LOCUS       Exported                9476 bp ds-DNA     circular SYN 11-SEP-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9476
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    18..381
                     /label=CMV enhencer
     misc_feature    382..663
                     /label=Chicken b-actin promoter
     misc_feature    664..1624
                     /label=intron chicken b-actin
     misc_feature    1635..1679
                     /label=intron rabbit b-globin
     misc_feature    1723..1758
                     /label=5' infusion generic
     misc_feature    1759..5832
                     /label=hsVPS15 fl (minus stop)
     misc_feature    1759..1761
                     /label=ATG start
     misc_feature    2210..2229
                     /label=Synthesised primer 1
     misc_feature    2210..2229
                     /label=OP0635
     misc_feature    2943..2961
                     /label=LZo14
     misc_feature    3328..3356
                     /label=sam098(182-512)
     misc_feature    3328..3356
                     /label=SAM097F
     misc_feature    3328..3356
                     /label=sam097(542-842)
     misc_feature    3328..3356
                     /label=SAM098R
     misc_feature    4546..4564
                     /label=yoO933 for
     misc_feature    4653..4674
                     /label=(LZ011)R
     misc_feature    4653..4674
                     /label=LZo15 R
     misc_feature    4996..5004
                     /label=PKI, HDX protected
     misc_feature    5233..5260
                     /label=sam0100(869-1148)
     misc_feature    5233..5260
                     /label=SAM0100R
     misc_feature    5233..5260
                     /label=SAM099F
     misc_feature    5233..5252
                     /label=yoO934 for
     misc_feature    5833..5976
                     /label=linker
     misc_feature    5854..5874
                     /label=TEV
     misc_feature    5896..5916
                     /label=TEV
                     /label=TEV(1)
     misc_feature    5977..5994
                     /label=GAGA linker
     misc_feature    5995..6396
                     /label=zz
     misc_feature    6400..6408
                     /label=ADH1 teminator
     misc_feature    6409..6438
                     /label=infusion generic
     misc_feature    6418..6944
                     /label=polyA-rabbit b-globin
     misc_feature    6945..6962
                     /label=yoo930r
     primer_bind     complement(6975..6995)
                     /label=M13-rev
     misc_binding    complement(7001..7023)
                     /label=LacO
     misc_feature    7711..8330
                     /label=pBR322origin
     misc_feature    8168..8275
                     /label=RNAI
     misc_feature    complement(8485..9147)
                     /label=AmpR
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgctcgatc
     1741 gacggtatcg atgccaccat gggaaatcag cttgctggca ttgctccctc ccagatcctt
     1801 tctgtagaga gttatttttc agatattcat gactttgaat atgataaaag cctggggagt
     1861 actcggtttt ttaaagttgc tcgagccaag caccgagaag gcctggtcgt tgtgaaggtt
     1921 tttgcaattc aggatcccac attgccttta accagctata aacaagagct ggaggaactg
     1981 aaaatcaggc ttaattctgc acagaattgt ctacctttcc agaaagcatc agaaaaagca
     2041 tctgagaaag cagctatgct ctttaggcag tatgtgcgag acaatctcta tgatcgcatc
     2101 agtacccgtc cattcttgaa taacattgag aagcgctgga ttgctttcca gatcctgaca
     2161 gctgtggacc aagcacacaa atctggagtt cgtcatgggg acatcaagac tgagaatgtg
     2221 atggtcacca gttggaattg ggttcttcta actgattttg ccagttttaa gcccacttat
     2281 cttccagaag acaacccggc agatttcaat tatttctttg acacatcacg gaggagaact
     2341 tgctatattg ctcctgaacg ttttgttgat ggtgggatgt ttgccactga gttagaatat
     2401 atgagagatc cttcaactcc gcttgtagac ttaaatagca atcagagaac aagaggagag
     2461 ttgaagagag caatggacat cttttcagca ggttgtgtga tagctgagct ttttacagaa
     2521 ggtgtaccat tatttgatct ctctcaactt ttggcttata gaaatggaca ttttttccct
