LOCUS       Exported                8944 bp ds-DNA     linear   SYN 28-FEB-2025
DEFINITION  PB_CAG_Tir1_Bsd LOCUS       New_DNA                 8944 bp ds-DNA  
              circular     07-JAN-2016.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 28, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8944
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
       61 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      121 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      181 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      241 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
      301 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
      361 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
      421 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      481 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      541 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      601 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      661 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      721 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
      781 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
      841 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
      901 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
      961 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1021 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     1081 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     1141 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     1201 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     1261 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     1321 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     1381 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1441 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1501 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1561 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1621 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1681 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1741 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1801 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1861 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1921 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1981 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2041 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2101 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2161 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2221 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2281 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2341 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     2401 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     2461 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     2521 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     2581 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     2641 tgggtacctt aaccctagaa agatagtctg cgtaaaattg acgcatgcat tcttgaaata
     2701 ttgctctctc tttctaaata gcgcgaatcc gtcgctgtgc atttaggaca tctcagtcgc
     2761 cgcttggagc tcccgtgagg cgtgcttgtc aatgcggtaa gtgtcactga ttttgaacta
     2821 taacgaccgc gtgagtcaaa atgacgcatg attatctttt acgtgacttt taagatttaa
     2881 ctcatacgat aattatattg ttatttcatg ttctacttac gtgataactt attatatata
     2941 tattttcttg ttatagatat cataacttcg tataatgtat gctatacgaa gttatgcggc
     3001 cctcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     3061 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     3121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     3181 gggactttcc attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta
     3241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     3301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     3361 gtattagtca tcgctattac catgggtcga ggtgagcccc acgttctgct tcactctccc
     3421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     3481 agcgatgggg gcgggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
     3541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
     3601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
     3661 cgggagtcgc tgcgttgcct tcgccccgtg ccccgctccg cgccgcctcg cgccgcccgc
     3721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
     3781 cgggctgtaa ttagcgcttg gtttaatgac ggctcgtttc ttttctgtgg ctgcgtgaaa
     3841 gccttaaagg gctccgggag ggccctttgt gcggggggga gcggctcggg gggtgcgtgc
     3901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggcccgcgct gcccggcggc tgtgagcgct
     3961 gcgggcgcgg cgcggggctt tgtgcgctcc gcgtgtgcgc gaggggagcg cggccggggg
     4021 cggtgccccg cggtgcgggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     4081 gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc
     4141 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg
     4201 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     4261 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggccccggag cgccggcggc
     4321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     4381 ggacttcctt tgtcccaaat ctggcggagc cgaaatctgg gaggcgccgc cgcaccccct
     4441 ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc
     4501 ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg
     4561 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     4621 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     4681 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattcctc gaatcaacaa
     4741 gtttgtacaa aaaagcaggc tacccgccgc caccatgggc cccgccgccc ccaagaagaa
     4801 gagaaaggtg ctggacacgt acttccctga agaagttgtg gaacatatct tttcttttct
     4861 tcctgcccaa agagaccgga atacagtctc tttggtatgt aaggtttggt atgagattga
     4921 aaggttgtca aggagaggag tgtttgtggg aaactgctat gcggtaaggg ctggaagagt
     4981 cgctgccaga ttccccaatg tgagggcact tacagttaag ggtaaaccac attttgctga
     5041 tttcaatttg gttcctccgg actggggagg ttatgctgga ccatggattg aggctgctgc
     5101 cagaggttgt catgggttgg aggagttgag gatgaagagg atggttgtgt ctgatgagtc
     5161 ccttgaactt ctggcccggt ccttccccag attcagagct ttggtcttga tttcttgtga
     5221 gggtttctct actgacggac tcgctgcagt cgcttcccac tgtaagttgt tgagggagtt
     5281 ggacctccaa gaaaatgagg ttgaggacag aggaccaaga tggttgtcat gtttccctga
     5341 ctcatgtact tcccttgttt ccttgaattt cgcgtgtatc aaaggagagg tcaatgccgg
     5401 gagtcttgag agattggtct caagaagtcc caacttgcga tcactgaggc tcaacagatc
     5461 tgtgagtgtg gataccttgg caaagattct cttgagaacc cccaatttgg aggaccttgg
     5521 aactgggaat ctcacggatg acttccaaac tgagtcttac tttaagttga ctagtgcttt
     5581 ggaaaagtgt aagatgttga gatcactgtc cggattctgg gatgcttcac ctgtttgtct
     5641 ttccttcatc taccctcttt gtgcccagct gactggactc aacttgtcct atgctccaac
     5701 actggatgcg agtgacttga ccaagatgat ttcaagatgc gtgaagcttc aaagactttg
     5761 ggtcctcgat tgtatctccg acaaaggact tcaagtggtg gcatcatctt gtaaggatct
     5821 ccaagagttg agagtgtttc caagtgactt ctatgtggca ggttacagtg ctgtaactga
     5881 ggaagggttg gttgctgtct cccttggatg tcccaagttg aattcactct tgtacttttg
     5941 tcaccaaatg actaacgctg cccttgtgac agtggccaaa aattgcccta acttcacacg
     6001 cttcagactt tgtatccttg aacctgggaa acctgacgtg gtcaccagtc agcccttgga
     6061 cgaaggattt ggggcgattg tgagagaatg taagggactc caaagactct ctatcagtgg
     6121 actcctgact gacaaggtgt ttatgtacat tggtaagtac gctaagcagc tggaaatgct
     6181 gtctattgcc tttgccggtg attctgacaa ggggatgatg catgttatga atggatgtaa
     6241 gaacttgaga aagcttgaga taagagacag tccattcgga gatgcagccc tccttggtaa
     6301 cttcgcaaga tacgaaacta tgaggtctct ttggatgtcc agctgtaatg ttacactcaa
     6361 gggatgtcaa gtgctggcct caaaaatgcc aatgctgaat gtggaggtga tcaacgagag
     6421 agatggatcc aatgaaatgg aagagaacca cggagatctt cccaaggtgg aaaagttgta
     6481 tgtctacaga accactgctg gggcaaggga tgacgctcca aacttcgtga aaatactgtg
     6541 aacccagctt tcttgtacaa agtggttgat tcgaggccgc tcgataagct tgatatcgaa
     6601 ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caactggctc
     6661 acaaatacca ctgagatcga cggtatcgat aagcttgata tcgaattccg cccccccccc
     6721 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     6781 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     6841 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     6901 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     6961 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     7021 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     7081 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     7141 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     7201 atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac
     7261 gtggttttcc tttgaaaaac acgatgataa tatggccaca accatgcctt tgtctcaaga
     7321 agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga
     7381 ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt
     7441 atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc
     7501 ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag
     7561 cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt
     7621 gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta
     7681 tgtgtgggag ggttaactag ataagtaatg atcataatca gccatatcac atctgtagag
     7741 gttttacttg ctttgtccga cggcggccca cgggtcccag gggggtcgat ctttttccct
     7801 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     7861 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     7921 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     7981 catatgccat atgctggctg ccatgaacaa aggtggctat aaagaggtca tcagtatatg
     8041 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
     8101 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
     8161 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
     8221 ctcttatgaa gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt
     8281 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8341 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8401 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcggccgcg tcgagataac
     8461 ttcgtataat gtatgctata cgaagttatg ttttgttact ttatagaaga aattttgagt
     8521 ttttgttttt ttttaataaa taaataaaca taaataaatt gtttgttgaa tttattatta
     8581 gtatgtaagt gtaaatataa taaaacttaa tatctattca aattaataaa taaacctcga
     8641 tatacagacc gataaaacac atgcgtcaat tttacgcatg attatcttta acgtacgtca
     8701 caatatgatt atctttctag ggttaaagag ctccaattcg ccctatagtg agtcgtatta
     8761 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     8821 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     8881 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     8941 cggc
//
