LOCUS       Exported               20833 bp ds-DNA     circular SYN 30-MAR-2014
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Serial Cloner Genbank Format  SerialCloner_Type=DNA  
            SerialCloner_Comments= - Infusion of : pYO386(EGFP+Beclin+hsVPS34+h 
            [17015  nt] : (MluI) to the PCR Fragment obtained using the primers 
            and   and matrix DNA [3844  nt] :  SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     source          1..20833
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    236..962
                     /label=CMV promoter
                     /label=CMV promoter(2)
     misc_feature    808..814
                     /label=TATA
                     /label=TATA(3)
     misc_feature    824..863
                     /label=x2 TetO2
                     /label=x2 TetO2(2)
     misc_feature    971..987
                     /label=MCS(4)
     CDS             994..3090
                     /label=UVRAG (no TGA)
     misc_feature    994..1021
                     /label=HsUVRAG
     misc_feature    1647..1671
                     /label=YOo53 f
     misc_feature    3092..3096
                     /label=BamH1
     misc_feature    3097..3120
                     /label=Prescission site
     misc_feature    3124..3147
                     /label=strep-tagII
     misc_feature    3124..3147
                     /label=strept tag
     misc_feature    3184..3207
                     /label=strep-tagII(1)
     misc_feature    3184..3207
                     /label=Strept tag
     misc_feature    3229..3249
                     /label=TEV(1)
     misc_feature    3229..3249
                     /label=TEV
                     /label=TEV(3)
     misc_feature    3271..3291
                     /label=TEV
     misc_feature    3271..3291
                     /label=TEV
                     /label=TEV(2)
     misc_feature    3292..3726
                     /label=x2 Protein A
     misc_feature    3292..3726
                     /label=2xProteinA (TGA)
     misc_feature    3770..3994
                     /label=PolA
                     /label=PolA(3)
     misc_feature    4059..4689
                     /label=CMV promoter
     misc_feature    4630..4636
                     /label=TATA
                     /label=TATA(4)
     misc_feature    4689..4711
                     /label=T7 pro
     primer_bind     4689..4708
                     /label=T7
     misc_feature    4716..4802
                     /label=MCS
     misc_feature    4803..5522
                     /label=EGFP coding
     misc_feature    5523..5528
                     /label=MCS(1)
     primer_bind     complement(5533..5550)
                     /label=SP6
     misc_feature    5552..5783
                     /label=bGH PolyA site
     misc_feature    5576..5800
                     /label=PolA
                     /label=PolA(4)
     misc_feature    5836..6562
                     /label=CMV promoter
                     /label=CMV promoter(3)
     misc_feature    6408..6414
                     /label=TATA
     misc_feature    6424..6463
                     /label=x2 TetO2
                     /label=x2 TetO2(3)
     misc_feature    6571..6587
                     /label=MCS(4)
                     /label=MCS(4)(1)
     misc_feature    6594..7946
                     /label=Beclin
     misc_feature    7990..8214
                     /label=PolA
     misc_feature    8253..8979
                     /label=CMV promoter(1)
     misc_feature    8825..8831
                     /label=TATA
                     /label=TATA(1)
     misc_feature    8841..8880
                     /label=x2 TetO2
     misc_feature    8988..9004
                     /label=MCS(4)
                     /label=MCS(4)(2)
     misc_feature    9011..11674
                     /label=HsVps34
     misc_feature    9011..9013
                     /label=ATG start
     misc_feature    9533..9557
                     /label=LZo110
     misc_feature    10031..10055
                     /label=LZO108
     misc_feature    10582..10605
                     /label=LZo109
     misc_feature    11719..11943
                     /label=PolA
                     /label=PolA(1)
     misc_feature    11982..12708
                     /label=CMV promoter(1)
                     /label=CMV promoter(1)(1)
     misc_feature    12554..12560
                     /label=TATA
