LOCUS       Exported                9280 bp ds-DNA     circular SYN 21-NOV-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9280
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..725
                     /label=CMV promoter
     misc_feature    571..577
                     /label=TATA
     misc_feature    587..626
                     /label=x2 TetO2
     misc_feature    join(734..756,2111..2135)
                     /label=MCS
     misc_feature    join(757..2063,2065..2075,2077..2090,2092..2109)
                     /label=Beclin FL
     misc_feature    2153..2377
                     /label=PolA
     misc_feature    2378..2735
                     /label=CMV enhencer
     misc_feature    2736..3017
                     /label=Chicken b-actin promoter
     misc_feature    3018..3978
                     /label=intron chicken b-actin
     misc_feature    3250..3266
                     /label=yoo936
     misc_feature    3989..4033
                     /label=intron rabbit b-globin
     misc_feature    4077..4112
                     /label=5' infusion generic
     misc_feature    4113..4140
                     /label=HsUVRAG
     misc_feature    4143..6209
                     /label=HsUVRAG
                     /label=HsUVRAG(1)
     misc_feature    4766..4790
                     /label=YOo53 f
     misc_feature    4766..4790
                     /label=yoo53 f
     misc_feature    6210..6212
                     /label=stop
     misc_feature    6213..6242
                     /label=3' infusion generic
     misc_feature    6222..6748
                     /label=polyA-rabbit b-globin
     primer_bind     complement(6779..6799)
                     /label=M13-rev
     misc_binding    complement(6805..6827)
                     /label=LacO
     misc_feature    7515..8134
                     /label=pBR322origin
     misc_feature    7972..8079
                     /label=RNAI
     misc_feature    complement(8289..8951)
                     /label=AmpR
ORIGIN
        1 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
       61 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      121 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      181 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      241 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      301 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      361 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      421 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      481 tggaaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      541 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
      601 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      661 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      721 ctccggactc tagcgtttaa acttaagctt ggtaccatgg aagggtctaa gacgtccaac
      781 aacagcacca tgcaggtgag cttcgtgtgc cagcgctgca gccagcccct gaaactggac
      841 acgagtttca agatcctgga ccgtgtcacc atccaggaac tcacagctcc attacttacc
      901 acagcccagg cgaaaccagg agagacccag gaggaagaga ctaactcagg agaggagcca
      961 tttattgaaa ctcctcgcca ggatggtgtc tctcgcagat tcatcccccc agccaggatg
     1021 atgtccacag aaagtgccaa cagcttcact ctgattgggg aggcatctga tggcggcacc
     1081 atggagaacc tcagccgaag actgaaggtc actggggacc tttttgacat catgtcgggc
     1141 cagacagatg tggatcaccc actctgtgag gaatgcacag atactctttt agaccagctg
     1201 gacactcagc tcaacgtcac tgaaaatgag tgtcagaact acaaacgctg tttggagatc
     1261 ttagagcaaa tgaatgagga tgacagtgaa cagttacaga tggagctaaa ggagctggca
     1321 ctagaggagg agaggctgat ccaggagctg gaagacgtgg aaaagaaccg caagatagtg
     1381 gcagaaaatc tcgagaaggt ccaggctgag gctgagagac tggatcagga ggaagctcag
     1441 tatcagagag aatacagtga atttaaacga cagcagctgg agctggatga tgagctgaag
     1501 agtgttgaaa accagatgcg ttatgcccag acgcagctgg ataagctgaa gaaaaccaac
     1561 gtctttaatg caaccttcca catctggcac agtggacagt ttggcacaat caataacttc
     1621 aggctgggtc gcctgcccag tgttcccgtg gaatggaatg agattaatgc tgcttggggc
     1681 cagactgtgt tgctgctcca tgctctggcc aataagatgg gtctgaaatt tcagagatac
     1741 cgacttgttc cttacggaaa ccattcatat ctggagtctc tgacagacaa atctaaggag
     1801 ctgccgttat actgttctgg ggggttgcgg tttttctggg acaacaagtt tgaccatgca
     1861 atggtggctt tcctggactg tgtgcagcag ttcaaagaag aggttgagaa aggcgagaca
     1921 cgtttttgtc ttccctacag gatggatgtg gagaaaggca agattgaaga cacaggaggc
     1981 agtggcggct cctattccat caaaacccag tttaactctg aggagcagtg gacaaaagct
     2041 ctcaagttca tgctgacgaa tctgaagtgg ggtctggctt gggtgtcctc tcaattttat
     2101 aacaaatgag tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
     2161 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     2221 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     2281 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     2341 aatagcaggc atgctgggga tgcggtgggc tctatggtat taatagtaat caattacggg
     2401 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     2461 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     2521 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     2581 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     2641 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     2701 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
     2761 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
     2821 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
     2881 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
     2941 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
     3001 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgcgcc
     3061 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
     3121 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt
     3181 ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc
     3241 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc
     3301 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag
     3361 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc
     3421 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg
     3481 caaccccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     3541 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     3601 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg
     3661 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
     3721 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
     3781 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
     3841 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
     3901 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
     3961 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt
     4021 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
     4081 agaattgctc gatcgacggt atcgatgcca ccatgagcgc ctccgcgtcg gtcgggggcc
     4141 aggtccccca gccacccccg ggcccggccg ctgctctgcc tcccggttct gccgcgcggg
     4201 ccctgcatgt ggagctgccg tctcagcagc ggcgtcttcg acatcttcgg aacattgctg
     4261 cccggaacat tgttaataga aatggccatc agctccttga tacctacttt acacttcact
     4321 tgtgtagtac tgaaaagata tataaagaat tttatagaag tgaagtgatt aagaattcct
     4381 tgaatcccac gtggcgaagt ctcgattttg gaattatgcc agaccgtctt gatacatctg
     4441 tgtcttgttt cgtggtgaag atatggggtg gaaaggagaa catctaccag ctgttgattg
     4501 aatggaaagt ctgtttggat gggctgaaat acttgggtca gcagattcat gcccgaaacc
     4561 aaaatgaaat aatttttggg ctgaatgatg gatactatgg tgctccattt gaacataagg
     4621 gttattcaaa tgctcagaag actattcttc tgcaggtgga tcagaactgt gttcgcaatt
     4681 cttacgatgt cttctctttg ctacggcttc atagagccca gtgtgcaatt aaacagactc
     4741 aggtaactgt tcagaaaatt ggaaaggaaa ttgaagaaaa actaagactc acatctacaa
     4801 gcaatgaact gaaaaaaaaa agtgaatgcc tgcagttaaa aattttggtg cttcagaatg
     4861 aactggaacg gcagaagaaa gctttgggac gggaggtggc attactgcat aagcaacaaa
     4921 ttgcattaca agacaaagga agtgcatttt cagctgagca cctcaaactt caactccaga
     4981 aggaatccct aaatgagctg aggaaggagt gcactgcaaa aagagaactc ttcttgaaga
     5041 ctaatgctca gttgacaatt cgttgcaggc agttactctc tgagctttcc tacatttacc
     5101 ctattgattt gaatgaacat aaggattact ttgtatgcgg tgtcaagttg cctaattctg
     5161 aggacttcca agcaaaagat gatggaagca ttgctgttgc ccttggttat actgcacatc
     5221 tggtctccat gatttccttt ttcctacaag tgcccctcag atatcctata attcataagg
     5281 ggtctagatc aacaatcaaa gacaatatca atgacaaact gacggaaaag gagagagagt
     5341 ttccactgta tccaaaagga ggggagaagt tgcagtttga ttatggtgtc tatcttctga
     5401 acaaaaatat agcacagcta agatatcaac atggactagg gactccagac ttgcggcaaa
     5461 cccttcccaa cctgaaaaac ttcatggagc atggactaat ggtcaggtgt gacagacatc
     5521 acacctccag tgcaatccct gttcctaaga gacaaagctc catatttggg ggtgcagatg
     5581 taggcttctc tggggggatc ccttcaccag acaaaggaca tcgaaaacgg gccagctctg
     5641 agaatgagag acttcagtac aaaacccctc ctcccagtta caactcagca ttagcccagc
     5701 ctgtgaccac cgtcccctcc atgggagaga ccgagagaaa gataacatct ctatcctcct
     5761 ccttggatac ctccttggac ttctccaaag aaaacaagaa aaaaggagag gatctagttg
     5821 gcagcttaaa cggaggccac gcgaatgtgc accctagcca agaacaagga gaagccctct
     5881 ccgggcaccg ggccacagtc aatggcactc tcctacccag cgagcaggcc gggtccgcca
     5941 gtgtccagct tccaggcgag ttccacccag tctcagaagc tgagctctgc tgtactgtgg
     6001 agcaagcaga agaaatcatc gggctggaag ccacaggttt cgcctcaggt gatcagctag
     6061 aagcatttaa ctgcatccca gtggacagtg ctgtggcagt agagtgtgac gaacaagttc
     6121 tgggagaatt tgaagagttc tcccgaagga tctatgcact gaatgaaaac gtatccagct
     6181 tccgccggcc gcgcaggagt tccgataagt aactcgagga attcactcct caggtgcagg
     6241 ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag
     6301 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
     6361 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
     6421 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
     6481 agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag
     6541 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga
     6601 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa
     6661 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca
     6721 tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca
     6781 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6841 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6901 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc
     6961 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     7021 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7081 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7141 gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7201 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7261 actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg
     7321 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     7381 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7441 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7501 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7561 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7621 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7681 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     7741 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7801 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7861 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7921 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     7981 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8041 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8101 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8161 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8221 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8281 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8341 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     8401 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8461 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8521 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8581 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8641 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8701 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8761 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8821 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8881 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8941 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9001 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9061 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9121 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9181 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9241 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
//
