LOCUS       EcoHIV-NL4-3–mSc       16677 bp    DNA     circular SYN 13-APR-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 16677)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Mar 2, 2025 from SnapGene 8.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16677
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     rep_origin      complement(797..1385)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(join(1556..2347,2348..2416))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 1556 .. 2347 / #ccffcc
                        2: 2348 .. 2416 / #ccffcc / signal sequence
                     Cleavage site after base 2347"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2417..2521)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     LTR             4479..4736
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    4738..4755
                     /label=PDS
                     /note="Primer binding site"
                     /note="color: #ff00ff"
     CDS             4892..6394
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /note="color: #993366"
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             6187..9198
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /note="color: #993366"
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGFDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    8883..9000
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     CDS             9143..9721
                     /codon_start=1
                     /label=vif
                     /note="color: #993366"
                     /translation="MENRWQVMIVWQVDRMRINTWKRLVKHHMYISRKAKDWFYRHHYE
                     STNPKISSEVHIPLGDAKLVITTYWGLHTGERDWHLGQGVSIEWRKKRYSTQVDPDLAD
                     QLIHLHYFDCFSESAIRNTILGRIVSPRCEYQAGHNKVGSLQYLALAALIKPKQIKPPL
                     PSVRKLTEDRWNKPQKTKGHRGSHTMNGH"
     CDS             9661..9951
                     /codon_start=1
                     /label=vpr
                     /note="color: #a6acb3"
                     /translation="MEQAPEDQGPQREPYNEWTLELLEELKSEAVRHFPRIWLHNLGQH
                     IYETYGDTWAGVEAIIRILQQLLFIHFRIGCRHSRIGVTRQRRARNGASRS"
     CDS             join(9932..10146,13026..13071)
                     /codon_start=1
                     /label=Tat
                     /note="This feature has 2 segments:
                        1:  9932 .. 10146 / #a6acb3 / Tat
                        2: 13026 .. 13071 / #a6acb3 / Tat"
                     /translation="MEPVDPRLEPWKHPGSQPKTACTNCYCKKCCFHCQVCFMTKALGI
                     SYGRKKRRQRRRAHQNSQTHQASLSKQPTSQSRGDPTGPKE"
     CDS             join(10071..10146,13026..13300)
                     /codon_start=1
                     /label=rev
                     /note="This feature has 2 segments:
                        1: 10071 .. 10146 / #a6acb3
                        2: 13026 .. 13300 / #a6acb3"
                     /translation="MAGRSGDSDEELIRTVRLIKLLYQSNPPPNPEGTRQARRNRRRRW
                     RERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILV
                     ESPTVLESGTKE"
     CDS             10323..12407
                     /codon_start=1
                     /label=gp80-MoLV-eco
                     /note="color: #993366"
                     /translation="MRVKEKYQHLWRWGWKWGTMLLGILMICSAARSTLSKPLKNKVNP
                     RGPLIPLILLMLRGVSTASPGSSPHQVYNITWEVTNGDRETVWATSGNHPLWTWWPDLT
                     PDLCMLAHHGPSYWGLEYQSPFSSPPGPPCCSGGSSPGCSRDCEEPLTSLTPRCNTAWN
                     RLKLDQTTHKSNEGFYVCPGPHRPRESKSCGGPDSFYCAYWGCETTGRAYWKPSSSWDF
                     ITVNNNLTSDQAVQVCKDNKWCNPLVIRFTDAGRRVTSWTTGHYWGLRLYVSGQDPGLT
                     FGIRLRYQNLGPRVPIGPNPVLADQQPLSKPKPVKSPSVTKPPSGTPLSPTQLPPAGTE
