LOCUS       Exported                9860 bp ds-DNA     circular SYN 04-APR-2012
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9860
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=M13 origin
     rep_origin      complement(1483..1789)
                     /direction=LEFT
                     /label=F1 ori
     CDS             complement(1811..1879)
                     /label=LacZ alpha
     primer_bind     1950..1967
                     /label=M13-fwd
     primer_bind     1976..1996
                     /label=T7
     misc_feature    join(2010..2040,2042..2506)
                     /label=GALGAPDH
     misc_feature    2376..2399
                     /label=galf(opo728)
     misc_feature    join(2512..2910,2912..5062,5064..5454)
                     /label=ScVPS15 (1-981)
     misc_feature    2774..2792
                     /label=LZo39
     misc_feature    2911
                     /label=T134A natural mutation
     misc_feature    3322..3339
                     /label=LZo41
     misc_feature    3932..3949
                     /label=LZo43
     misc_feature    4537..4554
                     /label=LZo45
     misc_feature    5063
                     /label=I851R natural mutation
     misc_feature    5155..5172
                     /label=LZo47
     misc_feature    5461..5604
                     /label=linker
     misc_feature    5482..5502
                     /label=TEV
     misc_feature    5524..5544
                     /label=TEV
                     /label=TEV(1)
     misc_feature    5605..5622
                     /label=GAGA linker
     misc_feature    5623..6024
                     /label=zz
     misc_feature    6028..6221
                     /label=ADH1 teminator
     primer_bind     complement(6256..6275)
                     /label=T3
     primer_bind     complement(6293..6313)
                     /label=M13-rev
     misc_binding    complement(6319..6341)
                     /label=LacO
     rep_origin      complement(6681..7363)
                     /direction=LEFT
                     /label=ColE1 origin
     misc_feature    6743..6761
                     /label=YO032
     CDS             complement(7461..8120)
                     /label=AmpR
     misc_recomb     complement(9413..9446)
                     /label=FRT
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     1981 acgactcact atagggcgaa ttgggtaccg ctatgacatg attacgaatt ggcgcgccgg
     2041 gccgcggatc tttgcaaaga tccaaaaaaa gcatgcgtac ggattagaag ccgccgagcg
     2101 ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt
     2161 cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac aataaagatt ctacaatact
     2221 agcttttatg gttatgaaga ggaaaattgg cagtaacctg gccccacaaa cctcaaatga
     2281 acgaatcaaa ttaacaacca gatatctcga ctgaaaaaaa aggtttaaac cagttccctg
     2341 aaattattcc cctacttgac taataagtat ataaagacgg taggtattga ttgtaattct
     2401 gtaaatctat ttcttaaact tcttgaattc tacttttata gttagtcttt tttttagttt
     2461 taaaacacca agaacttagt ttcgaataaa cacacataaa taaacctcga gatgggggca
     2521 caattatcac tagtggtcca agcatcacct tccatagcca ttttttcata tatcgatgtc
     2581 ttagaggaag tacactacgt ttcacagtta aactcatcaa gattcttaaa aacatgcaag
     2641 gcactggacc ctaacggcga aattgttatc aaagtgttta ttaaaccaaa agaccaatat
     2701 agcttacgac cttttctcca acgtataagg gctcaatcgt ttaagttggg acaactaccg
     2761 cacgttttaa actacagtaa attgatcgag acaaatagag ccggctacat gatacggcag
     2821 cacttaaaaa ataatttata tgacagattg agtttgagac cttacttaca agacattgaa
     2881 ctgaaattca ttgctttcca gttgttaaat gcattaaagg acattcataa tctgaatatt
     2941 gtccatggtg atataaagac agaaaatatc ctagtaacaa gttggaattg gtgtatattg
     3001 acagattttg ctgcatttat caaacccgta tatttgcctg aagataatcc aggtgaattt
     3061 ttattctact tcgacacctc gaagagaaga acctgttatc tagccccgga gaggtttaac
     3121 tctaaacttt accaagatgg aaaatctaac aatggtaggc taactaaaga aatggacata
     3181 tttagtcttg gatgtgttat tgcagaaata tttgctgaag gaagacccat cttcaactta
     3241 tcacagctat tcaaatataa aagtaattca tatgacgtaa acagggaatt tctcatggag
     3301 gaaatgaatt ctaccgattt aaggaacttg gttctagaca tgattcaact agatccatcc
     3361 aaaagacttt catgtgatga actactgaat aaatatcgtg gcattttctt ccccgattat
     3421 ttctacactt tcatttatga ttatttcaga aatttggtta ctatgacaac aagcacaccg
     3481 atatcagata acacttgcac caatagtacc ttggaagaca atgtaaaact tttagatgaa
     3541 actacggaaa aaatatacag agatttttcc caaatatgtc attgtttgga ctttccttta
     3601 ataaaagacg ggggtgagat tggttcagac cccccaattt tggaatctta caaaatagag
     3661 atagaaatta gtcggttttt aaacacaaac ttatatttcc cccaaaatta ccatttagtc
     3721 ttacagcagt ttaccaaagt atccgaaaag ataaaatcag ttaaagagga atgtgcctta
     3781 ctctttatct cttatttgtc tcatagtata agaagtattg tttcgactgc tacgaaactt
     3841 aaaaatttag aactattagc agtatttgca caattcgttt ctgatgaaaa taaaattgat
     3901 cgagtcgtac cttatttcgt atgttgtttt gaagatagtg accaggacgt ccaggcccta
     3961 tctttgttaa cattaatcca ggtactcacc tctgtaagaa aattgaatca attgaacgag
     4021 aatatatttg tggactactt acttccgaga ctgaaaagat tacttatttc caataggcag
     4081 aataccaatt atttaaggat tgtgtttgct aattgtttga gcgacttggc cattatcatt
     4141 aatagatttc aagaatttac atttgctcag cactgcaatg ataactcaat ggataacaac
     4201 acggaaatca tggaaagcag taccaagtat tcagcaaaat tgatccaaag tgtcgaagat
     4261 ttaactgtct cttttttaac agataatgat acttatgtaa agatggcact tttgcaaaac
     4321 attcttccac tttgtaaatt ttttggtagg gaaagaacaa acgatattat actgagtcat
     4381 ttaataacct accttaatga taaggaccca gcattgcgag tttccttaat tcaaacaata
     4441 tccggaatat caattctttt gggtaccgtt acattagaac agtatatttt accattgtta
     4501 atccagacca tcactgattc ggaagaatta gtagtgatca gtgttttaca aagcttaaaa
     4561 tctttgttca agactgggtt gattaggaaa aaatattata ttgatatatc aaaaacaaca
     4621 tctcccttgt tgttgcatcc taataattgg ataagacagt ttactttgat gataattata
     4681 gaaattatta ataagttatc aaaagccgaa gtgtactgca ttctctatcc aataataagg
