LOCUS       Exported               10084 bp ds-DNA     circular SYN 28-FEB-2025
DEFINITION  Dox-inducible expression of the engineered epigenetic effector Kmt5c
            fused with scFV-sfGFP to programme H4K20 methylation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Policarpi C, Munafo M, Tsagkris S, Carlini V, Hackett JA.
  TITLE     Systematic epigenome editing captures the context-dependent 
            instructive function of chromatin modifications.
  JOURNAL   Nat Genet 2024 Jun;56:1168-1180
  PUBMED    38724747
REFERENCE   2  (bases 1 to 10084)
  AUTHORS   Epitome Tx
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 28, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Under patent. Not for commercial usage or modification.
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(870..1104)
                     /label=Piggy 3'
     promoter        1386..1764
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    1394..1412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1430..1448
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1466..1484
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1502..1520
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1538..1556
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1574..1592
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1610..1628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1638..1658
                     /label=OJH_513 Seq_TRE3G-scFv
     primer_bind     1763..1798
                     /label=Fragment 1.FOR (2)
     CDS             1780..2481
                     /codon_start=1
                     /label=Recoded scFV
                     /translation="MGPDIVMTQSPSSLSASVGDRVTITCRSSTGAVTTSNYASWVQEK
                     PGKLFKGLIGGTNNRAPGVPSRFSGSLIGDKATLTISSLQPEDFATYFCALWYSNHWVF
                     GQGTKVELKRGGGGSGGGGSGGGGSSGGGSEVKLLESGGGLVQPGGSLKLSCAVSGFSL
                     TDYGVNWVRQAPGRGLEWIGVIWGDGITDYNSALKDRFIISKDNGKNTVYLQMSKVRSD
                     DTALYYCVTGLF"
     primer_bind     complement(2171..2190)
                     /label=scFV-recodedgenot_R
     CDS             2521..2547
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2548..2637
                     /codon_start=1
                     /label=Linker
                     /translation="GGGGGSGGGGSGGGGSGGGGSLDPGGGGSG"
     CDS             2638..3348
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     3193..3213
                     /label=OJH_511_Seq_sfGFP-effector
     CDS             3367..3387
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3394..3414
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3427..3462
                     /label=Linker
     primer_bind     complement(3447..3468)
                     /label=Fragment 1.REV (2)
     primer_bind     3449..3488
                     /label=p163A_KMT5C_cloning_F
     misc_feature    3469..4872
                     /label=Kmt5c FL
     primer_bind     complement(3891..3910)
                     /label=p163A_KMT5C_R1
     primer_bind     3994..4013
                     /label=p163A_KMT5C_seq_F1
     misc_feature    4009..4020
                     /label=NHDC to AAAG for catalytic mutant
     primer_bind     complement(4846..4887)
                     /label=p163A_KMT5C_cloning_R
     primer_bind     4873..4908
                     /label=Fragment 2.FOR
     misc_feature    4876..6651
     primer_bind     complement(4981..4998)
                     /label=Fragment 2.REV
     polyA_signal    4984..5105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    5116..6651
     misc_feature    5116..6651
     misc_feature    5120..5640
                     /label=human beta actin promoter
     misc_feature    5395..5414
                     /label=bact-fwd
     misc_feature    complement(5395..5414)
                     /label=bact-rev
     misc_feature    5516..5535
                     /label=betact5
     CDS             5666..6460
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    5791..5810
                     /label=neoseq-fwd
     misc_feature    5835..5854
                     /label=neoPstI_5
     misc_feature    complement(5901..5920)
                     /label=neoseq-rev
     misc_feature    complement(5901..5920)
                     /label=neoseq-rev
     misc_feature    6172..6190
                     /label=neoSphI_5
     misc_feature    6330..6349
                     /label=neoseq2-fwd
     misc_feature    6465..6484
                     /label=neoBstB1_5
     primer_bind     complement(6629..6669)
                     /label=Fragment 2.REV
     polyA_signal    6655..6776
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6835..6855)
                     /label=OJH_514 Seq_SV40polyA-Effector
     misc_feature    complement(7267..7576)
                     /label='Piggy 5'
     primer_bind     complement(8063..8079)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8087..8103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8111..8141)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8156..8177
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8465..9053)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9224..10084)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     1081 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca
