGGTTGGGA ATGTAATTCA GCTCCGCCAT
      961 CGCCGCTTCC ACTTTTTCCC GCGTTTTCGC AGAAACGTGG CTGGCCTGGT TCACCACGCG
     1021 GGAAACGGTC TGATAAGAGA CACCGGCATA CTCTGCGACA TCGTATAACG TTACTGGTTT
     1081 CATATTCACC ACCCTGAATT GACTCTCTTC CGGGCGCTAT CATGCCATAC CGCGAAAGGT
     1141 TTTGCGCCAT TCGATGGCGC GCCGCcattg cggccgcGCC GTCACTGCGT CTTTTACgag
     1201 ctgttgacaa ttaatcatcg gctcgtataa tgtgGGAATT GTGAGCGGAT AACAATTAAG
     1261 AGATTTCTAC ACGATTGAGC ACTGTCTCAG CCTGCGGTCC GGCTGCCGTA TAGGCAGaaa
     1321 gaggagaaaa tgagcacaaa aaagaaacca ttaacacaag agcagcttga ggacgcacgt
     1381 cgccttaaag caatttatga aaaaaagaaa aatgaacttg gcttatccca ggaatctgtc
     1441 gcagacaaga tggggatggg gcagtcaggc gttggtgctt tatttaatgg catcaatgca
     1501 ttaaatgctt ataacgccgc attgcttgca aaaattctca aagttagcgt tgaagaattt
     1561 agcccttcaa tcgccagaga aatctacgag atgtatgaag cggttagtat gcagccgtca
     1621 cttagaagtg agtatgagta ccctgttttt tctcatgttc aggcagggat gttctcacct
     1681 gagcttagaa cctttaccaa aggtgatgcg gagagatggg taagcacaac caaaaaagcc
     1741 agtgattctg cattctggct tgaggttgaa ggtaattcca tgaccgcacc aacaggctcc
     1801 aagccaagct ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc tgttgagcca
     1861 ggtgatttct gcatagccag acttgggggt gatgagttta ccttcaagaa actgatcagg
     1921 gatagcggtc aggtgttttt acaaccacta aacccacagt acccaatgat cccatgcaat
     1981 gagagttgtt ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga gacgtttggc
     2041 gcagcaaacg acgaaaacta cgctttagca gcttgaGTTC ACTGCCGTAT AGGCAGTCGC
     2101 TGGGACGCCC GCCATGGTTC AGCCAAAAAA CTTAAGACCG CCGGTCTTGT CCACTACCTT
     2161 GCAGTAATGC GGTGGACAGG ATCGGCGGTT TTCTTTTCTC TTCTCAATTC TTCTGACCTG
     2221 TAACGAATAA TAGATAGTAA AGTAGTCTCC GATTGAGTTT TCTCTGCCGA GTCCCACCCA
     2281 GTTCTGTGAT TTCAGTAAGT TGGTAATTGA TACACTGTTG CGAGAACTGC TGCCTGGTAG
     2341 TAGATAGGTT GTTATTGAGT AAGAAGGTAA AGTGAACGAA ATCCCTGAAA CTGAGACTGT
     2401 AGAAAATAAG CTTCAGCCTG CGGTCCGGTT GACAGCTAGC TCAGTCCTAG GTATAATGCT
     2461 AGCTCGCTGG GACGCCCGGG GACTACACTT ACGAAACTAT TGATTGCTCA GCCTGCGGTC
     2521 CGGtaacacc gtgcgtgttg actattttac ctctggcggt gataatggtt gcTCGCTGGG
     2581 ACGCCCGTAA TTTTGTTTAA CTTTAAGAAG GAGATATACC atgaaaaagt ctttagccct
     2641 caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
     2701 cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta
     2761 tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
     2821 attcacctcg aaagcaagct gaCTCGGTAC CAAATTCCAG AAAAGAGGCC TCCCGAAAGG
     2881 GGGGCCTTTT TTCGTTTTGG TCCTAATAGA TAAAGGATAG GTCTGGTAGT GTTGTTCGTT
     2941 CTCGCAGGTA AATCAATAAT ACTCAGCAGT TCCGTAGACT TTTCAGTGGG ACAGGGTAGC
     3001 GATAACAGAT AGATTGTAAT AAGACACAGT AGGTGCTCGT AGTTGCGTGA AGAGAACCGC
     3061 TCAGGAAATC CAGTCAGAAG TATTGGTAAT CGTTGAAAAC TCAGTCGACT GCTGCCACCG
     3121 CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC
     3181 CAGGCATCAA ATAAAACGAA AGGCTCAGTC GGAAGACTGG GCCTTTCGTT TTATCTGTTG
     3241 TTTGTCGGTG AACGCTCTCC TGAGTAGGAC AAATCCGCCG GGAGCGGATT TGAACGTTGT
     3301 GAAGCAACGG CCCGGAGGGT GGCGGGCAGG ACGCCCGCCA TAAACTGCCA GGCATCAAAC
     3361 TAAGCAGAAG GCCATCCTGA CGGATGGCCT TTTTGCGTTT CAGATCTacc ggtaaaccag
     3421 caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcatcgtgg
     3481 ccggatcttg cggcccctcg gcttgaacga attgttagac attatttgcc gactaccttg
     3541 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
     3601 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
     3661 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
     3721 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
     3781 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
     3841 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
     3901 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
     3961 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
     4021 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
     4081 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
     4141 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc
     4201 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
     4261 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct catactcttc
     4321 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4381 gaatgtattt agaaaaataa acaaatagct agctcactcg gtcgctacgc tccgggcgtg
     4441 agactgcggc gggcgctgcg gacacataca aagttaccca cagattccgt ggataagcag
     4501 gggactaaca tgtgaggcaa aacagcaggg ccgcgccggt ggcgtttttc cataggctcc
     4561 gccctcctgc cagagttcac ataaacagac gcttttccgg tgcatctgtg ggagccgtga
     4621 ggctcaacca tgaatctgac agtacgggcg aaacccgaca ggacttaaag atccccaccg
     4681 tttccggcgg gtcgctccct cttgcgctct cctgttccga ccctgccgtt taccggatac
     4741 ctgttccgcc tttctccctt acgggaagtg tggcgctttc tcatagctca cacactggta
     4801 tctcggctcg gtgtaggtcg ttcgctccaa gctgggctgt aagcaagaac tccccgttca
     4861 gcccgactgc tgcgccttat ccggtaactg ttcacttgag tccaacccgg aaaagcacgg
     4921 taaaacgcca ctggcagcag ccattggtaa ctgggagttc gcagaggatt tgtttagcta
     4981 aacacgcggt tgctcttgaa gtgtgcgcca aagtccggct acactggaag gacagatttg
     5041 gttgctgtgc tctgcgaaag ccagttacca cggttaagca gttccccaac tgacttaacc
     5101 ttcgatcaaa ccacctcccc aggtggtttt ttcgtttaca gggcaaaaga ttacgcgcag
     5161 aaaaaaagga tctcaagaag atcctttgat cttttctact gaaccgctct agatttcagt
     5221 gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata agttgtaatt
     5281 ctcatgttag tcatgccccg cgcccaccgg aaggagctga ctgggttgCT CCTAGGATGC
     5341 AGTGACTCGc acatttcccc gaaaagtgcc acctGAATTC GAAATCCCTG AAACTGAGAC
     5401 TGTAGAAAAT
//