                     /label=TATA(2)
     misc_feature    12570..12609
                     /label=x2 TetO2
                     /label=x2 TetO2(1)
     misc_feature    12717..12733
                     /label=MCS(4)
                     /label=MCS(4)(3)
     misc_feature    12734..16810
                     /label=hsVPS15
     misc_feature    12734..12736
                     /label=ATG start(1)
     misc_feature    12876..12895
                     /label=LZo115r
     misc_feature    13185..13204
                     /label=Synthesised primer 1
     misc_feature    13185..13204
                     /label=OP0635
     misc_feature    13918..13936
                     /label=LZo14
     misc_feature    14303..14331
                     /label=SAM097F
     misc_feature    14303..14331
                     /label=sam097(542-842)
     misc_feature    14303..14331
                     /label=SAM098R
     misc_feature    14303..14331
                     /label=sam098(182-512)
     misc_feature    15628..15649
                     /label=(LZ011)R
     misc_feature    15628..15649
                     /label=LZo15 R
     misc_feature    16208..16235
                     /label=sam0100(869-1148)
     misc_feature    16208..16235
                     /label=SAM0100R
     misc_feature    16208..16235
                     /label=SAM099F
     misc_feature    16811..16836
                     /label=MCS(5)
     misc_feature    16854..17078
                     /label=PolA
                     /label=PolA(2)
     rep_origin      17141..17447
                     /label=F1 ori
     primer_bind     complement(18679..18699)
                     /label=M13-rev
     misc_binding    complement(18705..18727)
                     /label=LacO
     CDS             complement(19844..20503)
                     /label=AmpR
ORIGIN
        1 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
       61 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
      121 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
      181 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
      241 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      301 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      361 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      421 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      481 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      541 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      601 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      661 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      721 aaccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      781 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctccctat cagtgataga
      841 gatctcccta tcagtgatag agatcgtcga cgagctcgtt tagtgaaccg tcagatcgcc
      901 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
      961 cggactctag cgtttaaact taagcttggt accatgagcg cctccgcgtc ggtcgggggc
     1021 caggtccccc agccaccccc gggcccggcc gctgctctgc ctcccggttc tgccgcgcgg
     1081 gccctgcatg tggagctgcc gtctcagcag cggcgtcttc gacatcttcg gaacattgct
     1141 gcccggaaca ttgttaatag aaatggccat cagctccttg atacctactt tacacttcac
     1201 ttgtgtagta ctgaaaagat atataaagaa ttttatagaa gtgaagtgat taagaattcc
     1261 ttgaatccca cgtggcgaag tctcgatttt ggaattatgc cagaccgtct tgatacatct
     1321 gtgtcttgtt tcgtggtgaa gatatggggt ggaaaggaga acatctacca gctgttgatt
     1381 gaatggaaag tctgtttgga tgggctgaaa tacttgggtc agcagattca tgcccgaaac
     1441 caaaatgaaa taatttttgg gctgaatgat ggatactatg gtgctccatt tgaacataag
     1501 ggttattcaa atgctcagaa gactattctt ctgcaggtgg atcagaactg tgttcgcaat
     1561 tcttacgatg tcttctcttt gctacggctt catagagccc agtgtgcaat taaacagact
     1621 caggtaactg ttcagaaaat tggaaaggaa attgaagaaa aactaagact cacatctaca
     1681 agcaatgaac tgaaaaaaaa aagtgaatgc ctgcagttaa aaattttggt gcttcagaat
     1741 gaactggaac ggcagaagaa agctttggga cgggaggtgg cattactgca taagcaacaa
     1801 attgcattac aagacaaagg aagtgcattt tcagctgagc acctcaaact tcaactccag
     1861 aaggaatccc taaatgagct gaggaaggag tgcactgcaa aaagagaact cttcttgaag
     1921 actaatgctc agttgacaat tcgttgcagg cagttactct ctgagctttc ctacatttac
     1981 cctattgatt tgaatgaaca taaggattac tttgtatgcg gtgtcaagtt gcctaattct
     2041 gaggacttcc aagcaaaaga tgatggaagc attgctgttg cccttggtta tactgcacat
     2101 ctggtctcca tgatttcctt tttcctacaa gtgcccctca gatatcctat aattcataag
