LOCUS       pHK-201                 5024 bp ds-DNA     circular     27-FEB-2025
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: 2 pCDF-MCS.gbk.txt
FEATURES             Location/Qualifiers
     CDS             complement(1..642)
                     /label="TetR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        813..871
                     /label="Ptet"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    917..931
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    936..941
                     /label="RBS"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    944..1690
                     /label="lambda repressor cI "
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1696..1710
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1732..1821
                     /label="ECK120029600 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2043..2077
                     /label="J23119"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    2138..2186
                     /label="cI binding site"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2223..2228
                     /label="RBS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             2235..2456
                     /label="M13 gene VIII"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2457..2517
                     /label="L3S2P21 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     gene            complement(3139..3927)
                     /label="SpecR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     oriT            complement(4067..4805)
                     /label="CDF ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 ttaggaccca ctttcacatt taagttgttt ttctaatccg catataatca attcaaggcc
       61 gaataagaag gctggctctg caccttggtg atcaaataat tcgatagctt gtcgtaataa
      121 tggcggcata ctatcagtag taggtgtttc cctttcttct ttagcgactt gatgctcttg
      181 atcttccaat acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat ataatgcatt
      241 ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga ttttcgagag tttcatactg
      301 tttttctgta ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc
      361 acatctaaaa cttttagcgt tattacgtaa aaaatcttgc cagctttccc cttctaaagg
      421 gcaaaagtga gtatggtgcc tatctaacat ctcaatggct aaggcgtcga gcaaagcccg
      481 cttatttttt acatgccaat acaatgtagg ctgctctaca cctagcttct gggcgagttt
      541 acgggttgtt aaaccttcga ttccgacctc attaagcagc tctaatgcgc tgttaatcac
      601 tttactttta tctaatctgg aCATTTTTTT CCTCCTGGAT CCttaatttt taaagtatgg
      661 gcaatcaatt gctcctgtta aaattgcttt agaaatactt tggcagcggt ttgttgtatt
      721 gagtttcatt tgcgcattgg ttaaatggaa agtgacagta cgctcactgc gaattcGAGt
      781 TGTaAAACGA CGGCCAgTAC TCCACCGTTG GCttttttcc ctatcagtga tagagattga
      841 catccctatc agtgatagag ataatgagca cAAGAGATTT CTACACGATT GAGCACTGTC
      901 TCAGCCTGCG GTCCGGCTGC CGTATAGGCA Gaaagaggag aaaatgagca caaaaaagaa
      961 accattaaca caagagcagc ttgaggacgc acgtcgcctt aaagcaattt atgaaaaaaa
     1021 gaaaaatgaa cttggcttat cccaggaatc tgtcgcagac aagatgggga tggggcagtc
     1081 aggcgttggt gctttattta atggcatcaa tgcattaaat gcttataacg ccgcattgct
     1141 tgcaaaaatt ctcaaagtta gcgttgaaga atttagccct tcaatcgcca gagaaatcta
     1201 cgagatgtat gaagcggtta gtatgcagcc gtcacttaga agtgagtatg agtaccctgt
     1261 tttttctcat gttcaggcag ggatgttctc acctgagctt agaaccttta ccaaaggtga
     1321 tgcggagaga tgggtaagca caaccaaaaa agccagtgat tctgcattct ggcttgaggt
     1381 tgaaggtaat tccatgaccg caccaacagg ctccaagcca agctttcctg acggaatgtt
     1441 aattctcgtt gaccctgagc aggctgttga gccaggtgat ttctgcatag ccagacttgg
     1501 gggtgatgag tttaccttca agaaactgat cagggatagc ggtcaggtgt ttttacaacc
     1561 actaaaccca cagtacccaa tgatcccatg caatgagagt tgttccgttg tggggaaagt
     1621 tatcgctagt cagtggcctg aagagacgtt tggcgcagca aacgacgaaa actacgcttt
     1681 agcagcttga GTTCACTGCC GTATAGGCAG TCGCTGGGAC GCCCGCCATG GTTCAGCCAA
     1741 AAAACTTAAG ACCGCCGGTC TTGTCCACTA CCTTGCAGTA ATGCGGTGGA CAGGATCGGC
