LOCUS       Exported                6926 bp ds-DNA     circular SYN 05-OCT-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    18..381
                     /label=CMV enhencer
     misc_feature    382..663
                     /label=Chicken b-actin promoter
     misc_feature    664..1624
                     /label=intron chicken b-actin
     misc_feature    896..912
                     /label=yoo936
     misc_feature    1635..1679
                     /label=intron rabbit b-globin
     misc_feature    1723..1758
                     /label=5' infusion generic
     misc_feature    1759..1786
                     /label=HsUVRAG
     misc_feature    1789..3855
                     /label=HsUVRAG
                     /label=HsUVRAG(1)
     misc_feature    2412..2436
                     /label=YOo53 f
     misc_feature    2412..2436
                     /label=yoo53 f
     misc_feature    3856..3858
                     /label=stop
     misc_feature    3859..3888
                     /label=3' infusion generic
     misc_feature    3868..4394
                     /label=polyA-rabbit b-globin
     primer_bind     complement(4425..4445)
                     /label=M13-rev
     misc_binding    complement(4451..4473)
                     /label=LacO
     misc_feature    5161..5780
                     /label=pBR322origin
     misc_feature    5618..5725
                     /label=RNAI
     misc_feature    complement(5935..6597)
                     /label=AmpR
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgctcgatc
     1741 gacggtatcg atgccaccat gagcgcctcc gcgtcggtcg ggggccaggt cccccagcca
     1801 cccccgggcc cggccgctgc tctgcctccc ggttctgccg cgcgggccct gcatgtggag
     1861 ctgccgtctc agcagcggcg tcttcgacat cttcggaaca ttgctgcccg gaacattgtt
     1921 aatagaaatg gccatcagct ccttgatacc tactttacac ttcacttgtg tagtactgaa
     1981 aagatatata aagaatttta tagaagtgaa gtgattaaga attccttgaa tcccacgtgg
     2041 cgaagtctcg attttggaat tatgccagac cgtcttgata catctgtgtc ttgtttcgtg
     2101 gtgaagatat ggggtggaaa ggagaacatc taccagctgt tgattgaatg gaaagtctgt
     2161 ttggatgggc tgaaatactt gggtcagcag attcatgccc gaaaccaaaa tgaaataatt
     2221 tttgggctga atgatggata ctatggtgct ccatttgaac ataagggtta ttcaaatgct
     2281 cagaagacta ttcttctgca ggtggatcag aactgtgttc gcaattctta cgatgtcttc
     2341 tctttgctac ggcttcatag agcccagtgt gcaattaaac agactcaggt aactgttcag
     2401 aaaattggaa aggaaattga agaaaaacta agactcacat ctacaagcaa tgaactgaaa
     2461 aaaaaaagtg aatgcctgca gttaaaaatt ttggtgcttc agaatgaact ggaacggcag
     2521 aagaaagctt tgggacggga ggtggcatta ctgcataagc aacaaattgc attacaagac
     2581 aaaggaagtg cattttcagc tgagcacctc aaacttcaac tccagaagga atccctaaat
     2641 gagctgagga aggagtgcac tgcaaaaaga gaactcttct tgaagactaa tgctcagttg
     2701 acaattcgtt gcaggcagtt actctctgag ctttcctaca tttaccctat tgatttgaat
     2761 gaacataagg attactttgt atgcggtgtc aagttgccta attctgagga cttccaagca
     2821 aaagatgatg gaagcattgc tgttgccctt ggttatactg cacatctggt ctccatgatt
     2881 tcctttttcc tacaagtgcc cctcagatat cctataattc ataaggggtc tagatcaaca
     2941 atcaaagaca atatcaatga caaactgacg gaaaaggaga gagagtttcc actgtatcca
     3001 aaaggagggg agaagttgca gtttgattat ggtgtctatc ttctgaacaa aaatatagca
     3061 cagctaagat atcaacatgg actagggact ccagacttgc ggcaaaccct tcccaacctg
     3121 aaaaacttca tggagcatgg actaatggtc aggtgtgaca gacatcacac ctccagtgca
     3181 atccctgttc ctaagagaca aagctccata tttgggggtg cagatgtagg cttctctggg
     3241 gggatccctt caccagacaa aggacatcga aaacgggcca gctctgagaa tgagagactt
     3301 cagtacaaaa cccctcctcc cagttacaac tcagcattag cccagcctgt gaccaccgtc
     3361 ccctccatgg gagagaccga gagaaagata acatctctat cctcctcctt ggatacctcc
     3421 ttggacttct ccaaagaaaa caagaaaaaa ggagaggatc tagttggcag cttaaacgga
     3481 ggccacgcga atgtgcaccc tagccaagaa caaggagaag ccctctccgg gcaccgggcc
     3541 acagtcaatg gcactctcct acccagcgag caggccgggt ccgccagtgt ccagcttcca
     3601 ggcgagttcc acccagtctc agaagctgag ctctgctgta ctgtggagca agcagaagaa
     3661 atcatcgggc tggaagccac aggtttcgcc tcaggtgatc agctagaagc atttaactgc
     3721 atcccagtgg acagtgctgt ggcagtagag tgtgacgaac aagttctggg agaatttgaa
     3781 gagttctccc gaaggatcta tgcactgaat gaaaacgtat ccagcttccg ccggccgcgc
     3841 aggagttccg ataagtaact cgaggaattc actcctcagg tgcaggctgc ctatcagaag
     3901 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
     3961 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
     4021 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
     4081 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
     4141 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
     4201 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
     4261 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
     4321 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
     4381 ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt
     4441 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     4501 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     4561 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca
     4621 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4681 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     4741 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     4801 ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     4861 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4921 cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4981 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     5041 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     5101 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     5161 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     5221 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     5281 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     5341 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
     5401 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     5461 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     5521 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     5581 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     5641 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5701 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5761 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5821 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5881 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5941 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     6001 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     6061 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     6121 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     6181 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     6241 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     6301 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     6361 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     6421 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     6481 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     6541 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6601 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6661 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6721 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6781 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6841 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6901 cacatttccc cgaaaagtgc cacctg
//
