LOCUS       pCAGEN_NES_V5_eMagAF_C1 6056 bp ds-DNA     circular     26-FEB-2025
DEFINITION  .
KEYWORDS    "marker:SV40pA-R, rbglobpA-R, AmpR, Bglob-pA-R, Amp-R"
FEATURES             Location/Qualifiers
     enhancer        4..383
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(198..218)
                     /label="WQLI11 (QB4767) - bad for seq PX459"
                     /note="sequence: CCGTAAATACTCCACCCATTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(283..300)
                     /label="WQLI12 (QB4768)"
                     /note="sequence: CGGGCCATTTACCGTCAT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     intron          661..1677
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          1336..1359
                     /label="pCX-cMyc-5 (QB4156)"
                     /note="sequence: GCGAGCCGCAGCCATTGCCTTTTA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     1601..1623
                     /label="pCAGGS-5"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1601..1623
                     /label="oAFX083 pCAGGS-5"
                     /note="sequence: ggttcggcttctggcgtgtgacc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1632..1653
                     /label="QB1272 CAG-1F (RF GP0061 CAG-1F)"
                     /note="sequence: TAGAGCCTCTGCTAACCATGTT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1640..1659
                     /label="QB4180"
                     /note="sequence: CTGCTAACCATGTTCATGCC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1646..1663
                     /label="QB4625 (RF GP0115 pCAGGs_F)"
                     /note="sequence: ACCATGTTCATGCCTTCT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1684..1704
                     /label="oAFX339 pCAGEN colony PCR F"
                     /note="sequence: ggcaacgtgctggttattgtg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     1685..1704
                     /label="pCAG-F"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1712..1736
                     /label="XhoI_Hom-F"
                     /note="sequence: atcattttggcaaagaattcctcga"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1712..1736
                     /label="XhoI.Gibson.5'.hom"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1712..1761
                     /label="oAFX171 XhoI5hom.ATG.NES.F"
                     /note="sequence: atcattttggcaaagaattcctcgaATGCTGCAGAAGAAGCTGGAGGAGC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1712..1769
                     /label="oAFX194 noMag.C100.F3"
                     /note="sequence: atcattttggcaaagaattcctcgaATGCTGCAGAAGAAGCTGGAGGAGCTGGAACTG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             1737..2981
                     /label="full_translation"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MLQKKLEELELGSSGKPIPNPLLGLDSTGSSGHTLYAPGGYDIMGYLDQIANRPNPQVELGPVDLSCALILCDLKQKDTPIVYASEAFLEMTGYNRHEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTIFTIKKAIDRNAEVQVEVVNFKKNGQRFVNFLTIIPVRDETGEYRYSIGFQCETEGSGSGGSGSGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK*"
     misc_feature    1740..1769
                     /label="MEK1.NES"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1746..1805
                     /label="oAFX193 noMag.C100.F2"
                     /note="sequence: AAGAAGCTGGAGGAGCTGGAACTGggcTCTTCCGGTAAGCCTATCCCTAACCCTCTCCTC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1759..1818
                     /label="M12_NES_V5"
                     /note="sequence: AGCTGGAACTGggcTCTTCCGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1770..1778
                     /label="GSS"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1779..1820
                     /label="V5"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1821..1829
                     /label="GSS"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1830..2282
                     /label="eMagAF"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1830..2282
                     /label="eMagAF no start codon"
     primer          1833..1857
                     /label="M2_Mag_For"
                     /note="sequence: CACACTCTTTACGCCCCTGGAGGAT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(2096..2146)
                     /label="oAFX422 eMagAF R"
                     /note="sequence: CGGTCGATGGCCTTCTTGATGGTGAAGATCGTGTTCGAGTCCACATATTTG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2122..2168
                     /label="oAFX423 eMagAF F"
                     /note="sequence: TCACCATCAAGAAGGCCATCGACCGGAACGCCGAGGTCCAGGTGGAG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2283..2302
                     /label="oAFX452 2xGSGSG F2"
                     /note="sequence: ggcAGTggaTCCggcggcAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2283..2312
                     /label="2xGSGSG linker"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2285..2303)
                     /label="oAFX420 2xGSGSG R"
                     /note="sequence: ACTgccgccGGAtccACTg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          2288..2335
                     /label="oAFX465 2xGSGSG C100 F"
                     /note="sequence: TggaTCCggcggcAGTggaTCCggcGAGCTTAAAAGCGGAGATGCTTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2290..2312)
                     /label="oAFX453 2xGSGSG R2"
                     /note="sequence: gccGGAtccACTgccgccGGAtc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2291..2308
                     /label="oAFX421 2xGSGSG F"