                     NRLLNLVDGAYQALNLTSPDKTQECWLCLVAGPPYYEGVAVLGTYSNHTSAPANCSVAS
                     QHKLTLSEVTGQGLCIGAVPKTHQALCNTTQTSSRGSYYLVAPTGTMWACSTGLTPCIS
                     TTILNLTTDYCVLVELWPRVTYHSPSYVYGLFERSNRHKREPVSLTLALLLGGLTMGGI
                     AAGIGTGTTALMATQQFQQLQAAVQDDLREVEKSISNLEKSLTSLSEVVLQNRRGLDLL
                     FLKEGGLCAALKEECCFYADHTGLVRDSMAKLRERLNQRQKLFESTQGWFEGLFNRSPW
                     FTTLISTIMGPLIVLLMILLFGPYILNRLVQFVKDRISVVQALVLTQQYHQLKPIEYEP
                     "
     misc_feature    12416..12649
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     CDS             12834..12878
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #cc99b2"
                     /translation="KNEQELLELDKWASL"
     CDS             13172..13198
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /note="color: #993366"
                     /translation="LPPLERLTL"
     CDS             13444..14139
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /note="color: #ff0000"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     misc_feature    14168..14740
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="color: #a6acb3"
     misc_feature    14190..14742
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="color: #a6acb3"
     misc_feature    14242..14741
                     /label=CITE
                     /note="CITE enhancer"
                     /note="color: #cc0000; direction: RIGHT"
     CDS             14742..15113
                     /codon_start=1
                     /label=nef
                     /note="color: #993366"
                     /translation="MGGKWSKSSVIGWPAVRERMRRAEPAADGVGAVSRDLEKHGAITS
                     SNTAANNAACAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQ
                     RRQDILDLWIYHTQGYFPDW"
     LTR             15031..15664
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
ORIGIN
        1 atttttgaga cagggtctca ctctgtcacc taggctggag tgcagtggtg caatcacagc
       61 tcactgcagc ctcaacgtcg taagctcaag cgatcctccc acctcagcct gcctggtagc
      121 tgagactaca agcgacgccc cagttaattt ttgtattttt ggtagaggca gcgttttgcc
      181 gtgtggccct ggctggtctc gaactcctgg gctcaagtga tccagcctca gcctcccaaa
      241 gtgctgggac aaccggggcc agtcactgca cctggcccta aaccataatt tctaatcttt
      301 tggctaattt gttagtccta caaaggcagt ctagtcccca ggcaaaaagg gggtttgttt
      361 cgggaaaggg ctgttactgt ctttgtttca aactataaac taagttcctc ctaaacttag
      421 ttcggcctac acccaggaat gaacaaggag agcttggagg ttagaagcac gatggaattg
      481 gttaggtcag atctctttca ctgtctgagt tataattttg caatggtggt tcaaagactg
      541 cccgcttctg acaccagtcg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      601 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      661 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      721 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      781 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
      841 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      901 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      961 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1021 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1081 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1141 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1201 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1261 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1321 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1381 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1441 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1501 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     1561 