LOCUS       Exported               20155 bp ds-DNA     circular SYN 14-NOV-2013
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..20155
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    992..994
                     /label=start
     misc_feature    995..1009
                     /label=GAGA linker
     misc_feature    1010..1408
                     /label=proteinA tag x2
     misc_feature    1409..1429
                     /label=TEV cleavage site
     misc_feature    1460..1480
                     /label=TEV
     misc_feature    1502..1522
                     /label=TEV
                     /label=TEV(1)
     misc_feature    1577..1579
                     /label=start
                     /label=start(1)
     misc_feature    1583..3055
                     /label=ATG14L N256S
     misc_feature    2346
                     /label=mutation1
     misc_feature    3062..3064
                     /label=stop
     misc_feature    3377..4007
                     /label=CMV promoter
     misc_feature    4007..4029
                     /label=T7 pro
     primer_bind     4007..4026
                     /label=T7
     misc_feature    4034..4120
                     /label=MCS
     misc_feature    4121..4840
                     /label=EGFP coding
     misc_feature    4841..4846
                     /label=MCS
                     /label=MCS(1)
     primer_bind     complement(4851..4868)
                     /label=SP6
     misc_feature    4870..5101
                     /label=bGH PolyA site
     misc_feature    5154..5880
                     /label=CMV promoter
                     /label=CMV promoter(1)
     misc_feature    5726..5732
                     /label=TATA
     misc_feature    5742..5781
                     /label=x2 TetO2
     misc_feature    join(5889..5911,7266..7290)
                     /label=MCS
                     /label=MCS(2)
     misc_feature    join(5912..7218,7220..7230,7232..7245,7247..7264)
                     /label=Beclin FL
     misc_feature    7308..7532
                     /label=PolA
     misc_feature    7571..8297
                     /label=CMV promoter
                     /label=CMV promoter(2)
     misc_feature    8143..8149
                     /label=TATA
                     /label=TATA(1)
     misc_feature    8159..8198
                     /label=x2 TetO2
                     /label=x2 TetO2(1)
     misc_feature    join(8306..8328,10999..11019)
                     /label=MCS
                     /label=MCS(3)
     misc_feature    8329..8331
                     /label=ATG start
     misc_feature    8335..10989
                     /label=HsVps34-full length (1-887)
     misc_feature    8851..8875
                     /label=LZo110
     misc_feature    9349..9373
                     /label=LZO108
     misc_feature    9900..9923
                     /label=LZo109
     misc_feature    11037..11261
                     /label=PolA
                     /label=PolA(1)
     misc_feature    11300..12026
                     /label=CMV promoter
                     /label=CMV promoter(3)
     misc_feature    11872..11878
                     /label=TATA
                     /label=TATA(2)
     misc_feature    11888..11927
                     /label=x2 TetO2
                     /label=x2 TetO2(2)
     misc_feature    12035..12051
                     /label=MCS
                     /label=MCS(4)
     misc_feature    12052..16125
                     /label=hsVPS15 fl (minus stop)
     misc_feature    12052..12054
                     /label=ATG start
                     /label=ATG start(1)
     misc_feature    12194..12213
                     /label=LZo115r
     misc_feature    12503..12522
                     /label=Synthesised primer 1
     misc_feature    12503..12522
                     /label=OP0635
     misc_feature    13236..13254
                     /label=LZo14
     misc_feature    13621..13649
                     /label=SAM097F
     misc_feature    13621..13649
                     /label=sam097(542-842)
     misc_feature    13621..13649
                     /label=SAM098R
     misc_feature    13621..13649
                     /label=sam098(182-512)
     misc_feature    14946..14967
                     /label=(LZ011)R
     misc_feature    14946..14967
                     /label=LZo15 R
     misc_feature    15526..15553
                     /label=sam0100(869-1148)
     misc_feature    15526..15553
                     /label=SAM0100R
     misc_feature    15526..15553
                     /label=SAM099F
     misc_feature    16130..16154
                     /label=MCS
                     /label=MCS(5)
