LOCUS       pCAGEN_NES_V5_eMagAF_C7 6446 bp ds-DNA     circular     27-FEB-2025
DEFINITION  .
KEYWORDS    "marker:SV40pA-R, rbglobpA-R, AmpR, Bglob-pA-R, Amp-R"
FEATURES             Location/Qualifiers
     source          1..6446
                     /label="source"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     enhancer        4..383
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(198..218)
                     /label="WQLI11 (QB4767) - bad for seq PX459"
                     /note="sequence: CCGTAAATACTCCACCCATTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(283..300)
                     /label="WQLI12 (QB4768)"
                     /note="sequence: CGGGCCATTTACCGTCAT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     intron          661..1677
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          1336..1359
                     /label="pCX-cMyc-5 (QB4156)"
                     /note="sequence: GCGAGCCGCAGCCATTGCCTTTTA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     1601..1623
                     /label="pCAGGS-5"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1601..1623
                     /label="oAFX083 pCAGGS-5"
                     /note="sequence: ggttcggcttctggcgtgtgacc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1632..1653
                     /label="QB1272 CAG-1F (RF GP0061 CAG-1F)"
                     /note="sequence: TAGAGCCTCTGCTAACCATGTT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1640..1659
                     /label="QB4180"
                     /note="sequence: CTGCTAACCATGTTCATGCC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1646..1663
                     /label="QB4625 (RF GP0115 pCAGGs_F)"
                     /note="sequence: ACCATGTTCATGCCTTCT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1684..1704
                     /label="oAFX339 pCAGEN colony PCR F"
                     /note="sequence: ggcaacgtgctggttattgtg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     1685..1704
                     /label="pCAG-F"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1712..1736
                     /label="XhoI.Gibson.5'.hom"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1712..1761
                     /label="oAFX171 XhoI5hom.ATG.NES.F"
                     /note="sequence: atcattttggcaaagaattcctcgaATGCTGCAGAAGAAGCTGGAGGAGC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1712..1769
                     /label="oAFX194 noMag.C100.F3"
                     /note="sequence: atcattttggcaaagaattcctcgaATGCTGCAGAAGAAGCTGGAGGAGCTGGAACTG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             1737..3371
                     /label="Translation 1737-3371"
                     /translation="MLQKKLEELELGSSGKPIPNPLLGLDSTGSSGHTLYAPGGYDIMGYLDQIANRPNPQVELGPVDLSCALILCDLKQKDTPIVYASEAFLEMTGYNRHEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTIFTIKKAIDRNAEVQVEVVNFKKNGQRFVNFLTIIPVRDETGEYRYSIGFQCETEGSGSGGSGSGGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEKGSSGSSGSSPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGRTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS*"
     misc_feature    1740..1769
                     /label="MEK1.NES"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1746..1805
                     /label="oAFX193 noMag.C100.F2"
                     /note="sequence: AAGAAGCTGGAGGAGCTGGAACTGggcTCTTCCGGTAAGCCTATCCCTAACCCTCTCCTC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1759..1818
                     /label="M12_NES_V5"
                     /note="sequence: AGCTGGAACTGggcTCTTCCGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1770..1778
                     /label="GSS"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1779..1820
                     /label="V5"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1821..1829
                     /label="GSS"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1830..2282
                     /label="eMagAF"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1830..2282
                     /label="eMagAF no start codon"
     primer          1833..1857
                     /label="M2_Mag_For"
                     /note="sequence: CACACTCTTTACGCCCCTGGAGGAT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(2096..2146)
                     /label="oAFX422 eMagAF R"
                     /note="sequence: CGGTCGATGGCCTTCTTGATGGTGAAGATCGTGTTCGAGTCCACATATTTG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2122..2168
                     /label="oAFX423 eMagAF F"
                     /note="sequence: TCACCATCAAGAAGGCCATCGACCGGAACGCCGAGGTCCAGGTGGAG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2283..2312
                     /label="2xGSGSG linker"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2285..2303)
                     /label="oAFX420 2xGSGSG R"
                     /note="sequence: ACTgccgccGGAtccACTg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          2291..2308
                     /label="oAFX421 2xGSGSG F"
                     /note="sequence: aTCCggcggcAGTggaTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             2313..3056
                     /label="C74"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2474..2494
                     /label="(52) BirA_cat_domain_internal_seq_F"
                     /note="sequence: TGGCGCAAATCTGTACCTTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2483..2513)
