LOCUS       P750-1                  8397 bp    DNA     circular SYN 16-AUG-2024
DEFINITION  P750-1.
ACCESSION   P750-1
VERSION     P750-1
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     promoter        complement(5728..6906)
                     /note="pLannotate"
                     /label="EF-1α promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(1564..5665)
                     /note="pLannotate"
                     /label="dCas9 (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.9"
                     /fragment="True"
                     /other="CDS"
     promoter        7262..7366
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             7367..8227
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(5665..5712)
                     /note="pLannotate"
                     /label="nucleoplasmin NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(1062..1286)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     protein_bind    877..898
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    951..967
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     misc_feature    7147..7191
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="31.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(6913..6979)
                     /note="pLannotate"
                     /label="small t antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="12.8"
                     /fragment="True"
                     /other="CDS"
     CDS             772..864
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(1016..1032)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="15.7"
                     /fragment="True"
                     /other="misc_feature"
     promoter        complement(1037..1058)
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
     CDS             complement(1328..1344)
                     /note="pLannotate"
                     /label="VP96 (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="7.5"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccctag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtatc gagccccagc tggttctttc cgcctcagaa gccatagagc ccaccgcatc
     1081 cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc
     1141 ccccagaata gaatgacacc tactcagaca atgcgatgca attttctcat tttattagga
     1201 aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac
     1261 agatggctgg caactagaag gcacagtcga ggctgatcag cgggtttaaa ctcacagcat
     1321 gtccaggtcg aaatcatcaa gggcgtcact gccgagcatg tcgaggtcaa agtcatcaag
     1381 ggcatccgaa ccaagcatgt caaggtcaaa atcatcgagg gcgtcacttc ccagcatatc
     1441 cagatcaaaa tcgtccaatg cgtcagcccg tccggatcca gcggccgcca ccttcctctt
     1501 tttcttaggt ccgctacctc ctcctccgct tcctcctcct ccgcttccac ctcctccagc
     1561 gctgtcgcct cccagctgag acaggtcgat ccgtgtctcg tacaggccgg tgatgctctg
     1621 gtggatcagg gtggcgtcca gcacctcttt ggtgctggtg tacctcttcc ggtcgatggt
     1681 ggtgtcaaag tacttgaagg cggcaggggc tcccagattg gtcagggtaa acaggtggat
     1741 gatattctcg gcctgctctc tgatgggctt atcccggtgc ttgttgtagg cggacagcac
     1801 tttgtccaga ttagcgtcgg ccaggatcac tctcttggag aactcgctga tctgctcgat
     1861 gatctcgtcc aggtagtgct tgtgctgttc cacaaacagc tgtttctgct cattatcctc
     1921 gggggagccc ttcagcttct catagtggct ggccaggtac aggaagttca catatttgga
     1981 gggcagggcc agttcgtttc ccttctgcag ttcgccggca gaggccagca ttctcttccg
     2041 gccgttttcc agctcgaaca gggagtactt aggcagcttg atgatcaggt cctttttcac
     2101 ttctttgtag cccttggctt ccagaaagtc gatgggattc ttctcgaagc tgcttctttc
     2161 catgatggtg atccccagca gctctttcac actcttcagt ttcttggact tgcccttttc
     2221 cactttggcc accaccagca cagaataggc cacggtgggg ctgtcgaagc cgccgtactt
