LOCUS       pCAGEN_NES_V5_eMagAF_C7 6134 bp ds-DNA     circular     26-FEB-2025
DEFINITION  .
KEYWORDS    "marker:SV40pA-R, rbglobpA-R, AmpR, Bglob-pA-R, Amp-R"
FEATURES             Location/Qualifiers
     source          1..6134
                     /label="source"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     enhancer        4..383
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(198..218)
                     /label="WQLI11 (QB4767) - bad for seq PX459"
                     /note="sequence: CCGTAAATACTCCACCCATTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(283..300)
                     /label="WQLI12 (QB4768)"
                     /note="sequence: CGGGCCATTTACCGTCAT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     intron          661..1677
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          1336..1359
                     /label="pCX-cMyc-5 (QB4156)"
                     /note="sequence: GCGAGCCGCAGCCATTGCCTTTTA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     1601..1623
                     /label="pCAGGS-5"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1601..1623
                     /label="oAFX083 pCAGGS-5"
                     /note="sequence: ggttcggcttctggcgtgtgacc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1632..1653
                     /label="QB1272 CAG-1F (RF GP0061 CAG-1F)"
                     /note="sequence: TAGAGCCTCTGCTAACCATGTT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1640..1659
                     /label="QB4180"
                     /note="sequence: CTGCTAACCATGTTCATGCC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1646..1663
                     /label="QB4625 (RF GP0115 pCAGGs_F)"
                     /note="sequence: ACCATGTTCATGCCTTCT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1684..1704
                     /label="oAFX339 pCAGEN colony PCR F"
                     /note="sequence: ggcaacgtgctggttattgtg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     1685..1704
                     /label="pCAG-F"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1712..1736
                     /label="XhoI_Hom-F"
                     /note="sequence: atcattttggcaaagaattcctcga"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1712..1736
                     /label="XhoI.Gibson.5'.hom"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1712..1761
                     /label="oAFX171 XhoI5hom.ATG.NES.F"
                     /note="sequence: atcattttggcaaagaattcctcgaATGCTGCAGAAGAAGCTGGAGGAGC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1712..1769
                     /label="oAFX194 noMag.C100.F3"
                     /note="sequence: atcattttggcaaagaattcctcgaATGCTGCAGAAGAAGCTGGAGGAGCTGGAACTG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             1737..3059
                     /label="Translation 1737-3059"
                     /translation="MLQKKLEELELGSSGKPIPNPLLGLDSTGSSGHTLYAPGGYDIMGYLDQIANRPNPQVELGPVDLSCALILCDLKQKDTPIVYASEAFLEMTGYNRHEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTIFTIKKAIDRNAEVQVEVVNFKKNGQRFVNFLTIIPVRDETGEYRYSIGFQCETEGSGSGGSGSGGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK*"
     misc_feature    1740..1769
                     /label="MEK1.NES"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1746..1805
                     /label="oAFX193 noMag.C100.F2"
                     /note="sequence: AAGAAGCTGGAGGAGCTGGAACTGggcTCTTCCGGTAAGCCTATCCCTAACCCTCTCCTC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1759..1818
                     /label="M12_NES_V5"
                     /note="sequence: AGCTGGAACTGggcTCTTCCGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1770..1778
                     /label="GSS"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1779..1820
                     /label="V5"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1821..1829
                     /label="GSS"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1830..2282
                     /label="eMagAF"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1830..2282
                     /label="eMagAF no start codon"
     primer          1833..1857
                     /label="M2_Mag_For"
                     /note="sequence: CACACTCTTTACGCCCCTGGAGGAT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(2096..2146)
                     /label="oAFX422 eMagAF R"
                     /note="sequence: CGGTCGATGGCCTTCTTGATGGTGAAGATCGTGTTCGAGTCCACATATTTG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2122..2168
                     /label="oAFX423 eMagAF F"
                     /note="sequence: TCACCATCAAGAAGGCCATCGACCGGAACGCCGAGGTCCAGGTGGAG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2283..2302
                     /label="oAFX452 2xGSGSG F2"
                     /note="sequence: ggcAGTggaTCCggcggcAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2283..2312
                     /label="2xGSGSG linker"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2285..2303)
                     /label="oAFX420 2xGSGSG R"
                     /note="sequence: ACTgccgccGGAtccACTg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2290..2312)
                     /label="oAFX453 2xGSGSG R2"
                     /note="sequence: gccGGAtccACTgccgccGGAtc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2291..2308
                     /label="oAFX421 2xGSGSG F"
                     /note="sequence: aTCCggcggcAGTggaTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2313..3056