                     /label="M22_BirAN_Rev"
                     /note="sequence: TTCCAGCCTCCAGAACATAGAAAGGTACAGA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(2489..2516)
                     /label="S10_BirAN_140_Rev"
                     /note="sequence: CTGTTCCAGCCTCCAGAACATAGAAAGG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2755..2772
                     /label="(DS) BirA_Seq_F"
                     /note="sequence: AAGGCTGGATCACCTTGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2837..2861)
                     /label="S1_Split_Nterm_256"
                     /note="sequence: CTCCTGCTCGAACAGTTCCAGGGCG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(3026..3083)
                     /label="M31_biraC_Sec61_R"
                     /note="sequence: GGAAGAgccAGAGGAtccGCTACTgccCTTCTCTGCAGATCTAAGGGAGATTTCCCCG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             3057..3083
                     /label="GSS Linker"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     primer          3057..3100
                     /label="M30_biraC_Sec61"
                     /note="sequence: ggcAGTAGCggaTCCTCTggcTCTTCCcctggtccgacccccag"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          3057..3100
                     /label="oAFX015 GSSx3-SecB-F"
                     /note="sequence: ggcAGTAGCggaTCCTCTggcTCTTCCcctggtccgacccccag"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             3084..3368
                     /label="Sec61 beta human, aa 2-96"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(3341..3396)
                     /label="M32_Xho_Sec61R"
                     /note="sequence: aggagtgcggccgcgatatcctcgattacgaacgagtgtacttgccccaaatgtgc"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3372..3396
                     /label="XhoI.Gibson.3'.hom"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3417..3435)
                     /label="oAFX338 pCAGEN colony PCR R"
                     /note="sequence: cacaccagccaccaccttc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(3468..3487)
                     /label="Bglob-pA-R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(3509..3527)
                     /label="pCAGGs_R (QB4626)"
                     /note="sequence: GAAGTCAGATGCTCAAGGG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(3514..3534)
                     /label="pCX-cMyc-3 (QB4157)"
                     /note="sequence: TTAGCCAGAAGTCAGATGCTC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     polyA_site      3533..3588
                     /label="-beta-globin poly(A) signal"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(3587..3606)
                     /label="rbglobpA-R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(3587..3606)
                     /label="pYD5-R (QB1411)"
                     /note="sequence: CCCATATGTCCTTCCGAGTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(3949..3965)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(3949..3965)
                     /label="M13 Reverse"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3949..3965)
                     /label="QB0048 M13-Rev"
                     /note="sequence: CAGGAAACAGCTATGAC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(3962..3984)
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     protein_bind    3973..3989
                     /label="lac operator"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(3984..4000)
                     /label="QB0136 M13Reverse(-40)"
                     /note="sequence: GTTGTGTGGAATTGTGA"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        complement(3997..4027)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    4042..4063
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        4122..4317
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      4168..4303
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     4230..4249
                     /label="SV40pro-F"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     polyA_signal    4323..4457
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(4360..4379)
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     4414..4433
                     /label="EBV-rev"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(4525..4542)
                     /label="L4440"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      complement(4696..5284)
                     /label="ori"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(4776..4795)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(5455..6315)
                     /label="AmpR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     6078..6097
                     /label="Amp-R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(6316..6420)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggcgcgcg ccrggggggg gggggggggg gggggggggg
      541 gggggggggg gggggggggg kgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
      721 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
      781 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
      841 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
      901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
      961 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
