LOCUS       Exported                3765 bp ds-DNA     linear   UNA 28-NOV-2022
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    205..1065
                     /locus_tag="AmpR(w/PstI)"
                     /label=AmpR(w/PstI)
     misc_feature    1236..1824
                     /locus_tag="pMB1 ori (from pBR322, without ROP gene)"
                     /label=pMB1 ori (from pBR322, without ROP gene)
     misc_feature    complement(1275..1382)
                     /locus_tag="RNAI"
                     /label=RNAI
     primer_bind     2310..2329
                     /locus_tag="T7"
                     /label=T7
     misc_feature    2407..2415
                     /locus_tag="TRANSLATIONAL ENHANCER"
                     /label=TRANSLATIONAL ENHANCER
     misc_feature    2435..3094
                     /locus_tag="GST tag"
                     /label=GST tag
     misc_feature    3095..3106
                     /locus_tag="tag linker"
                     /label=tag linker
     misc_feature    3107..3127
                     /locus_tag="TEV cleavage site (ENLYFQ//G)"
                     /label=TEV cleavage site (ENLYFQ//G)
     misc_feature    join(3134..3136,3140..3586)
                     /locus_tag="1-149-PX_p40phox"
                     /label=1-149-PX_p40phox
     misc_feature    3137..3139
                     /locus_tag="Cys insertion"
                     /label=Cys insertion
     misc_feature    3386..3388
                     /locus_tag="C85S"
                     /label=C85S
     misc_feature    complement(3663..3685)
                     /locus_tag="T7R (T7 terminator primer)"
                     /label=T7R (T7 terminator primer)
ORIGIN
        1 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
       61 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      121 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      181 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      241 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      301 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      361 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      421 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
      481 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      541 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
      601 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
      661 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
      721 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
      781 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
      841 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
      901 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
      961 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     1021 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     1081 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1141 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     1201 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1261 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1321 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1381 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1441 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1501 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1561 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1621 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1681 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1741 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1801 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1861 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1921 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     1981 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     2041 tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     2101 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     2161 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     2221 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     2281 cgcgaggcag gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg
     2341 gtttccctct agtgccggct ccggagagct ccaattggaa ttcgctagct ctagaaataa
     2401 ttttgtttaa ctttaagaag gagatataca taatatgtcc cctatactag gttattggaa
     2461 aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga
     2521 agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg
     2581 tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat
     2641 ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg
     2701 tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat
     2761 tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat
     2821 gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac
     2881 ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg
     2941 cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat
     3001 tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct ggcaagccac
     3061 gtttggtggt ggcgaccatc ctccaaaatc ggattcttct ggttccgaaa acctgtattt
     3121 ccagggctcc catatgtgcg ctgtggccca gcagctgcgg gccgagagtg actttgaaca
     3181 gcttccggat gatgttgcca tctcggccaa cattgctgac atcgaggaga agagaggctt
     3241 caccagccac tttgttttcg tcatcgaggt gaagacaaaa ggaggatcca agtacctcat
     3301 ctaccgccgc taccgccagt tccatgcttt gcagagcaag ctggaggagc gcttcgggcc
     3361 agacagcaag agcagtgccc tggccagtac cctgcccaca ctcccagcca aagtctacgt
     3421 gggtgtgaaa caggagatcg ccgagatgcg gatacctgcc ctcaacgcct acatgaagag
     3481 cctgctcagc ctgccggtct gggtgctgat ggatgaggac gtccggatct tcttttacca
     3541 gtcgccctat gactcagagc aggtgcccca ggccatccgc cggtgaggat ccagatctaa
     3601 gcttggtacc acgcgtgcgc gctgatccgg ctgctaacaa agcccgaaag gaagctgagt
     3661 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
     3721 tgaggggttt tttgctgaaa ggaggaacta tagccggata attct
//
