LOCUS       pCAGEN_N256_eMagBF_V5_N 6155 bp ds-DNA     circular     27-FEB-2025
DEFINITION  .
KEYWORDS    "marker:rbglobpA-R, AmpR, SV40pA-R, Bglob-pA-R, Amp-R"
FEATURES             Location/Qualifiers
     source          1..6155
                     /label="source"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     enhancer        4..383
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(198..218)
                     /label="WQLI11 (QB4767) - bad for seq PX459"
                     /note="sequence: CCGTAAATACTCCACCCATTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(283..300)
                     /label="WQLI12 (QB4768)"
                     /note="sequence: CGGGCCATTTACCGTCAT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     intron          661..1677
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          1336..1359
                     /label="pCX-cMyc-5 (QB4156)"
                     /note="sequence: GCGAGCCGCAGCCATTGCCTTTTA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     1601..1623
                     /label="pCAGGS-5"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1601..1623
                     /label="oAFX083 pCAGGS-5"
                     /note="sequence: ggttcggcttctggcgtgtgacc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1632..1653
                     /label="QB1272 CAG-1F (RF GP0061 CAG-1F)"
                     /note="sequence: TAGAGCCTCTGCTAACCATGTT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1640..1659
                     /label="QB4180"
                     /note="sequence: CTGCTAACCATGTTCATGCC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1646..1663
                     /label="QB4625 (RF GP0115 pCAGGs_F)"
                     /note="sequence: ACCATGTTCATGCCTTCT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1684..1704
                     /label="oAFX339 pCAGEN colony PCR F"
                     /note="sequence: ggcaacgtgctggttattgtg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     1685..1704
                     /label="pCAG-F"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1712..1736
                     /label="XhoI_Hom-F"
                     /note="sequence: atcattttggcaaagaattcctcga"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1712..1736
                     /label="XhoI 5' homology"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          1712..1760
                     /label="oAFX161 XhoI5hom.ATG.BirA.F"
                     /note="sequence: atcattttggcaaagaattcctcgaATGAAGGACAACACTGTTCCGCTT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1737..2504
                     /label="N256"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             1737..3080
                     /label="full_translation"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGSGSGGSGSGGHTLYAPGGYDIMGYLRQIRNRPNPQVELGPVDLSCALVLCDLKQKDTPVVYASEAFLEMTGYNRHEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTIFTMKKAIDRNAEVQVEVVNFKKNGQRFVNFLTMIPVRDETGEYRYSIGFQCETEGSSGKPIPNPLLGLDSTGSSLQKKLEELEL*"
     misc_feature    1740..2504
                     /label="BirA N256 fragment (no start codon)"
     primer          2117..2137
                     /label="(52) BirA_cat_domain_internal_seq_F"
                     /note="sequence: TGGCGCAAATCTGTACCTTTC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2117..2137
                     /label="(52) BirA_cat_domain_internal_seq_F"
                     /note="sequence: TGGCGCAAATCTGTACCTTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2126..2156)
                     /label="M22_BirAN_Rev"
                     /note="sequence: TTCCAGCCTCCAGAACATAGAAAGGTACAGA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(2132..2159)
                     /label="S10_BirAN_140_Rev"
                     /note="sequence: CTGTTCCAGCCTCCAGAACATAGAAAGG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2398..2415
                     /label="(DS) BirA_Seq_F"
                     /note="sequence: AAGGCTGGATCACCTTGC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2398..2415
                     /label="(DS) BirA_Seq_F"
                     /note="sequence: AAGGCTGGATCACCTTGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2480..2504)
                     /label="S1_Split_Nterm_256"
                     /note="sequence: CTCCTGCTCGAACAGTTCCAGGGCG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2484..2529)
                     /label="oAFX462 2xGSGSG N256 R"
                     /note="sequence: AtccACTgccgccGGAtccACTgccCTCCTGCTCGAACAGTTCCAG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2484..2543)
                     /label="oAFX489 eMagBF 2xGSGSG N256 R"
                     /note="sequence: GGTATGTCCgccGGAtccACTgccgccGGAtccACTgccCTCCTGCTCGAACAGTTCCAG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          2505..2524
                     /label="oAFX452 2xGSGSG F2"
                     /note="sequence: ggcAGTggaTCCggcggcAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2505..2534
                     /label="2xGSGSG linker"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2507..2525)
                     /label="oAFX420 2xGSGSG R"
                     /note="sequence: ACTgccgccGGAtccACTg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2512..2534)
                     /label="oAFX453 2xGSGSG R2"
                     /note="sequence: gccGGAtccACTgccgccGGAtc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2513..2530