     2581 gaacaagtgc taaataaaat tgaagatcac agtatcagag aattggtaac tcagatgatt
     2641 caccgtgagc cagataaacg tttagaggca gaagattact taaaacagca gcgtggcaat
     2701 gcctttcctg aaatatttta cacttttctt cagccctaca tggcccagtt tgccaaggaa
     2761 acgtttcttt ctgcagatga gcgtattctg gttatacgga aggatttggg caacattatt
     2821 cacaatctct gtggacatga tctgccagaa aaagccgaag gagagcctaa ggaaaatggg
     2881 ctggttatct tggtatctgt tataacatcc tgcctacaga cccttaaata ctgtgattcc
     2941 aaactagctg ctttggaact gattcttcat ttggctccaa gattaagtgt tgaaatcctt
     3001 ttggatcgta ttactccata tcttttgcat ttcagcaatg actctgttcc tagggtgagg
     3061 gctgaagcct tgaggacgtt gaccaaagtt cttgctctcg tcaaagaggt tcctcgtaat
     3121 gatatcaata tttatccgga atacattctg ccaggcatag cccacttagc ccaagatgat
     3181 gctactatcg ttagactagc ctatgctgaa aacatagctc tgctggcaga aacagctctg
     3241 agattcctgg aattagtaca gttaaaaaat cttaatatgg aaaatgaccc caataatgaa
     3301 gaaatagatg aggttacaca tccaaatgga aattatgaca cagagctgca agccttacat
     3361 gaaatggtcc agcagaaagt tgttactttg ctaagtgacc ctgaaaatat tgtaaaacaa
     3421 accttgatgg aaaatggaat aacacggctg tgtgtattct ttggacgtca gaaagccaac
     3481 gatgttttgt tgtcccacat gattactttc ctaaatgata agaatgattg gcatctacgt
     3541 ggagcatttt ttgatagtat agttggtgtt gctgcctatg ttggctggca aagctcctca
     3601 attctcaagc ctctgctgca acaaggtctt agtgatgctg aggaatttgt cattgtgaaa
     3661 gctctttatg cccttacttg tatgtgccag ttaggactgc tacaaaaacc ccatgtttac
     3721 gaatttgcca gtgatattgc ccccttcctg tgtcatccca atttatggat acgttatggt
     3781 gccgtgggat ttatcacagt ggtagctcgt caaataagta cagctgatgt ctactgtaaa
     3841 ctgatgcctt atcttgaccc atatattacc caaccaataa tacagattga aagaaaactt
     3901 gttctgctca gtgttttaaa ggaaccagta agtcgttcta tatttgatta tgctttgagg
     3961 tctaaagata ttactagctt gttcagacat cttcacatgc gtcagaagaa acgaaatggt
     4021 tctcttcccg actgccctcc gccagaggat cctgccatag cacagcttct gaagaagttg
     4081 ctctcacagg gaatgacaga ggaagaggaa gacaaacttc tggcactgaa agacttcatg
     4141 atgaaatcta ataaagcaaa ggccaatata gtggaccaga gccatcttca tgatagtagt
     4201 cagaaaggtg taattgactt ggcagcttta ggcataactg ggagacaagt tgatcttgtt
     4261 aaaaccaaac aagaaccaga tgacaaacgg gccagaaaac atgtaaaaca agactcaaat
     4321 gtaaatgaag aatggaaaag catgtttggg tcactggacc caccaaacat gccacaggcc
     4381 ctacctaaag ggagtgatca ggaggtgatt cagactggga aacctcctcg ttccgagtcc
     4441 tctgctggca tttgtgtccc tttgtcaact tcttcacagg ttccagaagt gacaactgtc
     4501 caaaataaaa aaccagtaat accggtttta agtagtacaa tcttaccatc cacctatcag
     4561 attcgaatta caacttgtaa aactgaactt cagcaactca tccagcaaaa gcgggagcag
     4621 tgcaatgctg agagaatagc taagcagatg atggaaaatg ctgaatggga gagtaaacca
     4681 ccaccacctg gatggcgtcc taaagggctg ttagttgccc atcttcatga gcataaatct
     4741 gctgtgaatc gaattagagt ctctgatgaa cactcacttt ttgcaacatg ttcaaatgat
     4801 ggcacagtga aaatctggaa cagtcaaaag atggagggga agaccaccac taccagatct
     4861 attcttacat acagccgaat tggaggacga gtcaagacgc tcacattctg ccaaggctcc
     4921 cactatttag ccatagcatc tgataatggt gctgtccagc ttcttggaat tgaggcttct
     4981 aagctgccca agtctcctaa aatccatcct ctacaaagca gaattctaga tcagaaggag
     5041 gacggttgtg ttgtggatat gcatcacttc aactctggag cacagtctgt tcttgcctat
     5101 gccactgtga atggctctct ggttggctgg gaccttaggt cttcaagcaa tgcgtggact
     5161 ttaaagcatg atttaaagtc gggcctcatc acttcctttg ctgtggacat ccaccaatgc
     5221 tggctctgca ttggtacaag cagtggcacc atggcttgtt gggacatgag gttccagttg
     5281 ccaatttcaa gtcactgtca tccttccagg gctcgaatca gacgcctctc aatgcaccct
     5341 ctgtatcagt cctgggtgat tgcagctgtt cagggcaaca acgaagtgtc catgtgggac
     5401 atggagactg gtgacagaag atttactctc tgggccagca gtgcaccacc actttctgaa
     5461 ttacagcctt ctcctcatag cgtccatggt atctactgta gtcctgcaga tggaaatcct
     5521 atcctactaa cagctggctc agatatgaaa ataaggtttt gggacttggc ttacccagaa
     5581 aggtcctatg ttgttgcagg aagtactagt tccccatctg tgtcctacta caggaaaata
     5641 attgaaggca ctgaagttgt ccaggaaatt cagaataagc agaaagtagg accaagtgat