                     /label="BirA C74 fragment (no stop codon)"
     CDS             2313..3059
                     /label="C74"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2474..2494
                     /label="(52) BirA_cat_domain_internal_seq_F"
                     /note="sequence: TGGCGCAAATCTGTACCTTTC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2474..2494
                     /label="(52) BirA_cat_domain_internal_seq_F"
                     /note="sequence: TGGCGCAAATCTGTACCTTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2483..2513)
                     /label="M22_BirAN_Rev"
                     /note="sequence: TTCCAGCCTCCAGAACATAGAAAGGTACAGA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(2489..2516)
                     /label="S10_BirAN_140_Rev"
                     /note="sequence: CTGTTCCAGCCTCCAGAACATAGAAAGG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2755..2772
                     /label="(DS) BirA_Seq_F"
                     /note="sequence: AAGGCTGGATCACCTTGC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2755..2772
                     /label="(DS) BirA_Seq_F"
                     /note="sequence: AAGGCTGGATCACCTTGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2837..2861)
                     /label="S1_Split_Nterm_256"
                     /note="sequence: CTCCTGCTCGAACAGTTCCAGGGCG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(3027..3084)
                     /label="M14_BirAC_Xho1"
                     /note="sequence: aggagtgcggccgcgatatcctcgaTCACTTCTCTGCAGATCTAAGGGAGATTTCCCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(3043..3083)
                     /label="C233-Reverse"
                     /note="sequence: ggagtgcggccgcgatatcctcgaTCACTTCTCTGCAGATC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3060..3084
                     /label="XhoI.Gibson.3'.hom"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3060..3084)
                     /label="XhoI_Hom-R"
                     /note="sequence: aggagtgcggccgcgatatcctcga"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3105..3123)
                     /label="oAFX338 pCAGEN colony PCR R"
                     /note="sequence: cacaccagccaccaccttc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(3156..3175)
                     /label="Bglob-pA-R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(3197..3215)
                     /label="pCAGGs_R (QB4626)"
                     /note="sequence: GAAGTCAGATGCTCAAGGG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(3197..3215)
                     /label="GP0116 pCAGGs_R"
                     /note="sequence: GAAGTCAGATGCTCAAGGG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(3202..3222)
                     /label="pCX-cMyc-3 (QB4157)"
                     /note="sequence: TTAGCCAGAAGTCAGATGCTC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     polyA_site      3221..3276
                     /label="-beta-globin poly(A) signal"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(3275..3294)
                     /label="rbglobpA-R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(3275..3294)
                     /label="pYD5-R (QB1411)"
                     /note="sequence: CCCATATGTCCTTCCGAGTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(3637..3653)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(3637..3653)
                     /label="M13 Reverse"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3637..3653)
                     /label="QB0048 M13-Rev"
                     /note="sequence: CAGGAAACAGCTATGAC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(3650..3672)
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     protein_bind    3661..3677
                     /label="lac operator"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(3672..3688)
                     /label="QB0136 M13Reverse(-40)"
                     /note="sequence: GTTGTGTGGAATTGTGA"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        complement(3685..3715)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    3730..3751
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        3810..4005
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      3856..3991
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     3918..3937
                     /label="SV40pro-F"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     polyA_signal    4011..4145
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(4048..4067)
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     4102..4121
                     /label="EBV-rev"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(4213..4230)
                     /label="L4440"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      complement(4384..4972)
                     /label="ori"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(4464..4483)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(5143..6003)
                     /label="AmpR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     5766..5785
                     /label="Amp-R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          5933..5952
                     /label="oAFX429 Eu-Myc JAX-14378 R"
                     /note="sequence: TGAGCAAAAACAGGAAGGCA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        complement(6004..6108)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggcgcgcg ccrggggggg gggggggggg gggggggggg