     2161 gggtctagat caacaatcaa agacaatatc aatgacaaac tgacggaaaa ggagagagag
     2221 tttccactgt atccaaaagg aggggagaag ttgcagtttg attatggtgt ctatcttctg
     2281 aacaaaaata tagcacagct aagatatcaa catggactag ggactccaga cttgcggcaa
     2341 acccttccca acctgaaaaa cttcatggag catggactaa tggtcaggtg tgacagacat
     2401 cacacctcca gtgcaatccc tgttcctaag agacaaagct ccatatttgg gggtgcagat
     2461 gtaggcttct ctggggggat cccttcacca gacaaaggac atcgaaaacg ggccagctct
     2521 gagaatgaga gacttcagta caaaacccct cctcccagtt acaactcagc attagcccag
     2581 cctgtgacca ccgtcccctc catgggagag accgagagaa agataacatc tctatcctcc
     2641 tccttggata cctccttgga cttctccaaa gaaaacaaga aaaaaggaga ggatctagtt
     2701 ggcagcttaa acggaggcca cgcgaatgtg caccctagcc aagaacaagg agaagccctc
     2761 tccgggcacc gggccacagt caatggcact ctcctaccca gcgagcaggc cgggtccgcc
     2821 agtgtccagc ttccaggcga gttccaccca gtctcagaag ctgagctctg ctgtactgtg
     2881 gagcaagcag aagaaatcat cgggctggaa gccacaggtt tcgcctcagg tgatcagcta
     2941 gaagcattta actgcatccc agtggacagt gctgtggcag tagagtgtga cgaacaagtt
     3001 ctgggagaat ttgaagagtt ctcccgaagg atctatgcac tgaatgaaaa cgtatccagc
     3061 ttccgccggc cgcgcaggag ttccgataag agatccctgg aagttctgtt ccaggggcca
     3121 ggctggagcc acccgcagtt cgaaaaaggt tctgcgggat cagctgccgg atcgggagcg
     3181 ggttggagcc acccgcagtt cgagaaatct agaccaacta ctgcctctga aaatttgtac
     3241 tttcaaggcg agttgaagac cactgcttct gaaaacttgt acttccaagg tgagttactc
     3301 gagggtgaat tgaagactgc tgctttggct caacacgacg aagctgttga taataagttc
     3361 aataaggaac agcaaaacgc attctacgag attttgcatt tgccaaattt gaacgaggag
     3421 caacgtaacg cttttattca atccttgaag gatgacccat cccaatctgc caacttgtta
     3481 gctgaagcca agaagttaaa cgacgctcaa gccccaaaag ttgacaataa gtttaacaag
     3541 gaacaacaaa acgcctttta cgaaattttg cacttgccaa acttgaacga agaacaaaga
     3601 aacgctttca ttcaatcttt gaaggacgac ccatctcaat ctgctaattt gttggctgaa
     3661 gctaagaagt tgaacggtgc tcaagctcca aaggttgacg ctaactctgc tggtaagtct
     3721 acttgagtcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     3781 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     3841 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     3901 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     3961 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     4021 ggctctaggc gcgttgacat tgattattga ctagcgcgtt gacattgatt attgactagt
     4081 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     4141 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     4201 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     4261 gtggactatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     4321 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     4381 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     4441 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     4501 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     4561 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     4621 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
     4681 atcgaaatta atacgactca ctatagggag acccaagctt ggtaccgagc tcggatccac
     4741 tagtaacggc cgccagtgtg ctggaattct gcagatatcc atcacactgg cggccgctcg
     4801 agatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     4861 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     4921 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     4981 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     5041 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     5101 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     5161 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     5221 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     5281 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     5341 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     5401 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     5461 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     5521 aagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg
     5581 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     5641 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     5701 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     5761 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     5821 agctggggct ctagggttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     5881 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     5941 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     6001 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     6061 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     6121 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     6181 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     6241 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     6301 tcaatgggag tttgttttgg aaccaaaatc aacgggactt tccaaaatgt cgtaacaact
     6361 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     6421 ctctccctat cagtgataga gatctcccta tcagtgatag agatcgtcga cgagctcgtt
     6481 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
     6541 accgggaccg atccagcctc cggactctag cgtttaaact taagcttggt accatggaag
     6601 ggtctaagac gtccaacaac agcaccatgc aggtgagctt cgtgtgccag cgctgcagcc
     6661 agcccctgaa actggacacg agtttcaaga tcctggaccg tgtcaccatc caggaactca
     6721 cagctccatt acttaccaca gcccaggcga aaccaggaga gacccaggag gaagagacta
     6781 actcaggaga ggagccattt attgaaactc ctcgccagga tggtgtctct cgcagattca
     6841 tccccccagc caggatgatg tccacagaaa gtgccaacag cttcactctg attggggagg
     6901 catctgatgg cggcaccatg gagaacctca gccgaagact gaaggtcact ggggaccttt
     6961 ttgacatcat gtcgggccag acagatgtgg atcacccact ctgtgaggaa tgcacagata
     7021 ctcttttaga ccagctggac actcagctca acgtcactga aaatgagtgt cagaactaca
     7081 aacgctgttt ggagatctta gagcaaatga atgaggatga cagtgaacag ttacagatgg
     7141 agctaaagga gctggcacta gaggaggaga ggctgatcca ggagctggaa gacgtggaaa
     7201 agaaccgcaa gatagtggca gaaaatctcg agaaggtcca ggctgaggct gagagactgg
     7261 atcaggagga agctcagtat cagagagaat acagtgaatt taaacgacag cagctggagc
     7321 tggatgatga gctgaagagt gttgaaaacc agatgcgtta tgcccagacg cagctggata
     7381 agctgaagaa aaccaacgtc tttaatgcaa ccttccacat ctggcacagt ggacagtttg
     7441 gcacaatcaa taacttcagg ctgggtcgcc tgcccagtgt tcccgtggaa tggaatgaga
     7501 ttaatgctgc ttggggccag actgtgttgc tgctccatgc tctggccaat aagatgggtc
     7561 tgaaatttca gagataccga cttgttcctt acggaaacca ttcatatctg gagtctctga
     7621 cagacaaatc taaggagctg ccgttatact gttctggggg gttgcggttt ttctgggaca
     7681 acaagtttga ccatgcaatg gtggctttcc tggactgtgt gcagcagttc aaagaagagg
     7741 ttgagaaagg cgagacacgt ttttgtcttc cctacaggat ggatgtggag aaaggcaaga
     7801 ttgaagacac aggaggcagt ggcggctcct attccatcaa aacccagttt aactctgagg
     7861 agcagtggac aaaagctctc aagttcatgc tgacgaatct gaagtggggt ctggcttggg
     7921 tgtcctctca attttataac aaatgagtcg agtctagagg gcccgtttaa acccgctgat
     7981 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     8041 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     8101 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     8161 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     8221 aggcggaaag aaccagctgg ggctctaggc gcgttgacat tgattattga ctagttatta
     8281 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     8341 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     8401 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     8461 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     8521 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     8581 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     8641 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     8701 tctccacccc attgacgtca atgggagttt gttttggaac caaaatcaac gggactttcc
     8761 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     8821 ggtctatata agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga
     8881 tcgtcgacga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     8941 tgacctccat agaagacacc gggaccgatc cagcctccgg actctagcgt ttaaacttaa
     9001 gcttggtacc atgggggaag cagagaagtt tcactacatc tatagttgtg acctggatat
     9061 caacgtccag cttaagatag gaagcttgga agggaagaga gaacaaaaga gttataaagc
     9121 tgtcctggaa gacccaatgt tgaagttctc aggactatat caagagacat gctctgatct
     9181 ttatgttact tgtcaagttt ttgcagaagg gaagcctttg gccttgccag tgagaacatc
     9241 ctacaaagca tttagtacaa gatggaactg gaatgaatgg ctgaaactac cagtaaaata
     9301 ccctgacctg cccaggaatg cccaagtggc cctcaccatc tgggatgtgt atggtcccgg
     9361 aaaagcagtg cctgtaggag gaacaacggt ttcgctcttt ggaaaatacg gcatgtttcg
     9421 ccaagggatg catgacttga aagtctggcc taatgtagaa gcagatggat cagaacccac
     9481 aaaaactcct ggcagaacaa gtagcactct ctcagaagat cagatgagcc gtcttgccaa
     9541 gctcaccaaa gctcatcgac aaggacacat ggtgaaagta gattggctgg atagattgac
     9601 atttagagaa atagaaatga taaatgagag tgaaaaacga agttctaatt tcatgtacct