                     /note="sequence: aTCCggcggcAGTggaTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2313..2978
                     /label="C100"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2313..2978
                     /label="BirA C100 fragment (no stop codon)"
     primer          2396..2416
                     /label="(52) BirA_cat_domain_internal_seq_F"
                     /note="sequence: TGGCGCAAATCTGTACCTTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2396..2416
                     /label="(52) BirA_cat_domain_internal_seq_F"
                     /note="sequence: TGGCGCAAATCTGTACCTTTC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(2405..2435)
                     /label="M22_BirAN_Rev"
                     /note="sequence: TTCCAGCCTCCAGAACATAGAAAGGTACAGA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(2411..2438)
                     /label="S10_BirAN_140_Rev"
                     /note="sequence: CTGTTCCAGCCTCCAGAACATAGAAAGG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2677..2694
                     /label="(DS) BirA_Seq_F"
                     /note="sequence: AAGGCTGGATCACCTTGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2677..2694
                     /label="(DS) BirA_Seq_F"
                     /note="sequence: AAGGCTGGATCACCTTGC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2759..2783)
                     /label="S1_Split_Nterm_256"
                     /note="sequence: CTCCTGCTCGAACAGTTCCAGGGCG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2949..3006)
                     /label="M14_BirAC_Xho1"
                     /note="sequence: aggagtgcggccgcgatatcctcgaTCACTTCTCTGCAGATCTAAGGGAGATTTCCCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(2965..3005)
                     /label="C233-Reverse"
                     /note="sequence: ggagtgcggccgcgatatcctcgaTCACTTCTCTGCAGATC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2982..3006
                     /label="XhoI.Gibson.3'.hom"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(2982..3006)
                     /label="XhoI_Hom-R"
                     /note="sequence: aggagtgcggccgcgatatcctcga"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3027..3045)
                     /label="oAFX338 pCAGEN colony PCR R"
                     /note="sequence: cacaccagccaccaccttc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(3078..3097)
                     /label="Bglob-pA-R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(3119..3137)
                     /label="pCAGGs_R (QB4626)"
                     /note="sequence: GAAGTCAGATGCTCAAGGG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(3119..3137)
                     /label="GP0116 pCAGGs_R"
                     /note="sequence: GAAGTCAGATGCTCAAGGG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(3124..3144)
                     /label="pCX-cMyc-3 (QB4157)"
                     /note="sequence: TTAGCCAGAAGTCAGATGCTC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     polyA_site      3143..3198
                     /label="-beta-globin poly(A) signal"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(3197..3216)
                     /label="rbglobpA-R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(3197..3216)
                     /label="pYD5-R (QB1411)"
                     /note="sequence: CCCATATGTCCTTCCGAGTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(3559..3575)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(3559..3575)
                     /label="M13 Reverse"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3559..3575)
                     /label="QB0048 M13-Rev"
                     /note="sequence: CAGGAAACAGCTATGAC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(3572..3594)
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     protein_bind    3583..3599
                     /label="lac operator"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(3594..3610)
                     /label="QB0136 M13Reverse(-40)"
                     /note="sequence: GTTGTGTGGAATTGTGA"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        complement(3607..3637)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    3652..3673
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        3732..3927
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      3778..3913
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     3840..3859
                     /label="SV40pro-F"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     polyA_signal    3933..4067
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(3970..3989)
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     4024..4043
                     /label="EBV-rev"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(4135..4152)
                     /label="L4440"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      complement(4306..4894)
                     /label="ori"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(4386..4405)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(5065..5925)
                     /label="AmpR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     5688..5707
                     /label="Amp-R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          5855..5874
                     /label="oAFX429 Eu-Myc JAX-14378 R"
                     /note="sequence: TGAGCAAAAACAGGAAGGCA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        complement(5926..6030)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggcgcgcg ccrggggggg gggggggggg gggggggggg