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1621 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     1681 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     1741 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1801 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1861 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1921 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1981 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2041 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2101 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2161 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2221 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2281 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2341 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2401 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     2461 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     2521 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     2581 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     2641 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     2701 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     2761 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     2821 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
     2881 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccaggggagg cagagattgc
     2941 agtaagctga gatcgcagca ctgcactcca gcctgggcga cagagtaaga ctctgtctca
     3001 aaaataaaat aaataaatca atcagatatt ccaatctttt cctttattta tttatttatt
     3061 ttctattttg gaaacacagt ccttccttat tccagaatta cacatatatt ctatttttct
     3121 ttatatgctc cagttttttt tagaccttca cctgaaatgt gtgtatacaa aatctaggcc
     3181 agtccagcag agcctaaagg taaaaaataa aataataaaa aataaataaa atctagctca
     3241 ctccttcaca tcaaaatgga gatacagctg ttagcattaa ataccaaata acccatcttg
     3301 tcctcaataa ttttaagcgc ctctctccac cacatctaac tcctgtcaaa ggcatgtgcc
     3361 ccttccgggc gctctgctgt gctgccaacc aactggcatg tggactctgc agggtcccta
     3421 actgccaagc cccacagtgt gccctgaggc tgccccttcc ttctagcggc tgcccccact
     3481 cggctttgct ttccctagtt tcagttactt gcgttcagcc aaggtctgaa actaggtgcg
     3541 cacagagcgg taagactgcg agagaaagag accagcttta cagggggttt atcacagtgc
     3601 accctgacag tcgtcagcct cacagggggt ttatcacatt gcaccctgac agtcgtcagc
     3661 ctcacagggg gtttatcaca gtgcaccctt acaatcattc catttgattc acaatttttt
     3721 tagtctctac tgtgcctaac ttgtaagtta aatttgatca gaggtgtgtt cccagagggg
     3781 aaaacagtat atacagggtt cagtactatc gcatttcagg cctccacctg ggtcttggaa
     3841 tgtgtccccc gaggggtgat gactacctca gttggatctc cacaggtcac agtgacacaa
     3901 gataaccaag acacctccca aggctaccac aatgggccgc cctccacgtg cacatggccg
     3961 gaggaactgc catgtcggag gtgcaagcac acctgcgcat cagagtcctt ggtgtggagg
     4021 gagggaccag cgcagcttcc agccatccac ctgatgaaca gaacctaggg aaagccccag
     4081 ttctacttac accaggaaag gctggaaggg ctaatttggt cccaaaaaag acaagagatc
     4141 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
     4201 gggccaggga tcagatatcc actgaccttt ggatggtgct tcaagttagt accagttgaa
     4261 ccagagcaag tagaagaggc caatgaagga gagaacaaca gcttgttaca ccctatgagc
     4321 cagcatggga tggaggaccc ggagggagaa gtattagtgt ggaagtttga cagcctccta
     4381 gcatttcgtc acatggcccg agagctgcat ccggagtact acaaagactg ctgacatcga
     4441 gctttctaca agggactttc cgctggggac tttccaggga ggtgtggcct gggcgggact
     4501 ggggagtggc gagccctcag atgctacata taagcagctg ctttttgcct gtactgggtc
     4561 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     4621 taagcctcaa taaagcttgc cttgagtgct caaagtagtg tgtgcccgtc tgttgtgtga
     4681 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     4741 cgcccgaaca gggacttgaa agcgaaagta aagccagagg agatctctcg acgcaggact
