LOCUS       Exported               11280 bp ds-DNA     circular SYN 22-JUN-2012
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11280
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=M13 origin
     rep_origin      complement(1483..1789)
                     /direction=LEFT
                     /label=F1 ori
     CDS             complement(1811..1879)
                     /label=LacZ alpha
     primer_bind     1950..1967
                     /label=M13-fwd
     primer_bind     1976..1996
                     /label=T7
     misc_feature    join(2010..2040,2042..2506)
                     /label=GALGAPDH
     misc_feature    complement(2513..2911)
                     /label=ScVPS15 coding minus stop
                     /label=ScVPS15 coding minus stop(2)
     misc_feature    complement(2912)
                     /label=T134A
     misc_feature    complement(2913..5061)
                     /label=ScVPS15 coding minus stop
                     /label=ScVPS15 coding minus stop(1)
     misc_feature    complement(5062..5064)
                     /label=I851R
     misc_feature    complement(5065..6874)
                     /label=ScVPS15 coding minus stop
     misc_feature    6881..7024
                     /label=linker
     misc_feature    6902..6922
                     /label=TEV
     misc_feature    6944..6964
                     /label=TEV
                     /label=TEV(1)
     misc_feature    7025..7042
                     /label=GAGA linker
     misc_feature    7043..7444
                     /label=zz
     misc_feature    7448..7641
                     /label=ADH1 teminator
     primer_bind     complement(7676..7695)
                     /label=T3
     primer_bind     complement(7713..7733)
                     /label=M13-rev
     misc_binding    complement(7739..7761)
                     /label=LacO
     rep_origin      complement(8101..8783)
                     /direction=LEFT
                     /label=ColE1 origin
     misc_feature    8163..8181
                     /label=YO032
     CDS             complement(8881..9540)
                     /label=AmpR
     misc_recomb     complement(10833..10866)
                     /label=FRT
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     1981 acgactcact atagggcgaa ttgggtaccg ctatgacatg attacgaatt ggcgcgccgg
     2041 gccgcggatc tttgcaaaga tccaaaaaaa gcatgcgtac ggattagaag ccgccgagcg
     2101 ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt
     2161 cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac aataaagatt ctacaatact
     2221 agcttttatg gttatgaaga ggaaaattgg cagtaacctg gccccacaaa cctcaaatga
     2281 acgaatcaaa ttaacaacca gatatctcga ctgaaaaaaa aggtttaaac cagttccctg
     2341 aaattattcc cctacttgac taataagtat ataaagacgg taggtattga ttgtaattct
     2401 gtaaatctat ttcttaaact tcttgaattc tacttttata gttagtcttt tttttagttt
     2461 taaaacacca agaacttagt ttcgaataaa cacacataaa taaaccctcg agatgggggc
     2521 acaattatca ctagtggtcc aagcatcacc ttccatagcc attttttcat atatcgatgt
     2581 cttagaggaa gtacactacg tttcacagtt aaactcatca agattcttaa aaacatgcaa
     2641 ggcactggac cctaacggcg aaattgttat caaagtgttt attaaaccaa aagaccaata
     2701 tagcttacga ccttttctcc aacgtataag ggctcaatcg tttaagttgg gacaactacc
     2761 gcacgtttta aactacagta aattgatcga gacaaataga gccggctaca tgatacggca
     2821 gcacttaaaa aataatttat atgacagatt gagtttgaga ccttacttac aagacattga
     2881 actgaaattc attgctttcc agttgttaaa tgcattaaag gacattcata atctgaatat
     2941 tgtccatggt gatataaaga cagaaaatat cctagtaaca agttggaatt ggtgtatatt
     3001 gacagatttt gctgcattta tcaaacccgt atatttgcct gaagataatc caggtgaatt
     3061 tttattctac ttcgacacct cgaagagaag aacctgttat ctagccccgg agaggtttaa
     3121 ctctaaactt taccaagatg gaaaatctaa caatggtagg ctaactaaag aaatggacat
     3181 atttagtctt ggatgtgtta ttgcagaaat atttgctgaa ggaagaccca tcttcaactt
     3241 atcacagcta ttcaaatata aaagtaattc atatgacgta aacagggaat ttctcatgga
     3301 ggaaatgaat tctaccgatt taaggaactt ggttctagac atgattcaac tagatccatc
     3361 caaaagactt tcatgtgatg aactactgaa taaatatcgt ggcattttct tccccgatta
     3421 tttctacact ttcatttatg attatttcag aaatttggtt actatgacaa caagcacacc
     3481 gatatcagat aacacttgca ccaatagtac cttggaagac aatgtaaaac ttttagatga
     3541 aactacggaa aaaatataca gagatttttc ccaaatatgt cattgtttgg actttccttt
     3601 aataaaagac gggggtgaga ttggttcaga ccccccaatt ttggaatctt acaaaataga
     3661 gatagaaatt agtcggtttt taaacacaaa cttatatttc ccccaaaatt accatttagt