     1801 GGTTTTCTTT TCTCTTCTCA ATTCTTCTGA CCTGTAACGA ATAATAGATA GTAAAGTAGT
     1861 CTCCGATTGA GTTTTCTCTG CCGAGTCCCA CCCAGTTCTG TGATTTCAGT AAGTTGGTAA
     1921 TTGATACACT GTTGCGAGAA CTGCTGCCTG GTAGTAGATA GGTTGTTATT GAGTAAGAAG
     1981 GTAAAGTGAA CGAAATCCCT GAAACTGAGA CTGTAGAAAA TAAGCTTCAG CCTGCGGTCC
     2041 GGTTGACAGC TAGCTCAGTC CTAGGTATAA TGCTAGCTCG CTGGGACGCC CGGGGACTAC
     2101 ACTTACGAAA CTATTGATTG CTCAGCCTGC GGTCCGGtaa caccgtgcgt gttgactatt
     2161 ttacctctgg cggtgataat ggttgcTCGC TGGGACGCCC GTAATTTTGT TTAACTTTAA
     2221 GAAGGAGATA TACCatgaaa aagtctttag ccctcaaagc ctctgtagcc gttgctaccc
     2281 tcgttccgat gctgtctttc gctgctgagg gtgacgatcc cgcaaaagcg gcctttaact
     2341 ccctgcaagc ctcagcgacc gaatatatcg gttatgcgtg ggcgatggtt gttgtcattg
     2401 tcggcgcaac tatcggtatc aagctgttta agaaattcac ctcgaaagca agctgaCTCG
     2461 GTACCAAATT CCAGAAAAGA GGCCTCCCGA AAGGGGGGCC TTTTTTCGTT TTGGTCCTAA
     2521 TAGATAAAGG ATAGGTCTGG TAGTGTTGTT CGTTCTCGCA GGTAAATCAA TAATACTCAG
     2581 CAGTTCCGTA GACTTTTCAG TGGGACAGGG TAGCGATAAC AGATAGATTG TAATAAGACA
     2641 CAGTAGGTGC TCGTAGTTGC GTGAAGAGAA CCGCTCAGGA AATCCAGTCA GAAGTATTGG
     2701 TAATCGTTGA AAACTCAGTC GACTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT
     2761 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCCAGGCA TCAAATAAAA CGAAAGGCTC
     2821 AGTCGGAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT CTCCTGAGTA
     2881 GGACAAATCC GCCGGGAGCG GATTTGAACG TTGTGAAGCA ACGGCCCGGA GGGTGGCGGG
     2941 CAGGACGCCC GCCATAAACT GCCAGGCATC AAACTAAGCA GAAGGCCATC CTGACGGATG
     3001 GCCTTTTTGC GTTTCAGATC Taccggtaaa ccagcaatag acataagcgg ctatttaacg
     3061 accctgccct gaaccgacga ccgggtcatc gtggccggat cttgcggccc ctcggcttga
     3121 acgaattgtt agacattatt tgccgactac cttggtgatc tcgcctttca cgtagtggac
     3181 aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc
     3241 ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc
     3301 ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa
     3361 cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa
     3421 ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc
     3481 cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc
     3541 aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc
     3601 aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat
     3661 ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag
     3721 gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa
     3781 atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc
     3841 cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga
     3901 tacttcggcg atcaccgctt ccctcatact cttccttttt caatattatt gaagcattta
     3961 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     4021 agctagctca ctcggtcgct acgctccggg cgtgagactg cggcgggcgc tgcggacaca
     4081 tacaaagtta cccacagatt ccgtggataa gcaggggact aacatgtgag gcaaaacagc
     4141 agggccgcgc cggtggcgtt tttccatagg ctccgccctc ctgccagagt tcacataaac
     4201 agacgctttt ccggtgcatc tgtgggagcc gtgaggctca accatgaatc tgacagtacg
     4261 ggcgaaaccc gacaggactt aaagatcccc accgtttccg gcgggtcgct ccctcttgcg
     4321 ctctcctgtt ccgaccctgc cgtttaccgg atacctgttc cgcctttctc ccttacggga
     4381 agtgtggcgc tttctcatag ctcacacact ggtatctcgg ctcggtgtag gtcgttcgct
     4441 ccaagctggg ctgtaagcaa gaactccccg ttcagcccga ctgctgcgcc ttatccggta
     4501 actgttcact tgagtccaac ccggaaaagc acggtaaaac gccactggca gcagccattg
     4561 gtaactggga gttcgcagag gatttgttta gctaaacacg cggttgctct tgaagtgtgc
     4621 gccaaagtcc ggctacactg gaaggacaga tttggttgct gtgctctgcg aaagccagtt
     4681 accacggtta agcagttccc caactgactt aaccttcgat caaaccacct ccccaggtgg
     4741 ttttttcgtt tacagggcaa aagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4801 tgatcttttc tactgaaccg ctctagattt cagtgcaatt tatctcttca aatgtagcac
     4861 ctgaagtcag ccccatacga tataagttgt aattctcatg ttagtcatgc cccgcgccca
     4921 ccggaaggag ctgactgggt tgCTCCTAGG ATGCAGTGAC TCGcacattt ccccgaaaag
     4981 tgccacctGA ATTCGAAATC CCTGAAACTG AGACTGTAGA AAAT
//