     misc_feature    16172..16396
                     /label=PolA
                     /label=PolA(2)
     rep_origin      16459..16765
                     /label=F1 ori
     primer_bind     complement(17997..18017)
                     /label=M13-rev
     misc_binding    complement(18023..18045)
                     /label=LacO
     CDS             complement(19162..19821)
                     /label=AmpR
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggaacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg gatgggagca ggggcgggtg ctagcgagaa
     1021 cctgtacttc cagggcgccc acgatgaagc cgtagacaac aaattcaaca aagaacaaca
     1081 aaacgcgttc tatgagatct tacatttacc taacttaaac gaagaacaac gaaacgcctt
     1141 catccaaagt ttaaaagatg acccaagcca aagcgctaac cttttagcag aagctaaaaa
     1201 gctaaatgat gctcaggcgc cgaaagtaga caacaaattc aacaaagaac aacaaaacgc
     1261 gttctatgag atcttacatt tacctaactt aaacgaagaa caacgaaacg ccttcatcca
     1321 aagtttaaaa gatgacccaa gccaaagcgc taacctttta gcagaagcta aaaagctaaa
     1381 tgatgctcag gcgccgaaag tagacgcgga aaacctgtat ttccagggag gcggatcttc
     1441 tagaccaact actgcctctg aaaatttgta ctttcaaggc gagttgaaga ccactgcttc
     1501 tgaaaacttg tacttccaag gtgagttact cgagggtgaa ttgaagactg ctgctttggc
     1561 tcaacacgac gaagccatga cggcgtctcc cagtgggaag ggagcccggg cgctggaggc
     1621 tcctggctgc gggccccggc cgctcgcccg ggacctggtg gactccgtgg acgatgcgga
     1681 ggggctgtac gtggctgtgg agcgctgccc gctgtgcaac actacccgcc ggcggctgac
     1741 ctgcgccaaa tgcgttcaga gcggcgattt cgtctacttc gacggccgcg accgggagag
     1801 gtttatcgac aagaaggaaa ggttaagccg acttaagagc aagcaagaag aatttcagaa
     1861 agaagtgtta aaagctatgg aaggaaaatg gataacagat cagttgagat ggaaaataat
     1921 gtcctgcaag atgaggattg aacagttaaa acaaacaata tgtaaaggaa atgaagaaat
     1981 ggagaaaaat tctgaaggcc ttctcaaaac caaggaaaag aatcagaagc tttacagtcg
     2041 agcacaacgg caccaagaga aaaaggagaa gattcagagg cataatcgca aacttggtga
     2101 cctggtagaa aaaaagacca ttgacttaag aagtcattat gagcgtctgg caaatcttcg
     2161 acgatcccat atattagagc tcacctctgt catttttcca atcgaggaag taaagacggg
     2221 tgtgagagac cccgcagatg tgtcttcaga gagtgacagt gccatgacct ccagcactgt
     2281 gagcaagctt gctgaagccc ggaggacaac ttacctctca ggacgatggg tctgtgacga
     2341 tcacagcgga gacaccagca ttagcattac agggccttgg attagcctcc ctaacaatgg
     2401 ggactactct gcctactaca gctgggtgga ggagaagaaa acaacccagg ggcctgacat
     2461 ggagcagagt aaccctgcct acaccatcag tgctgcgctg tgctatgcaa ctcagctggt
     2521 caacattctg tctcatatac ttgatgtaaa tcttcccaaa aagctctgca acagtgaatt
     2581 ttgtggcgaa aatctaagca agcagaaatt tactcgagca gtgaagaaac tgaatgcaaa
     2641 tattctttac ctttgttttt ctcagcatgt aaatttagat caattacaac cactgcatac
     2701 cctcaggaat ctaatgtacc tggtcagtcc aagctctgaa cacctaggca ggtcagggcc
     2761 ctttgaagta cgagcagacc ttgaggagtc catggaattt gtggatcccg gagttgctgg
     2821 agaatcagat gagagcggag atgagcgcgt cagcgatgaa gaaaccgacc tgggcacaga
     2881 ctgggagaac ttgcctagtc cccggttttg tgatatccct tcccagtctg tggaagtctc
     2941 ccagagtcag agcacccagg cgtccccacc catcgcgagc agcagtgcag gtgggatgat
     3001 ctcctctgca gcagcctcgg tgacctcctg gtttaaagct tacactggac accgtagatc
     3061 ctaacccaag cttaagtcga gtagagggcc cgtttaaacc cgctgatcag cctcgactgt
     3121 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     3181 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     3241 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     3301 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     3361 cagctggggc tctaggttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     3421 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     3481 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     3541 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggactattta cggtaaactg
     3601 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     3661 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     3721 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     3781 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     3841 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     3901 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     3961 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
     4021 atagggagac ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct
     4081 ggaattctgc agatatccat cacactggcg gccgctcgag atggtgagca agggcgagga
