LOCUS       Exported               22135 bp ds-DNA     circular SYN 20-NOV-2013
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Serial Cloner Genbank Format  SerialCloner_Type=DNA  
            SerialCloner_Comments=Ligation of : pMO13(NRBF2+EGFP+Beclin+hsVP 
            [18962  nt] : (Ml uI-Blunt(filled)[228] / MluI-Blunt(filled)[228]) 
            to PCR(pYO3 07(zz-ATG14L in pcnd4TO).ape.xdna) [3195  nt] : (Uncut 
            5'-Bl unt(trimmed)[0] / Uncut 3'-Blunt(trimmed)[3195])  
            SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     source          1..22135
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    177..195
                     /label=op0829
     misc_feature    215..236
                     /label=XXXXX
     misc_feature    240..966
                     /label=CMV promoter
     promoter        240..966
                     /label=CMV promoter
                     /label=CMV promoter(5)
     misc_feature    812..818
                     /label=TATA
     misc_feature    828..867
                     /label=x2 TetO2
     misc_feature    975..991
                     /label=MCS(4)
     misc_feature    1000..1002
                     /label=start
     misc_feature    1003..1017
                     /label=GAGA linker
     misc_feature    1018..1416
                     /label=proteinA tag x2
     misc_feature    1417..1437
                     /label=TEV cleavage site
     misc_feature    1468..1488
                     /label=TEV
     misc_feature    1510..1530
                     /label=TEV(1)
     misc_feature    1585..3072
                     /label=ATG14L (N265S)
     misc_feature    1585..1587
                     /label=start(1)
     misc_feature    1591..3063
                     /label=ATG14L N256S
     misc_feature    2354
                     /label=mutation1
     misc_feature    3070..3072
                     /label=stop
     misc_feature    3125..3349
                     /label=PolA
     misc_feature    3413..4139
                     /label=CMV promoter
                     /label=CMV promoter(1)
     promoter        3413..4139
                     /label=CMV promoter
                     /label=CMV promoter(6)
     misc_feature    3985..3991
                     /label=TATA
                     /label=TATA(1)
     misc_feature    4001..4040
                     /label=x2 TetO2
                     /label=x2 TetO2(1)
     misc_feature    4148..4164
                     /label=MCS(4)
                     /label=MCS(4)(1)
     misc_feature    4159..4183
                     /label=MOo8F
     misc_feature    4165..5028
                     /label=HsNRBF2 cDNA
     misc_feature    4690..4708
                     /label=MOo12F
     misc_feature    4755..4774
                     /label=MOo13R
     misc_feature    5072..5296
                     /label=PolA
                     /label=PolA(1)
     misc_feature    5361..5991
                     /label=CMV promoter(1)
                     /label=CMV promoter(1)(1)
     misc_feature    5932..5938
                     /label=TATA
                     /label=TATA(2)
     misc_feature    5991..6013
                     /label=T7 pro
     primer_bind     5991..6010
                     /label=T7
     misc_feature    6018..6104
                     /label=MCS
     misc_feature    6105..6824
                     /label=EGFP coding
     misc_feature    6825..6830
                     /label=MCS(1)
     primer_bind     complement(6835..6852)
                     /label=SP6
     misc_feature    6854..7085
                     /label=bGH PolyA site
     misc_feature    6878..7102
                     /label=PolA
                     /label=PolA(2)
     misc_feature    7138..7864
                     /label=CMV promoter
                     /label=CMV promoter(2)
     misc_feature    7710..7716
                     /label=TATA
                     /label=TATA(3)
     misc_feature    7726..7765
                     /label=x2 TetO2
                     /label=x2 TetO2(2)
     misc_feature    7873..7889
                     /label=MCS(4)
                     /label=MCS(4)(2)
     CDS             7896..9248
