LOCUS       Exported                9087 bp ds-DNA     circular SYN 07-JUN-2012
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9087
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    232..958
                     /label=CMV promoter
     misc_feature    804..810
                     /label=TATA
     misc_feature    820..859
                     /label=x2 TetO2
     misc_feature    967..983
                     /label=MCS
     misc_feature    984..5057
                     /label=hsVPS15 fl (minus stop)
     misc_feature    984..986
                     /label=ATG start
     misc_feature    1435..1454
                     /label=Synthesised primer 1
     misc_feature    1435..1454
                     /label=OP0635
     misc_feature    2168..2186
                     /label=LZo14
     misc_feature    2553..2581
                     /label=SAM097F
     misc_feature    2553..2581
                     /label=sam097(542-842)
     misc_feature    2553..2581
                     /label=SAM098R
     misc_feature    2553..2581
                     /label=sam098(182-512)
     misc_feature    3878..3899
                     /label=(LZ011)R
     misc_feature    3878..3899
                     /label=LZo15 R
     misc_feature    4458..4485
                     /label=sam0100(869-1148)
     misc_feature    4458..4485
                     /label=SAM0100R
     misc_feature    4458..4485
                     /label=SAM099F
     misc_feature    5061..5086
                     /label=MCS
                     /label=MCS(1)
     misc_feature    5104..5328
                     /label=PolA
     rep_origin      5391..5697
                     /label=F1 ori
     primer_bind     complement(6929..6949)
                     /label=M13-rev
     misc_binding    complement(6955..6977)
                     /label=LacO
     CDS             complement(8094..8753)
                     /label=AmpR
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggaacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttatgggaa atcagcttgc tggcattgct ccctcccaga
     1021 tcctttctgt agagagttat ttttcagata ttcatgactt tgaatatgat aaaagcctgg
     1081 ggagtactcg gttttttaaa gttgctcgag ccaagcaccg agaaggcctg gtcgttgtga
     1141 aggtttttgc aattcaggat cccacattgc ctttaaccag ctataaacaa gagctggagg
     1201 aactgaaaat caggcttaat tctgcacaga attgtctacc tttccagaaa gcatcagaaa
     1261 aagcatctga gaaagcagct atgctcttta ggcagtatgt gcgagacaat ctctatgatc
     1321 gcatcagtac ccgtccattc ttgaataaca ttgagaagcg ctggattgct ttccagatcc
     1381 tgacagctgt ggaccaagca cacaaatctg gagttcgtca tggggacatc aagactgaga
     1441 atgtgatggt caccagttgg aattgggttc ttctaactga ttttgccagt tttaagccca
     1501 cttatcttcc agaagacaac ccggcagatt tcaattattt ctttgacaca tcacggagga
     1561 gaacttgcta tattgctcct gaacgttttg ttgatggtgg gatgtttgcc actgagttag
     1621 aatatatgag agatccttca actccgcttg tagacttaaa tagcaatcag agaacaagag
     1681 gagagttgaa gagagcaatg gacatctttt cagcaggttg tgtgatagct gagcttttta
     1741 cagaaggtgt accattattt gatctctctc aacttttggc ttatagaaat ggacattttt
     1801 tccctgaaca agtgctaaat aaaattgaag atcacagtat cagagaattg gtaactcaga
     1861 tgattcaccg tgagccagat aaacgtttag aggcagaaga ttacttaaaa cagcagcgtg
     1921 gcaatgcctt tcctgaaata ttttacactt ttcttcagcc ctacatggcc cagtttgcca
     1981 aggaaacgtt tctttctgca gatgagcgta ttctggttat acggaaggat ttgggcaaca
     2041 ttattcacaa tctctgtgga catgatctgc cagaaaaagc cgaaggagag cctaaggaaa
     2101 atgggctggt tatcttggta tctgttataa catcctgcct acagaccctt aaatactgtg
     2161 attccaaact agctgctttg gaactgattc ttcatttggc tccaagatta agtgttgaaa
     2221 tccttttgga tcgtattact ccatatcttt tgcatttcag caatgactct gttcctaggg
     2281 tgagggctga agccttgagg acgttgacca aagttcttgc tctcgtcaaa gaggttcctc
     2341 gtaatgatat caatatttat ccggaataca ttctgccagg catagcccac ttagcccaag
     2401 atgatgctac tatcgttaga ctagcctatg ctgaaaacat agctctgctg gcagaaacag
     2461 ctctgagatt cctggaatta gtacagttaa aaaatcttaa tatggaaaat gaccccaata
     2521 atgaagaaat agatgaggtt acacatccaa atggaaatta tgacacagag ctgcaagcct
     2581 tacatgaaat ggtccagcag aaagttgtta ctttgctaag tgaccctgaa aatattgtaa
     2641 aacaaacctt gatggaaaat ggaataacac ggctgtgtgt attctttgga cgtcagaaag
     2701 ccaacgatgt tttgttgtcc cacatgatta ctttcctaaa tgataagaat gattggcatc
     2761 tacgtggagc attttttgat agtatagttg gtgttgctgc ctatgttggc tggcaaagct
     2821 cctcaattct caagcctctg ctgcaacaag gtcttagtga tgctgaggaa tttgtcattg
     2881 tgaaagctct ttatgccctt acttgtatgt gccagttagg actgctacaa aaaccccatg
     2941 tttacgaatt tgccagtgat attgccccct tcctgtgtca tcccaattta tggatacgtt
     3001 atggtgccgt gggatttatc acagtggtag ctcgtcaaat aagtacagct gatgtctact
     3061 gtaaactgat gccttatctt gacccatata ttacccaacc aataatacag attgaaagaa
     3121 aacttgttct gctcagtgtt ttaaaggaac cagtaagtcg ttctatattt gattatgctt
     3181 tgaggtctaa agatattact agcttgttca gacatcttca catgcgtcag aagaaacgaa
     3241 atggttctct tcccgactgc cctccgccag aggatcctgc catagcacag cttctgaaga
     3301 agttgctctc acagggaatg acagaggaag aggaagacaa acttctggca ctgaaagact
     3361 tcatgatgaa atctaataaa gcaaaggcca atatagtgga ccagagccat cttcatgata
     3421 gtagtcagaa aggtgtaatt gacttggcag ctttaggcat aactgggaga caagttgatc
     3481 ttgttaaaac caaacaagaa ccagatgaca aacgggccag aaaacatgta aaacaagact
     3541 caaatgtaaa tgaagaatgg aaaagcatgt ttgggtcact ggacccacca aacatgccac
     3601 aggccctacc taaagggagt gatcaggagg tgattcagac tgggaaacct cctcgttccg
     3661 agtcctctgc tggcatttgt gtccctttgt caacttcttc acaggttcca gaagtgacaa
     3721 ctgtccaaaa taaaaaacca gtaataccgg ttttaagtag tacaatctta ccatccacct
     3781 atcagattcg aattacaact tgtaaaactg aacttcagca actcatccag caaaagcggg
