LOCUS       Exported               10000 bp ds-DNA     circular SYN 27-FEB-2025
DEFINITION  Dox-inducible expression of the engineered catalytic-domain (CD) of 
            epigenetic effector Dot1l fused with scFV-sfGFP to programme H3K79 
            methylation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   Policarpi C, Munafo M, Tsagkris S, Carlini V, Hackett JA.
  TITLE     Systematic epigenome editing captures the context-dependent 
            instructive function of chromatin modifications.
  JOURNAL   Nat Genet 2024 Jun;56:1168-1180
  PUBMED    38724747
REFERENCE   2  (bases 1 to 10000)
  AUTHORS   Epitome Tx
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 28, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Under patent. Not for commercial usage or modification.
FEATURES             Location/Qualifiers
     source          1..10000
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(870..1104)
                     /label=Piggy 3'
     promoter        1386..1764
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    1394..1412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1430..1448
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1466..1484
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1502..1520
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1538..1556
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1574..1592
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1610..1628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1711..1730
                     /label=Primer 1
     primer_bind     1763..1798
                     /label=p163_Frag1_F
     CDS             1780..2481
                     /codon_start=1
                     /label=Recoded scFV
                     /translation="MGPDIVMTQSPSSLSASVGDRVTITCRSSTGAVTTSNYASWVQEK
                     PGKLFKGLIGGTNNRAPGVPSRFSGSLIGDKATLTISSLQPEDFATYFCALWYSNHWVF
                     GQGTKVELKRGGGGSGGGGSGGGGSSGGGSEVKLLESGGGLVQPGGSLKLSCAVSGFSL
                     TDYGVNWVRQAPGRGLEWIGVIWGDGITDYNSALKDRFIISKDNGKNTVYLQMSKVRSD
                     DTALYYCVTGLF"
     primer_bind     complement(2171..2190)
                     /label=scFV-recodedgenot_R
     primer_bind     2341..2360
                     /label=Primer 2
     CDS             2521..2547
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2548..2637
                     /codon_start=1
                     /label=Linker
                     /translation="GGGGGSGGGGSGGGGSGGGGSLDPGGGGSG"
     CDS             2638..3348
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     2971..2990
                     /label=Primer 3
     primer_bind     3193..3213
                     /label=OJH_511
     CDS             3367..3387
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3394..3414
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3427..3462
                     /label=Linker
     primer_bind     complement(3445..3465)
                     /label=p163_Frag1_R
     primer_bind     3449..3519
                     /label=Fragment.FOR
     CDS             3466..3492
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     misc_feature    3496..4779
                     /label=Dot1L CD
     misc_feature    4213..4215
                     /label=N to A catalytic mutant
     primer_bind     complement(4755..4803)
                     /label=NEW_DOT1L_cloning_R
     primer_bind     4789..4822
                     /label=p163_Frag2_F
     misc_feature    4792..6567
     primer_bind     4832..4851
                     /label=Primer 4
     primer_bind     complement(4897..4914)
                     /label=p163_Frag2_R
     polyA_signal    4900..5021
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    5032..6567
     misc_feature    5032..6567
     misc_feature    5036..5556
                     /label=human beta actin promoter
     misc_feature    5311..5330
                     /label=bact-fwd
     misc_feature    complement(5311..5330)
                     /label=bact-rev
     misc_feature    5432..5451
                     /label=betact5
     primer_bind     5462..5481
                     /label=Primer 5
     CDS             5582..6376
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    5707..5726
                     /label=neoseq-fwd
     misc_feature    5751..5770
                     /label=neoPstI_5
     misc_feature    complement(5817..5836)
                     /label=neoseq-rev
     misc_feature    complement(5817..5836)
                     /label=neoseq-rev
     misc_feature    6088..6106
                     /label=neoSphI_5
     primer_bind     6092..6111
                     /label=Primer 6
     misc_feature    6246..6265
                     /label=neoseq2-fwd
     misc_feature    6381..6400
                     /label=neoBstB1_5
     primer_bind     complement(6545..6585)
                     /label=p163_Frag2_R
     polyA_signal    6571..6692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6751..6771)
                     /label=OJH_514
     misc_feature    complement(7183..7492)
                     /label='Piggy 5'
     primer_bind     complement(7979..7995)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8003..8019
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8027..8057)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8072..8093