                     /label=BECLIN
     misc_feature    9292..9516
                     /label=PolA
                     /label=PolA(3)
     misc_feature    9555..10281
                     /label=CMV promoter
                     /label=CMV promoter(3)
     misc_feature    10127..10133
                     /label=TATA
                     /label=TATA(4)
     misc_feature    10143..10182
                     /label=x2 TetO2
                     /label=x2 TetO2(3)
     misc_feature    10290..10306
                     /label=MCS(4)
                     /label=MCS(4)(3)
     misc_feature    10313..10315
                     /label=ATG start
     misc_feature    10319..12973
                     /label=HsVps34
     misc_feature    10835..10859
                     /label=LZo110
     misc_feature    11333..11357
                     /label=LZO108
     misc_feature    11884..11907
                     /label=LZo109
     misc_feature    13021..13245
                     /label=PolA
                     /label=PolA(4)
     misc_feature    13284..14010
                     /label=CMV promoter
                     /label=CMV promoter(4)
     misc_feature    13856..13862
                     /label=TATA
                     /label=TATA(5)
     misc_feature    13872..13911
                     /label=x2 TetO2
                     /label=x2 TetO2(4)
     misc_feature    14019..14035
                     /label=MCS(4)
                     /label=MCS(4)(4)
     misc_feature    14036..18112
                     /label=hsVPS15
     misc_feature    14036..14038
                     /label=ATG start(1)
     misc_feature    14178..14197
                     /label=LZo115r
     misc_feature    14487..14506
                     /label=Synthesised primer 1
     misc_feature    14487..14506
                     /label=OP0635
     misc_feature    15220..15238
                     /label=LZo14
     misc_feature    15605..15633
                     /label=SAM097F
     misc_feature    15605..15633
                     /label=sam097(542-842)
     misc_feature    15605..15633
                     /label=SAM098R
     misc_feature    15605..15633
                     /label=sam098(182-512)
     misc_feature    16930..16951
                     /label=(LZ011)R
     misc_feature    16930..16951
                     /label=LZo15 R
     misc_feature    17510..17537
                     /label=sam0100(869-1148)
     misc_feature    17510..17537
                     /label=SAM0100R
     misc_feature    17510..17537
                     /label=SAM099F
     misc_feature    18113..18138
                     /label=MCS(5)
     misc_feature    18156..18380
                     /label=PolA
                     /label=PolA(5)
     rep_origin      18443..18749
                     /label=F1 ori
     primer_bind     complement(19981..20001)
                     /label=M13-rev
     misc_binding    complement(20007..20029)
                     /label=LacO
     CDS             complement(21146..21805)
                     /label=AmpR
ORIGIN
        1 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
       61 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
      121 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
      181 ctgcttaggg ttaggcgttt tgcgctgctt cgcgcgcgat gtacgggcca gatatacgcg
      241 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
      301 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      361 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      421 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      481 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      541 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      601 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      661 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      721 ttggaaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      781 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctcc ctatcagtga
      841 tagagatctc cctatcagtg atagagatcg tcgacgagct cgtttagtga accgtcagat
      901 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
      961 cctccggact ctagcgttta aacttaagct tggtaccgga tgggagcagg ggcgggtgct