     1141 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     1201 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     1261 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     1321 acacctgggg ggatacgggg aaaaggcctc cacggccact agtctttcgt cttcaagaat
     1381 tcctcgagtt tactccctat cagtgataga gaacgtatga agagtttact ccctatcagt
     1441 gatagagaac gtatgcagac tttactccct atcagtgata gagaacgtat aaggagttta
     1501 ctccctatca gtgatagaga acgtatgacc agtttactcc ctatcagtga tagagaacgt
     1561 atctacagtt tactccctat cagtgataga gaacgtatat ccagtttact ccctatcagt
     1621 gatagagaac gtataagctt taggcgtgta cggtgggcgc ctataaaagc agagctcgtt
     1681 tagtgaaccg tcagatcgcc tggagcaatt ccacaacact tttgtcttat accaactttc
     1741 cgtaccactt cctaccctcg taaaggtcta gagctagcca tgggccccga catcgtgatg
     1801 acccagagcc ccagcagcct gagcgccagc gtgggcgacc gcgtgaccat cacctgccgc
     1861 agcagcaccg gcgccgtgac caccagcaac tacgccagct gggtgcagga gaagcccggc
     1921 aagctgttca agggcctgat cggcggcacc aacaaccgcg cccccggcgt gcccagccgc
     1981 ttcagcggca gcctgatcgg cgacaaggcc accctgacca tcagcagcct gcagcccgag
     2041 gacttcgcca cctacttctg cgccctgtgg tacagcaacc actgggtgtt cggccagggc
     2101 accaaggtgg agctgaagcg tggaggcgga ggtagtggag gtggaggctc tggtggggga
     2161 ggaagcagtg gaggcggaag tgaggtgaag ctgctggaga gcggaggcgg actggtgcag
     2221 cccggcggat ctctgaagct gagctgcgcc gtgagcggct tcagcctgac cgactacggc
     2281 gtgaactggg tgcgccaggc ccccggacgc ggactggagt ggatcggtgt gatctggggc
     2341 gacggcatca ccgactacaa cagcgccctg aaggaccgct tcatcatcag caaggacaac
     2401 ggcaagaaca ccgtgtacct gcagatgagc aaggtgcgca gcgacgacac cgccctgtac
     2461 tactgcgtga ccggcctgtt cgactactgg ggccagggca ccctggtgac cgtgagcagc
     2521 tacccatacg atgttccaga ttacgctggt ggaggcggag gttctggggg aggaggtagt
     2581 ggcggtggtg gttcaggagg cggcggaagc ttggatccag gtggaggtgg aagcggtagc
     2641 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     2701 aatgggcaca aattttctgt ccgtggagag ggtgaaggtg atgctacaaa cggaaaactc
     2761 acccttaaat ttatttgcac tactggaaaa ctacctgttc cgtggccaac acttgtcact
     2821 actctgacct atggtgttca atgcttttcc cgttatccgg atcacatgaa acggcatgac
     2881 tttttcaaga gtgccatgcc cgaaggttat gtacaggaac gcactatatc tttcaaagat
     2941 gacgggacct acaagacgcg tgctgaagtc aagtttgaag gtgataccct tgttaatcgt
     3001 atcgagttaa agggtattga ttttaaagaa gatggaaaca ttcttggaca caaactcgag
     3061 tacaacttta actcacacaa tgtatacatc acggcagaca aacaaaagaa tggaatcaaa
     3121 gctaacttca aaattcgcca caacgttgaa gatggttccg ttcaactagc agaccattat
     3181 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcg
     3241 acacaatctg tcctttcgaa agatcccaac gaaaagcgtg accacatggt ccttcttgag
     3301 tttgtaactg ctgctgggat tacacatggc atggatgagc tctacaaagg tggaggtcgg
     3361 accggcccca agaaaaaacg caaggtggaa gatcctaaga aaaagcggaa agtggacggc
     3421 attggtagtg ggagcaacgg cagcagcgga tccggaggcg gtggtaccgg gcctgatcga
     3481 gtgacagccc gagaactgtg cgagaatgat gacctggcca ccagccttgt tcttgacccc
     3541 tacctcggct tccgcaccca taagatgaat gtcagtcctg tgcccacctt gcgacgacaa
     3601 caccaccttc gttcagcact ggaagctttc ctaaggcaac gggacctgga ggctgcattc
     3661 cgggccctga cgctaggagg ctggatggcc cactacttcc agagccgtgc ccctcggcag
     3721 gaggctgctc tcaagaccca cattttctgc tacctccgcg ccttcctgcc tgagagtggc
     3781 ttcaccattc tgccctgcac ccgctactcc atggagacca atggggccaa gattgtgtct
     3841 acccgtgctt ggaagaagaa tgagaagctg gagctgctgg tgggctgcat tgcagagctg
     3901 cgtgaagagg atgaagacct gcttagggcc ggtgagaacg acttcagcat catgtactcc
     3961 acccgaaaaa ggagtgccca gctgtggctt ggcccagctg ccttcatcaa ccatgactgc
     4021 aaacccaact gcaagtttgt gccctcagat gggaatactg cctgtgtgaa ggtgctacgg
     4081 gacattgagc caggggatga agtgacttgc ttctatggtg agggcttctt cggcgagaag
     4141 aatgagcact gtgaatgcta tacctgtgag aggaaaggcg agggagcttt cagacttcag
     4201 cccagggagc ctgagctgcg accaaagccc ctagacaagt atgagctccg agagacaaag
     4261 aggcgtctgc agcagggctt ggtcagcagc cagcagagcc tgatgagccg gtgggcctgc
     4321 tcccacctgt ccccactgcg ccctgaccca ttctgtgctg cctgccagcc ctcatgcctg
     4381 ctgcctgcca gcccccacat ggattacttg cccctttggc tccagcgggc gcctcaaccc
     4441 cagcccatag tacctcccag gaagcgccac cgccgccgcc gccccaggat ccgccaggct
     4501 tcattgcctc ctgtcctccg cactgcctgt gtccccctgc accgatgggg aggctgtggt
     4561 ccccactgcc agctacgtgc tgaggccatg gtgaccctac acctgcgccc ccagactcgc
     4621 tggaccccac aacaggactg gtactgggcc cggcgctatg ggctaccttc tgtggggcgt
     4681 gtggaactta cccgcctagc cccagccctg ccagctgctc ctgcccctgc tgggaaccca
     4741 ggtcctgtcc ccactcctga tttcatccct aagcaggccc ttgcctttgc tccgttctgc
     4801 ccacctaagc gcctccggct agtggtcagt catggttcta ttgatctgga catcaatagt
     4861 ggtgagccat gacaaaagat cataatcagc catatcacat ctgtagaggt tttacttgct
     4921 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     4981 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     5041 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     5101 tcttatcatg tctcatatgg ccttctgcag gagcgtacag aacccagggc cctggcaccc
     5161 gtgcagaccc tggcccaccc cacctgggcg ctcagtgccc aagagatgtc cacacctagg
     5221 atgtcccgcg gtgggtgggg ggcccgagag acgggcaggc cgggggcagg cctggccatg
     5281 cggggccgaa ccgggcactg cccagcgtgg gcgcgggggc cacggcgcgc gcccccagcc