     3841 agcagtgcaa tgctgagaga atagctaagc agatgatgga aaatgctgaa tgggagagta
     3901 aaccaccacc acctggatgg cgtcctaaag ggctgttagt tgcccatctt catgagcata
     3961 aatctgctgt gaatcgaatt agagtctctg atgaacactc actttttgca acatgttcaa
     4021 atgatggcac agtgaaaatc tggaacagtc aaaagatgga ggggaagacc accactacca
     4081 gatctattct tacatacagc cgaattggag gacgagtcaa gacgctcaca ttctgccaag
     4141 gctcccacta tttagccata gcatctgata atggtgctgt ccagcttctt ggaattgagg
     4201 cttctaagct gcccaagtct cctaaaatcc atcctctaca aagcagaatt ctagatcaga
     4261 aggaggacgg ttgtgttgtg gatatgcatc acttcaactc tggagcacag tctgttcttg
     4321 cctatgccac tgtgaatggc tctctggttg gctgggacct taggtcttca agcaatgcgt
     4381 ggactttaaa gcatgattta aagtcgggcc tcatcacttc ctttgctgtg gacatccacc
     4441 aatgctggct ctgcattggt acaagcagtg gcaccatggc ttgttgggac atgaggttcc
     4501 agttgccaat ttcaagtcac tgtcatcctt ccagggctcg aatcagacgc ctctcaatgc
     4561 accctctgta tcagtcctgg gtgattgcag ctgttcaggg caacaacgaa gtgtccatgt
     4621 gggacatgga gactggtgac agaagattta ctctctgggc cagcagtgca ccaccacttt
     4681 ctgaattaca gccttctcct catagcgtcc atggtatcta ctgtagtcct gcagatggaa
     4741 atcctatcct actaacagct ggctcagata tgaaaataag gttttgggac ttggcttacc
     4801 cagaaaggtc ctatgttgtt gcaggaagta ctagttcccc atctgtgtcc tactacagga
     4861 aaataattga aggcactgaa gttgtccagg aaattcagaa taagcagaaa gtaggaccaa
     4921 gtgatgacac ccctcgaagg ggcccagagt ccctgcccgt gggacatcat gacatcatca
     4981 ctgatgtcgc cacattccag accacacagg gcttcatcgt aactgcttct agagatggga
     5041 ttgtgaaggt gtggaaataa ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct
     5101 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     5161 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     5221 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     5281 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     5341 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     5401 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     5461 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     5521 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     5581 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     5641 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5701 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     5761 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     5821 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5881 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5941 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     6001 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6061 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     6121 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     6181 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     6241 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     6301 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     6361 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     6421 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     6481 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     6541 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     6601 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
     6661 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     6721 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     6781 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6841 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     6901 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6961 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     7021 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7081 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7141 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7201 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7261 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7321 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7381 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7441 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7501 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7561 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7621 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7681 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7741 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     7801 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7861 gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7921 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7981 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8041 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8101 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8161 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8221 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8281 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8341 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8401 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8461 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8521 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8581 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8641 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8701 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8761 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8821 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8881 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8941 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9001 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9061 tttccccgaa aagtgccacc tgacgtc
//