     1021 agcgagaacc tgtacttcca gggcgcccac gatgaagccg tagacaacaa attcaacaaa
     1081 gaacaacaaa acgcgttcta tgagatctta catttaccta acttaaacga agaacaacga
     1141 aacgccttca tccaaagttt aaaagatgac ccaagccaaa gcgctaacct tttagcagaa
     1201 gctaaaaagc taaatgatgc tcaggcgccg aaagtagaca acaaattcaa caaagaacaa
     1261 caaaacgcgt tctatgagat cttacattta cctaacttaa acgaagaaca acgaaacgcc
     1321 ttcatccaaa gtttaaaaga tgacccaagc caaagcgcta accttttagc agaagctaaa
     1381 aagctaaatg atgctcaggc gccgaaagta gacgcggaaa acctgtattt ccagggaggc
     1441 ggatcttcta gaccaactac tgcctctgaa aatttgtact ttcaaggcga gttgaagacc
     1501 actgcttctg aaaacttgta cttccaaggt gagttactcg agggtgaatt gaagactgct
     1561 gctttggctc aacacgacga agccatgacg gcgtctccca gtgggaaggg agcccgggcg
     1621 ctggaggctc ctggctgcgg gccccggccg ctcgcccggg acctggtgga ctccgtggac
     1681 gatgcggagg ggctgtacgt ggctgtggag cgctgcccgc tgtgcaacac tacccgccgg
     1741 cggctgacct gcgccaaatg cgttcagagc ggcgatttcg tctacttcga cggccgcgac
     1801 cgggagaggt ttatcgacaa gaaggaaagg ttaagccgac ttaagagcaa gcaagaagaa
     1861 tttcagaaag aagtgttaaa agctatggaa ggaaaatgga taacagatca gttgagatgg
     1921 aaaataatgt cctgcaagat gaggattgaa cagttaaaac aaacaatatg taaaggaaat
     1981 gaagaaatgg agaaaaattc tgaaggcctt ctcaaaacca aggaaaagaa tcagaagctt
     2041 tacagtcgag cacaacggca ccaagagaaa aaggagaaga ttcagaggca taatcgcaaa
     2101 cttggtgacc tggtagaaaa aaagaccatt gacttaagaa gtcattatga gcgtctggca
     2161 aatcttcgac gatcccatat attagagctc acctctgtca tttttccaat cgaggaagta
     2221 aagacgggtg tgagagaccc cgcagatgtg tcttcagaga gtgacagtgc catgacctcc
     2281 agcactgtga gcaagcttgc tgaagcccgg aggacaactt acctctcagg acgatgggtc
     2341 tgtgacgatc acagcggaga caccagcatt agcattacag ggccttggat tagcctccct
     2401 aacaatgggg actactctgc ctactacagc tgggtggagg agaagaaaac aacccagggg
     2461 cctgacatgg agcagagtaa ccctgcctac accatcagtg ctgcgctgtg ctatgcaact
     2521 cagctggtca acattctgtc tcatatactt gatgtaaatc ttcccaaaaa gctctgcaac
     2581 agtgaatttt gtggcgaaaa tctaagcaag cagaaattta ctcgagcagt gaagaaactg
     2641 aatgcaaata ttctttacct ttgtttttct cagcatgtaa atttagatca attacaacca
     2701 ctgcataccc tcaggaatct aatgtacctg gtcagtccaa gctctgaaca cctaggcagg
     2761 tcagggccct ttgaagtacg agcagacctt gaggagtcca tggaatttgt ggatcccgga
     2821 gttgctggag aatcagatga gagcggagat gagcgcgtca gcgatgaaga aaccgacctg
     2881 ggcacagact gggagaactt gcctagtccc cggttttgtg atatcccttc ccagtctgtg
     2941 gaagtctccc agagtcagag cacccaggcg tccccaccca tcgcgagcag cagtgcaggt
     3001 gggatgatct cctctgcagc agcctcggtg acctcctggt ttaaagctta cactggacac
     3061 cgtagatcct aacccaagct taagtcgagt agagggcccg tttaaacccg ctgatcagcc
     3121 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     3181 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     3241 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     3301 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     3361 gaaagaacca gctggggctc taggcgcgtt gacattgatt attgactagc gcgttgacat
     3421 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     3481 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     3541 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     3601 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     3661 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     3721 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     3781 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     3841 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggaac
     3901 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     3961 ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag tgatagagat
     4021 ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca gatcgcctgg
     4081 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
     4141 actctagcgt ttaaacttaa gcttatggaa gtaatggaag gacccctcaa cctggctcat
     4201 caacagagca gacgagcaga ccgtttatta gctgcaggca aatacgaaga ggctatttct
     4261 tgtcacaaaa aggctgcagc atatctttct gaagccatga agctgacaca gtcagagcag
     4321 gctcatcttt cactggaatt gcaaagggat agccatatga aacagctcct cctcatccaa
     4381 gagagatgga aaagggccca gcgtgaagaa agattgaaag cccagcagaa cacagacaag