     9661 gatggttgaa tttcgatgtg tcaagtgtga tgataaggaa tatggtattg tttattatga
     9721 aaaggacggt gatgaatcat ctccaatttt aacaagtttt gaattagtga aagttcctga
     9781 cccccagatg tctatggaga atttagttga gagcaaacac cacaagcttg cccggagttt
     9841 aagaagtgga ccttctgacc acgatctgaa acccaatgct gccacgagag atcagttaaa
     9901 tattattgtg agttatccac caaccaagca acttacctat gaagaacaag atcttgtttg
     9961 gaagtttaga tattatctta cgaatcaaga aaaagccttg acaaaattct tgaaatgtgt
    10021 taattgggat ctacctcaag aggccaaaca ggccttggaa cttctgggaa aatggaagcc
    10081 gatggatgta gaggactcct tggagctgtt atcctctcat tacaccaacc caactgtgag
    10141 gcgttatgct gttgcccggt tgcgacaggc cgatgatgag gatttgttga tgtacctatt
    10201 acaattggtc caggctctca aatatgaaaa ttttgatgat ataaagaatg gattggaacc
    10261 taccaagaag gatagtcaga gttcagtgtc agaaaatgtg tcaaattctg gaataaattc
    10321 tgcagaaata gatagctccc aaattataac cagccccctt ccttcagtct cttcacctcc
    10381 tcctgcatca aaaacaaaag aagttccaga tggcgaaaat ctggaacaag atctctgtac
    10441 cttcttgata tcgagagcct gcaaaaactc aacactggct aattatttat actggtatgt
    10501 gatagtggaa tgtgaagatc aagatactca gcagagagat ccaaagaccc atgagatgta
    10561 cttgaacgta atgagaagat tcagccaagc attgttgaag ggtgataagt ctgtcagagt
    10621 tatgcgttct ttgctggctg cacaacagac atttgtagat cggttggtgc atctaatgaa
    10681 ggcagtacaa cgcgaaagtg gaaatcgtaa gaaaaagaat gagagactac aggcattgct
    10741 tggagataat gaaaagatga atttgtcaga tgtggaactt atcccgttgc ctttagaacc
    10801 ccaagtgaaa attagaggaa taattccgga aacagctaca ctgtttaaaa gtgcccttat
    10861 gcctgcacag ttgtttttta agacggaaga tggaggcaaa tatccagtta tatttaagca
    10921 tggagatgat ttacgtcaag atcaacttat tcttcaaatc atttcactca tggacaagct
    10981 gttacggaaa gaaaatctgg acttgaaatt gacaccttat aaggtgttag ccaccagtac
    11041 aaaacatggc ttcatgcagt ttatccagtc agttcctgtg gctgaagttc ttgatacaga
    11101 gggaagcatt cagaactttt ttagaaaata tgcaccaagt gagaatgggc caaatgggat
    11161 tagtgctgag gtcatggaca cttacgttaa aagctgtgct ggatattgcg tgatcaccta
    11221 tatacttgga gttggagaca ggcacctgga taaccttttg ctaacaaaaa caggcaaact
    11281 cttccacata gactttggat atattctggg tcgtgatcca aagcctcttc ctccaccaat
    11341 gaagctgaat aaagaaatgg tagaaggaat ggggggcaca cagagtgagc agtaccaaga
    11401 gttccgtaaa cagtgttaca cggctttcct ccacctgcga aggtattcta atctgatttt
    11461 gaacttgttt tccttgatgg ttgatgcaaa cattccagat attgcacttg aaccagataa
    11521 aactgtgaaa aaggttcagg ataaattccg cttagacctg tcggatgaag aggctgtgca
    11581 ttacatgcag agtctgattg atgagagtgt ccatgctctt tttgctgcag tggtggaaca
    11641 gattcacaag tttgcccagt actggcgcaa atgagtcgag gtctagaggg cccgtttaaa
    11701 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
    11761 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
    11821 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
    11881 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
    11941 tggcttctga ggcggaaaga accagctggg gctctaggcg cgttgacatt gattattgac
    12001 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
    12061 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
    12121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
    12181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
    12241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
    12301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
    12361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
    12421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggaacc aaaatcaacg
    12481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
    12541 acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc tccctatcag
    12601 tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
    12661 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga ctctagcgtt
    12721 taaacttaag cttatgggaa atcagcttgc tggcattgct ccctcccaga tcctttctgt
    12781 agagagttat ttttcagata ttcatgactt tgaatatgat aaaagcctgg ggagtactcg
    12841 gttttttaaa gttgctcgag ccaagcaccg agaaggcctg gtcgttgtga aggtttttgc
    12901 aattcaggat cccacattgc ctttaaccag ctataaacaa gagctggagg aactgaaaat
    12961 caggcttaat tctgcacaga attgtctacc tttccagaaa gcatcagaaa aagcatctga
    13021 gaaagcagct atgctcttta ggcagtatgt gcgagacaat ctctatgatc gcatcagtac
    13081 ccgtccattc ttgaataaca ttgagaagcg ctggattgct ttccagatcc tgacagctgt
    13141 ggaccaagca cacaaatctg gagttcgtca tggggacatc aagactgaga atgtgatggt
    13201 caccagttgg aattgggttc ttctaactga ttttgccagt tttaagccca cttatcttcc