     2281 cttagggtcc cagtccttct ttctggcgat cagcttatcg ctgttcctct tgggcaggat
     2341 agactctttg ctgaagccgc ctgtctgcac ctcggtcttt ttcacgatat tcacttgggg
     2401 catgctcagc actttccgca cggtggcaaa atcccggccc ttatcccaca cgatctcccc
     2461 ggtttcgccg tttgtctcga tcagaggccg cttccggatc tcgccgttgg ccagggtaat
     2521 ctcggtcttg aaaaagttca tgatgttgct gtagaagaag tacttggcgg tagccttgcc
     2581 gatttcctgc tcgctcttgg cgatcatctt ccgcacgtcg tacaccttgt agtcgccgta
     2641 cacgaactcg ctttccagct tagggtactt tttgatcagg gcggttccca cgacggcgtt
     2701 caggtaggcg tcgtgggcgt ggtggtagtt gttgatctcg cgcactttgt aaaactggaa
     2761 atccttccgg aaatcggaca ccagcttgga cttcagggtg atcactttca cttcccggat
     2821 cagcttgtca ttctcgtcgt acttagtgtt catccgggag tccaggatct gtgccacgtg
     2881 ctttgtgatc tgccgggttt ccaccagctg tctcttgatg aagccggcct tatccagttc
     2941 gctcaggccg cctctctcgg ccttggtcag attgtcgaac tttctctggg taatcagctt
     3001 ggcgttcagc agctgccgcc agtagttctt catcttcttc acgacctctt cggagggcac
     3061 gttgtcgctc ttgccccggg ccttgtcgct tctggtcagc accttgttgt cgatggagtc
     3121 gtccttcaga aagctctgag gcacgatgtg gtccacatcg tagtcggaca gccggttgat
     3181 gtccagttcc tggtccacgt acatatcccg cccattctgc aggtagtaca ggtacagctt
     3241 ctcgttctgc agctgggtgt tttccacggg gtgttctttc aggatctggc tgcccagctc
     3301 tttgatgccc tcttcgatcc gcttcattct ctcgcggctg ttcttctgtc ccttctgggt
     3361 ggtctggttc tctctggcca tttcgatcac gatgttctcg ggcttgtgcc ggcccatcac
     3421 tttcacgagc tcgtccacca ccttcactgt ctgcaggatg cccttcttaa tggcggggct
     3481 gccggccaga ttggcaatgt gctcgtgcag gctatcgccc tggccggaca cctgggcttt
     3541 ctggatgtcc tctttaaagg tcaggctgtc gtcgtggatc agctgcatga agtttctgtt
     3601 ggcgaagccg tcggacttca ggaaatccag gattgtcttg ccggactgct tgtcccggat
     3661 gccgttgatc agcttccggc tcagcctgcc ccagccggtg tatctccgcc gcttcagctg
     3721 cttcatcact ttgtcgtcga acaggtgggc ataggttttc agccgttcct cgatcatctc
     3781 tctgtcctca aacagtgtca gggtcagcac gatatcttcc agaatgtcct cgttttcctc
     3841 attgtccagg aagtccttgt ccttgataat tttcagcaga tcgtggtatg tgcccaggga
     3901 ggcgttgaac cgatcttcca cgccggagat ttccacggag tcgaagcact cgattttctt
     3961 gaagtagtcc tctttcagct gcttcacggt cactttccgg ttggtcttga acagcaggtc
     4021 cacgatggcc tttttctgct cgccgctcag gaaggcgggc tttctcattc cctcggtcac
     4081 gtatttcact ttggtcagct cgttatacac ggtgaagtac tcgtacagca ggctgtgctt
     4141 gggcagcacc ttctcgttgg gcaggttctt atcgaagttg gtcatccgct cgatgaagct
     4201 ctgggcggaa gcgcccttgt ccaccacttc ctcgaagttc cagggggtga tggtttcctc
     4261 gctctttctg gtcatccagg cgaatctgct gtttcccctg gccagagggc ccacgtagta
     4321 ggggatgcgg aaggtcagga tcttctcgat cttttcccgg ttgtccttca ggaatgggta
     4381 aaaatcttcc tgccgccgca gaatggcgtg cagctctccc aggtggatct ggtgggggat
     4441 gctgccgttg tcgaaggtcc gctgcttccg cagcaggtcc tctctgttca gcttcacgag
     4501 cagttcctcg gtgccgtcca tcttttccag gatgggcttg atgaacttgt agaactcttc
     4561 ctggctggct ccgccgtcaa tgtagccggc gtagccgttc ttgctctggt cgaagaaaat
     4621 ctctttgtac ttctcaggca gctgctgccg cacgagagct ttcagcaggg tcaggtcctg
     4681 gtggtgctcg tcgtatctct tgatcataga ggcgctcagg ggggccttgg tgatctcggt
     4741 gttcactctc aggatgtcgc tcagcaggat ggcgtcggac aggttcttgg cggccagaaa
     4801 caggtcggcg tactggtcgc cgatctgggc cagcaggttg tccaggtcgt cgtcgtaggt
     4861 gtccttgctc agctgcagtt tggcatcctc ggccaggtcg aagttgctct tgaagttggg
     4921 ggtcaggccc aggctcaggg caatcaggtt gccgaacagg ccattcttct tctcgccggg
     4981 cagctgggcg atcagatttt ccagccgtct gctcttgctc agtctggcag acaggatggc
     5041 cttggcgtcc acgccgctgg cgttgatggg gttttcctcg aacagctggt tgtaggtctg
     5101 caccagctgg atgaacagct tgtccacgtc gctgttgtcg gggttcaggt cgccctcgat
     5161 caggaagtgg ccccggaact tgatcatgtg ggccagggcc agatagatca gccgcaggtc