     4801 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     4861 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcgg tattaagcgg
     4921 gggagaatta gataaatggg aaaaaattcg gttaaggcca gggggaaaga aacaatataa
     4981 actaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     5041 tttagagaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     5101 aggatcagaa gaacttagat cattatataa tacaatagca gtcctctatt gtgtgcatca
     5161 aaggatagat gtaaaagaca ccaaggaagc cttagataag atagaggaag agcaaaacaa
     5221 aagtaagaaa aaggcacagc aagcagcagc tgacacagga aacaacagcc aggtcagcca
     5281 aaattaccct atagtgcaga acctccaggg gcaaatggta catcaggcca tatcacctag
     5341 aactttaaat gcatgggtaa aagtagtaga agagaaggct ttcagcccag aagtaatacc
     5401 catgttttca gcattatcag aaggagccac cccacaagat ttaaatacca tgctaaacac
     5461 agtgggggga catcaagcag ccatgcaaat gttaaaagag accatcaatg aggaagctgc
     5521 agaatgggat agattgcatc cagtgcatgc agggcctatt gcaccaggcc agatgagaga
     5581 accaagggga agtgacatag caggaactac tagtaccctt caggaacaaa taggatggat
     5641 gacacataat ccacctatcc cagtaggaga aatctataaa agatggataa tcctgggatt
     5701 aaataaaata gtaagaatgt atagccctac cagcattctg gacataagac aaggaccaaa
     5761 ggaacccttt agagactatg tagaccgatt ctataaaact ctaagagccg agcaagcttc
     5821 acaagaggta aaaaattgga tgacagaaac cttgttggtc caaaatgcga acccagattg
     5881 taagactatt ttaaaagcat tgggaccagg agcgacacta gaagaaatga tgacagcatg
     5941 tcagggagtg gggggacccg gccataaagc aagagttttg gctgaagcaa tgagccaagt
     6001 aacaaatcca gctaccataa tgatacagaa aggcaatttt aggaaccaaa gaaagactgt
     6061 taagtgtttc aattgtggca aagaagggca catagccaaa aattgcaggg cccctaggaa
     6121 aaagggctgt tggaaatgtg gaaaggaagg acaccaaatg aaagattgta ctgagagaca
     6181 ggctaatttt ttagggaaga tctggccttc ccacaaggga aggccaggga attttcttca
     6241 gagcagacca gagccaacag ccccaccaga agagagcttc aggtttgggg aagagacaac
     6301 aactccctct cagaagcagg agccgataga caaggaactg tatcctttag cttccctcag
     6361 atcactcttt ggcagcgacc cctcgtcaca ataaagatag gggggcaatt aaaggaagct
     6421 ctattagata caggagcaga tgatacagta ttagaagaaa tgaatttgcc aggaagatgg
     6481 aaaccaaaaa tgataggggg aattggaggt tttatcaaag taagacagta tgatcagata
     6541 ctcatagaaa tctgcggaca taaagctata ggtacagtat tagtaggacc tacacctgtc
     6601 aacataattg gaagaaatct gttgactcag attggctgca ctttaaattt tcccattagt
     6661 cctattgaga ctgtaccagt aaaattaaag ccaggaatgg atggcccaaa agttaaacaa
     6721 tggccattga cagaagaaaa aataaaagca ttagtagaaa tttgtacaga aatggaaaag
     6781 gaaggaaaaa tttcaaaaat tgggcctgaa aatccataca atactccagt atttgccata
     6841 aagaaaaaag acagtactaa atggagaaaa ttagtagatt tcagagaact taataagaga
     6901 actcaagatt tctgggaagt tcaattagga ataccacatc ctgcagggtt aaaacagaaa
     6961 aaatcagtaa cagtactgga tgtgggcgat gcatattttt cagttccctt agataaagac
     7021 ttcaggaagt atactgcatt taccatacct agtataaaca atgagacacc agggattaga
     7081 tatcagtaca atgtgcttcc acagggatgg aaaggatcac cagcaatatt ccagtgtagc
     7141 atgacaaaaa tcttagagcc ttttagaaaa caaaatccag acatagtcat ctatcaatac
     7201 atggatgatt tgtatgtagg atttgactta gaaatagggc agcatagaac aaaaatagag
     7261 gaactgagac aacatctgtt gaggtgggga tttaccacac cagacaaaaa acatcagaaa
     7321 gaacctccat tcctttggat gggttatgaa ctccatcctg ataaatggac agtacagcct
     7381 atagtgctgc cagaaaagga cagctggact gtcaatgaca tacagaaatt agtgggaaaa
     7441 ttgaattggg caagtcagat ttatgcaggg attaaagtaa ggcaattatg taaacttctt
     7501 aggggaacca aagcactaac agaagtagta ccactaacag aagaagcaga gctagaactg
     7561 gcagaaaaca gggagattct aaaagaaccg gtacatggag tgtattatga cccatcaaaa
     7621 gacttaatag cagaaataca gaagcagggg caaggccaat ggacatatca aatttatcaa
     7681 gagccattta aaaatctgaa aacaggaaag tatgcaagaa tgaagggtgc ccacactaat