     4141 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     4201 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     4261 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     4321 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     4381 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     4441 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     4501 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     4561 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     4621 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     4681 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     4741 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     4801 cgccgggatc actctcggca tggacgagct gtacaagtaa gggccctatt ctatagtgtc
     4861 acctaaatgc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     4921 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     4981 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     5041 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     5101 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggttgaca
     5161 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     5221 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     5281 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     5341 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     5401 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     5461 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     5521 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     5581 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggaa
     5641 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     5701 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctccctatca gtgatagaga
     5761 tctccctatc agtgatagag atcgtcgacg agctcgttta gtgaaccgtc agatcgcctg
     5821 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg
     5881 gactctagcg tttaaactta agcttggtac catggaaggg tctaagacgt ccaacaacag
     5941 caccatgcag gtgagcttcg tgtgccagcg ctgcagccag cccctgaaac tggacacgag
     6001 tttcaagatc ctggaccgtg tcaccatcca ggaactcaca gctccattac ttaccacagc
     6061 ccaggcgaaa ccaggagaga cccaggagga agagactaac tcaggagagg agccatttat
     6121 tgaaactcct cgccaggatg gtgtctctcg cagattcatc cccccagcca ggatgatgtc
     6181 cacagaaagt gccaacagct tcactctgat tggggaggca tctgatggcg gcaccatgga
     6241 gaacctcagc cgaagactga aggtcactgg ggaccttttt gacatcatgt cgggccagac
     6301 agatgtggat cacccactct gtgaggaatg cacagatact cttttagacc agctggacac
     6361 tcagctcaac gtcactgaaa atgagtgtca gaactacaaa cgctgtttgg agatcttaga
     6421 gcaaatgaat gaggatgaca gtgaacagtt acagatggag ctaaaggagc tggcactaga
     6481 ggaggagagg ctgatccagg agctggaaga cgtggaaaag aaccgcaaga tagtggcaga
     6541 aaatctcgag aaggtccagg ctgaggctga gagactggat caggaggaag ctcagtatca
     6601 gagagaatac agtgaattta aacgacagca gctggagctg gatgatgagc tgaagagtgt
     6661 tgaaaaccag atgcgttatg cccagacgca gctggataag ctgaagaaaa ccaacgtctt
     6721 taatgcaacc ttccacatct ggcacagtgg acagtttggc acaatcaata acttcaggct
     6781 gggtcgcctg cccagtgttc ccgtggaatg gaatgagatt aatgctgctt ggggccagac
     6841 tgtgttgctg ctccatgctc tggccaataa gatgggtctg aaatttcaga gataccgact
     6901 tgttccttac ggaaaccatt catatctgga gtctctgaca gacaaatcta aggagctgcc
     6961 gttatactgt tctggggggt tgcggttttt ctgggacaac aagtttgacc atgcaatggt
     7021 ggctttcctg gactgtgtgc agcagttcaa agaagaggtt gagaaaggcg agacacgttt
     7081 ttgtcttccc tacaggatgg atgtggagaa aggcaagatt gaagacacag gaggcagtgg
     7141 cggctcctat tccatcaaaa cccagtttaa ctctgaggag cagtggacaa aagctctcaa
     7201 gttcatgctg acgaatctga agtggggtct ggcttgggtg tcctctcaat tttataacaa
     7261 atgagtcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     7321 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     7381 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     7441 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     7501 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     7561 ctctaggcgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     7621 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     7681 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     7741 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     7801 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     7861 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     7921 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     7981 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     8041 gggagtttgt tttggaacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     8101 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
     8161 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
     8221 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     8281 gaccgatcca gcctccggac tctagcgttt aaacttaagc ttggtaccat gggggaagca
     8341 gagaagtttc actacatcta tagttgtgac ctggatatca acgtccagct taagatagga
