LOCUS       pCAGEN_N195_eMagBF_V5_N 5972 bp ds-DNA     circular     27-FEB-2025
DEFINITION  .
KEYWORDS    "marker:rbglobpA-R, AmpR, SV40pA-R, Bglob-pA-R, Amp-R"
FEATURES             Location/Qualifiers
     source          1..5972
                     /label="source"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     enhancer        4..383
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(198..218)
                     /label="WQLI11 (QB4767) - bad for seq PX459"
                     /note="sequence: CCGTAAATACTCCACCCATTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(283..300)
                     /label="WQLI12 (QB4768)"
                     /note="sequence: CGGGCCATTTACCGTCAT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     intron          661..1677
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          1336..1359
                     /label="pCX-cMyc-5 (QB4156)"
                     /note="sequence: GCGAGCCGCAGCCATTGCCTTTTA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     1601..1623
                     /label="pCAGGS-5"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1601..1623
                     /label="oAFX083 pCAGGS-5"
                     /note="sequence: ggttcggcttctggcgtgtgacc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1632..1653
                     /label="QB1272 CAG-1F (RF GP0061 CAG-1F)"
                     /note="sequence: TAGAGCCTCTGCTAACCATGTT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1640..1659
                     /label="QB4180"
                     /note="sequence: CTGCTAACCATGTTCATGCC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1646..1663
                     /label="QB4625 (RF GP0115 pCAGGs_F)"
                     /note="sequence: ACCATGTTCATGCCTTCT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1684..1704
                     /label="oAFX339 pCAGEN colony PCR F"
                     /note="sequence: ggcaacgtgctggttattgtg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     1685..1704
                     /label="pCAG-F"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1712..1736
                     /label="XhoI_Hom-F"
                     /note="sequence: atcattttggcaaagaattcctcga"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1712..1736
                     /label="XhoI 5' homology"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          1712..1760
                     /label="oAFX161 XhoI5hom.ATG.BirA.F"
                     /note="sequence: atcattttggcaaagaattcctcgaATGAAGGACAACACTGTTCCGCTT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1737..2321
                     /label="N195"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             1737..2897
                     /label="full_translation"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGSGSGGSGSGGHTLYAPGGYDIMGYLRQIRNRPNPQVELGPVDLSCALVLCDLKQKDTPVVYASEAFLEMTGYNRHEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTIFTMKKAIDRNAEVQVEVVNFKKNGQRFVNFLTMIPVRDETGEYRYSIGFQCETEGSSGKPIPNPLLGLDSTGSSLQKKLEELEL*"
     misc_feature    1740..2321
                     /label="BirA N195 fragment (no start codon)"
     primer          2117..2137
                     /label="(52) BirA_cat_domain_internal_seq_F"
                     /note="sequence: TGGCGCAAATCTGTACCTTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2117..2137
                     /label="(52) BirA_cat_domain_internal_seq_F"
                     /note="sequence: TGGCGCAAATCTGTACCTTTC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(2126..2156)
                     /label="M22_BirAN_Rev"
                     /note="sequence: TTCCAGCCTCCAGAACATAGAAAGGTACAGA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(2132..2159)
                     /label="S10_BirAN_140_Rev"
                     /note="sequence: CTGTTCCAGCCTCCAGAACATAGAAAGG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2301..2346)
                     /label="oAFX459 2xGSGSG N195 R"
                     /note="sequence: AtccACTgccgccGGAtccACTgccAGTCTTGCCAGTCAGCTCCAC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2322..2341
                     /label="oAFX452 2xGSGSG F2"
                     /note="sequence: ggcAGTggaTCCggcggcAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2322..2351
                     /label="2xGSGSG linker"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2324..2342)
                     /label="oAFX420 2xGSGSG R"
                     /note="sequence: ACTgccgccGGAtccACTg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2329..2351)
                     /label="oAFX453 2xGSGSG R2"
                     /note="sequence: gccGGAtccACTgccgccGGAtc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2330..2347
                     /label="oAFX421 2xGSGSG F"
                     /note="sequence: aTCCggcggcAGTggaTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2352..2804
                     /label="eMagBF"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2352..2804
                     /label="eMagBF no start codon"
     misc_feature    2805..2813
                     /label="GSS"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2814..2855
                     /label="V5.a"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(2814..2886)
                     /label="M25_V5_NES"
                     /note="sequence: GCTCCTCCAGCTTCTTCTGCAGGCTACTgccCGTAGAATCGAGACCGAGGAGAGGGTTAGGGATAGGCTTACC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2827..2886)
                     /label="oAFX191 N99.noMag.R2"
                     /note="sequence: GCTCCTCCAGCTTCTTCTGCAGGCTACTgccCGTAGAATCGAGACCGAGGAGAGGGTTAG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2856..2864
                     /label="GSS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2865..2894
                     /label="MEK1.NES"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2865..2919)
                     /label="M26_NES_Xho1"
                     /note="sequence: agtgcggccgcgatatcctcgaTCACAGTTCCAGCTCCTCCAGCTTCTTCTGCAG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2898..2922
                     /label="XhoI 3' homology"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(2898..2922)
                     /label="XhoI_Hom-R"
                     /note="sequence: aggagtgcggccgcgatatcctcga"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2943..2961)
                     /label="oAFX338 pCAGEN colony PCR R"
                     /note="sequence: cacaccagccaccaccttc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(2994..3013)
                     /label="Bglob-pA-R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(3035..3053)
                     /label="pCAGGs_R (QB4626)"
                     /note="sequence: GAAGTCAGATGCTCAAGGG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(3035..3053)
                     /label="GP0116 pCAGGs_R"
                     /note="sequence: GAAGTCAGATGCTCAAGGG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(3040..3060)
                     /label="pCX-cMyc-3 (QB4157)"
                     /note="sequence: TTAGCCAGAAGTCAGATGCTC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     polyA_site      3059..3114
                     /label="-beta-globin poly(A) signal"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(3113..3132)
                     /label="rbglobpA-R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(3113..3132)
                     /label="pYD5-R (QB1411)"
                     /note="sequence: CCCATATGTCCTTCCGAGTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(3475..3491)