     7741 gatgtgaaac aattaacaga ggcagtacaa aaaatagcca cagaaagcat agtaatatgg
     7801 ggaaagactc ctaaatttaa attacccata caaaaggaaa catgggaagc atggtggaca
     7861 gagtattggc aagccacctg gattcctgag tgggagtttg tcaatacccc tcccttagtg
     7921 aagttatggt accagttaga gaaagaaccc ataataggag cagaaacttt ctatgtagat
     7981 ggggcagcca atagggaaac taaattagga aaagcaggat atgtaactga cagaggaaga
     8041 caaaaagttg tccccctaac ggacacaaca aatcagaaga ctgagttaca agcaattcat
     8101 ctagctttgc aggattcggg attagaagta aacatagtga cagactcaca atatgcattg
     8161 ggaatcattc aagcacaacc agataagagt gaatcagagt tagtcagtca aataatagag
     8221 cagttaataa aaaaggaaaa agtctacctg gcatgggtac cagcacacaa aggaattgga
     8281 ggaaatgaac aagtagataa attggtcagt gctggaatca ggaaagtact atttttagat
     8341 ggaatagata aggcccaaga agaacatgag aaatatcaca gtaattggag agcaatggct
     8401 agtgatttta acctaccacc tgtagtagca aaagaaatag tagccagctg tgataaatgt
     8461 cagctaaaag gggaagccat gcatggacaa gtagactgta gcccaggaat atggcagcta
     8521 gattgtacac atttagaagg aaaagttatc ttggtagcag ttcatgtagc cagtggatat
     8581 atagaagcag aagtaattcc agcagagaca gggcaagaaa cagcatactt cctcttaaaa
     8641 ttagcaggaa gatggccagt aaaaacagta catacagaca atggcagcaa tttcaccagt
     8701 actacagtta aggccgcctg ttggtgggcg gggatcaagc aggaatttgg cattccctac
     8761 aatccccaaa gtcaaggagt aatagaatct atgaataaag aattaaagaa aattatagga
     8821 caggtaagag atcaggctga acatcttaag acagcagtac aaatggcagt attcatccac
     8881 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     8941 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     9001 cgggtttatt acagggacag cagagatcca gtttggaaag gaccagcaaa gctcctctgg
     9061 aaaggtgaag gggcagtagt aatacaagat aatagtgaca taaaagtagt gccaagaaga
     9121 aaagcaaaga tcatcaggga ttatggaaaa cagatggcag gtgatgattg tgtggcaagt
     9181 agacaggatg aggattaaca catggaaaag attagtaaaa caccatatgt atatttcaag
     9241 gaaagctaag gactggtttt atagacatca ctatgaaagt actaatccaa aaataagttc
     9301 agaagtacac atcccactag gggatgctaa attagtaata acaacatatt ggggtctgca
     9361 tacaggagaa agagactggc atttgggtca gggagtctcc atagaatgga ggaaaaagag
     9421 atatagcaca caagtagacc ctgacctagc agaccaacta attcatctgc actattttga
     9481 ttgtttttca gaatctgcta taagaaatac catattagga cgtatagtta gtcctaggtg
     9541 tgaatatcaa gcaggacata acaaggtagg atctctacag tacttggcac tagcagcatt
     9601 aataaaacca aaacagataa agccaccttt gcctagtgtt aggaaactga cagaggacag
     9661 atggaacaag ccccagaaga ccaagggcca cagagggagc catacaatga atggacacta
     9721 gagcttttag aggaacttaa gagtgaagct gttagacatt ttcctaggat atggctccat
     9781 aacttaggac aacatatcta tgaaacttac ggggatactt gggcaggagt ggaagccata
     9841 ataagaattc tgcaacaact gctgtttatc catttcagaa ttgggtgtcg acatagcaga
     9901 ataggcgtta ctcgacagag gagagcaaga aatggagcca gtagatccta gactagagcc
     9961 ctggaagcat ccaggaagtc agcctaaaac tgcttgtacc aattgctatt gtaaaaagtg
    10021 ttgctttcat tgccaagttt gtttcatgac aaaagcctta ggcatctcct atggcaggaa
    10081 gaagcggaga cagcgacgaa gagctcatca gaacagtcag actcatcaag cttctctatc
    10141 aaagcagtaa gtagtacatg taatgcaacc tataatagta gcaatagtag cattagtagt
    10201 agcaataata atagcaatag ttgtgtggtc catagtaatc atagaatata ggaaaatatt
    10261 aagacaaaga aaaatagaca ggttaattga tagactaata gaaagagcag aagacagtgg
    10321 caatgagagt gaaggagaag tatcagcact tgtggagatg ggggtggaaa tggggcacca
    10381 tgctccttgg gatattgatg atctgtagtg ctgcgcgttc aacgctctca aaacccctta
    10441 aaaataaggt taacccgcga ggccccctaa tccccttaat tcttctgatg ctcagagggg
    10501 tcagtactgc ttcgcccggc tccagtcctc atcaagtcta taatatcacc tgggaggtaa
    10561 ccaatggaga tcgggagacg gtatgggcaa cttctggcaa ccaccctctg tggacctggt