     5701 gacacccctc gaaggggccc agagtccctg cccgtgggac atcatgacat catcactgat
     5761 gtcgccacat tccagaccac acagggcttc atcgtaactg cttctagaga tgggattgtg
     5821 aaggtgtgga aatctagacc aactactgcc tctgaaaatt tgtactttca aggcgagttg
     5881 aagaccactg cttctgaaaa cttgtacttc caaggtgagt tactcgaggg tgaattgaag
     5941 actgctgctt tggctcaaca cgacgaagct agatccggag caggggcggg tgctagcgag
     6001 aacctgtact tccagggcgc ccacgatgaa gccgtagaca acaaattcaa caaagaacaa
     6061 caaaacgcgt tctatgagat cttacattta cctaacttaa acgaagaaca acgaaacgcc
     6121 ttcatccaaa gtttaaaaga tgacccaagc caaagcgcta accttttagc agaagctaaa
     6181 aagctaaatg atgctcaggc gccgaaagta gacaacaaat tcaacaaaga acaacaaaac
     6241 gcgttctatg agatcttaca tttacctaac ttaaacgaag aacaacgaaa cgccttcatc
     6301 caaagtttaa aagatgaccc aagccaaagc gctaaccttt tagcagaagc taaaaagcta
     6361 aatgatgctc aggcgccgaa agtagacgcg aattcgtgag ctcgcgaact cgaggaattc
     6421 actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct
     6481 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc
     6541 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
     6601 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
     6661 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa
     6721 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc
     6781 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt
     6841 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc
     6901 tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc
     6961 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7021 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7081 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7141 ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     7201 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7261 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     7321 ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt
     7381 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7441 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg
     7501 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7561 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7621 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7681 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7741 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7801 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7861 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7921 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7981 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8041 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8101 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8161 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8221 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8281 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8341 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8401 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8461 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8521 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8581 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8641 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8701 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8761 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8821 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8881 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8941 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9001 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9061 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9121 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9181 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9241 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9301 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9361 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9421 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctg
//
