LOCUS       Exported                6461 bp ds-DNA     circular SYN 05-DEC-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    18..381
                     /label=CMV enhencer
     misc_feature    382..663
                     /label=Chicken b-actin promoter
     misc_feature    664..1624
                     /label=intron chicken b-actin
     misc_feature    896..912
                     /label=yoo936
     misc_feature    1635..1679
                     /label=intron rabbit b-globin
     misc_feature    1723..1758
                     /label=5' infusion generic
     misc_feature    1759..1786
                     /label=HsUVRAG
     misc_feature    1789..3150
                     /label=HsUVRAG
                     /label=HsUVRAG(1)
     misc_feature    2412..2436
                     /label=YOo53 f
     misc_feature    2412..2436
                     /label=yoo53 f
     misc_feature    3151..3390
                     /label=ATG14L N256S
     misc_feature    3394..3423
                     /label=3' infusion generic
     misc_feature    3403..3929
                     /label=polyA-rabbit b-globin
     primer_bind     complement(3960..3980)
                     /label=M13-rev
     misc_binding    complement(3986..4008)
                     /label=LacO
     misc_feature    4696..5315
                     /label=pBR322origin
     misc_feature    5153..5260
                     /label=RNAI
     misc_feature    complement(5470..6132)
                     /label=AmpR
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgctcgatc
     1741 gacggtatcg atgccaccat gagcgcctcc gcgtcggtcg ggggccaggt cccccagcca
     1801 cccccgggcc cggccgctgc tctgcctccc ggttctgccg cgcgggccct gcatgtggag
     1861 ctgccgtctc agcagcggcg tcttcgacat cttcggaaca ttgctgcccg gaacattgtt
     1921 aatagaaatg gccatcagct ccttgatacc tactttacac ttcacttgtg tagtactgaa
     1981 aagatatata aagaatttta tagaagtgaa gtgattaaga attccttgaa tcccacgtgg
     2041 cgaagtctcg attttggaat tatgccagac cgtcttgata catctgtgtc ttgtttcgtg
     2101 gtgaagatat ggggtggaaa ggagaacatc taccagctgt tgattgaatg gaaagtctgt
     2161 ttggatgggc tgaaatactt gggtcagcag attcatgccc gaaaccaaaa tgaaataatt
     2221 tttgggctga atgatggata ctatggtgct ccatttgaac ataagggtta ttcaaatgct
     2281 cagaagacta ttcttctgca ggtggatcag aactgtgttc gcaattctta cgatgtcttc
     2341 tctttgctac ggcttcatag agcccagtgt gcaattaaac agactcaggt aactgttcag
     2401 aaaattggaa aggaaattga agaaaaacta agactcacat ctacaagcaa tgaactgaaa
     2461 aaaaaaagtg aatgcctgca gttaaaaatt ttggtgcttc agaatgaact ggaacggcag
     2521 aagaaagctt tgggacggga ggtggcatta ctgcataagc aacaaattgc attacaagac
     2581 aaaggaagtg cattttcagc tgagcacctc aaacttcaac tccagaagga atccctaaat
     2641 gagctgagga aggagtgcac tgcaaaaaga gaactcttct tgaagactaa tgctcagttg
     2701 acaattcgtt gcaggcagtt actctctgag ctttcctaca tttaccctat tgatttgaat
     2761 gaacataagg attactttgt atgcggtgtc aagttgccta attctgagga cttccaagca
     2821 aaagatgatg gaagcattgc tgttgccctt ggttatactg cacatctggt ctccatgatt
     2881 tcctttttcc tacaagtgcc cctcagatat cctataattc ataaggggtc tagatcaaca
     2941 atcaaagaca atatcaatga caaactgacg gaaaaggaga gagagtttcc actgtatcca
     3001 aaaggagggg agaagttgca gtttgattat ggtgtctatc ttctgaacaa aaatatagca
     3061 cagctaagat atcaacatgg actagggact ccagacttgc ggcaaaccct tcccaacctg
     3121 aaaaacttca tggagcatgg actaatggtc gctggagaat cagatgagag cggagatgag
     3181 cgcgtcagcg atgaagaaac cgacctgggc acagactggg agaacttgcc tagtccccgg
     3241 ttttgtgata tcccttccca gtctgtggaa gtctcccaga gtcagagcac ccaggcgtcc
     3301 ccacccatcg cgagcagcag tgcaggtggg atgatctcct ctgcagcagc ctcggtgacc
     3361 tcctggttta aagcttacac tggacaccgt taactcgagg aattcactcc tcaggtgcag
     3421 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga
     3481 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
     3541 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
     3601 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
     3661 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
     3721 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
     3781 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
     3841 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
     3901 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc
     3961 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     4021 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     4081 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat
     4141 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     4201 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4261 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4321 ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4381 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4441 aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc
     4501 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     4561 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     4621 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     4681 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     4741 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     4801 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     4861 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag
     4921 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4981 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     5041 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     5101 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     5161 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     5221 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     5281 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     5341 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     5401 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     5461 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     5521 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     5581 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     5641 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     5701 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     5761 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     5821 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     5881 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     5941 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     6001 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     6061 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     6121 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     6181 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     6241 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     6301 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     6361 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     6421 aataggggtt ccgcgcacat ttccccgaaa agtgccacct g
//