                     /label="M13 Reverse"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(3475..3491)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(3475..3491)
                     /label="QB0048 M13-Rev"
                     /note="sequence: CAGGAAACAGCTATGAC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(3488..3510)
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     protein_bind    3499..3515
                     /label="lac operator"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(3510..3526)
                     /label="QB0136 M13Reverse(-40)"
                     /note="sequence: GTTGTGTGGAATTGTGA"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        complement(3523..3553)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    3568..3589
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        3648..3843
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      3694..3829
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     3756..3775
                     /label="SV40pro-F"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     polyA_signal    3849..3983
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(3886..3905)
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     3940..3959
                     /label="EBV-rev"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(4051..4068)
                     /label="L4440"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      complement(4222..4810)
                     /label="ori"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(4302..4321)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(4981..5841)
                     /label="AmpR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     5604..5623
                     /label="Amp-R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          5771..5790
                     /label="oAFX429 Eu-Myc JAX-14378 R"
                     /note="sequence: TGAGCAAAAACAGGAAGGCA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        complement(5842..5946)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggcgcgcg ccrggggggg gggggggggg gggggggggg
      541 gggggggggg gggggggggg kgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
      721 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
      781 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
      841 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
      901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
      961 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
     1321 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1381 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     1441 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     1561 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     1621 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     1681 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt cctcgaATGA
     1741 AGGACAACAC TGTTCCGCTT AAGCTGATAG CCCTCCTGGC CAATGGAGAG TTCCACAGCG
     1801 GTGAGCAGCT CGGTGAGACT CTCGGGATGT CTCGCGCCGC TATTAACAAG CACATTCAGA
     1861 CGTTGCGCGA TTGGGGAGTC GACGTGTTCA CAGTACCTGG CAAAGGGTAC TCACTCCCTG
     1921 AGCCTATCCA GTTGCTGAAC GCAAAGCAGA TTCTGGGACA ACTGGACGGT GGGAGTGTTG
     1981 CCGTCCTCCC TGTGATCGAC TCCACGAACC AGTACTTGCT GGACCGCATC GGCGAGCTTA
     2041 AAAGCGGAGA TGCTTGTATC GCTGAATACC AGCAGGCCGG GCGCGGCAGA CGGGGCCGGA
     2101 AGTGGTTCTC TCCTTTTGGC GCAAATCTGT ACCTTTCTAT GTTCTGGAGG CTGGAACAGG
     2161 GACCAGCAGC CGCAATCGGC TTGTCCCTGG TCATTGGGAT TGTGATGGCC GAAGTTCTCA
     2221 GAAAGCTGGG AGCTGATAAG GTGAGGGTTA AATGGCCTAA CGATCTGTAT TTGCAGGACC
     2281 GGAAACTGGC CGGAATACTG GTGGAGCTGA CTGGCAAGAC TggcAGTgga TCCggcggcA
     2341 GTggaTCCgg cGGACATACC CTCTACGCGC CGGGGGGTTA TGACATCATG GGTTACCTCA
     2401 GACAGATCAG AAACCGGCCG AACCCACAAG TGGAGCTGGG ACCCGTCGAC CTCTCCTGCG
     2461 CCCTCGTGCT GTGTGACCTT AAGCAGAAGG ACACCCCTGT GGTGTACGCC TCCGAAGCAT
     2521 TCCTGGAGAT GACCGGGTAC AACAGACACG AAGTGCTGGG ACGGAACTGC CGCTTCCTGC
     2581 AATCCCCGGA TGGAATGGTG AAGCCTAAGT CAACCCGCAA ATACGTGGAC TCCAACACTA
     2641 TCTTCACCAT GAAGAAGGCC ATTGACCGCA ATGCTGAGGT GCAAGTGGAA GTGGTGAACT
     2701 TCAAGAAGAA CGGACAGCGC TTCGTCAACT TCCTGACTAT GATTCCCGTG CGGGACGAAA
     2761 CCGGCGAATA CCGGTACAGC ATCGGGTTTC AGTGCGAGAC TGAGggaTCC TCTGGTAAGC
     2821 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGggcAG TAGCCTGCAG AAGAAGCTGG
     2881 AGGAGCTGGA ACTGTGAtcg aggatatcgc ggccgcactc ctcaggtgca ggctgcctat
     2941 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt
     3001 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
     3061 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
     3121 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
     3181 gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag
     3241 tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt
     3301 tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc
     3361 ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc
     3421 cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata
     3481 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     3541 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     3601 ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag
     3661 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     3721 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     3781 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     3841 aaaaagctaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3901 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3961 atgtatctta tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg
     4021 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     4081 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     4141 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4201 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4261 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4321 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4381 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4441 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4501 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4561 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4621 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     4681 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4741 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4801 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4861 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4921 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4981 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     5041 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     5101 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     5161 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     5221 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5281 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     5341 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     5401 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5461 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5521 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5581 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     5641 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     5701 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     5761 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     5821 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     5881 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     5941 tccgcgcaca tttccccgaa aagtgccacc tg
//