     4441 gatgcagctg cccatcttca gacatctcac aaaccctctg cagaggatgc agagggccag
     4501 agtccccttt ctcagaagta cagcccttcc acagagaaat gcctgcctga gattcagggg
     4561 atctttgaca gggatccaga cacactactt tatttacttc agcaaaagag tgagccagca
     4621 gagccatgta ttggaagcaa agccccaaaa gatgataaaa caattataga ggagcaggca
     4681 accaaaattg cagatttgaa gaggcatgtg gaattccttg tggctgagaa tgaaagatta
     4741 aggaaagaaa ataaacaact aaaggctgaa aaggccagac ttctaaaagg tccaatagaa
     4801 aaggagctgg atgtagatgc tgattttgta gaaacgtcag agttatggag cttgccacca
     4861 catgcagaaa ctgctacagc ctcctcaacc tggcagaagt tcgcagcaaa tactgggaaa
     4921 gccaaggaca ttccaatccc caatcttcct cccttggatt ttccatctcc agaacttcct
     4981 cttatggagc tctctgagga tattctgaaa ggatttatga ataattaagt cgagtctaga
     5041 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     5101 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     5161 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     5221 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     5281 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag gcgcgttgac
     5341 attgattatt gactagcgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     5401 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     5461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     5521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggacta tttacggtaa
     5581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     5641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     5701 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     5761 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     5821 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     5881 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     5941 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
     6001 cactataggg agacccaagc ttggtaccga gctcggatcc actagtaacg gccgccagtg
     6061 tgctggaatt ctgcagatat ccatcacact ggcggccgct cgagatggtg agcaagggcg
     6121 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     6181 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     6241 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     6301 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     6361 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     6421 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     6481 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     6541 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     6601 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     6661 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     6721 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     6781 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaagggccc tattctatag
     6841 tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     6901 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     6961 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     7021 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     7081 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctagggtt
     7141 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     7201 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     7261 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     7321 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     7381 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     7441 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     7501 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     7561 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     7621 ggaaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     7681 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
     7741 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
     7801 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
     7861 tccggactct agcgtttaaa cttaagcttg gtaccatgga agggtctaag acgtccaaca
     7921 acagcaccat gcaggtgagc ttcgtgtgcc agcgctgcag ccagcccctg aaactggaca
     7981 cgagtttcaa gatcctggac cgtgtcacca tccaggaact cacagctcca ttacttacca
     8041 cagcccaggc gaaaccagga gagacccagg aggaagagac taactcagga gaggagccat
