LOCUS       Exported                8412 bp ds-DNA     linear   SYN 28-FEB-2025
DEFINITION  pBrg1-GGSG_AID_3xF_PP_Target2 Reverse Complement DNA Sequence 
            pBluescript II SK.seq(1,2961).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 28, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccggg ccccccctcg
     2221 aggtcgacgg tatcgataag cttgatatcg gcataggctc caacctccac gggcccatgc
     2281 ccacacacag acataacgct acataagtaa aagtaaaagt aagccaggta tgatggttca
     2341 tggacagctt cattcccggc agtcaggagg caaaggcagg ctctcttaag ttttaggctg
     2401 gcctggtaca catagcaagt tccaggccag ccagggctat atagagaccc taatttttta
     2461 gagggctttc ttgtttattt tgcctgcatg tatggatgtg tgccactgtg tgcctggtga
     2521 tcgtggcggc tagaagaacg cattggatcc cctggaactg gagttccagg gtgttatgtg
     2581 aacacccact atgtgggttc tcaggagtga attcgtgtcc tttgtaagag cagccagttt
     2641 tcttagctat tgagtcgtct ctccaaccct aaaaatgact cttaataaga taatggctgc
     2701 aggcatagta gtaaactccc gtgatcccag gccttgggag gtagaagccg gaagatcagg
     2761 aggcgttcat gaccatcctt ggttgcctag caagtttgag gccagcctga gctctattag
     2821 accaaaatgg cagtcagaag acatgttcat actcaagaat ggctataatg agtggctgcc
     2881 tcttatgggg ttccatgttg gcctgtccct accatggtgc agggcaaggt ccaagttttg
     2941 atgcagtttt cttttttttt ttttcaaaga ttcatttatt tatttattat atgtaagtat
     3001 actgtagctg tcttcagaca ctccagaaga gggagtcaga tctcgttaag gatggttgtg
     3061 agccactatg tggttgctgg gatttgaacc caggaccttt ggaagagtaa tcagtgctct
     3121 taactgctga gtcgtcttgc cagcctcctt gatgcagttt tttttttttt gccacagtaa
     3181 gataatttat cccttaagga tctaggccct tccacagcat tgagaacacc atggacaagg
     3241 gagagtgcct aggccgcagc ctactcacaa actattcccc ctgttctttc aggaccgctc
     3301 aggaagtggc agtgaggaag acggctctgg tggcggtggc tctggcggag gtgggtccgg
     3361 tggcggcgga agcgccccag gaatgggcag tgtcgagctg aatctgaggg agactgagct
     3421 gtgtcttggt cttcccggtg gagatacagt ggctccggta accggaaaca agagagggtt
     3481 ctcagagacg gttgatctga agctaaatct gaataatgag cctgcaaaca aggaaggatc
     3541 tacgactcat gacgtcgtga cttttgattc caaggagaag agtgcttgtc ctaaagatcc
     3601 agccaaacct ccggccaagg cacaagttgt gggatggcca ccggtgagat cataccggaa
     3661 gaacgtgatg gtttcctgcc aaaaatcaag cggtggcccg gaggcggcgg cgttcgtgaa
     3721 ggtatcaatg gacggagcac cgtacttgag gaaaatcgat ttgaggatgt ataaaagcta
     3781 cgatgagctt tctaatgctt tgtccaacat gttcagctct tttaccatgg gcaaacatgg
     3841 aggagaagaa ggaatgatag acttcatgaa tgagaggaaa ttgatggatt tggtgaatag
     3901 ctgggactat gttccctctt atgaagacaa agacggtgat tggatgctcg tcggcgacgt
     3961 tccttggcca atgttcgtcg atacatgcaa gcgtttacgt ctcatgaaag gatcggatgc
     4021 cattggtctc gctccgaggg cgatggagaa gtgcaagagc agagctgact acaaagacca
     4081 tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca agtagaagct
     4141 tcgatcggta ccaaggcgca taacgatacc acgatatcaa caagtttgta caaaaaagca
     4201 ggctggcgcc ggaaccaatt cagtcgacgt ttaaacgatc gtaactttaa ataatgccaa
     4261 ttatttaaag ttacatatga gaaagtatag gaacttcagc ttgatatcga attaattcta
     4321 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
     4381 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
     4441 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
     4501 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
     4561 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
     4621 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
     4681 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc
     4741 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
     4801 ttcctcatct ccgggccttt cgacctgcag ccaacgccac catggggacc gagtacaagc
     4861 ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg
     4921 cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg
     4981 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     5041 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     5101 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     5161 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     5221 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     5281 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     5341 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     5401 aaggaccgcg cacctggtgc atgacccgca agcccggtgc cggatccatg cccacgctac
     5461 tgcgggttta tatagacggt cctcacggga tggggaaaac caccaccacg caactgctgg
     5521 tggccctggg ttcgcgcgac gatatcgtct acgtacccga gccgatgact tactggcagg
     5581 tgctgggggc ttccgagaca atcgcgaaca tctacaccac acaacaccgc ctcgaccagg
     5641 gtgagatatc ggccggggac gcggcggtgg taatgacaag cgcccagata acaatgggca
     5701 tgccttatgc cgtgaccgac gccgttctgg ctcctcatat cgggggggag gctgggagct
     5761 cacatgcccc gcccccggcc ctcaccctca tcttcgaccg ccatcccatc gccgccctcc
     5821 tgtgctaccc ggccgcgcga taccttatgg gcagcatgac cccccaggcc gtgctggcgt
     5881 tcgtggccct catcccgccg accttgcccg gcacaaacat cgtgttgggg gcccttccgg
     5941 aggacagaca catcgaccgc ctggccaaac gccagcgccc cggcgagcgg cttgacctgg
     6001 ctatgctggc cgcgattcgc cgcgtttacg ggctgcttgc caatacggtg cggtatctgc
     6061 agggcggcgg gtcgtggcgg gaggattggg gacagctttc ggggacggcc gtgccgcccc
     6121 agggtgccga gccccagagc aacgcgggcc cacgacccca tatcggggac acgttattta
     6181 ccctgtttcg ggcccccgag ttgctggccc ccaacggcga cctgtacaac gtgtttgcct
     6241 gggccttgga cgtcttggcc aaacgcctcc gtcccatgca cgtctttatc ctggattacg
     6301 accaatcgcc cgccggctgc cgggacgccc tgctgcaact tacctccggg atggtccaga
     6361 cccacgtcac cacccccggc tccataccga cgatctgcga cctggcgcgc acgtttgccc
     6421 gggagatggg ggaggctaac tgagctctag agctcgctga tcagcctcga ctgtgccttc
     6481 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     6541 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     6601 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     6661 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     6721 gggctcgaca tactagagta cttaacttta aataatgcca attatttaaa gttaggcgcg
     6781 ccgatatcta gacccagctt ctagttctag agcagacatt cctgagtcct gaccccgagg
     6841 cgctcgtccc agccaagatg gagtagccct tagcagtgat gggtagcacc agatgtagtt
     6901 tcgaacttgg agaactgtac acatgcaatc ttccacattt ttaggcagag aagtataggc
     6961 ctgtctgtcg gccctggcct ggcctcgagt ctctaccagc attaactgtc tagagagggg
     7021 acctcctggg agcaccatcc acctccccag gccccagtca ctgtagctca gtggatgcat
     7081 gcgcgtgccg gccgctcctt gtactgtatc ttactggaca gggccagctc tccaggaggc
     7141 tcacaggccc agcgggtatg tcagtgtcac tggagtcaga cagtaataaa ttaaagcaat
     7201 gacaagccac cactggctcc ctggactcct tgctgtcagc agtggctccg gggccacaga
     7261 gaagaaagaa agacttttag gaactgggtc taacttatgg gcaaagtact tgccttgcca
     7321 ggtgtatggg ttttgcattc ccatcaccca cacaccctaa acaagccaag tcagtgagct
     7381 tcaagttaga gcctccacct caatgtgtac gtggaaagca atcaaagatg atgcctagca
     7441 tccacctctg gccctcatgt gcagatgtac acacactgaa ttacatacac gggacacaca
     7501 catccacacg gaggcagtcc atgacttgca ctggggagat ggtaccatag gcgaaagtgc
     7561 cacaggcaca gggccaggct aatttagtcc tgcagtcctg tgctcttaag atgaaggcac
     7621 aaagaggaac cccaggcgct ccaactagca tgccaggcag tgacaagacc ctgcttcaaa
     7681 tgaatcagag cccacattca gtattgccct cttacccgat gcgatgccca tgccctcaca
     7741 tagccaccgc ggtggagctc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     7801 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     7861 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     7921 tcccaacagt tgcgcagcct gaatggcgaa tggaaattgt aagcgttaat attttgttaa
     7981 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     8041 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     8101 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     8161 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     8221 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     8281 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     8341 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     8401 agggcgcgtc ag
//
