LOCUS       Exported                6179 bp ds-DNA     circular SYN 24-SEP-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    18..381
                     /label=CMV enhencer
     misc_feature    382..663
                     /label=Chicken b-actin promoter
     misc_feature    664..1624
                     /label=intron chicken b-actin
     misc_feature    1635..1679
                     /label=intron rabbit b-globin
     misc_feature    1723..1758
                     /label=5' infusion generic
     misc_feature    join(1759..3065,3067..3077,3079..3092,3094..3111)
                     /label=Beclin FL
     misc_feature    3112..3141
                     /label=3' infusion generi
     misc_feature    3121..3647
                     /label=polyA-rabbit b-globin
     primer_bind     complement(3678..3698)
                     /label=M13-rev
     misc_binding    complement(3704..3726)
                     /label=LacO
     misc_feature    4414..5033
                     /label=pBR322origin
     misc_feature    4871..4978
                     /label=RNAI
     misc_feature    complement(5188..5850)
                     /label=AmpR
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgctcgatc
     1741 gacggtatcg atgccaccat ggaagggtct aagacgtcca acaacagcac catgcaggtg
     1801 agcttcgtgt gccagcgctg cagccagccc ctgaaactgg acacgagttt caagatcctg
     1861 gaccgtgtca ccatccagga actcacagct ccattactta ccacagccca ggcgaaacca
     1921 ggagagaccc aggaggaaga gactaactca ggagaggagc catttattga aactcctcgc
     1981 caggatggtg tctctcgcag attcatcccc ccagccagga tgatgtccac agaaagtgcc
     2041 aacagcttca ctctgattgg ggaggcatct gatggcggca ccatggagaa cctcagccga
     2101 agactgaagg tcactgggga cctttttgac atcatgtcgg gccagacaga tgtggatcac
     2161 ccactctgtg aggaatgcac agatactctt ttagaccagc tggacactca gctcaacgtc
     2221 actgaaaatg agtgtcagaa ctacaaacgc tgtttggaga tcttagagca aatgaatgag
     2281 gatgacagtg aacagttaca gatggagcta aaggagctgg cactagagga ggagaggctg
     2341 atccaggagc tggaagacgt ggaaaagaac cgcaagatag tggcagaaaa tctcgagaag
     2401 gtccaggctg aggctgagag actggatcag gaggaagctc agtatcagag agaatacagt
     2461 gaatttaaac gacagcagct ggagctggat gatgagctga agagtgttga aaaccagatg
     2521 cgttatgccc agacgcagct ggataagctg aagaaaacca acgtctttaa tgcaaccttc
     2581 cacatctggc acagtggaca gtttggcaca atcaataact tcaggctggg tcgcctgccc
     2641 agtgttcccg tggaatggaa tgagattaat gctgcttggg gccagactgt gttgctgctc
     2701 catgctctgg ccaataagat gggtctgaaa tttcagagat accgacttgt tccttacgga
     2761 aaccattcat atctggagtc tctgacagac aaatctaagg agctgccgtt atactgttct
     2821 ggggggttgc ggtttttctg ggacaacaag tttgaccatg caatggtggc tttcctggac
     2881 tgtgtgcagc agttcaaaga agaggttgag aaaggcgaga cacgtttttg tcttccctac
     2941 aggatggatg tggagaaagg caagattgaa gacacaggag gcagtggcgg ctcctattcc
     3001 atcaaaaccc agtttaactc tgaggagcag tggacaaaag ctctcaagtt catgctgacg
     3061 aatctgaagt ggggtctggc ttgggtgtcc tctcaatttt ataacaaatg actcgaggaa
     3121 ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg
     3181 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag
     3241 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
     3301 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
     3361 cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac
     3421 aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta
     3481 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt
     3541 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc
     3601 tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag
     3661 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     3721 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     3781 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     3841 gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     3901 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     3961 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     4021 ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg
     4081 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4141 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta
     4201 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     4261 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     4321 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     4381 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4441 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4501 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4561 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4621 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4681 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4741 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4801 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4861 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4921 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4981 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5041 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5101 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     5161 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     5221 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     5281 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     5341 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     5401 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     5461 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     5521 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     5581 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     5641 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     5701 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     5761 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     5821 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     5881 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     5941 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     6001 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     6061 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     6121 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctg
//
