LOCUS       Exported                7681 bp ds-DNA     circular SYN 01-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7681
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    232..958
                     /label=CMV promoter
     misc_feature    804..810
                     /label=TATA
     misc_feature    820..859
                     /label=x2 TetO2
     misc_feature    join(967..989,3660..3680)
                     /label=MCS
     misc_feature    990..992
                     /label=ATG start
     misc_feature    join(996..1583,1596..3650)
                     /label=HsVps34-full length (1-887)
     misc_feature    1113..1139
                     /label=PMLKFSGLY
     misc_feature    1584..1595
                     /label=REIE to AAAA
     misc_feature    1896..1915
                     /label=yoo937
     misc_feature    2010..2034
                     /label=LZo108
     misc_feature    2561..2584
                     /label=LZo109
     misc_feature    3698..3922
                     /label=PolA
     rep_origin      3985..4291
                     /label=F1 ori
     primer_bind     complement(5523..5543)
                     /label=M13-rev
     misc_binding    complement(5549..5571)
                     /label=LacO
     CDS             complement(6688..7347)
                     /label=AmpR
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggaacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtacca tgggggaagc agagaagttt cactacatct
     1021 atagttgtga cctggatatc aacgtccagc ttaagatagg aagcttggaa gggaagagag
     1081 aacaaaagag ttataaagct gtcctggaag acccaatgtt gaagttctca ggactatatc
     1141 aagagacatg ctctgatctt tatgttactt gtcaagtttt tgcagaaggg aagcctttgg
     1201 ccttgccagt gagaacatcc tacaaagcat ttagtacaag atggaactgg aatgaatggc
     1261 tgaaactacc agtaaaatac cctgacctgc ccaggaatgc ccaagtggcc ctcaccatct
     1321 gggatgtgta tggtcccgga aaagcagtgc ctgtaggagg aacaacggtt tcgctctttg
     1381 gaaaatacgg catgtttcgc caagggatgc atgacttgaa agtctggcct aatgtagaag
     1441 cagatggatc agaacccaca aaaactcctg gcagaacaag tagcactctc tcagaagatc
     1501 agatgagccg tcttgccaag ctcaccaaag ctcatcgaca aggacacatg gtgaaagtag
     1561 attggctgga tagattgaca tttgctgcag ctgcaatgat aaatgagagt gaaaaacgaa
     1621 gttctaattt catgtacctg atggttgaat ttcgatgtgt caagtgtgat gataaggaat
     1681 atggtattgt ttattatgaa aaggacggtg atgaatcatc tccaatttta acaagttttg
     1741 aattagtgaa agttcctgac ccccagatgt ctatggagaa tttagttgag agcaaacacc
     1801 acaagcttgc ccggagttta agaagtggac cttctgacca cgatctgaaa cccaatgctg
     1861 ccacgagaga tcagttaaat attattgtga gttatccacc aaccaagcaa cttacctatg
     1921 aagaacaaga tcttgtttgg aagtttagat attatcttac gaatcaagaa aaagccttga
     1981 caaaattctt gaaatgtgtt aattgggatc tacctcaaga ggccaaacag gccttggaac
     2041 ttctgggaaa atggaagccg atggatgtag aggactcctt ggagctgtta tcctctcatt
     2101 acaccaaccc aactgtgagg cgttatgctg ttgcccggtt gcgacaggcc gatgatgagg
     2161 atttgttgat gtacctatta caattggtcc aggctctcaa atatgaaaat tttgatgata
     2221 taaagaatgg attggaacct accaagaagg atagtcagag ttcagtgtca gaaaatgtgt
     2281 caaattctgg aataaattct gcagaaatag atagctccca aattataacc agcccccttc
     2341 cttcagtctc ttcacctcct cctgcatcaa aaacaaaaga agttccagat ggcgaaaatc
     2401 tggaacaaga tctctgtacc ttcttgatat cgagagcctg caaaaactca acactggcta
     2461 attatttata ctggtatgtg atagtggaat gtgaagatca agatactcag cagagagatc
     2521 caaagaccca tgagatgtac ttgaacgtaa tgagaagatt cagccaagca ttgttgaagg
     2581 gtgataagtc tgtcagagtt atgcgttctt tgctggctgc acaacagaca tttgtagatc
     2641 ggttggtgca tctaatgaag gcagtacaac gcgaaagtgg aaatcgtaag aaaaagaatg
     2701 agagactaca ggcattgctt ggagataatg aaaagatgaa tttgtcagat gtggaactta
     2761 tcccgttgcc tttagaaccc caagtgaaaa ttagaggaat aattccggaa acagctacac
     2821 tgtttaaaag tgcccttatg cctgcacagt tgttttttaa gacggaagat ggaggcaaat
     2881 atccagttat atttaagcat ggagatgatt tacgtcaaga tcaacttatt cttcaaatca
     2941 tttcactcat ggacaagctg ttacggaaag aaaatctgga cttgaaattg acaccttata
     3001 aggtgttagc caccagtaca aaacatggct tcatgcagtt tatccagtca gttcctgtgg
     3061 ctgaagttct tgatacagag ggaagcattc agaacttttt tagaaaatat gcaccaagtg
     3121 agaatgggcc aaatgggatt agtgctgagg tcatggacac ttacgttaaa agctgtgctg
     3181 gatattgcgt gatcacctat atacttggag ttggagacag gcacctggat aaccttttgc
     3241 taacaaaaac aggcaaactc ttccacatag actttggata tattctgggt cgtgatccaa
     3301 agcctcttcc tccaccaatg aagctgaata aagaaatggt agaaggaatg gggggcacac
     3361 agagtgagca gtaccaagag ttccgtaaac agtgttacac ggctttcctc cacctgcgaa
     3421 ggtattctaa tctgattttg aacttgtttt ccttgatggt tgatgcaaac attccagata
     3481 ttgcacttga accagataaa actgtgaaaa aggttcagga taaattccgc ttagacctgt
     3541 cggatgaaga ggctgtgcat tacatgcaga gtctgattga tgagagtgtc catgctcttt
     3601 ttgctgcagt ggtggaacag attcacaagt ttgcccagta ctggcgcaaa tgagtcgagg
     3661 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     3721 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3781 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3841 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     3901 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     3961 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4021 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4081 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4141 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4201 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4261 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4321 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4381 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     4441 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     4501 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4561 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4621 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     4681 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     4741 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
     4801 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
     4861 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     4921 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     4981 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     5041 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     5101 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     5161 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     5221 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     5281 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     5341 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     5401 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     5461 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     5521 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     5581 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     5641 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     5701 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     5761 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     5821 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     5881 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     5941 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6001 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6061 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6121 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6181 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6241 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6301 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6361 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6421 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa
     6481 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6541 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6601 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6661 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     6721 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     6781 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     6841 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     6901 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     6961 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7021 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7081 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7141 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7201 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7261 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7321 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7381 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7441 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7501 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     7561 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7621 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     7681 c
//