      541 gggggggggg gggggggggg kgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
      721 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
      781 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
      841 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
      901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
      961 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
     1321 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1381 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     1441 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     1561 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     1621 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     1681 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt cctcgaATGC
     1741 TGCAGAAGAA GCTGGAGGAG CTGGAACTGg gcTCTTCCGG TAAGCCTATC CCTAACCCTC
     1801 TCCTCGGTCT CGATTCTACG ggaTCCTCTG GACACACTCT TTACGCCCCT GGAGGATACG
     1861 ACATTATGGG ATATTTGGAT CAGATTGCGA ACCGCCCAAA CCCTCAGGTC GAACTGGGGC
     1921 CTGTGGACCT GTCATGTGCC CTGATCCTGT GCGATCTGAA GCAAAAGGAC ACTCCGATCG
     1981 TCTACGCCTC GGAAGCCTTC TTGGAGATGA CCGGATACAA CAGACATGAG GTGCTCGGCA
     2041 GGAACTGCAG ATTCCTGCAG TCCCCCGACG GGATGGTGAA ACCAAAGTCG ACTCGCAAAT
     2101 ATGTGGACTC GAACACGATC TTCACCATCA AGAAGGCCAT CGACCGGAAC GCCGAGGTCC
     2161 AGGTGGAGGT GGTCAACTTT AAGAAGAACG GCCAGCGGTT CGTGAACTTT CTGACCATCA
     2221 TTCCGGTCCG GGATGAAACC GGAGAGTACA GATACTCCAT CGGATTCCAG TGCGAAACCG
     2281 AAggcAGTgg aTCCggcggc AGTggaTCCg gcGAGCTTAA AAGCGGAGAT GCTTGTATCG
     2341 CTGAATACCA GCAGGCCGGG CGCGGCAGAC GGGGCCGGAA GTGGTTCTCT CCTTTTGGCG
     2401 CAAATCTGTA CCTTTCTATG TTCTGGAGGC TGGAACAGGG ACCAGCAGCC GCAATCGGCT
     2461 TGTCCCTGGT CATTGGGATT GTGATGGCCG AAGTTCTCAG AAAGCTGGGA GCTGATAAGG
     2521 TGAGGGTTAA ATGGCCTAAC GATCTGTATT TGCAGGACCG GAAACTGGCC GGAATACTGG
     2581 TGGAGCTGAC TGGCAAGACT GGCGACGCCG CTCAGATAGT CATCGGAGCC GGTATAAATA
     2641 TGGCCATGAG GCGCGTCGAG GAATCCGTGG TGAACCAAGG CTGGATCACC TTGCAAGAGG
     2701 CCGGTATTAA CCTGGATAGA AACACCCTGG CCGCTATGCT GATACGCGAA CTGCGCGCCG
     2761 CCCTGGAACT GTTCGAGCAG GAGGGACTCG CTCCATATCT GAGTCGCTGG GAAAAATTGG
     2821 ATAACTTCAT AAACCGACCC GTGAAGCTGA TCATCGGCGA CAAGGAAATT TTCGGAATCT
     2881 CTCGCGGCAT CGATAAACAG GGGGCATTGC TGCTCGAGCA GGATGGGATT ATTAAGCCAT
     2941 GGATGGGCGG GGAAATCTCC CTTAGATCTG CAGAGAAGTG Atcgaggata tcgcggccgc
     3001 actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct
     3061 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc
     3121 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
     3181 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
     3241 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa
     3301 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc
     3361 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt
     3421 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc
     3481 tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc
     3541 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     3601 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     3661 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     3721 ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     3781 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     3841 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     3901 ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt
     3961 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4021 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg
     4081 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     4141 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4201 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4261 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4321 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4381 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4441 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4501 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4561 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4621 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4681 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4741 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4801 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4861 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4921 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4981 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     5041 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     5101 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     5161 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     5221 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     5281 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     5341 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     5401 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     5461 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     5521 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     5581 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5641 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5701 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     5761 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5821 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     5881 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     5941 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     6001 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctg
//