LOCUS       Exported                2913 bp ds-DNA     circular SYN 07-NOV-2013
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     203..222
                     /label=T7
     misc_feature    311..316
                     /label=RBS
     misc_feature    325..348
                     /label=His6 tag (MAH6)
     misc_feature    349..360
                     /label=tag linker
     misc_feature    361..381
                     /label=TEV cleavage site (ENLYFQ//G)
     misc_feature    complement(704..726)
                     /label=T7R (T7 terminator primer)
     misc_feature    1011..1871
                     /label=AmpR(w/PstI)
     misc_feature    2042..2630
                     /label=pMB1 ori (from pBR322, without ROP gene)
     misc_feature    complement(2081..2188)
                     /label=RNAI
ORIGIN
        1 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
       61 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
      121 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
      181 caggatctcg atcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc
      241 tctagtgccg gctccggaga gctccaattg gaattcgcta gctctagaaa taattttgtt
      301 taactttaag aaggagatat aaatatggct caccatcatc atcatcattc ttctggttcc
      361 gaaaacctgt atttccaggg ctcccatatg gaagtaatgg aaggacccct caacctggct
      421 catcaacaga gcagacgagc agaccgttta ttagctgcag gcaaatacga agaggctatt
      481 tcttgtcaca aaaaggctgc agcatatctt tctgaagcca tgaagctgac acagtcagag
      541 caggctcatc tttcactgga attgcaaagg gatagccata tgaaacagct cctcctcatc
      601 caagagagat ggaaaagggc ccagtaggga tccagatcta agcttggtac cacgcgtgcg
      661 cgctgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
      721 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
      781 aggaggaact atagccggat aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
      841 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
      901 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      961 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     1021 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     1081 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     1141 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     1201 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     1261 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     1321 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     1381 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     1441 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     1501 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
     1561 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     1621 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     1681 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     1741 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     1801 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     1861 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     1921 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     1981 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2041 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2101 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2161 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     2221 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2281 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2341 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2401 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2461 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2521 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2581 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2641 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2701 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     2761 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     2821 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt gcactctcag
     2881 tacaatctgc tctgatgccg catagttaag cca
//