                     /label="oAFX421 2xGSGSG F"
                     /note="sequence: aTCCggcggcAGTggaTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2528..2546
                     /label="oAFX484 2xGSGSG eMagBF F"
                     /note="sequence: aTCCggcGGACATACCCTC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2535..2987
                     /label="eMagBF"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2535..2987
                     /label="eMagBF no start codon"
     misc_feature    2988..2996
                     /label="GSS"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2997..3038
                     /label="V5.a"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(2997..3069)
                     /label="M25_V5_NES"
                     /note="sequence: GCTCCTCCAGCTTCTTCTGCAGGCTACTgccCGTAGAATCGAGACCGAGGAGAGGGTTAGGGATAGGCTTACC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(3010..3069)
                     /label="oAFX191 N99.noMag.R2"
                     /note="sequence: GCTCCTCCAGCTTCTTCTGCAGGCTACTgccCGTAGAATCGAGACCGAGGAGAGGGTTAG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3039..3047
                     /label="GSS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3048..3077
                     /label="MEK1.NES"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(3048..3102)
                     /label="M26_NES_Xho1"
                     /note="sequence: agtgcggccgcgatatcctcgaTCACAGTTCCAGCTCCTCCAGCTTCTTCTGCAG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3081..3105
                     /label="XhoI 3' homology"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3081..3105)
                     /label="XhoI_Hom-R"
                     /note="sequence: aggagtgcggccgcgatatcctcga"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3126..3144)
                     /label="oAFX338 pCAGEN colony PCR R"
                     /note="sequence: cacaccagccaccaccttc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(3177..3196)
                     /label="Bglob-pA-R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(3218..3236)
                     /label="pCAGGs_R (QB4626)"
                     /note="sequence: GAAGTCAGATGCTCAAGGG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(3218..3236)
                     /label="GP0116 pCAGGs_R"
                     /note="sequence: GAAGTCAGATGCTCAAGGG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(3223..3243)
                     /label="pCX-cMyc-3 (QB4157)"
                     /note="sequence: TTAGCCAGAAGTCAGATGCTC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     polyA_site      3242..3297
                     /label="-beta-globin poly(A) signal"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(3296..3315)
                     /label="rbglobpA-R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(3296..3315)
                     /label="pYD5-R (QB1411)"
                     /note="sequence: CCCATATGTCCTTCCGAGTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(3658..3674)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(3658..3674)
                     /label="M13 Reverse"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3658..3674)
                     /label="QB0048 M13-Rev"
                     /note="sequence: CAGGAAACAGCTATGAC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(3671..3693)
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     protein_bind    3682..3698
                     /label="lac operator"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(3693..3709)
                     /label="QB0136 M13Reverse(-40)"
                     /note="sequence: GTTGTGTGGAATTGTGA"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        complement(3706..3736)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    3751..3772
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        3831..4026
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      3877..4012
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     3939..3958
                     /label="SV40pro-F"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     polyA_signal    4032..4166
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(4069..4088)
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     4123..4142
                     /label="EBV-rev"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(4234..4251)
                     /label="L4440"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      complement(4405..4993)
                     /label="ori"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(4485..4504)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(5164..6024)
                     /label="AmpR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     5787..5806
                     /label="Amp-R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          5954..5973
                     /label="oAFX429 Eu-Myc JAX-14378 R"
                     /note="sequence: TGAGCAAAAACAGGAAGGCA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        complement(6025..6129)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggcgcgcg ccrggggggg gggggggggg gggggggggg
      541 gggggggggg gggggggggg kgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
      721 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
      781 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