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8381..8969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9140..10000)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     1081 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca
     1141 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     1201 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     1261 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     1321 acacctgggg ggatacgggg aaaaggcctc cacggccact agtctttcgt cttcaagaat
     1381 tcctcgagtt tactccctat cagtgataga gaacgtatga agagtttact ccctatcagt
     1441 gatagagaac gtatgcagac tttactccct atcagtgata gagaacgtat aaggagttta
     1501 ctccctatca gtgatagaga acgtatgacc agtttactcc ctatcagtga tagagaacgt
     1561 atctacagtt tactccctat cagtgataga gaacgtatat ccagtttact ccctatcagt
     1621 gatagagaac gtataagctt taggcgtgta cggtgggcgc ctataaaagc agagctcgtt
     1681 tagtgaaccg tcagatcgcc tggagcaatt ccacaacact tttgtcttat accaactttc
     1741 cgtaccactt cctaccctcg taaaggtcta gagctagcca tgggccccga catcgtgatg
     1801 acccagagcc ccagcagcct gagcgccagc gtgggcgacc gcgtgaccat cacctgccgc
     1861 agcagcaccg gcgccgtgac caccagcaac tacgccagct gggtgcagga gaagcccggc
     1921 aagctgttca agggcctgat cggcggcacc aacaaccgcg cccccggcgt gcccagccgc
     1981 ttcagcggca gcctgatcgg cgacaaggcc accctgacca tcagcagcct gcagcccgag
     2041 gacttcgcca cctacttctg cgccctgtgg tacagcaacc actgggtgtt cggccagggc
     2101 accaaggtgg agctgaagcg tggaggcgga ggtagtggag gtggaggctc tggtggggga
     2161 ggaagcagtg gaggcggaag tgaggtgaag ctgctggaga gcggaggcgg actggtgcag
     2221 cccggcggat ctctgaagct gagctgcgcc gtgagcggct tcagcctgac cgactacggc
     2281 gtgaactggg tgcgccaggc ccccggacgc ggactggagt ggatcggtgt gatctggggc
     2341 gacggcatca ccgactacaa cagcgccctg aaggaccgct tcatcatcag caaggacaac
     2401 ggcaagaaca ccgtgtacct gcagatgagc aaggtgcgca gcgacgacac cgccctgtac
     2461 tactgcgtga ccggcctgtt cgactactgg ggccagggca ccctggtgac cgtgagcagc
     2521 tacccatacg atgttccaga ttacgctggt ggaggcggag gttctggggg aggaggtagt
     2581 ggcggtggtg gttcaggagg cggcggaagc ttggatccag gtggaggtgg aagcggtagc
     2641 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     2701 aatgggcaca aattttctgt ccgtggagag ggtgaaggtg atgctacaaa cggaaaactc
     2761 acccttaaat ttatttgcac tactggaaaa ctacctgttc cgtggccaac acttgtcact
     2821 actctgacct atggtgttca atgcttttcc cgttatccgg atcacatgaa acggcatgac
     2881 tttttcaaga gtgccatgcc cgaaggttat gtacaggaac gcactatatc tttcaaagat
     2941 gacgggacct acaagacgcg tgctgaagtc aagtttgaag gtgataccct tgttaatcgt
     3001 atcgagttaa agggtattga ttttaaagaa gatggaaaca ttcttggaca caaactcgag
     3061 tacaacttta actcacacaa tgtatacatc acggcagaca aacaaaagaa tggaatcaaa
     3121 gctaacttca aaattcgcca caacgttgaa gatggttccg ttcaactagc agaccattat
     3181 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcg
     3241 acacaatctg tcctttcgaa agatcccaac gaaaagcgtg accacatggt ccttcttgag
     3301 tttgtaactg ctgctgggat tacacatggc atggatgagc tctacaaagg tggaggtcgg
     3361 accggcccca agaaaaaacg caaggtggaa gatcctaaga aaaagcggaa agtggacggc
     3421 attggtagtg ggagcaacgg cagcagcgga tccggaggcg gtggtcctgc tgcaaagcgt
     3481 gttaagcttg atggcggcga gaagctggag ctgaggctca agtcgcctgt gggcgccgag
     3541 cccgccgtct acccgtggcc gctgccggtc tacgacaagc accatgatgc tgctcatgag
     3601 attattgaga ctatccggtg ggtctgcgag gaaatcccag atctcaagct ggccatggag
     3661 aactatgtcc tgatcgacta cgacaccaaa agttttgaaa gcatgcagcg gctgtgtgac
     3721 aaatacaacc gggccatcga cagcatccac cagctgtgga agggcactac acagcccatg
     3781 aagctgaata ctcggccgtc caatggactt ctgcggcaca tcctgcagca ggtatataac
     3841 cactctgtca ctgacccgga gaagcttaac aactacgagc ccttctcccc tgaggtgtat
     3901 ggggagacct cctttgacct ggtggcccag atgattgatg agatcaagat gacagaggat
     3961 gacctgtttg tcgacctggg cagtggtgtg gggcaggttg tccttcaggt tgctgcggcc
     4021 accaactgca aacatcacta cggagtggag aaagcggaca tcccagccaa gtacgcagag
     4081 accatggacc gagagttcag gaagtggatg aaatggtatg gaaaaaagca tgcagaatac
     4141 acactggaac gaggtgactt cctctcagag gaatggaggg aacggatcgc caacacgagt
     4201 gttatatttg tgaataactt tgcctttggt cctgaggtgg atcaccagct gaaggagcga
     4261 ttcgcaaaca tgaaggaagg cggcagaatc gtatcctcaa agccctttgc ccctctgaac
     4321 ttcaggatca acagcaggaa cttgagtgac attggcacca tcatgcgtgt ggtggagctg
     4381 tcgcccctga agggctctgt gtcgtggact ggcaagcctg tctcctacta tctgcacacc
     4441 attgaccgca ccatacttga aaactatttt tctagtctga aaaatccaaa actcagggag
     4501 gagcaggagg cagctaggcg aagacagcag cgagagaaca agagcaatgc gaccacgccc
     4561 accaaagtcc ctgagagcaa agcagctgcc actgaggccc ccgcggattc tggtgctgag
     4621 gaagaaaaat caggtgtggc tactgtcaaa aagccatctc cctccaaagc ccggaagaag
     4681 aaactgaaca agaaagggag aaagatggct ggccggaaac gtgggcggcc caagaaaatg
     4741 agtgctgcca gtgccgaacg caagtctaag aagagccaaa gatcttgaca aaagatcata
     4801 atcagccata tcacatctgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     4861 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     4921 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     4981 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctc atatggcctt
     5041 ctgcaggagc gtacagaacc cagggccctg gcacccgtgc agaccctggc ccaccccacc
     5101 tgggcgctca gtgcccaaga gatgtccaca cctaggatgt cccgcggtgg gtggggggcc