    13261 agaagacaac ccggcagatt tcaattattt ctttgacaca tcacggagga gaacttgcta
    13321 tattgctcct gaacgttttg ttgatggtgg gatgtttgcc actgagttag aatatatgag
    13381 agatccttca actccgcttg tagacttaaa tagcaatcag agaacaagag gagagttgaa
    13441 gagagcaatg gacatctttt cagcaggttg tgtgatagct gagcttttta cagaaggtgt
    13501 accattattt gatctctctc aacttttggc ttatagaaat ggacattttt tccctgaaca
    13561 agtgctaaat aaaattgaag atcacagtat cagagaattg gtaactcaga tgattcaccg
    13621 tgagccagat aaacgtttag aggcagaaga ttacttaaaa cagcagcgtg gcaatgcctt
    13681 tcctgaaata ttttacactt ttcttcagcc ctacatggcc cagtttgcca aggaaacgtt
    13741 tctttctgca gatgagcgta ttctggttat acggaaggat ttgggcaaca ttattcacaa
    13801 tctctgtgga catgatctgc cagaaaaagc cgaaggagag cctaaggaaa atgggctggt
    13861 tatcttggta tctgttataa catcctgcct acagaccctt aaatactgtg attccaaact
    13921 agctgctttg gaactgattc ttcatttggc tccaagatta agtgttgaaa tccttttgga
    13981 tcgtattact ccatatcttt tgcatttcag caatgactct gttcctaggg tgagggctga
    14041 agccttgagg acgttgacca aagttcttgc tctcgtcaaa gaggttcctc gtaatgatat
    14101 caatatttat ccggaataca ttctgccagg catagcccac ttagcccaag atgatgctac
    14161 tatcgttaga ctagcctatg ctgaaaacat agctctgctg gcagaaacag ctctgagatt
    14221 cctggaatta gtacagttaa aaaatcttaa tatggaaaat gaccccaata atgaagaaat
    14281 agatgaggtt acacatccaa atggaaatta tgacacagag ctgcaagcct tacatgaaat
    14341 ggtccagcag aaagttgtta ctttgctaag tgaccctgaa aatattgtaa aacaaacctt
    14401 gatggaaaat ggaataacac ggctgtgtgt attctttgga cgtcagaaag ccaacgatgt
    14461 tttgttgtcc cacatgatta ctttcctaaa tgataagaat gattggcatc tacgtggagc
    14521 attttttgat agtatagttg gtgttgctgc ctatgttggc tggcaaagct cctcaattct
    14581 caagcctctg ctgcaacaag gtcttagtga tgctgaggaa tttgtcattg tgaaagctct
    14641 ttatgccctt acttgtatgt gccagttagg actgctacaa aaaccccatg tttacgaatt
    14701 tgccagtgat attgccccct tcctgtgtca tcccaattta tggatacgtt atggtgccgt
    14761 gggatttatc acagtggtag ctcgtcaaat aagtacagct gatgtctact gtaaactgat
    14821 gccttatctt gacccatata ttacccaacc aataatacag attgaaagaa aacttgttct
    14881 gctcagtgtt ttaaaggaac cagtaagtcg ttctatattt gattatgctt tgaggtctaa
    14941 agatattact agcttgttca gacatcttca catgcgtcag aagaaacgaa atggttctct
    15001 tcccgactgc cctccgccag aggatcctgc catagcacag cttctgaaga agttgctctc
    15061 acagggaatg acagaggaag aggaagacaa acttctggca ctgaaagact tcatgatgaa
    15121 atctaataaa gcaaaggcca atatagtgga ccagagccat cttcatgata gtagtcagaa
    15181 aggtgtaatt gacttggcag ctttaggcat aactgggaga caagttgatc ttgttaaaac
    15241 caaacaagaa ccagatgaca aacgggccag aaaacatgta aaacaagact caaatgtaaa
    15301 tgaagaatgg aaaagcatgt ttgggtcact ggacccacca aacatgccac aggccctacc
    15361 taaagggagt gatcaggagg tgattcagac tgggaaacct cctcgttccg agtcctctgc
    15421 tggcatttgt gtccctttgt caacttcttc acaggttcca gaagtgacaa ctgtccaaaa
    15481 taaaaaacca gtaataccgg ttttaagtag tacaatctta ccatccacct atcagattcg
    15541 aattacaact tgtaaaactg aacttcagca actcatccag caaaagcggg agcagtgcaa
    15601 tgctgagaga atagctaagc agatgatgga aaatgctgaa tgggagagta aaccaccacc
    15661 acctggatgg cgtcctaaag ggctgttagt tgcccatctt catgagcata aatctgctgt
    15721 gaatcgaatt agagtctctg atgaacactc actttttgca acatgttcaa atgatggcac
    15781 agtgaaaatc tggaacagtc aaaagatgga ggggaagacc accactacca gatctattct
    15841 tacatacagc cgaattggag gacgagtcaa gacgctcaca ttctgccaag gctcccacta
    15901 tttagccata gcatctgata atggtgctgt ccagcttctt ggaattgagg cttctaagct
    15961 gcccaagtct cctaaaatcc atcctctaca aagcagaatt ctagatcaga aggaggacgg
    16021 ttgtgttgtg gatatgcatc acttcaactc tggagcacag tctgttcttg cctatgccac
    16081 tgtgaatggc tctctggttg gctgggacct taggtcttca agcaatgcgt ggactttaaa
    16141 gcatgattta aagtcgggcc tcatcacttc ctttgctgtg gacatccacc aatgctggct
    16201 ctgcattggt acaagcagtg gcaccatggc ttgttgggac atgaggttcc agttgccaat
    16261 ttcaagtcac tgtcatcctt ccagggctcg aatcagacgc ctctcaatgc accctctgta
    16321 tcagtcctgg gtgattgcag ctgttcaggg caacaacgaa gtgtccatgt gggacatgga
    16381 gactggtgac agaagattta ctctctgggc cagcagtgca ccaccacttt ctgaattaca
    16441 gccttctcct catagcgtcc atggtatcta ctgtagtcct gcagatggaa atcctatcct
    16501 actaacagct ggctcagata tgaaaataag gttttgggac ttggcttacc cagaaaggtc
    16561 ctatgttgtt gcaggaagta ctagttcccc atctgtgtcc tactacagga aaataattga
    16621 aggcactgaa gttgtccagg aaattcagaa taagcagaaa gtaggaccaa gtgatgacac
    16681 ccctcgaagg ggcccagagt ccctgcccgt gggacatcat gacatcatca ctgatgtcgc
    16741 cacattccag accacacagg gcttcatcgt aactgcttct agagatggga ttgtgaaggt
    16801 gtggaaataa ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
    16861 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
    16921 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
    16981 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
    17041 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
    17101 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
    17161 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
    17221 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
    17281 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
    17341 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
    17401 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
    17461 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
    17521 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
    17581 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
    17641 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
    17701 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
    17761 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
    17821 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    17881 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
    17941 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
    18001 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
    18061 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
    18121 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
    18181 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
    18241 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
    18301 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
    18361 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
    18421 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
    18481 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
    18541 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    18601 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
    18661 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
    18721 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    18781 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    18841 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    18901 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    18961 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    19021 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    19081 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    19141 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    19201 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    19261 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    19321 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    19381 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    19441 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    19501 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    19561 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    19621 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    19681 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    19741 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    19801 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    19861 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    19921 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    19981 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    20041 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    20101 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    20161 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    20221 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    20281 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    20341 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    20401 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    20461 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    20521 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    20581 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    20641 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    20701 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    20761 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    20821 aagtgccacc tga
//