      841 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
      901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
      961 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
     1321 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1381 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     1441 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     1561 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     1621 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     1681 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt cctcgaATGA
     1741 AGGACAACAC TGTTCCGCTT AAGCTGATAG CCCTCCTGGC CAATGGAGAG TTCCACAGCG
     1801 GTGAGCAGCT CGGTGAGACT CTCGGGATGT CTCGCGCCGC TATTAACAAG CACATTCAGA
     1861 CGTTGCGCGA TTGGGGAGTC GACGTGTTCA CAGTACCTGG CAAAGGGTAC TCACTCCCTG
     1921 AGCCTATCCA GTTGCTGAAC GCAAAGCAGA TTCTGGGACA ACTGGACGGT GGGAGTGTTG
     1981 CCGTCCTCCC TGTGATCGAC TCCACGAACC AGTACTTGCT GGACCGCATC GGCGAGCTTA
     2041 AAAGCGGAGA TGCTTGTATC GCTGAATACC AGCAGGCCGG GCGCGGCAGA CGGGGCCGGA
     2101 AGTGGTTCTC TCCTTTTGGC GCAAATCTGT ACCTTTCTAT GTTCTGGAGG CTGGAACAGG
     2161 GACCAGCAGC CGCAATCGGC TTGTCCCTGG TCATTGGGAT TGTGATGGCC GAAGTTCTCA
     2221 GAAAGCTGGG AGCTGATAAG GTGAGGGTTA AATGGCCTAA CGATCTGTAT TTGCAGGACC
     2281 GGAAACTGGC CGGAATACTG GTGGAGCTGA CTGGCAAGAC TGGCGACGCC GCTCAGATAG
     2341 TCATCGGAGC CGGTATAAAT ATGGCCATGA GGCGCGTCGA GGAATCCGTG GTGAACCAAG
     2401 GCTGGATCAC CTTGCAAGAG GCCGGTATTA ACCTGGATAG AAACACCCTG GCCGCTATGC
     2461 TGATACGCGA ACTGCGCGCC GCCCTGGAAC TGTTCGAGCA GGAGggcAGT ggaTCCggcg
     2521 gcAGTggaTC CggcGGACAT ACCCTCTACG CGCCGGGGGG TTATGACATC ATGGGTTACC
     2581 TCAGACAGAT CAGAAACCGG CCGAACCCAC AAGTGGAGCT GGGACCCGTC GACCTCTCCT
     2641 GCGCCCTCGT GCTGTGTGAC CTTAAGCAGA AGGACACCCC TGTGGTGTAC GCCTCCGAAG
     2701 CATTCCTGGA GATGACCGGG TACAACAGAC ACGAAGTGCT GGGACGGAAC TGCCGCTTCC
     2761 TGCAATCCCC GGATGGAATG GTGAAGCCTA AGTCAACCCG CAAATACGTG GACTCCAACA
     2821 CTATCTTCAC CATGAAGAAG GCCATTGACC GCAATGCTGA GGTGCAAGTG GAAGTGGTGA
     2881 ACTTCAAGAA GAACGGACAG CGCTTCGTCA ACTTCCTGAC TATGATTCCC GTGCGGGACG
     2941 AAACCGGCGA ATACCGGTAC AGCATCGGGT TTCAGTGCGA GACTGAGgga TCCTCTGGTA
     3001 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGgg cAGTAGCCTG CAGAAGAAGC
     3061 TGGAGGAGCT GGAACTGTGA tcgaggatat cgcggccgca ctcctcaggt gcaggctgcc
     3121 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
     3181 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     3241 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     3301 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     3361 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
     3421 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     3481 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     3541 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     3601 gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc
     3661 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     3721 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     3781 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
     3841 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     3901 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     3961 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4021 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4081 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4141 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag
     4201 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     4261 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4321 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4381 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4441 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4501 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4561 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4621 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4681 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4741 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4801 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     4861 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4921 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4981 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5041 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5101 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     5161 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     5221 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     5281 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     5341 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     5401 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     5461 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     5521 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     5581 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     5641 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     5701 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     5761 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     5821 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     5881 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     5941 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     6001 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     6061 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     6121 ggttccgcgc acatttcccc gaaaagtgcc acctg
//