     5161 cgagagacgg gcaggccggg ggcaggcctg gccatgcggg gccgaaccgg gcactgccca
     5221 gcgtgggcgc gggggccacg gcgcgcgccc ccagcccccg ggcccagcac cccaaggcgg
     5281 ccaacgccaa aactctccct cctcctcttc ctcaatctcg ctctcgctct tttttttttt
     5341 cgcaaaagga ggggagaggg ggtaaaaaaa tgctgcactg tgcggcgaag ccggtgagtg
     5401 agcggcgcgg ggccaatcag cgtgcgccgt tccgaaagtt gccttttatg gctcgagcgg
     5461 ccgcggcggc gccctataaa acccagcggc gcgacgcgcc accaccgccg agaccgcgtc
     5521 cgccccgcga gcacagagcc tcgcctttgc cgatcctcta gagtcgagat ccgccgccac
     5581 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     5641 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     5701 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     5761 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5821 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5881 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5941 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     6001 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     6061 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     6121 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     6181 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     6241 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     6301 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     6361 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     6421 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     6481 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     6541 gcccaccccc cggatctaag ctctagagtt aacttgttta ttgcagctta taatggttac
     6601 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6661 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggaattgact caaatgatgt
     6721 caattagtct atcagaagct atctggtctc ccttccgggg gacaagacat ccctgtttaa
     6781 tatttaaaca gcagtgttcc caaactgggt tcttatatcc cttgctctgg tcaaccaggt
     6841 tgcagggttt cctgtcctca caggaacgaa gtccctaaag aaacagtggc agccaggttt
     6901 agccccggaa ttgactggat tcctttttta gggcccattg gtatggcttt ttccccgtat
     6961 ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg
     7021 tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga
     7081 gcgccggacc ggagcggagc cccgggcggc tcgctgctgc cccctagcgg gggagggacg
     7141 taattacatc cctgggggct ttgggggggg gctgtccctg atatctataa caagaaaata
     7201 tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt atcgtatgag
     7261 ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac gcggtcgtta
     7321 tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga gctccaagcg
     7381 gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa gagagagcaa
     7441 tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt aatctagctg
     7501 catcaggatc atatcgtcgg gtcttttttc cggctcagtc atcgcccaag ctggcgctat
     7561 ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg aggttgaagc ggcatggaaa
     7621 gagtttgccg aggatgactg ctgctgcatt gacgttgagc gaaaacgcac gtttaccatg
     7681 atgattcggg aaggtgtggc catgcacgcc tttaacggtg aactgttcgt tcaggccacc
     7741 tgggatacca gttcgtcgcg gcttttccgg acacagttcc ggatggtcag cccgaagcgc
     7801 atcagcaacc cgaacaatac cggcgacagc cggaactgcc gtgccggtgt gcagattaat
     7861 gacagcggtg cggcgctggg atattacgtc agcgaggacg ggtatcctgg ctggatgccg
     7921 cagaaatgga catggatacc ccgtgagtta cccggcgggc gcgcttggcg taatcatggt
     7981 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8041 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8101 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8161 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8221 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8281 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8341 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8401 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8461 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8521 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8581 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8641 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8701 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8761 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8821 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8881 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8941 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9001 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9061 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9121 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9181 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9241 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9301 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9361 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9421 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9481 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9541 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9601 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9661 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9721 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9781 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9841 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9901 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9961 aaaagggaat aagggcgaca cggaaatgtt gaatactcat
//
