LOCUS       Exported                8662 bp ds-DNA     circular SYN 30-MAY-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..725
                     /label=CMV promoter
     misc_feature    571..577
                     /label=TATA
     misc_feature    587..626
                     /label=x2 TetO2
     misc_feature    join(734..756,2111..2135)
                     /label=MCS
     misc_feature    join(757..2063,2065..2075,2077..2090,2092..2109)
                     /label=Beclin FL
     misc_feature    2153..2377
                     /label=PolA
     misc_feature    2378..2735
                     /label=CMV enhencer
     misc_feature    2736..3017
                     /label=Chicken b-actin promoter
     misc_feature    3018..3978
                     /label=intron chicken b-actin
     misc_feature    3989..4033
                     /label=intron rabbit b-globin
     misc_feature    4077..4112
                     /label=5' infusion generic
     misc_feature    4113..4115
                     /label=start
     misc_feature    4119..5591
                     /label=ATG14L N256S
     misc_feature    4882
                     /label=mutation1
     misc_feature    5595..5624
                     /label=3' infusion generi
     misc_feature    5604..6130
                     /label=polyA-rabbit b-globin
     primer_bind     complement(6161..6181)
                     /label=M13-rev
     misc_binding    complement(6187..6209)
                     /label=LacO
     misc_feature    6897..7516
                     /label=pBR322origin
     misc_feature    7354..7461
                     /label=RNAI
     misc_feature    complement(7671..8333)
                     /label=AmpR
ORIGIN
        1 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
       61 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      121 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      181 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      241 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      301 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      361 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      421 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      481 tggaaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      541 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
      601 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      661 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      721 ctccggactc tagcgtttaa acttaagctt ggtaccatgg aagggtctaa gacgtccaac
      781 aacagcacca tgcaggtgag cttcgtgtgc cagcgctgca gccagcccct gaaactggac
      841 acgagtttca agatcctgga ccgtgtcacc atccaggaac tcacagctcc attacttacc
      901 acagcccagg cgaaaccagg agagacccag gaggaagaga ctaactcagg agaggagcca
      961 tttattgaaa ctcctcgcca ggatggtgtc tctcgcagat tcatcccccc agccaggatg
     1021 atgtccacag aaagtgccaa cagcttcact ctgattgggg aggcatctga tggcggcacc
     1081 atggagaacc tcagccgaag actgaaggtc actggggacc tttttgacat catgtcgggc
     1141 cagacagatg tggatcaccc actctgtgag gaatgcacag atactctttt agaccagctg
     1201 gacactcagc tcaacgtcac tgaaaatgag tgtcagaact acaaacgctg tttggagatc
     1261 ttagagcaaa tgaatgagga tgacagtgaa cagttacaga tggagctaaa ggagctggca
     1321 ctagaggagg agaggctgat ccaggagctg gaagacgtgg aaaagaaccg caagatagtg
     1381 gcagaaaatc tcgagaaggt ccaggctgag gctgagagac tggatcagga ggaagctcag
     1441 tatcagagag aatacagtga atttaaacga cagcagctgg agctggatga tgagctgaag
     1501 agtgttgaaa accagatgcg ttatgcccag acgcagctgg ataagctgaa gaaaaccaac
     1561 gtctttaatg caaccttcca catctggcac agtggacagt ttggcacaat caataacttc
     1621 aggctgggtc gcctgcccag tgttcccgtg gaatggaatg agattaatgc tgcttggggc
     1681 cagactgtgt tgctgctcca tgctctggcc aataagatgg gtctgaaatt tcagagatac
     1741 cgacttgttc cttacggaaa ccattcatat ctggagtctc tgacagacaa atctaaggag
     1801 ctgccgttat actgttctgg ggggttgcgg tttttctggg acaacaagtt tgaccatgca
     1861 atggtggctt tcctggactg tgtgcagcag ttcaaagaag aggttgagaa aggcgagaca
     1921 cgtttttgtc ttccctacag gatggatgtg gagaaaggca agattgaaga cacaggaggc
     1981 agtggcggct cctattccat caaaacccag tttaactctg aggagcagtg gacaaaagct
     2041 ctcaagttca tgctgacgaa tctgaagtgg ggtctggctt gggtgtcctc tcaattttat
     2101 aacaaatgag tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
     2161 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     2221 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     2281 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     2341 aatagcaggc atgctgggga tgcggtgggc tctatggtat taatagtaat caattacggg
     2401 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     2461 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     2521 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     2581 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     2641 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     2701 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
     2761 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
     2821 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
     2881 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
     2941 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
     3001 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgcgcc
     3061 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
     3121 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt
     3181 ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc
     3241 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc
     3301 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag
     3361 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc
     3421 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg
     3481 caaccccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     3541 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     3601 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg
     3661 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
     3721 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
     3781 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
     3841 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
     3901 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
     3961 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt
     4021 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
     4081 agaattgctc gatcgacggt atcgatgcca ccatgacggc gtctcccagt gggaagggag
     4141 cccgggcgct ggaggctcct ggctgcgggc cccggccgct cgcccgggac ctggtggact
     4201 ccgtggacga tgcggagggg ctgtacgtgg ctgtggagcg ctgcccgctg tgcaacacta
     4261 cccgccggcg gctgacctgc gccaaatgcg ttcagagcgg cgatttcgtc tacttcgacg
     4321 gccgcgaccg ggagaggttt atcgacaaga aggaaaggtt aagccgactt aagagcaagc
     4381 aagaagaatt tcagaaagaa gtgttaaaag ctatggaagg aaaatggata acagatcagt
     4441 tgagatggaa aataatgtcc tgcaagatga ggattgaaca gttaaaacaa acaatatgta
     4501 aaggaaatga agaaatggag aaaaattctg aaggccttct caaaaccaag gaaaagaatc
     4561 agaagcttta cagtcgagca caacggcacc aagagaaaaa ggagaagatt cagaggcata
     4621 atcgcaaact tggtgacctg gtagaaaaaa agaccattga cttaagaagt cattatgagc
     4681 gtctggcaaa tcttcgacga tcccatatat tagagctcac ctctgtcatt tttccaatcg
     4741 aggaagtaaa gacgggtgtg agagaccccg cagatgtgtc ttcagagagt gacagtgcca
     4801 tgacctccag cactgtgagc aagcttgctg aagcccggag gacaacttac ctctcaggac
     4861 gatgggtctg tgacgatcac agcggagaca ccagcattag cattacaggg ccttggatta
     4921 gcctccctaa caatggggac tactctgcct actacagctg ggtggaggag aagaaaacaa
     4981 cccaggggcc tgacatggag cagagtaacc ctgcctacac catcagtgct gcgctgtgct
     5041 atgcaactca gctggtcaac attctgtctc atatacttga tgtaaatctt cccaaaaagc
     5101 tctgcaacag tgaattttgt ggcgaaaatc taagcaagca gaaatttact cgagcagtga
     5161 agaaactgaa tgcaaatatt ctttaccttt gtttttctca gcatgtaaat ttagatcaat
     5221 tacaaccact gcataccctc aggaatctaa tgtacctggt cagtccaagc tctgaacacc
     5281 taggcaggtc agggcccttt gaagtacgag cagaccttga ggagtccatg gaatttgtgg
     5341 atcccggagt tgctggagaa tcagatgaga gcggagatga gcgcgtcagc gatgaagaaa
     5401 ccgacctggg cacagactgg gagaacttgc ctagtccccg gttttgtgat atcccttccc
     5461 agtctgtgga agtctcccag agtcagagca cccaggcgtc cccacccatc gcgagcagca
     5521 gtgcaggtgg gatgatctcc tctgcagcag cctcggtgac ctcctggttt aaagcttaca
     5581 ctggacaccg ttaactcgag gaattcactc ctcaggtgca ggctgcctat cagaaggtgg
     5641 tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca
     5701 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
     5761 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     5821 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
     5881 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
     5941 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
     6001 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
     6061 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
     6121 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct
     6181 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6241 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6301 gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca
     6361 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     6421 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     6481 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa
     6541 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6601 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6661 tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     6721 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6781 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6841 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6901 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6961 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7021 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7081 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     7141 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7201 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7261 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7321 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     7381 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7441 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7501 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7561 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7621 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7681 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7741 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7801 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7861 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7921 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7981 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8041 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8101 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8161 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8221 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8281 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8341 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8401 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8461 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8521 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8581 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8641 tttccccgaa aagtgccacc tg
//