     3721 cttacagcag tttaccaaag tatccgaaaa gataaaatca gttaaagagg aatgtgcctt
     3781 actctttatc tcttatttgt ctcatagtat aagaagtatt gtttcgactg ctacgaaact
     3841 taaaaattta gaactattag cagtatttgc acaattcgtt tctgatgaaa ataaaattga
     3901 tcgagtcgta ccttatttcg tatgttgttt tgaagatagt gaccaggacg tccaggccct
     3961 atctttgtta acattaatcc aggtactcac ctctgtaaga aaattgaatc aattgaacga
     4021 gaatatattt gtggactact tacttccgag actgaaaaga ttacttattt ccaataggca
     4081 gaataccaat tatttaagga ttgtgtttgc taattgtttg agcgacttgg ccattatcat
     4141 taatagattt caagaattta catttgctca gcactgcaat gataactcaa tggataacaa
     4201 cacggaaatc atggaaagca gtaccaagta ttcagcaaaa ttgatccaaa gtgtcgaaga
     4261 tttaactgtc tcttttttaa cagataatga tacttatgta aagatggcac ttttgcaaaa
     4321 cattcttcca ctttgtaaat tttttggtag ggaaagaaca aacgatatta tactgagtca
     4381 tttaataacc taccttaatg ataaggaccc agcattgcga gtttccttaa ttcaaacaat
     4441 atccggaata tcaattcttt tgggtaccgt tacattagaa cagtatattt taccattgtt
     4501 aatccagacc atcactgatt cggaagaatt agtagtgatc agtgttttac aaagcttaaa
     4561 atctttgttc aagactgggt tgattaggaa aaaatattat attgatatat caaaaacaac
     4621 atctcccttg ttgttgcatc ctaataattg gataagacag tttactttga tgataattat
     4681 agaaattatt aataagttat caaaagccga agtgtactgc attctctatc caataataag
     4741 gcctttcttc gaatttgacg ttgagttcaa cttcaaatca atgataagct gttgcaagca
     4801 accagtgtca agatcggttt acaatctatt gtgtagttgg tctgttagag cgtcaaaatc
     4861 tttattttgg aaaaaaatca tcacaaatca tgtagattca ttcggaaata atagaatcga
     4921 atttataaca aaaaattact caagtaaaaa ttatggattt aataaaagag atacgaaatc
     4981 aagttcctcg ctgaagggta ttaaaacatc atccaccgtc tattcacatg acaacaagga
     5041 aattccctta actgctgaag acagaaattg gattgataag ttccacatta ttgggctaac
     5101 agaaaaagat atttggaaaa ttgtggcttt gaggggttat gtaataagga cagcgagagt
     5161 tatggcagcg aaccctgatt ttccatataa taatagtaat taccgtccat tagtacagaa
     5221 ctcaccacct aacctaaacc ttacgaatat tatgccaaga aacattttct ttgatgtaga
     5281 gtttgctgaa gagtcgacaa gtgagggaca agattctaac ttagagaacc aacagatata
     5341 taaatatgat gaaagtgaga aagatagcaa taagctaaat attaacggta gcaaacagct
     5401 atctaccgtc atggacataa atggatcact aatattcaaa aataagtcca ttgccactac
     5461 tacttctaat ttgaagaacg tttttgttca gttagaacca acgtcctatc acatgcattc
     5521 tccaaatcat ggcttgaaag ataatgcaaa tgttaaacca gaaaggaagg tagtcgtcag
     5581 caacagctat gaaggcgacg ttgaaagcat agaaaaattc ctatcgactt tcaaaatttt
     5641 acctcctctg agagattata aggagtttgg gcctattcaa gagattgtac ggagtccaaa
     5701 catgggtaat ttgaggggca agttgatagc tactttgatg gaaaacgaac ccaattctat
     5761 tacgtcttct gctgtttctc caggagaaac accctattta ataacaggtt cagatcaagg
     5821 tgtaatcaag atttggaacc tgaaagagat tatcgtgggc gaggtttact cttcttcttt
     5881 aacttatgac tgctcctcta ccgtaactca gataaccatg attcctaact ttgacgcgtt
     5941 tgccgtttcc agtaaagatg gacaaataat tgtattaaag gttaatcatt accaacaaga
     6001 aagtgaagtc aaatttttga attgcgaatg catcaggaaa attaacttga agaattttgg
     6061 taaaaatgaa tacgcagtga gaatgagagc atttgtgaat gaggaaaaat ctctactagt
     6121 agcattgacg aatttgtcaa gggttattat atttgatatt agaaccctgg agaggttaca
     6181 aattatagag aattctccaa ggcatggtgc cgtttcaagc atctgtatcg atgaagagtg
     6241 ttgtgtccta attttgggga cgactagagg tattattgat atatgggata tccgtttcaa
     6301 cgtgctgata aggagttggt cctttgggga ccacgcacca atcacgcatg tggaggtttg
     6361 tcagttttat ggaaagaatt ctgtaattgt tgtaggaggt agttcaaaaa catttctaac
     6421 aatatggaat tttgttaagg ggcattgtca gtatgctttc ataaattctg atgaacagcc
     6481 atctatggag cactttttac caattgagaa aggcttagaa gaattaaatt tttgtggaat
     6541 caggtcttta aacgcactaa gcactatctc agtatctaat gataaaattc ttcttaccga
     6601 tgaagcaaca agttccattg ttatgtttag cctaaatgag ctttcttctt ctaaagcagt
     6661 aataagtcct tcaagattca gtgacgtttt tattcctaca caagttacgg caaatctcac
     6721 aatgttattg agaaaaatga aacgtactag cactcattca gtagatgatt ctctatatca
     6781 tcatgatatt ataaattcta tatctacatg tgaagttgat gagacacctt tgctggttgc
     6841 ttgtgataac tcagggctta ttggaatctt ccaaggatcc tctagaccaa ctactgcctc
     6901 tgaaaatttg tactttcaag gcgagttgaa gaccactgct tctgaaaact tgtacttcca
     6961 aggtgagtta ctcgagggtg aattgaagac tgctgctttg gctcaacacg acgaagctag
     7021 atccggagca ggggcgggtg ctagcgagaa cctgtacttc cagggcgccc acgatgaagc
     7081 cgtagacaac aaattcaaca aagaacaaca aaacgcgttc tatgagatct tacatttacc
     7141 taacttaaac gaagaacaac gaaacgcctt catccaaagt ttaaaagatg acccaagcca
     7201 aagcgctaac cttttagcag aagctaaaaa gctaaatgat gctcaggcgc cgaaagtaga