     5221 ggccttgtcg gtgctgtcca ccagtttctt tctcaggtgg tagatggtgg ggtacttctc
     5281 gtggtaggcc acctcgtcca cgatgttgcc gaagatgggg tgccgctcgt gcttcttatc
     5341 ctcttccacc aggaaggact cttccagtct gtggaagaag ctgtcgtcca ccttggccat
     5401 ctcgttgctg aagatctctt gcagatagca gatccggttc ttccgtctgg tgtatcttct
     5461 tctggcggtt ctcttcagcc gggtggcctc ggctgtttcg ccgctgtcga acagcagggc
     5521 tccgatcagg ttcttcttga tgctgtgccg gtcggtgttg cccagcacct tgaatttctt
     5581 gctgggcacc ttgtactcgt cggtgatcac ggcccagccc acagagttgg tgccgatggc
     5641 caggccgatg ctgtacttct tgtccttttt cttttttgcc tggccggcct ttttcgtggc
     5701 cgccggcctt ttcatggtgg ccgtacgtca cgacacctga aatggaagaa aaaaactttg
     5761 aaccactgtc tgaggcttga gaatgaacca agatccaaac tcaaaaaggg caaattccaa
     5821 ggagaattac atcaagtgcc aagctggcct aacttcagtc tccacccact cagtgtgggg
     5881 aaactccatc gcataaaacc cctcccccca acctaaagac gacgtactcc aaaagctcga
     5941 gaactaatcg aggtgcctgg acggcgcccg gtactccgtg gagtcacatg aagcgacggc
     6001 tgaggacgga aaggcccttt tcctttgtgt gggtgactca cccgcccgct ctcccgagcg
     6061 ccgcgtcctc cattttgagc tccctgcagc agggccggga agcggccatc tttccgctca
     6121 cgcaactggt gccgaccggg ccagccttgc cgcccagggc ggggcgatac acggcggcgc
     6181 gaggccaggc accagagcag gccggccagc ttgagactac ccccgtccga ttctcggtgg
     6241 ccgcgctcgc aggccccgcc tcgccgaaca tgtgcgctgg gacgcacggg ccccgtcgcc
     6301 gcccgcggcc ccaaaaaccg aaataccagt gtgcagatct tggcccgcat ttacaagact
     6361 atcttgccag aaaaaaagcg tcgcagcagg tcatcaaaaa ttttaaatgg ctagagactt
     6421 atcgaaagca gcgagacagg cgcgaaggtg ccaccagatt cgcacgcggc ggccccagcg
     6481 cccaggccag gcctcaactc aagcacgagg cgaaggggct ccttaagcgc aaggcctcga
     6541 actctcccac ccacttccaa cccgaagctc gggatcaaga atcacgtact gcagccaggt
     6601 ggaagtaatt caaggcacgc aagggccata acccgtaaag aggccaggcc cgcgggaacc
     6661 acacacggca cttacctgtg ttctggcggc aaacccgttg cgaaaaagaa cgttcacggc
     6721 gactactgca cttatatacg gttctccccc accctcggga aaaaggcgga gccagtacac
     6781 gacatcactt tcccagttta ccccgcgcca ccttctctag gcaccggttc aattgccgac
     6841 ccctcccccc aacttctcgg ggactgtggg cgatgtgcgc tctgcccact gacgggcacc
     6901 ggagccaatt cccactcctt tcaagaccta gaaggtccat tagctgcaaa gattcctctc
     6961 tgtttaaaac tttatccatc tttgcagcta gcaacgcgta tatctggccc gtacatcgcg
     7021 aagcagcgca aaacgcctaa ccctaagcag attcttcatg caattgtcgg tcaagccttg
     7081 ccttgttgta gcttaaattt tgctcgcgca ctactcagcg acctccaaca cacaagcagg
     7141 gagcagatac tggcttaact atgcggcatc agagcagatt gtactgagag tcgaccctag
     7201 gggatcggga gatcgatctc ccgatccgtc gacgtcaggt ggcacttttc ggggaaatgt
     7261 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7321 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7381 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7441 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7501 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7561 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7621 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7681 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7741 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7801 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7861 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7921 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7981 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8041 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8101 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8161 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8221 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8281 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8341 acgtgagttt tcgttccact gagtgtcaga ccccgtagaa aagatcaaag gatcttc
//