     1321 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1381 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     1441 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     1561 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     1621 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     1681 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt cctcgaATGC
     1741 TGCAGAAGAA GCTGGAGGAG CTGGAACTGg gcTCTTCCGG TAAGCCTATC CCTAACCCTC
     1801 TCCTCGGTCT CGATTCTACG ggaTCCTCTG GACACACTCT TTACGCCCCT GGAGGATACG
     1861 ACATTATGGG ATATTTGGAT CAGATTGCGA ACCGCCCAAA CCCTCAGGTC GAACTGGGGC
     1921 CTGTGGACCT GTCATGTGCC CTGATCCTGT GCGATCTGAA GCAAAAGGAC ACTCCGATCG
     1981 TCTACGCCTC GGAAGCCTTC TTGGAGATGA CCGGATACAA CAGACATGAG GTGCTCGGCA
     2041 GGAACTGCAG ATTCCTGCAG TCCCCCGACG GGATGGTGAA ACCAAAGTCG ACTCGCAAAT
     2101 ATGTGGACTC GAACACGATC TTCACCATCA AGAAGGCCAT CGACCGGAAC GCCGAGGTCC
     2161 AGGTGGAGGT GGTCAACTTT AAGAAGAACG GCCAGCGGTT CGTGAACTTT CTGACCATCA
     2221 TTCCGGTCCG GGATGAAACC GGAGAGTACA GATACTCCAT CGGATTCCAG TGCGAAACCG
     2281 AAggcAGTgg aTCCggcggc AGTggaTCCg gcGGACAACT GGACGGTGGG AGTGTTGCCG
     2341 TCCTCCCTGT GATCGACTCC ACGAACCAGT ACTTGCTGGA CCGCATCGGC GAGCTTAAAA
     2401 GCGGAGATGC TTGTATCGCT GAATACCAGC AGGCCGGGCG CGGCAGACGG GGCCGGAAGT
     2461 GGTTCTCTCC TTTTGGCGCA AATCTGTACC TTTCTATGTT CTGGAGGCTG GAACAGGGAC
     2521 CAGCAGCCGC AATCGGCTTG TCCCTGGTCA TTGGGATTGT GATGGCCGAA GTTCTCAGAA
     2581 AGCTGGGAGC TGATAAGGTG AGGGTTAAAT GGCCTAACGA TCTGTATTTG CAGGACCGGA
     2641 AACTGGCCGG AATACTGGTG GAGCTGACTG GCAAGACTGG CGACGCCGCT CAGATAGTCA
     2701 TCGGAGCCGG TATAAATATG GCCATGAGGC GCGTCGAGGA ATCCGTGGTG AACCAAGGCT
     2761 GGATCACCTT GCAAGAGGCC GGTATTAACC TGGATAGAAA CACCCTGGCC GCTATGCTGA
     2821 TACGCGAACT GCGCGCCGCC CTGGAACTGT TCGAGCAGGA GGGACTCGCT CCATATCTGA
     2881 GTCGCTGGGA AAAATTGGAT AACTTCATAA ACCGACCCGT GAAGCTGATC ATCGGCGACA
     2941 AGGAAATTTT CGGAATCTCT CGCGGCATCG ATAAACAGGG GGCATTGCTG CTCGAGCAGG
     3001 ATGGGATTAT TAAGCCATGG ATGGGCGGGG AAATCTCCCT TAGATCTGCA GAGAAGggcA
     3061 GTAGCggaTC CTCTggcTCT TCCcctggtc cgacccccag tggcactaac gtgggatcct
     3121 cagggcgctc tcccagcaaa gcagtggccg cccgggcggc gggatccact gtccggcaga
     3181 ggaaaaatgc cagctgtggg acaaggagtg caggccgcac aacctcggca ggcaccgggg
     3241 ggatgtggcg attctacaca gaagattcac ctgggctcaa agttggccct gttccagtat
     3301 tggttatgag tcttctgttc atcgcttctg tatttatgtt gcacatttgg ggcaagtaca
     3361 ctcgttcgTA Atcgaggata tcgcggccgc actcctcagg tgcaggctgc ctatcagaag
     3421 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
     3481 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
     3541 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
     3601 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
     3661 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
     3721 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
     3781 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
     3841 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
     3901 ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt
     3961 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     4021 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     4081 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca
     4141 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4201 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     4261 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     4321 ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     4381 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4441 cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4501 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4561 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4621 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4681 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4741 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4801 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4861 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4921 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4981 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     5041 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     5101 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     5161 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5221 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5281 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5341 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5401 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5461 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5521 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     5581 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     5641 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     5701 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     5761 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     5821 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     5881 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     5941 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     6001 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     6061 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6121 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6181 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6241 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6301 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6361 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6421 cacatttccc cgaaaagtgc cacctg
//