     8101 ttattgaaac tcctcgccag gatggtgtct ctcgcagatt catcccccca gccaggatga
     8161 tgtccacaga aagtgccaac agcttcactc tgattgggga ggcatctgat ggcggcacca
     8221 tggagaacct cagccgaaga ctgaaggtca ctggggacct ttttgacatc atgtcgggcc
     8281 agacagatgt ggatcaccca ctctgtgagg aatgcacaga tactctttta gaccagctgg
     8341 acactcagct caacgtcact gaaaatgagt gtcagaacta caaacgctgt ttggagatct
     8401 tagagcaaat gaatgaggat gacagtgaac agttacagat ggagctaaag gagctggcac
     8461 tagaggagga gaggctgatc caggagctgg aagacgtgga aaagaaccgc aagatagtgg
     8521 cagaaaatct cgagaaggtc caggctgagg ctgagagact ggatcaggag gaagctcagt
     8581 atcagagaga atacagtgaa tttaaacgac agcagctgga gctggatgat gagctgaaga
     8641 gtgttgaaaa ccagatgcgt tatgcccaga cgcagctgga taagctgaag aaaaccaacg
     8701 tctttaatgc aaccttccac atctggcaca gtggacagtt tggcacaatc aataacttca
     8761 ggctgggtcg cctgcccagt gttcccgtgg aatggaatga gattaatgct gcttggggcc
     8821 agactgtgtt gctgctccat gctctggcca ataagatggg tctgaaattt cagagatacc
     8881 gacttgttcc ttacggaaac cattcatatc tggagtctct gacagacaaa tctaaggagc
     8941 tgccgttata ctgttctggg gggttgcggt ttttctggga caacaagttt gaccatgcaa
     9001 tggtggcttt cctggactgt gtgcagcagt tcaaagaaga ggttgagaaa ggcgagacac
     9061 gtttttgtct tccctacagg atggatgtgg agaaaggcaa gattgaagac acaggaggca
     9121 gtggcggctc ctattccatc aaaacccagt ttaactctga ggagcagtgg acaaaagctc
     9181 tcaagttcat gctgacgaat ctgaagtggg gtctggcttg ggtgtcctct caattttata
     9241 acaaatgagt cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     9301 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     9361 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     9421 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     9481 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     9541 ggggctctag gcgcgttgac attgattatt gactagttat taatagtaat caattacggg
     9601 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     9661 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     9721 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     9781 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     9841 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     9901 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     9961 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
    10021 caatgggagt ttgttttgga accaaaatca acgggacttt ccaaaatgtc gtaacaactc
    10081 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
    10141 tctccctatc agtgatagag atctccctat cagtgataga gatcgtcgac gagctcgttt
    10201 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
    10261 ccgggaccga tccagcctcc ggactctagc gtttaaactt aagcttggta ccatggggga
    10321 agcagagaag tttcactaca tctatagttg tgacctggat atcaacgtcc agcttaagat
    10381 aggaagcttg gaagggaaga gagaacaaaa gagttataaa gctgtcctgg aagacccaat
    10441 gttgaagttc tcaggactat atcaagagac atgctctgat ctttatgtta cttgtcaagt
    10501 ttttgcagaa gggaagcctt tggccttgcc agtgagaaca tcctacaaag catttagtac
    10561 aagatggaac tggaatgaat ggctgaaact accagtaaaa taccctgacc tgcccaggaa
    10621 tgcccaagtg gccctcacca tctgggatgt gtatggtccc ggaaaagcag tgcctgtagg
    10681 aggaacaacg gtttcgctct ttggaaaata cggcatgttt cgccaaggga tgcatgactt
    10741 gaaagtctgg cctaatgtag aagcagatgg atcagaaccc acaaaaactc ctggcagaac
    10801 aagtagcact ctctcagaag atcagatgag ccgtcttgcc aagctcacca aagctcatcg
    10861 acaaggacac atggtgaaag tagattggct ggatagattg acatttagag aaatagaaat
    10921 gataaatgag agtgaaaaac gaagttctaa tttcatgtac ctgatggttg aatttcgatg
    10981 tgtcaagtgt gatgataagg aatatggtat tgtttattat gaaaaggacg gtgatgaatc
    11041 atctccaatt ttaacaagtt ttgaattagt gaaagttcct gacccccaga tgtctatgga
    11101 gaatttagtt gagagcaaac accacaagct tgcccggagt ttaagaagtg gaccttctga
    11161 ccacgatctg aaacccaatg ctgccacgag agatcagtta aatattattg tgagttatcc
    11221 accaaccaag caacttacct atgaagaaca agatcttgtt tggaagttta gatattatct
    11281 tacgaatcaa gaaaaagcct tgacaaaatt cttgaaatgt gttaattggg atctacctca
    11341 agaggccaaa caggccttgg aacttctggg aaaatggaag ccgatggatg tagaggactc
    11401 cttggagctg ttatcctctc attacaccaa cccaactgtg aggcgttatg ctgttgcccg
    11461 gttgcgacag gccgatgatg aggatttgtt gatgtaccta ttacaattgg tccaggctct
