LOCUS       Exported                9280 bp ds-DNA     circular SYN 27-FEB-2025
DEFINITION  Dox-inducible expression of the engineered catalytic-domain (CD) of 
            epigenetic effector Ring1b fused with scFV-sfGFP to programme 
            H2AK119 ubiquitination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Policarpi C, Munafo M, Tsagkris S, Carlini V, Hackett JA.
  TITLE     Systematic epigenome editing captures the context-dependent 
            instructive function of chromatin modifications.
  JOURNAL   Nat Genet 2024 Jun;56:1168-1180
  PUBMED    38724747
REFERENCE   2  (bases 1 to 9280)
  AUTHORS   Epitome Tx
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 28, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Under patent. Not for commercial usage or modification.
FEATURES             Location/Qualifiers
     source          1..9280
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(870..1104)
                     /label=Piggy 3'
     promoter        1386..1764
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    1394..1412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1430..1448
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1466..1484
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1502..1520
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1538..1556
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1574..1592
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1610..1628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1638..1658
                     /label=OJH_513 Seq_TRE3G-scFv
     primer_bind     1763..1798
                     /label=Fragment 1.FOR (2)
     CDS             1780..2481
                     /codon_start=1
                     /label=Recoded scFV
                     /translation="MGPDIVMTQSPSSLSASVGDRVTITCRSSTGAVTTSNYASWVQEK
                     PGKLFKGLIGGTNNRAPGVPSRFSGSLIGDKATLTISSLQPEDFATYFCALWYSNHWVF
                     GQGTKVELKRGGGGSGGGGSGGGGSSGGGSEVKLLESGGGLVQPGGSLKLSCAVSGFSL
                     TDYGVNWVRQAPGRGLEWIGVIWGDGITDYNSALKDRFIISKDNGKNTVYLQMSKVRSD
                     DTALYYCVTGLF"
     primer_bind     complement(2171..2190)
                     /label=scFV-recodedgenot_R
     CDS             2521..2547
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2548..2637
                     /codon_start=1
                     /label=Linker
                     /translation="GGGGGSGGGGSGGGGSGGGGSLDPGGGGSG"
     CDS             2638..3348
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(2939..2958)
                     /label=OJH_757_sfGFP_exp_R
     primer_bind     3193..3213
                     /label=OJH_511_Seq_sfGFP-effector
     CDS             3367..3387
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3394..3414
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3427..3462
                     /label=Linker
     primer_bind     complement(3447..3468)
                     /label=Fragment 1.REV (2)
     primer_bind     3449..3491
                     /label=p163A_RING1B_cloning_F
     CDS             3469..4065
                     /label=Ring1b CD
     primer_bind     complement(3596..3636)
                     /label=Hifi_cat_mut_RING1B_R
     primer_bind     3617..3656
                     /label=Hifi_cat_mut_RING1B_F
     misc_feature    3622..3624
                     /label=I53S for catalytic mutant
     primer_bind     complement(3884..3903)
                     /label=p163A_RING1B_R1
     primer_bind     complement(4040..4083)
                     /label=p163A_RING1B_cloning_R
     misc_feature    4066..4068
                     /label=STOP CODON insertion
     primer_bind     4069..4104
                     /label=Fragment 2.FOR
     misc_feature    4072..5847
     primer_bind     complement(4177..4194)
                     /label=Fragment 2.REV
     polyA_signal    4180..4301
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    4312..5847
     misc_feature    4312..5847
     misc_feature    4316..4836
                     /label=human beta actin promoter
     misc_feature    complement(4591..4610)
                     /label=bact-rev
     misc_feature    4591..4610
                     /label=bact-fwd
     misc_feature    4712..4731
                     /label=betact5
     CDS             4862..5656
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    4987..5006
                     /label=neoseq-fwd
     misc_feature    5031..5050
                     /label=neoPstI_5
     misc_feature    complement(5097..5116)
                     /label=neoseq-rev
     misc_feature    complement(5097..5116)
                     /label=neoseq-rev
     misc_feature    5368..5386
                     /label=neoSphI_5
     misc_feature    5526..5545
                     /label=neoseq2-fwd
     misc_feature    5661..5680
                     /label=neoBstB1_5
     primer_bind     complement(5825..5865)
                     /label=Fragment 2.REV
     polyA_signal    5851..5972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6031..6051)