     4741 cctttcttcg aatttgacgt tgagttcaac ttcaaatcaa tgataagctg ttgcaagcaa
     4801 ccagtgtcaa gatcggttta caatctattg tgtagttggt ctgttagagc gtcaaaatct
     4861 ttattttgga aaaaaatcat cacaaatcat gtagattcat tcggaaataa tagaatcgaa
     4921 tttataacaa aaaattactc aagtaaaaat tatggattta ataaaagaga tacgaaatca
     4981 agttcctcgc tgaagggtat taaaacatca tccaccgtct attcacatga caacaaggaa
     5041 attcccttaa ctgctgaaga cagaaattgg attgataagt tccacattat tgggctaaca
     5101 gaaaaagata tttggaaaat tgtggctttg aggggttatg taataaggac agcgagagtt
     5161 atggcagcga accctgattt tccatataat aatagtaatt accgtccatt agtacagaac
     5221 tcaccaccta acctaaacct tacgaatatt atgccaagaa acattttctt tgatgtagag
     5281 tttgctgaag agtcgacaag tgagggacaa gattctaact tagagaacca acagatatat
     5341 aaatatgatg aaagtgagaa agatagcaat aagctaaata ttaacggtag caaacagcta
     5401 tctaccgtca tggacataaa tggatcacta atattcaaaa ataagtccat tgccggatcc
     5461 tctagaccaa ctactgcctc tgaaaatttg tactttcaag gcgagttgaa gaccactgct
     5521 tctgaaaact tgtacttcca aggtgagtta ctcgagggtg aattgaagac tgctgctttg
     5581 gctcaacacg acgaagctag atccggagca ggggcgggtg ctagcgagaa cctgtacttc
     5641 cagggcgccc acgatgaagc cgtagacaac aaattcaaca aagaacaaca aaacgcgttc
     5701 tatgagatct tacatttacc taacttaaac gaagaacaac gaaacgcctt catccaaagt
     5761 ttaaaagatg acccaagcca aagcgctaac cttttagcag aagctaaaaa gctaaatgat
     5821 gctcaggcgc cgaaagtaga caacaaattc aacaaagaac aacaaaacgc gttctatgag
     5881 atcttacatt tacctaactt aaacgaagaa caacgaaacg ccttcatcca aagtttaaaa
     5941 gatgacccaa gccaaagcgc taacctttta gcagaagcta aaaagctaaa tgatgctcag
     6001 gcgccgaaag tagacgcgaa ttcgtgagct cgcgaatttc ttatgattta tgatttttat
     6061 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt
     6121 aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt
     6181 atagcatgag gtcgctctta ttgaccacac ctctaccggc agcggccgcc accgcggtgg
     6241 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     6301 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat aggagccgga
     6361 agcataaagt gtaaagcctg gggtgcctaa tgagtgaggt aactcacatt aattgcgttg
     6421 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6481 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6541 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6601 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6661 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6721 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6781 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6841 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6901 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6961 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7021 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7081 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     7141 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7201 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7261 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7321 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7381 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7441 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7501 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7561 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7621 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7681 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7741 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7801 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7861 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7921 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7981 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8041 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8101 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8161 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8221 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8281 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8341 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8401 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga acgaagcatc
     8461 tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa
     8521 tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt accaacgaag
     8581 aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
     8641 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa
     8701 caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg ctatttttct
     8761 aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc agtctcttga
     8821 taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt gtctattttc
     8881 tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag cgaagctgcg
     8941 ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg tggattgcgc
     9001 atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa
     9061 cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg
     9121 ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta
     9181 gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg
     9241 gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc
     9301 aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt
     9361 tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc
     9421 tatactttct agagaatagg aacttcggaa taggaacttc aaagcgtttc cgaaaacgag
     9481 cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg tcgcacctat
     9541 atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc gtgtttatgc
     9601 ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta gtacctcctg
     9661 tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac cctttagctg
     9721 ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta tcatttcctt
     9781 tgatattgga tcatactaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     9841 tatcacgagg ccctttcgtc
//