     8401 agcttggaag ggaagagaga acaaaagagt tataaagctg tcctggaaga cccaatgttg
     8461 aagttctcag gactatatca agagacatgc tctgatcttt atgttacttg tcaagttttt
     8521 gcagaaggga agcctttggc cttgccagtg agaacatcct acaaagcatt tagtacaaga
     8581 tggaactgga atgaatggct gaaactacca gtaaaatacc ctgacctgcc caggaatgcc
     8641 caagtggccc tcaccatctg ggatgtgtat ggtcccggaa aagcagtgcc tgtaggagga
     8701 acaacggttt cgctctttgg aaaatacggc atgtttcgcc aagggatgca tgacttgaaa
     8761 gtctggccta atgtagaagc agatggatca gaacccacaa aaactcctgg cagaacaagt
     8821 agcactctct cagaagatca gatgagccgt cttgccaagc tcaccaaagc tcatcgacaa
     8881 ggacacatgg tgaaagtaga ttggctggat agattgacat ttagagaaat agaaatgata
     8941 aatgagagtg aaaaacgaag ttctaatttc atgtacctga tggttgaatt tcgatgtgtc
     9001 aagtgtgatg ataaggaata tggtattgtt tattatgaaa aggacggtga tgaatcatct
     9061 ccaattttaa caagttttga attagtgaaa gttcctgacc cccagatgtc tatggagaat
     9121 ttagttgaga gcaaacacca caagcttgcc cggagtttaa gaagtggacc ttctgaccac
     9181 gatctgaaac ccaatgctgc cacgagagat cagttaaata ttattgtgag ttatccacca
     9241 accaagcaac ttacctatga agaacaagat cttgtttgga agtttagata ttatcttacg
     9301 aatcaagaaa aagccttgac aaaattcttg aaatgtgtta attgggatct acctcaagag
     9361 gccaaacagg ccttggaact tctgggaaaa tggaagccga tggatgtaga ggactccttg
     9421 gagctgttat cctctcatta caccaaccca actgtgaggc gttatgctgt tgcccggttg
     9481 cgacaggccg atgatgagga tttgttgatg tacctattac aattggtcca ggctctcaaa
     9541 tatgaaaatt ttgatgatat aaagaatgga ttggaaccta ccaagaagga tagtcagagt
     9601 tcagtgtcag aaaatgtgtc aaattctgga ataaattctg cagaaataga tagctcccaa
     9661 attataacca gcccccttcc ttcagtctct tcacctcctc ctgcatcaaa aacaaaagaa
     9721 gttccagatg gcgaaaatct ggaacaagat ctctgtacct tcttgatatc gagagcctgc
     9781 aaaaactcaa cactggctaa ttatttatac tggtatgtga tagtggaatg tgaagatcaa
     9841 gatactcagc agagagatcc aaagacccat gagatgtact tgaacgtaat gagaagattc
     9901 agccaagcat tgttgaaggg tgataagtct gtcagagtta tgcgttcttt gctggctgca
     9961 caacagacat ttgtagatcg gttggtgcat ctaatgaagg cagtacaacg cgaaagtgga
    10021 aatcgtaaga aaaagaatga gagactacag gcattgcttg gagataatga aaagatgaat
    10081 ttgtcagatg tggaacttat cccgttgcct ttagaacccc aagtgaaaat tagaggaata
    10141 attccggaaa cagctacact gtttaaaagt gcccttatgc ctgcacagtt gttttttaag
    10201 acggaagatg gaggcaaata tccagttata tttaagcatg gagatgattt acgtcaagat
    10261 caacttattc ttcaaatcat ttcactcatg gacaagctgt tacggaaaga aaatctggac
    10321 ttgaaattga caccttataa ggtgttagcc accagtacaa aacatggctt catgcagttt
    10381 atccagtcag ttcctgtggc tgaagttctt gatacagagg gaagcattca gaactttttt
    10441 agaaaatatg caccaagtga gaatgggcca aatgggatta gtgctgaggt catggacact
    10501 tacgttaaaa gctgtgctgg atattgcgtg atcacctata tacttggagt tggagacagg
    10561 cacctggata accttttgct aacaaaaaca ggcaaactct tccacataga ctttggatat
    10621 attctgggtc gtgatccaaa gcctcttcct ccaccaatga agctgaataa agaaatggta
    10681 gaaggaatgg ggggcacaca gagtgagcag taccaagagt tccgtaaaca gtgttacacg
    10741 gctttcctcc acctgcgaag gtattctaat ctgattttga acttgttttc cttgatggtt
    10801 gatgcaaaca ttccagatat tgcacttgaa ccagataaaa ctgtgaaaaa ggttcaggat
    10861 aaattccgct tagacctgtc ggatgaagag gctgtgcatt acatgcagag tctgattgat
    10921 gagagtgtcc atgctctttt tgctgcagtg gtggaacaga ttcacaagtt tgcccagtac
    10981 tggcgcaaat gagtcgaggt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
    11041 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
    11101 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
    11161 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
    11221 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
    11281 cagctggggc tctaggcgcg ttgacattga ttattgacta gttattaata gtaatcaatt
    11341 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
    11401 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
    11461 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
    11521 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
    11581 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
    11641 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
    11701 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
    11761 gacgtcaatg ggagtttgtt ttggaaccaa aatcaacggg actttccaaa atgtcgtaac
    11821 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
    11881 agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct
    11941 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
    12001 agacaccggg accgatccag cctccggact ctagcgttta aacttaagct tatgggaaat
    12061 cagcttgctg gcattgctcc ctcccagatc ctttctgtag agagttattt ttcagatatt
    12121 catgactttg aatatgataa aagcctgggg agtactcggt tttttaaagt tgctcgagcc