                     /label=OJH_514 Seq_SV40polyA-Effector
     misc_feature    complement(6463..6772)
                     /label='Piggy 5'
     primer_bind     complement(7259..7275)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7283..7299
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7307..7337)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7352..7373
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7661..8249)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8255..8294)
                     /label=Hifi_catalitic_mut_all_R
     primer_bind     8275..8314
                     /label=Hifi_catalitic_mut_all_F
     CDS             complement(8420..9280)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     1081 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca
     1141 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     1201 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     1261 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     1321 acacctgggg ggatacgggg aaaaggcctc cacggccact agtctttcgt cttcaagaat
     1381 tcctcgagtt tactccctat cagtgataga gaacgtatga agagtttact ccctatcagt
     1441 gatagagaac gtatgcagac tttactccct atcagtgata gagaacgtat aaggagttta
     1501 ctccctatca gtgatagaga acgtatgacc agtttactcc ctatcagtga tagagaacgt
     1561 atctacagtt tactccctat cagtgataga gaacgtatat ccagtttact ccctatcagt
     1621 gatagagaac gtataagctt taggcgtgta cggtgggcgc ctataaaagc agagctcgtt
     1681 tagtgaaccg tcagatcgcc tggagcaatt ccacaacact tttgtcttat accaactttc
     1741 cgtaccactt cctaccctcg taaaggtcta gagctagcca tgggccccga catcgtgatg
     1801 acccagagcc ccagcagcct gagcgccagc gtgggcgacc gcgtgaccat cacctgccgc
     1861 agcagcaccg gcgccgtgac caccagcaac tacgccagct gggtgcagga gaagcccggc
     1921 aagctgttca agggcctgat cggcggcacc aacaaccgcg cccccggcgt gcccagccgc
     1981 ttcagcggca gcctgatcgg cgacaaggcc accctgacca tcagcagcct gcagcccgag
     2041 gacttcgcca cctacttctg cgccctgtgg tacagcaacc actgggtgtt cggccagggc
     2101 accaaggtgg agctgaagcg tggaggcgga ggtagtggag gtggaggctc tggtggggga
     2161 ggaagcagtg gaggcggaag tgaggtgaag ctgctggaga gcggaggcgg actggtgcag
     2221 cccggcggat ctctgaagct gagctgcgcc gtgagcggct tcagcctgac cgactacggc
     2281 gtgaactggg tgcgccaggc ccccggacgc ggactggagt ggatcggtgt gatctggggc
     2341 gacggcatca ccgactacaa cagcgccctg aaggaccgct tcatcatcag caaggacaac
     2401 ggcaagaaca ccgtgtacct gcagatgagc aaggtgcgca gcgacgacac cgccctgtac
     2461 tactgcgtga ccggcctgtt cgactactgg ggccagggca ccctggtgac cgtgagcagc
     2521 tacccatacg atgttccaga ttacgctggt ggaggcggag gttctggggg aggaggtagt
     2581 ggcggtggtg gttcaggagg cggcggaagc ttggatccag gtggaggtgg aagcggtagc
     2641 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     2701 aatgggcaca aattttctgt ccgtggagag ggtgaaggtg atgctacaaa cggaaaactc
     2761 acccttaaat ttatttgcac tactggaaaa ctacctgttc cgtggccaac acttgtcact
     2821 actctgacct atggtgttca atgcttttcc cgttatccgg atcacatgaa acggcatgac
     2881 tttttcaaga gtgccatgcc cgaaggttat gtacaggaac gcactatatc tttcaaagat
     2941 gacgggacct acaagacgcg tgctgaagtc aagtttgaag gtgataccct tgttaatcgt
     3001 atcgagttaa agggtattga ttttaaagaa gatggaaaca ttcttggaca caaactcgag
     3061 tacaacttta actcacacaa tgtatacatc acggcagaca aacaaaagaa tggaatcaaa
     3121 gctaacttca aaattcgcca caacgttgaa gatggttccg ttcaactagc agaccattat
     3181 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcg
     3241 acacaatctg tcctttcgaa agatcccaac gaaaagcgtg accacatggt ccttcttgag
     3301 tttgtaactg ctgctgggat tacacatggc atggatgagc tctacaaagg tggaggtcgg
     3361 accggcccca agaaaaaacg caaggtggaa gatcctaaga aaaagcggaa agtggacggc
     3421 attggtagtg ggagcaacgg cagcagcgga tccggaggcg gtggtacctc tcaggctgtg
     3481 cagacaaatg gaactcaacc attaagcaaa acatgggaac tcagtttgta tgagttacaa
     3541 cgaacacctc aggaggcaat aacagatggc ttggaaattg tggtttcacc tagaagtcta
     3601 cacagtgaat taatgtgccc aatttgtttg gatatgttaa agaacaccat gactacaaag
     3661 gagtgtttac atcggttttg cgcggattgt attatcacag cccttagaag tggcaacaaa
     3721 gagtgtccta cctgtcggaa aaaactggtt tctaaaagat cactaaggcc agacccgaac
     3781 tttgatgcac tcatcagcaa gatttatccc agtcgtgatg agtatgaagc gcatcaggaa
     3841 agggtcttag caaggatcaa caaacacaac aatcagcagg ctctcagcca cagcatcgag
     3901 gaggggctga agatacaggc catgaacaga ttacagcgag gcaaaaagca gcagatagaa
     3961 aatggtagtg gagcagaaga taatggtgac agctcccact gtagtaacgc atccacacac
     4021 agcaaccagg aagcgggccc gagtaacaaa cggaccaaaa cctcttgaca aaagatcata
     4081 atcagccata tcacatctgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     4141 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     4201 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     4261 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctc atatggcctt
     4321 ctgcaggagc gtacagaacc cagggccctg gcacccgtgc agaccctggc ccaccccacc
     4381 tgggcgctca gtgcccaaga gatgtccaca cctaggatgt cccgcggtgg gtggggggcc
     4441 cgagagacgg gcaggccggg ggcaggcctg gccatgcggg gccgaaccgg gcactgccca
     4501 gcgtgggcgc gggggccacg gcgcgcgccc ccagcccccg ggcccagcac cccaaggcgg
     4561 ccaacgccaa aactctccct cctcctcttc ctcaatctcg ctctcgctct tttttttttt