    11521 caaatatgaa aattttgatg atataaagaa tggattggaa cctaccaaga aggatagtca
    11581 gagttcagtg tcagaaaatg tgtcaaattc tggaataaat tctgcagaaa tagatagctc
    11641 ccaaattata accagccccc ttccttcagt ctcttcacct cctcctgcat caaaaacaaa
    11701 agaagttcca gatggcgaaa atctggaaca agatctctgt accttcttga tatcgagagc
    11761 ctgcaaaaac tcaacactgg ctaattattt atactggtat gtgatagtgg aatgtgaaga
    11821 tcaagatact cagcagagag atccaaagac ccatgagatg tacttgaacg taatgagaag
    11881 attcagccaa gcattgttga agggtgataa gtctgtcaga gttatgcgtt ctttgctggc
    11941 tgcacaacag acatttgtag atcggttggt gcatctaatg aaggcagtac aacgcgaaag
    12001 tggaaatcgt aagaaaaaga atgagagact acaggcattg cttggagata atgaaaagat
    12061 gaatttgtca gatgtggaac ttatcccgtt gcctttagaa ccccaagtga aaattagagg
    12121 aataattccg gaaacagcta cactgtttaa aagtgccctt atgcctgcac agttgttttt
    12181 taagacggaa gatggaggca aatatccagt tatatttaag catggagatg atttacgtca
    12241 agatcaactt attcttcaaa tcatttcact catggacaag ctgttacgga aagaaaatct
    12301 ggacttgaaa ttgacacctt ataaggtgtt agccaccagt acaaaacatg gcttcatgca
    12361 gtttatccag tcagttcctg tggctgaagt tcttgataca gagggaagca ttcagaactt
    12421 ttttagaaaa tatgcaccaa gtgagaatgg gccaaatggg attagtgctg aggtcatgga
    12481 cacttacgtt aaaagctgtg ctggatattg cgtgatcacc tatatacttg gagttggaga
    12541 caggcacctg gataaccttt tgctaacaaa aacaggcaaa ctcttccaca tagactttgg
    12601 atatattctg ggtcgtgatc caaagcctct tcctccacca atgaagctga ataaagaaat
    12661 ggtagaagga atggggggca cacagagtga gcagtaccaa gagttccgta aacagtgtta
    12721 cacggctttc ctccacctgc gaaggtattc taatctgatt ttgaacttgt tttccttgat
    12781 ggttgatgca aacattccag atattgcact tgaaccagat aaaactgtga aaaaggttca
    12841 ggataaattc cgcttagacc tgtcggatga agaggctgtg cattacatgc agagtctgat
    12901 tgatgagagt gtccatgctc tttttgctgc agtggtggaa cagattcaca agtttgccca
    12961 gtactggcgc aaatgagtcg aggtctagag ggcccgttta aacccgctga tcagcctcga
    13021 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
    13081 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
    13141 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
    13201 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
    13261 gaaccagctg gggctctagg cgcgttgaca ttgattattg actagttatt aatagtaatc
    13321 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
    13381 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
    13441 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
    13501 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
    13561 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
    13621 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
    13681 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
    13741 cattgacgtc aatgggagtt tgttttggaa ccaaaatcaa cgggactttc caaaatgtcg
    13801 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
    13861 aagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag atcgtcgacg
    13921 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
    13981 tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta agcttatggg
    14041 aaatcagctt gctggcattg ctccctccca gatcctttct gtagagagtt atttttcaga
    14101 tattcatgac tttgaatatg ataaaagcct ggggagtact cggtttttta aagttgctcg
    14161 agccaagcac cgagaaggcc tggtcgttgt gaaggttttt gcaattcagg atcccacatt
    14221 gcctttaacc agctataaac aagagctgga ggaactgaaa atcaggctta attctgcaca
    14281 gaattgtcta cctttccaga aagcatcaga aaaagcatct gagaaagcag ctatgctctt
    14341 taggcagtat gtgcgagaca atctctatga tcgcatcagt acccgtccat tcttgaataa
    14401 cattgagaag cgctggattg ctttccagat cctgacagct gtggaccaag cacacaaatc
    14461 tggagttcgt catggggaca tcaagactga gaatgtgatg gtcaccagtt ggaattgggt
    14521 tcttctaact gattttgcca gttttaagcc cacttatctt ccagaagaca acccggcaga
    14581 tttcaattat ttctttgaca catcacggag gagaacttgc tatattgctc ctgaacgttt
    14641 tgttgatggt gggatgtttg ccactgagtt agaatatatg agagatcctt caactccgct
    14701 tgtagactta aatagcaatc agagaacaag aggagagttg aagagagcaa tggacatctt
    14761 ttcagcaggt tgtgtgatag ctgagctttt tacagaaggt gtaccattat ttgatctctc
    14821 tcaacttttg gcttatagaa atggacattt tttccctgaa caagtgctaa ataaaattga
    14881 agatcacagt atcagagaat tggtaactca gatgattcac cgtgagccag ataaacgttt