     7261 caacaaattc aacaaagaac aacaaaacgc gttctatgag atcttacatt tacctaactt
     7321 aaacgaagaa caacgaaacg ccttcatcca aagtttaaaa gatgacccaa gccaaagcgc
     7381 taacctttta gcagaagcta aaaagctaaa tgatgctcag gcgccgaaag tagacgcgaa
     7441 ttcgtgagct cgcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa
     7501 aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct
     7561 tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctctta
     7621 ttgaccacac ctctaccggc agcggccgcc accgcggtgg agctccagct tttgttccct
     7681 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7741 ttgttatccg ctcacaattc cacacaacat aggagccgga agcataaagt gtaaagcctg
     7801 gggtgcctaa tgagtgaggt aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7861 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7921 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7981 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8041 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8101 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8161 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8221 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8281 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8341 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8401 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8461 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8521 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     8581 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8641 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8701 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8761 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8821 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8881 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8941 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9001 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9061 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9121 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9181 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9241 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9301 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9361 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9421 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9481 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9541 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9601 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9661 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9721 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9781 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9841 gcgcacattt ccccgaaaag tgccacctga acgaagcatc tgtgcttcat tttgtagaac
     9901 aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag
     9961 aacagaaatg caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt
    10021 aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt
    10081 tacagaacag aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt
    10141 tttgttctac aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac
    10201 tttttttctc ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt
    10261 ccgttaaggt tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg
    10321 actccacttc ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa
    10381 aggcatcccc gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt
    10441 gatagcgttg atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct
    10501 ctatatacta cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa
    10561 tagttcttac tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt
    10621 agaggtcgag tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg
    10681 atatagcaca gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat
    10741 tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc
    10801 ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc tatactttct agagaatagg
    10861 aacttcggaa taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc
    10921 gagctgcgca catacagctc actgttcacg tcgcacctat atctgcgtgt tgcctgtata
    10981 tatatataca tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc
    11041 gtctatttat gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg
    11101 cggggtatcg tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct
    11161 caattggatt agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatactaag
    11221 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