    12181 aagcaccgag aaggcctggt cgttgtgaag gtttttgcaa ttcaggatcc cacattgcct
    12241 ttaaccagct ataaacaaga gctggaggaa ctgaaaatca ggcttaattc tgcacagaat
    12301 tgtctacctt tccagaaagc atcagaaaaa gcatctgaga aagcagctat gctctttagg
    12361 cagtatgtgc gagacaatct ctatgatcgc atcagtaccc gtccattctt gaataacatt
    12421 gagaagcgct ggattgcttt ccagatcctg acagctgtgg accaagcaca caaatctgga
    12481 gttcgtcatg gggacatcaa gactgagaat gtgatggtca ccagttggaa ttgggttctt
    12541 ctaactgatt ttgccagttt taagcccact tatcttccag aagacaaccc ggcagatttc
    12601 aattatttct ttgacacatc acggaggaga acttgctata ttgctcctga acgttttgtt
    12661 gatggtggga tgtttgccac tgagttagaa tatatgagag atccttcaac tccgcttgta
    12721 gacttaaata gcaatcagag aacaagagga gagttgaaga gagcaatgga catcttttca
    12781 gcaggttgtg tgatagctga gctttttaca gaaggtgtac cattatttga tctctctcaa
    12841 cttttggctt atagaaatgg acattttttc cctgaacaag tgctaaataa aattgaagat
    12901 cacagtatca gagaattggt aactcagatg attcaccgtg agccagataa acgtttagag
    12961 gcagaagatt acttaaaaca gcagcgtggc aatgcctttc ctgaaatatt ttacactttt
    13021 cttcagccct acatggccca gtttgccaag gaaacgtttc tttctgcaga tgagcgtatt
    13081 ctggttatac ggaaggattt gggcaacatt attcacaatc tctgtggaca tgatctgcca
    13141 gaaaaagccg aaggagagcc taaggaaaat gggctggtta tcttggtatc tgttataaca
    13201 tcctgcctac agacccttaa atactgtgat tccaaactag ctgctttgga actgattctt
    13261 catttggctc caagattaag tgttgaaatc cttttggatc gtattactcc atatcttttg
    13321 catttcagca atgactctgt tcctagggtg agggctgaag ccttgaggac gttgaccaaa
    13381 gttcttgctc tcgtcaaaga ggttcctcgt aatgatatca atatttatcc ggaatacatt
    13441 ctgccaggca tagcccactt agcccaagat gatgctacta tcgttagact agcctatgct
    13501 gaaaacatag ctctgctggc agaaacagct ctgagattcc tggaattagt acagttaaaa
    13561 aatcttaata tggaaaatga ccccaataat gaagaaatag atgaggttac acatccaaat
    13621 ggaaattatg acacagagct gcaagcctta catgaaatgg tccagcagaa agttgttact
    13681 ttgctaagtg accctgaaaa tattgtaaaa caaaccttga tggaaaatgg aataacacgg
    13741 ctgtgtgtat tctttggacg tcagaaagcc aacgatgttt tgttgtccca catgattact
    13801 ttcctaaatg ataagaatga ttggcatcta cgtggagcat tttttgatag tatagttggt
    13861 gttgctgcct atgttggctg gcaaagctcc tcaattctca agcctctgct gcaacaaggt
    13921 cttagtgatg ctgaggaatt tgtcattgtg aaagctcttt atgcccttac ttgtatgtgc
    13981 cagttaggac tgctacaaaa accccatgtt tacgaatttg ccagtgatat tgcccccttc
    14041 ctgtgtcatc ccaatttatg gatacgttat ggtgccgtgg gatttatcac agtggtagct
    14101 cgtcaaataa gtacagctga tgtctactgt aaactgatgc cttatcttga cccatatatt
    14161 acccaaccaa taatacagat tgaaagaaaa cttgttctgc tcagtgtttt aaaggaacca
    14221 gtaagtcgtt ctatatttga ttatgctttg aggtctaaag atattactag cttgttcaga
    14281 catcttcaca tgcgtcagaa gaaacgaaat ggttctcttc ccgactgccc tccgccagag
    14341 gatcctgcca tagcacagct tctgaagaag ttgctctcac agggaatgac agaggaagag
    14401 gaagacaaac ttctggcact gaaagacttc atgatgaaat ctaataaagc aaaggccaat
    14461 atagtggacc agagccatct tcatgatagt agtcagaaag gtgtaattga cttggcagct
    14521 ttaggcataa ctgggagaca agttgatctt gttaaaacca aacaagaacc agatgacaaa
    14581 cgggccagaa aacatgtaaa acaagactca aatgtaaatg aagaatggaa aagcatgttt
    14641 gggtcactgg acccaccaaa catgccacag gccctaccta aagggagtga tcaggaggtg
    14701 attcagactg ggaaacctcc tcgttccgag tcctctgctg gcatttgtgt ccctttgtca
    14761 acttcttcac aggttccaga agtgacaact gtccaaaata aaaaaccagt aataccggtt
    14821 ttaagtagta caatcttacc atccacctat cagattcgaa ttacaacttg taaaactgaa
    14881 cttcagcaac tcatccagca aaagcgggag cagtgcaatg ctgagagaat agctaagcag
    14941 atgatggaaa atgctgaatg ggagagtaaa ccaccaccac ctggatggcg tcctaaaggg
    15001 ctgttagttg cccatcttca tgagcataaa tctgctgtga atcgaattag agtctctgat
    15061 gaacactcac tttttgcaac atgttcaaat gatggcacag tgaaaatctg gaacagtcaa
    15121 aagatggagg ggaagaccac cactaccaga tctattctta catacagccg aattggagga
    15181 cgagtcaaga cgctcacatt ctgccaaggc tcccactatt tagccatagc atctgataat
    15241 ggtgctgtcc agcttcttgg aattgaggct tctaagctgc ccaagtctcc taaaatccat
    15301 cctctacaaa gcagaattct agatcagaag gaggacggtt gtgttgtgga tatgcatcac
    15361 ttcaactctg gagcacagtc tgttcttgcc tatgccactg tgaatggctc tctggttggc
    15421 tgggacctta ggtcttcaag caatgcgtgg actttaaagc atgatttaaa gtcgggcctc
    15481 atcacttcct ttgctgtgga catccaccaa tgctggctct gcattggtac aagcagtggc
    15541 accatggctt gttgggacat gaggttccag ttgccaattt caagtcactg tcatccttcc
    15601 agggctcgaa tcagacgcct ctcaatgcac cctctgtatc agtcctgggt gattgcagct
    15661 gttcagggca acaacgaagt gtccatgtgg gacatggaga ctggtgacag aagatttact
    15721 ctctgggcca gcagtgcacc accactttct gaattacagc cttctcctca tagcgtccat
    15781 ggtatctact gtagtcctgc agatggaaat cctatcctac taacagctgg ctcagatatg