     4621 cgcaaaagga ggggagaggg ggtaaaaaaa tgctgcactg tgcggcgaag ccggtgagtg
     4681 agcggcgcgg ggccaatcag cgtgcgccgt tccgaaagtt gccttttatg gctcgagcgg
     4741 ccgcggcggc gccctataaa acccagcggc gcgacgcgcc accaccgccg agaccgcgtc
     4801 cgccccgcga gcacagagcc tcgcctttgc cgatcctcta gagtcgagat ccgccgccac
     4861 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4921 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4981 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     5041 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5101 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5161 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5221 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5281 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5341 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     5401 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5461 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5521 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5581 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5641 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     5701 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5761 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     5821 gcccaccccc cggatctaag ctctagagtt aacttgttta ttgcagctta taatggttac
     5881 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     5941 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggaattgact caaatgatgt
     6001 caattagtct atcagaagct atctggtctc ccttccgggg gacaagacat ccctgtttaa
     6061 tatttaaaca gcagtgttcc caaactgggt tcttatatcc cttgctctgg tcaaccaggt
     6121 tgcagggttt cctgtcctca caggaacgaa gtccctaaag aaacagtggc agccaggttt
     6181 agccccggaa ttgactggat tcctttttta gggcccattg gtatggcttt ttccccgtat
     6241 ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg
     6301 tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga
     6361 gcgccggacc ggagcggagc cccgggcggc tcgctgctgc cccctagcgg gggagggacg
     6421 taattacatc cctgggggct ttgggggggg gctgtccctg atatctataa caagaaaata
     6481 tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt atcgtatgag
     6541 ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac gcggtcgtta
     6601 tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga gctccaagcg
     6661 gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa gagagagcaa
     6721 tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt aatctagctg
     6781 catcaggatc atatcgtcgg gtcttttttc cggctcagtc atcgcccaag ctggcgctat
     6841 ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg aggttgaagc ggcatggaaa
     6901 gagtttgccg aggatgactg ctgctgcatt gacgttgagc gaaaacgcac gtttaccatg
     6961 atgattcggg aaggtgtggc catgcacgcc tttaacggtg aactgttcgt tcaggccacc
     7021 tgggatacca gttcgtcgcg gcttttccgg acacagttcc ggatggtcag cccgaagcgc
     7081 atcagcaacc cgaacaatac cggcgacagc cggaactgcc gtgccggtgt gcagattaat
     7141 gacagcggtg cggcgctggg atattacgtc agcgaggacg ggtatcctgg ctggatgccg
     7201 cagaaatgga catggatacc ccgtgagtta cccggcgggc gcgcttggcg taatcatggt
     7261 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7321 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7381 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7441 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7501 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7561 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7621 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7681 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7741 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7801 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7861 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7921 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7981 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8041 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8101 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8161 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8221 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8281 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8341 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8401 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8461 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8521 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8581 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8641 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8701 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8761 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8821 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8881 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8941 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9001 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9061 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9121 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9181 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9241 aaaagggaat aagggcgaca cggaaatgtt gaatactcat
//