      541 gggggggggg gggggggggg kgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
      721 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
      781 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
      841 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
      901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
      961 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
     1321 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1381 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     1441 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     1561 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     1621 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     1681 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt cctcgaATGC
     1741 TGCAGAAGAA GCTGGAGGAG CTGGAACTGg gcTCTTCCGG TAAGCCTATC CCTAACCCTC
     1801 TCCTCGGTCT CGATTCTACG ggaTCCTCTG GACACACTCT TTACGCCCCT GGAGGATACG
     1861 ACATTATGGG ATATTTGGAT CAGATTGCGA ACCGCCCAAA CCCTCAGGTC GAACTGGGGC
     1921 CTGTGGACCT GTCATGTGCC CTGATCCTGT GCGATCTGAA GCAAAAGGAC ACTCCGATCG
     1981 TCTACGCCTC GGAAGCCTTC TTGGAGATGA CCGGATACAA CAGACATGAG GTGCTCGGCA
     2041 GGAACTGCAG ATTCCTGCAG TCCCCCGACG GGATGGTGAA ACCAAAGTCG ACTCGCAAAT
     2101 ATGTGGACTC GAACACGATC TTCACCATCA AGAAGGCCAT CGACCGGAAC GCCGAGGTCC
     2161 AGGTGGAGGT GGTCAACTTT AAGAAGAACG GCCAGCGGTT CGTGAACTTT CTGACCATCA
     2221 TTCCGGTCCG GGATGAAACC GGAGAGTACA GATACTCCAT CGGATTCCAG TGCGAAACCG
     2281 AAggcAGTgg aTCCggcggc AGTggaTCCg gcGGACAACT GGACGGTGGG AGTGTTGCCG
     2341 TCCTCCCTGT GATCGACTCC ACGAACCAGT ACTTGCTGGA CCGCATCGGC GAGCTTAAAA
     2401 GCGGAGATGC TTGTATCGCT GAATACCAGC AGGCCGGGCG CGGCAGACGG GGCCGGAAGT
     2461 GGTTCTCTCC TTTTGGCGCA AATCTGTACC TTTCTATGTT CTGGAGGCTG GAACAGGGAC
     2521 CAGCAGCCGC AATCGGCTTG TCCCTGGTCA TTGGGATTGT GATGGCCGAA GTTCTCAGAA
     2581 AGCTGGGAGC TGATAAGGTG AGGGTTAAAT GGCCTAACGA TCTGTATTTG CAGGACCGGA
     2641 AACTGGCCGG AATACTGGTG GAGCTGACTG GCAAGACTGG CGACGCCGCT CAGATAGTCA
     2701 TCGGAGCCGG TATAAATATG GCCATGAGGC GCGTCGAGGA ATCCGTGGTG AACCAAGGCT
     2761 GGATCACCTT GCAAGAGGCC GGTATTAACC TGGATAGAAA CACCCTGGCC GCTATGCTGA
     2821 TACGCGAACT GCGCGCCGCC CTGGAACTGT TCGAGCAGGA GGGACTCGCT CCATATCTGA
     2881 GTCGCTGGGA AAAATTGGAT AACTTCATAA ACCGACCCGT GAAGCTGATC ATCGGCGACA
     2941 AGGAAATTTT CGGAATCTCT CGCGGCATCG ATAAACAGGG GGCATTGCTG CTCGAGCAGG
     3001 ATGGGATTAT TAAGCCATGG ATGGGCGGGG AAATCTCCCT TAGATCTGCA GAGAAGTGAt
     3061 cgaggatatc gcggccgcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
     3121 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
     3181 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     3241 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     3301 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     3361 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     3421 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     3481 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     3541 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
     3601 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     3661 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     3721 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3781 gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg
     3841 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3901 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3961 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta
     4021 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     4081 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     4141 ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4201 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4261 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4321 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4381 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4441 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4501 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4561 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4621 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4681 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4741 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4801 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     4861 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4921 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4981 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5041 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5101 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5161 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5221 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5281 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5341 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5401 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5461 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5521 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5581 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5641 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5701 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5761 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5821 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5881 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5941 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     6001 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     6061 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6121 aaaagtgcca cctg
//