    15841 aaaataaggt tttgggactt ggcttaccca gaaaggtcct atgttgttgc aggaagtact
    15901 agttccccat ctgtgtccta ctacaggaaa ataattgaag gcactgaagt tgtccaggaa
    15961 attcagaata agcagaaagt aggaccaagt gatgacaccc ctcgaagggg cccagagtcc
    16021 ctgcccgtgg gacatcatga catcatcact gatgtcgcca cattccagac cacacagggc
    16081 ttcatcgtaa ctgcttctag agatgggatt gtgaaggtgt ggaaataagt cgagtctaga
    16141 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
    16201 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
    16261 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
    16321 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
    16381 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
    16441 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
    16501 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
    16561 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
    16621 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
    16681 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
    16741 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
    16801 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
    16861 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
    16921 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
    16981 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
    17041 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
    17101 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
    17161 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
    17221 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
    17281 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
    17341 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
    17401 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
    17461 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
    17521 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
    17581 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
    17641 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
    17701 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
    17761 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
    17821 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    17881 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    17941 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
    18001 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    18061 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    18121 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    18181 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    18241 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    18301 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    18361 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    18421 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    18481 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    18541 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    18601 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    18661 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    18721 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    18781 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    18841 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    18901 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca
    18961 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    19021 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    19081 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    19141 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    19201 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    19261 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    19321 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    19381 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    19441 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    19501 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    19561 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    19621 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    19681 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    19741 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    19801 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    19861 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    19921 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    19981 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    20041 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    20101 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