    14941 agaggcagaa gattacttaa aacagcagcg tggcaatgcc tttcctgaaa tattttacac
    15001 ttttcttcag ccctacatgg cccagtttgc caaggaaacg tttctttctg cagatgagcg
    15061 tattctggtt atacggaagg atttgggcaa cattattcac aatctctgtg gacatgatct
    15121 gccagaaaaa gccgaaggag agcctaagga aaatgggctg gttatcttgg tatctgttat
    15181 aacatcctgc ctacagaccc ttaaatactg tgattccaaa ctagctgctt tggaactgat
    15241 tcttcatttg gctccaagat taagtgttga aatccttttg gatcgtatta ctccatatct
    15301 tttgcatttc agcaatgact ctgttcctag ggtgagggct gaagccttga ggacgttgac
    15361 caaagttctt gctctcgtca aagaggttcc tcgtaatgat atcaatattt atccggaata
    15421 cattctgcca ggcatagccc acttagccca agatgatgct actatcgtta gactagccta
    15481 tgctgaaaac atagctctgc tggcagaaac agctctgaga ttcctggaat tagtacagtt
    15541 aaaaaatctt aatatggaaa atgaccccaa taatgaagaa atagatgagg ttacacatcc
    15601 aaatggaaat tatgacacag agctgcaagc cttacatgaa atggtccagc agaaagttgt
    15661 tactttgcta agtgaccctg aaaatattgt aaaacaaacc ttgatggaaa atggaataac
    15721 acggctgtgt gtattctttg gacgtcagaa agccaacgat gttttgttgt cccacatgat
    15781 tactttccta aatgataaga atgattggca tctacgtgga gcattttttg atagtatagt
    15841 tggtgttgct gcctatgttg gctggcaaag ctcctcaatt ctcaagcctc tgctgcaaca
    15901 aggtcttagt gatgctgagg aatttgtcat tgtgaaagct ctttatgccc ttacttgtat
    15961 gtgccagtta ggactgctac aaaaacccca tgtttacgaa tttgccagtg atattgcccc
    16021 cttcctgtgt catcccaatt tatggatacg ttatggtgcc gtgggattta tcacagtggt
    16081 agctcgtcaa ataagtacag ctgatgtcta ctgtaaactg atgccttatc ttgacccata
    16141 tattacccaa ccaataatac agattgaaag aaaacttgtt ctgctcagtg ttttaaagga
    16201 accagtaagt cgttctatat ttgattatgc tttgaggtct aaagatatta ctagcttgtt
    16261 cagacatctt cacatgcgtc agaagaaacg aaatggttct cttcccgact gccctccgcc
    16321 agaggatcct gccatagcac agcttctgaa gaagttgctc tcacagggaa tgacagagga
    16381 agaggaagac aaacttctgg cactgaaaga cttcatgatg aaatctaata aagcaaaggc
    16441 caatatagtg gaccagagcc atcttcatga tagtagtcag aaaggtgtaa ttgacttggc
    16501 agctttaggc ataactggga gacaagttga tcttgttaaa accaaacaag aaccagatga
    16561 caaacgggcc agaaaacatg taaaacaaga ctcaaatgta aatgaagaat ggaaaagcat
    16621 gtttgggtca ctggacccac caaacatgcc acaggcccta cctaaaggga gtgatcagga
    16681 ggtgattcag actgggaaac ctcctcgttc cgagtcctct gctggcattt gtgtcccttt
    16741 gtcaacttct tcacaggttc cagaagtgac aactgtccaa aataaaaaac cagtaatacc
    16801 ggttttaagt agtacaatct taccatccac ctatcagatt cgaattacaa cttgtaaaac
    16861 tgaacttcag caactcatcc agcaaaagcg ggagcagtgc aatgctgaga gaatagctaa
    16921 gcagatgatg gaaaatgctg aatgggagag taaaccacca ccacctggat ggcgtcctaa
    16981 agggctgtta gttgcccatc ttcatgagca taaatctgct gtgaatcgaa ttagagtctc
    17041 tgatgaacac tcactttttg caacatgttc aaatgatggc acagtgaaaa tctggaacag
    17101 tcaaaagatg gaggggaaga ccaccactac cagatctatt cttacataca gccgaattgg
    17161 aggacgagtc aagacgctca cattctgcca aggctcccac tatttagcca tagcatctga
    17221 taatggtgct gtccagcttc ttggaattga ggcttctaag ctgcccaagt ctcctaaaat
    17281 ccatcctcta caaagcagaa ttctagatca gaaggaggac ggttgtgttg tggatatgca
    17341 tcacttcaac tctggagcac agtctgttct tgcctatgcc actgtgaatg gctctctggt
    17401 tggctgggac cttaggtctt caagcaatgc gtggacttta aagcatgatt taaagtcggg
    17461 cctcatcact tcctttgctg tggacatcca ccaatgctgg ctctgcattg gtacaagcag
    17521 tggcaccatg gcttgttggg acatgaggtt ccagttgcca atttcaagtc actgtcatcc
    17581 ttccagggct cgaatcagac gcctctcaat gcaccctctg tatcagtcct gggtgattgc
    17641 agctgttcag ggcaacaacg aagtgtccat gtgggacatg gagactggtg acagaagatt
    17701 tactctctgg gccagcagtg caccaccact ttctgaatta cagccttctc ctcatagcgt
    17761 ccatggtatc tactgtagtc ctgcagatgg aaatcctatc ctactaacag ctggctcaga
    17821 tatgaaaata aggttttggg acttggctta cccagaaagg tcctatgttg ttgcaggaag
    17881 tactagttcc ccatctgtgt cctactacag gaaaataatt gaaggcactg aagttgtcca
    17941 ggaaattcag aataagcaga aagtaggacc aagtgatgac acccctcgaa ggggcccaga
    18001 gtccctgccc gtgggacatc atgacatcat cactgatgtc gccacattcc agaccacaca
    18061 gggcttcatc gtaactgctt ctagagatgg gattgtgaag gtgtggaaat aactcgagtc
    18121 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
    18181 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
    18241 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
    18301 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
    18361 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
    18421 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
    18481 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
    18541 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
    18601 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
    18661 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
    18721 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
    18781 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
    18841 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
    18901 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
    18961 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
    19021 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
    19081 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
    19141 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
    19201 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
    19261 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
    19321 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
    19381 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
    19441 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
    19501 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
    19561 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
    19621 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
    19681 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
    19741 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
    19801 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    19861 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    19921 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
    19981 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
    20041 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
    20101 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
    20161 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
    20221 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
    20281 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    20341 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    20401 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    20461 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    20521 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    20581 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    20641 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    20701 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    20761 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    20821 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    20881 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc
    20941 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    21001 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    21061 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    21121 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    21181 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    21241 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    21301 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    21361 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    21421 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    21481 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    21541 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    21601 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    21661 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    21721 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    21781 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    21841 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    21901 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    21961 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    22021 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    22081 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctga
//
