LOCUS       pHK-001.23              4641 bp ds-DNA     circular     27-FEB-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    61..150
                     /label="ECK120029600 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    372..406
                     /label="P(BBa_J23100)"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(467..489)
                     /label="bs-2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    561..575
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    597..602
                     /label="RBS"
                     /ApEinfo_revcolor="#fdc68a"
                     /ApEinfo_fwdcolor="#fdc68a"
     gene            610..1329
                     /label="sfGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1330..1344
                     /label="Suffix"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1353..1413
                     /label="L3S2P21 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1635..1669
                     /label="J23119"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(1730..1752)
                     /label="bs-3"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1818..1832
                     /label="Csy4 minimal cut motif"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1854..1859
                     /label="RBS"
                     /ApEinfo_revcolor="#fdc68a"
                     /ApEinfo_fwdcolor="#fdc68a"
     gene            1867..2574
                     /label="mCherry"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2598..2654
                     /label="ECK120033737 Terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     gene            complement(3071..3886)
                     /label="KanR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     oriT            3996..4631
                     /label="ColA ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 GAATTCATAG GATAGATTCT GGAAACTTTA CCGTCCGAGC TCAGGCTTAC CTTACCATGG
       61 TTCAGCCAAA AAACTTAAGA CCGCCGGTCT TGTCCACTAC CTTGCAGTAA TGCGGTGGAC
      121 AGGATCGGCG GTTTTCTTTT CTCTTCTCAA TTCTTCTGAC CTGTAACGAA TAATAGATAG
      181 TAAAGTAGTC TCCGATTGAG TTTTCTCTGC CGAGTCCCAC CCAGTTCTGT GATTTCAGTA
      241 AGTTGGTAAT TGATACACTG TTGCGAGAAC TGCTGCCTGG TAGTAGATAG GTTGTTATTG
      301 AGTAAGAAGG TAAAGTGAAC GAAATCCCTG AAACTGAGAC TGTAGAAAAT AAGCTTCAGC
      361 CTGCGGTCCG Gttgacggct agctcagtcc taggtacagt gctagcTCGC TGGGACGCCC
      421 GAGATAGCCG TTACACAGGT GACACTTATT TCAGCCTGCG GTCCGGccaA GTACTTAGTA
      481 CACACTGATT CGCTGGGACG CCCGGGATCC AAGAGATTTC TACACGATTG AGCACTGTCT
      541 CAGCCTGCGG TCCGGGTTCA CTGCCGTATA GGCAGTAATT TTGTTTAACT TTAAGAAGGA
      601 GATATACATA TGCGTAAAGG CGAAGAACTG TTTACCGGTG TGGTTCCGAT TCTGGTGGAA
      661 CTGGACGGCG ATGTTAATGG TCATAAATTC AGTGTTCGCG GCGAAGGTGA AGGCGATGCG
      721 ACGAACGGCA AACTGACCCT GAAATTTATC TGCACCACGG GTAAACTGCC GGTCCCGTGG
      781 CCGACGCTGG TGACCACGCT GACCTATGGC GTTCAATGTT TTGCGCGTTA CCCGGATCAC
      841 ATGAAACAGC ACGACTTTTT CAAATCGGCC ATGCCGGAAG GCTATGTGCA GGAACGTACG
      901 ATTAGCTTTA AAGACGATGG TACGTATAAA ACCCGCGCGG AAGTGAAATT CGAAGGCGAT
      961 ACCCTGGTTA ACCGTATCGA ACTGAAAGGT ATCGATTTCA AAGAAGACGG CAATATTCTG
     1021 GGTCATAAAC TGGAATATAA CTTCAATTCC CACAACGTGT ACATCACCGC GGATAAACAG
     1081 AAAAACGGCA TTAAAGCCAA TTTCAAAATC CGCCATAATG TGGAAGATGG TAGCGTTCAG
     1141 CTGGCCGACC ACTATCAGCA AAACACGCCG ATTGGTGATG GCCCGGTCCT GCTGCCGGAC
     1201 AATCACTACC TGAGTACCCA GTCCGTGCTG TCAAAAGATC CGAACGAAAA ACGTGACCAC
     1261 ATGGTCCTGC TGGAATTTGT GACGGCTGCG GGTATCACCC ACGGCATGGA CGAACTGTAT
     1321 AAATAATAAT CGCTGGGACG CCCGCCTGCA GGCTCGGTAC CAAATTCCAG AAAAGAGGCC
     1381 TCCCGAAAGG GGGGCCTTTT TTCGTTTTGG TCCTAATAGA TAAAGGATAG GTCTGGTAGT
     1441 GTTGTTCGTT CTCGCAGGTA AATCAATAAT ACTCAGCAGT TCCGTAGACT TTTCAGTGGG
     1501 ACAGGGTAGC GATAACAGAT AGATTGTAAT AAGACACAGT AGGTGCTCGT AGTTGCGTGA