     5341 cccgggccca gcaccccaag gcggccaacg ccaaaactct ccctcctcct cttcctcaat
     5401 ctcgctctcg ctcttttttt ttttcgcaaa aggaggggag agggggtaaa aaaatgctgc
     5461 actgtgcggc gaagccggtg agtgagcggc gcggggccaa tcagcgtgcg ccgttccgaa
     5521 agttgccttt tatggctcga gcggccgcgg cggcgcccta taaaacccag cggcgcgacg
     5581 cgccaccacc gccgagaccg cgtccgcccc gcgagcacag agcctcgcct ttgccgatcc
     5641 tctagagtcg agatccgccg ccaccatgat tgaacaagat ggattgcacg caggttctcc
     5701 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     5761 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     5821 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     5881 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     5941 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     6001 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     6061 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     6121 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     6181 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     6241 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     6301 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     6361 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     6421 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     6481 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     6541 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     6601 ggggatctca tgctggagtt cttcgcccac cccccggatc taagctctag agttaacttg
     6661 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6721 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6781 gtctggaatt gactcaaatg atgtcaatta gtctatcaga agctatctgg tctcccttcc
     6841 gggggacaag acatccctgt ttaatattta aacagcagtg ttcccaaact gggttcttat
     6901 atcccttgct ctggtcaacc aggttgcagg gtttcctgtc ctcacaggaa cgaagtccct
     6961 aaagaaacag tggcagccag gtttagcccc ggaattgact ggattccttt tttagggccc
     7021 attggtatgg ctttttcccc gtatcccccc aggtgtctgc aggctcaaag agcagcgaga
     7081 agcgttcaga ggaaagcgat cccgtgccac cttccccgtg cccgggctgt ccccgcacgc
     7141 tgccggctcg gggatgcggg gggagcgccg gaccggagcg gagccccggg cggctcgctg
     7201 ctgcccccta gcgggggagg gacgtaatta catccctggg ggctttgggg gggggctgtc
     7261 cctgatatct ataacaagaa aatatatata taataagtta tcacgtaagt agaacatgaa
     7321 ataacaatat aattatcgta tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg
     7381 tcattttgac tcacgcggtc gttatagttc aaaatcagtg acacttaccg cattgacaag
     7441 cacgcctcac gggagctcca agcggcgact gagatgtcct aaatgcacag cgacggattc
     7501 gcgctattta gaaagagaga gcaatatttc aagaatgcat gcgtcaattt tacgcagact
     7561 atctttctag ggttaatcta gctgcatcag gatcatatcg tcgggtcttt tttccggctc
     7621 agtcatcgcc caagctggcg ctatctgggc atcggggagg aagaagcccg tgccttttcc
     7681 cgcgaggttg aagcggcatg gaaagagttt gccgaggatg actgctgctg cattgacgtt
     7741 gagcgaaaac gcacgtttac catgatgatt cgggaaggtg tggccatgca cgcctttaac
     7801 ggtgaactgt tcgttcaggc cacctgggat accagttcgt cgcggctttt ccggacacag
     7861 ttccggatgg tcagcccgaa gcgcatcagc aacccgaaca ataccggcga cagccggaac
     7921 tgccgtgccg gtgtgcagat taatgacagc ggtgcggcgc tgggatatta cgtcagcgag
     7981 gacgggtatc ctggctggat gccgcagaaa tggacatgga taccccgtga gttacccggc
     8041 gggcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     8101 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     8161 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     8221 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     8281 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     8341 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     8401 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8461 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8521 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8581 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8641 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8701 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8761 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8821 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8881 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     8941 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     9001 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     9061 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     9121 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9181 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     9241 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9301 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9361 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9421 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9481 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9541 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9601 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9661 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9721 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9781 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9841 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9901 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9961 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    10021 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    10081 tcat
//