    10621 ggcctgacct taccccagat ttatgtatgt tagcccacca tggaccatct tattgggggc
    10681 tagaatatca atcccctttt tcttctcccc cggggccccc ttgttgctca gggggcagca
    10741 gcccaggctg ttccagagac tgcgaagaac ctttaacctc cctcacccct cggtgcaaca
    10801 ctgcctggaa cagactcaag ctagaccaga caactcataa atcaaatgag ggattttatg
    10861 tttgccccgg gccccaccgc ccccgagaat ccaagtcatg tgggggtcca gactccttct
    10921 actgtgccta ttggggctgt gagacaaccg gtagagctta ctggaagccc tcctcatcat
    10981 gggatttcat cacagtaaac aacaatctca cctctgacca ggctgtccag gtatgcaaag
    11041 ataataagtg gtgcaacccc ttagttattc ggtttacaga cgccgggaga cgggttactt
    11101 cctggaccac aggacattac tggggcttac gtttgtatgt ctccggacaa gatccagggc
    11161 ttacatttgg gatccgactc agataccaaa atctaggacc ccgcgtccca atagggccaa
    11221 accccgttct ggcagaccaa cagccactct ccaagcccaa acctgttaag tcgccttcag
    11281 tcaccaaacc acccagtggg actcctctct cccctaccca acttccaccg gcgggaacgg
    11341 aaaataggct gctaaactta gtagacggag cctaccaagc cctcaacctc accagtcctg
    11401 acaaaaccca agagtgctgg ttgtgtctag tagcgggacc cccctactac gaaggggttg
    11461 ccgtcctggg tacctactcc aaccatacct ctgctccagc caactgctcc gtggcctccc
    11521 aacacaagtt gaccctgtcc gaagtgaccg gacagggact ctgcatagga gcagttccca
    11581 aaacacatca ggccctatgt aataccaccc agacaagcag tcgagggtcc tattatctag
    11641 ttgcccctac aggtaccatg tgggcttgta gtaccgggct tactccatgc atctccacca
    11701 ccatactgaa ccttaccact gattattgtg ttcttgtcga actctggcca agagtcacct
    11761 atcattcccc cagctatgtt tacggcctgt ttgagagatc caaccgacac aaaagagaac
    11821 cggtgtcgtt aaccctggcc ctattattgg gtggactaac catgggggga attgccgctg
    11881 gaataggaac agggactact gctctaatgg ccactcagca attccagcag ctccaagccg
    11941 cagtacagga tgatctcagg gaggttgaaa aatcaatctc taacctagaa aagtctctca
    12001 cttccctgtc tgaagttgtc ctacagaatc gaaggggcct agacttgtta tttctaaaag
    12061 aaggagggct gtgtgctgct ctaaaagaag aatgttgctt ctatgcggac cacacaggac
    12121 tagtgagaga cagcatggcc aaattgagag agaggcttaa tcagagacag aaactgtttg
    12181 agtcaactca aggatggttt gagggactgt ttaacagatc cccttggttt accaccttga
    12241 tatctaccat tatgggaccc ctcattgtac tcctaatgat tttgctcttc ggaccctaca
    12301 ttcttaatcg attagtccaa tttgttaaag acaggatatc agtggtccag gctctagttt
    12361 tgactcaaca atatcaccag ctgaagccta tagagtacga gccataggtg ggaataggag
    12421 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
    12481 tgacggtaca ggccagacaa ttattgtctg atatagtgca gcagcagaac aatttgctga
    12541 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aaacagctcc
    12601 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
    12661 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
    12721 aatctctgga acagatttgg aataacatga cctggatgga gtgggacaga gaaattaaca
    12781 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
    12841 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
    12901 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
    12961 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
    13021 ttcagaccca cctcccaatc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
    13081 gtggagagag agacagagac agatccattc gattagtgaa cggatcctta gcacttatct
    13141 gggacgatct gcggagcctg tgcctcttca gctaccaccg cttgagagac ttactcttga
    13201 ttgtaacgag gattgtggaa cttctgggac gcagggggtg ggaagccctc aaatattggt
    13261 ggaatctcct acagtattgg agtcaggaac taaagaatag tgctgttaac ttgctcaatg
    13321 ccacagccat agcagtagct gaggggacag atagggttat agaagtatta caagcagctt
    13381 atagagctat tcgccacata cctagaagaa taagacaggg cttggaaagg attttgctat
    13441 aagatggtga gcaagggcga ggcagtgatc aaggagttca tgcggttcaa ggtgcacatg
    13501 gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
    13561 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttctcctgg
    13621 gacatcctgt cccctcagtt catgtacggc tccagggcct tcaccaagca ccccgccgac
    13681 atccccgact actataagca gtccttcccc gagggcttca agtgggagcg cgtgatgaac
    13741 ttcgaggacg gcggcgccgt gaccgtgacc caggacacct ccctggagga cggcaccctg
    13801 atctacaagg tgaagctccg cggcaccaac ttccctcctg acggccccgt aatgcagaag
    13861 aagacaatgg gctgggaagc gtccaccgag cggttgtacc ccgaggacgg cgtgctgaag
    13921 ggcgacatta agatggccct gcgcctgaag gacggcggcc ggtacctggc ggacttcaag
    13981 accacctaca aggccaagaa gcccgtgcag atgcccggcg cctacaacgt cgaccgcaag
    14041 ttggacatca cctcccacaa cgaggactac accgtggtgg aacagtacga acgctccgag
    14101 ggccgccact ccaccggcgg catggacgag ctgtacaagt aaagcggccg cactagtgat
    14161 aattccgccc ctctccctcc ccccccctaa cgttactggc cgaagccgct tggaataagg
    14221 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
    14281 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
    14341 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
    14401 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
    14461 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
    14521 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
    14581 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
    14641 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
    14701 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatgggtggc aagtggtcaa
    14761 aaagtagtgt gattggatgg cctgctgtaa gggaaagaat gagacgagct gagccagcag
    14821 cagatggggt gggagcagta tctcgagacc tagaaaaaca tggagcaatc acaagtagca
    14881 atacagcagc taacaatgct gcttgtgcct ggctagaagc acaagaggag gaagaggtgg
    14941 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
    15001 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caaagaagac
    15061 aagatatcct tgatttgtgg atttaccaca cacaaggcta cttccctgat tggcagaact
    15121 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
    15181 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
    15241 ctgtgagcct gcatggaatg gatgaccctg agagagaagt gttagagtgg aggtttgaca
    15301 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
    15361 gacatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
    15421 gcgggactgg ggagtggcga gccctcagat gctgcatata agcagctgct ttttgcctgt
    15481 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
    15541 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
    15601 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
    15661 agcacccagg aggtagaggt tgcagtgagc caagatcgcg ccactgcatt ccagcctggg
    15721 caagaaaaca agactgtcta aaataataat aataagttaa gggtattaaa tatatttata
    15781 catggaggtc ataaaaatat atatatttgg gctgggcgca gtggctcaca cctgcgcccg
    15841 gccctttggg aggccgaggc aggtggatca cctgagtttg ggagttccag accagcctga
    15901 ccaacatgga gaaacccctt ctctgtgtat ttttagtaga ttttatttta tgtgtatttt
    15961 attcacaggt atttctggaa aactgaaact gtttttcctc tactctgata ccacaagaat
    16021 catcagcaca gaggaagact tctgtgatca aatgtggtgg gagagggagg ttttcaccag
    16081 cacatgagca gtcagttctg ccgcagactc ggcgggtgtc cttcggttca gttccaacac
    16141 cgcctgcctg gagagaggtc agaccacagg gtgagggctc agtccccaag acataaacac
    16201 ccaagacata aacacccaac aggtccaccc cgcctgctgc ccaggcagag ccgattcacc
    16261 aagacgggaa ttaggataga gaaagagtaa gtcacacaga gccggctgtg cgggagaacg
    16321 gagttctatt atgactcaaa tcagtctccc caagcattcg gggatcagag tttttaagga
    16381 taacttagtg tgtagggggc cagtgagttg gagatgaaag cgtagggagt cgaaggtgtc
    16441 cttttgcgcc gagtcagttc ctgggtgggg gccacaagat cggatgagcc agtttatcaa
    16501 tccgggggtg ccagctgatc catggagtgc agggtctgca aaatatctca agcactgatt
    16561 gatcttaggt tttacaatag tgatgttacc ccaggaacaa tttggggaag gtcagaatct
    16621 tgtagcctgt agctgcatga ctcctaaacc ataatttctt ttttgttttt ttttttt
//