     1561 AGAGAACCGC TCAGGAAATC CAGTCAGAAG TATTGGTAAT CGTTGAAAAC TCAGTCGACC
     1621 AGCCTGCGGT CCGGTTGACA GCTAGCTCAG TCCTAGGTAT AATGCTAGCT CGCTGGGACG
     1681 CCCGGTAGTG CTTATCAGAC CCAATACTGT TGAACAGCCT GCGGTCCGGc caAGAGTACA
     1741 CTAGCTCAGT CATCGCTGGG ACGCCCGTAG AACTCTGTGA GGATAAAGTC TCCCTTACAG
     1801 CCTGCGGTCC GGGTTCACTG CCGTATAGGC AGTAATTTTG TTTAACTTTA AGAAGGAGAT
     1861 ATACATATGG TGAGCAAGGG CGAGGAGGAT AACATGGCCA TCATCAAGGA GTTCATGCGC
     1921 TTCAAGGTGC ACATGGAGGG CTCCGTGAAC GGCCACGAGT TCGAGATCGA GGGCGAGGGC
     1981 GAGGGCCGCC CCTACGAGGG CACCCAGACC GCCAAGCTGA AGGTGACCAA GGGTGGCCCC
     2041 CTGCCCTTCG CCTGGGACAT CCTGTCCCCT CAGTTCATGT ACGGCTCCAA GGCCTACGTG
     2101 AAGCACCCCG CCGACATCCC CGACTACTTG AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG
     2161 GAGCGCGTGA TGAACTTCGA GGACGGCGGC GTGGTGACCG TGACCCAGGA CTCCTCtCTG
     2221 CAGGACGGCG AGTTCATCTA CAAGGTGAAG CTGCGCGGCA CCAACTTCCC CTCCGACGGC
     2281 CCCGTAATGC AGAAGAAGAC tATGGGCTGG GAGGCCTCCT CCGAGCGGAT GTACCCCGAG
     2341 GACGGCGCgC TGAAGGGCGA GATCAAGCAG AGGCTGAAGC TGAAGGACGG CGGCCACTAC
     2401 GACGCTGAaG TCAAGACCAC CTACAAGGCC AAGAAGCCCG TGCAGCTGCC CGGCGCgTAC
     2461 AACGTCAACA TCAAGTTGGA CATCACCTCC CACAACGAGG ACTACACCAT CGTGGAACAG
     2521 TACGAACGCG CCGAGGGCCG CCACTCCACC GGCGGCATGG ACGAGCTGTA CAAGTCGCTG
     2581 GGACGCCCGG CGGCCGCgga aacacagAAA AAAGCCCGCA CCTGACAGTG CGGGCTTTTT
     2641 TTTTcgacca aaggGCGAAC GACGAGTCAC TGTTGAGGAT AAATACTTTC TCTACTAGGC
     2701 GCCTGTTACA CAGGTCCTCA GCGGCGCGCC TTTGTCGGTG AACGCTCTCC TGAGTAGGAC
     2761 AAATCCGCCG GGAGCGGATT TGAACGTTGT GAAGCAACGG CCCGGAGGGT GGCGGGCAGG
     2821 ACGCCCGCCA TAAACTGCCA GGCATCAAAC TAAGCAGAAG GCCATCCTGA CGGATGGCCT
     2881 TTTTGCGTTT CAGATCTACC GGTaaaccag caatagacat aagcggctat ttaacgaccc
     2941 tgccctgaac cgacgacaag ctgacgaccg ggtctccgca agtggcactt ttcggggaaa
     3001 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3061 gaattaattc ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag
     3121 gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga
     3181 ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat
     3241 caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat
     3301 gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt
     3361 caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca
     3421 ttcgtgattg cgcctgagcg agacgaaata cgcggtcgct gttaaaagga caattacaaa
     3481 caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg
     3541 aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta
     3601 accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg
     3661 tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat
     3721 gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg
     3781 attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat
     3841 ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg gctcatactc ttcctttttc
     3901 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     3961 tttagaaaaa taaacaaata ggcatgctag cgcagaaacg tcctagaaga tgccaggagg
     4021 atacttagca gagagacaat aaggccggag cgaagccgtt tttccatagg ctccgccccc
     4081 ctgacgaaca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat
     4141 aaagatacca ggcgtttccc cctgatggct ccctcttgcg ctctcctgtt cccgtcctgc
     4201 ggcgtccgtg ttgtggtgga ggctttaccc aaatcaccac gtcccgttcc gtgtagacag
     4261 ttcgctccaa gctgggctgt gtgcaagaac cccccgttca gcccgactgc tgcgccttat
     4321 ccggtaacta tcatcttgag tccaacccgg aaagacacga caaaacgcca ctggcagcag
     4381 ccattggtaa ctgagaatta gtggatttag atatcgagag tcttgaagtg gtggcctaac
     4441 agaggctaca ctgaaaggac agtatttggt atctgcgctc cactaaagcc agttaccagg
     4501 ttaagcagtt ccccaactga cttaaccttc gatcaaaccg cctccccagg cggttttttc
     4561 gtttacagag caggagatta cgacgatcgt aaaaggatct caagaagatc ctttacggat
     4621 tcccgacacc atcactctag a
//